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Citations for Bio-Rad :
8751 - 8800 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... boiled for 10 min at 95°C and examined by western blot using 4-15% Mini-PROTEAN TGX precast protein gels (BioRad #4561083). After blocking step with 10% non-fat milk for 1h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated by SDS-PAGE on 16% acrylamide gels immobilized by transfer to a PVDF membrane (Cat. #1620177, Bio-Rad) using a Trans-Blot Turbo Transfer System (Trans-Blot ®TurboTM ...
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bioRxiv - Microbiology 2024Quote: ... protein concentrations were measured using the BCA assay and 20 μg of proteins from whole cell lysates were resolved on pre-cast 12% tris-glycine PAGE (Bio-Rad) followed by transfer to a LF-PVDF membrane using a Bio-Rad semi dry transfer apparatus ...
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bioRxiv - Neuroscience 2024Quote: ... The protein chemiluminescence signal was obtained and visualized with the ChemiDocTM Touch Gel Imaging System (Bio-Rad Laboratories, Hercules, CA, USA). After FasII or NCAM1 detection ...
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bioRxiv - Molecular Biology 2024Quote: ... as well as either Coomassie Blue staining (for the TGX Precast Protein Gel) or the ChemiDoc Touch Imaging System (Bio-Rad) (for the TGX Stain-Free™ Protein Gel).
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bioRxiv - Neuroscience 2024Quote: ... Samples were split and electrotransferred using 4-20% Mini-PROTEAN TGX Stain-Free Protein Gels and the PVDF membrane Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were immediately lysed in ice cold Radioimmunoprecipitation Assay (RIPA) buffer and protein was quantified using a Bradford Assay (Bio-Rad), per manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... Samples were centrifuged at 15700 g for 30 min at 4°C and protein concentration was determined by Bradford assay (Bio-Rad). 600 µM of GppNHp Rab8a1-176 was titrated into the cell containing 30 µM bMERB domain and for full-length Mical1 ...
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bioRxiv - Physiology 2024Quote: ... Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min and the separated proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane using a wet transfer cell (Bio-Rad) at 100 mAmps (4°C overnight) ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were then transferred to polyvinylidene difluoride membranes using NuPAGE Transfer Buffer using a Trans-Blot Turbo Transfer System (Bio-Rad) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were then transferred from the gel onto a nitrocellulose membrane using a semi-dry method (BioRad Trans-Blot Turbo). The membrane was blocked with 5% non-fat dry milk in 1X TBST for 1 hour ...
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bioRxiv - Immunology 2024Quote: The pellet can be saved as a source of LPS and the soluble protein antigens were treated overnight with Affi-Prep Polymyxin Resin (Bio-Rad) to remove trace amounts of LPS remaining.
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bioRxiv - Cell Biology 2024Quote: ... using the BioRad Mixed Molecular Weight Protein Transfer setting (7 min, 1.3 A, 25 V) on the Trans-Blot Turbo Transfer System (Bio-Rad, 1704150) with Trans-Blot Turbo Transfer Buffer (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were separated by SDS-PAGE using 7.5% gels or Any kD Mini-PROTEAN TGX Precast Protein Gels (Biorad, cat. 4569033) and transferred to PVDF membranes using a Trans-Blot Turbo Transfer System (Biorad ...
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bioRxiv - Molecular Biology 2024Quote: ... for at least 30 min at 4 °C cleared by centrifugation and protein concentration was determined by Bradford assay (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... the cells were irradiated with 400 mJ/cm2 UV light to fixate the RNA-protein complexes by covalent cross-linking in the cells (GS Gene Linker UV Chamber, Bio-Rad); except for the control samples which were not UV-treated ...
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bioRxiv - Plant Biology 2024Quote: ... the samples were combined with SureBeads™ Protein G (10 μL in 50% incubation buffer slurry; Bio-Rad, Hercules, CA, USA). After 3 h of incubation at 4 °C with rotation ...
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bioRxiv - Neuroscience 2024Quote: ... 7µg of total protein was incubated in loading buffer as described above and loaded into AnykD Mini-PROTEAN TGX Precast Protein Gels (15 wells, 15uL; Bio-Rad). Electrophoresis and protein transfer were performed as above ...
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bioRxiv - Cell Biology 2024Quote: ... For western blotting the proteins were transferred to polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo RTA Mini PVDF Transfer Kit (Bio-Rad) and the Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were visualized using horseradish-peroxidase-conjugated secondary antibodies followed by chemiluminescence detection with ECL (Clarity Western ECL substrate, Bio-Rad).
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bioRxiv - Biophysics 2021Quote: ... We centrifuged the dialysis contents to remove any insoluble protein and then purified using cation exchange chromatography (BioRad UNOsphere Rapid S column) with an NaCl gradient ...
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bioRxiv - Biophysics 2021Quote: The removal of DDM in the protein solutions was done by fixing the protein concentration at 1 mg/ml and using 30 mg of BioBeads SM2 (Bio-Rad, CA) per mg of protein ...
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bioRxiv - Cell Biology 2020Quote: ... Equivalent amounts of total protein per lysate were loaded onto Criterion XT 4-12% Bis-Tris polyacrylamide gels (Bio-Rad, Hercules, CA), transferred onto PVDF membranes (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were boiled at 95°C for 5 minutes with Laemmli buffer and 20 μg of each was loaded into a 4-20% Mini-PROTEAN TGX Precast Protein Gel (BioRad, Hercules, CA). Gels were run at 100 V for 30-60 minutes and then transferred to PVDF membranes for 2 hours at 250 mV ...
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bioRxiv - Plant Biology 2020Quote: ... the recombinant protein in the filtrate was further purified by size exclusion chromatography (NGCTM chromatography system with EnrichTM SEC 650 column, BioRad, Munich, Germany) in 50 mM NaH2PO4 /Na2HPO4 ...
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bioRxiv - Developmental Biology 2021Quote: ... by immunoprecipitation of endogenous or endogenously Flag-tagged Piwi (eF-Piwi): The anti-Piwi antibody and Surebeads Protein A Magnetic bead (Bio-Rad, 1614013) were used to immunoprecipitated endogenous Piwi-piRNAs from witld type OSC ...
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bioRxiv - Microbiology 2022Quote: ... Brain homogenates were assayed at neat for all analytes and results were normalised to their total protein concentrations (Bio-Rad, Hercules, California).
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bioRxiv - Neuroscience 2021Quote: ... 20 μg of total cell proteins were run in a 12% SDS-polyacrylamide gel and blotted to the PVDF membrane (Bio-Rad, USA). Membranes were incubated overnight at 4 °C with mature BDNF primary antibody (ThermoFisher OSB00017W ...
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bioRxiv - Neuroscience 2021Quote: ... samples heat denatured and 20 µg of total protein was loaded per lane on 4-20% Tris-HCl polyacrylamide gels (BioRad, California, USA). After transfer to PVDF ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotinylated proteins were then eluted by boiling the magnetic beads in 30 μL 4 χ Laemmli sample buffer (Bio-Rad, Cat.# 1610747) supplemented with 20 mM DTT and 2 mM biotin.
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was incubated with primary antibody and horseradish peroxidase–conjugated secondary antibody, and then proteins were detected using the Pierce™ ECL Western Blotting Substrate (Thermo, #32209) by BIO-RAD ChemiDoc™ XRS+ system ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleaned samples were solubilized in the previous buffer described above and then quantified with the RCDC Protein Assay kit (Biorad, Hercules, CA). Ten μg of protein from each sample were digested in-solution using both Lys-C and Trypsin ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was detected using an ECL system (Amersham Pharmacia, Piscataway, NJ, USA) and was analysed using Quantity One software (BIO-RAD, USA).
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bioRxiv - Cell Biology 2022Quote: Protein homogenates (5 or 15 μg/lane) were size fractionated on 4–15% or 15% Criterion TGX gels (Biorad Laboratories, Oslo, Norway) and transferred to 0.45 μM PVDF-membranes (GE Healthcare) ...
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bioRxiv - Neuroscience 2019Quote: ... 5uL of the sample was loaded into 4-6% polyacrylamide gel along with Precision Plus Protein™ Dual Color Standard (#1610374S, Bio-Rad) and was run at 120 V ...
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bioRxiv - Molecular Biology 2019Quote: ... Denatured proteins (20-40 µg) were electrophoresed in 9% SDS-PAGE gels or MiniPROTEAN® TGX™ 4-15% Precast gels (BIORAD), transferred onto a nitrocellulose membrane and probed with specific antibodies ...
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bioRxiv - Biophysics 2019Quote: ... The concentration of the dialyzed protein was estimated using the Quick Start®Bradford Dye Reagent 1X (BIO-RAD Cat. #500-0205). The protein was stored at 4 °C before use.
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bioRxiv - Developmental Biology 2020Quote: ... 1-20μg of tissue protein to be analyzed were combined with equal parts 2X Laemmli sample buffer (Bio-Rad, catalog 161-0737) containing 10% β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2019Quote: ... 40µg of protein from each sample was loaded onto the 4– 15% Mini-PROTEAN® TGX™ Gel (BioRad, Catalog number: 4561083) for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Neuroscience 2019Quote: ... and 50 μg protein per sample were run on an SDS-PAGE gel and transferred to an Immun-Blot PVDF Membrane (Bio-Rad Laboratories) using standard protocols ...
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bioRxiv - Physiology 2019Quote: ... Diluted proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on a 4-20% gradient Criterion TGX Precast Midi Protein gel (Bio-Rad, CA), and subsequently transferred onto nitrocellulose membranes (Bio-Rad ...
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Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroidesbioRxiv - Genetics 2020Quote: Equal volumes of protein were loaded onto pre-cast BioRad Mini-Protean TGX precast Gel 4-20% (15-wells BioRad cat# 4568096). The gels were run according to the manufacturer’s guidelines (110Volts for 3 minutes ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... Proteins were separated on SDS-containing 8 to 12 % polyacrylamide gels and transferred to PVDF membranes (Bio-Rad Laboratories, Inc., Hercules, USA). After staining with primary and secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... 9 µL of each cell lysate and 14 µL of each secreted protein sample were run on a 4–20% Tris-glycine gel (Bio-Rad; 4561094) together with the Precision Plus Protein Dual Color Standards molecular weight marker (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: Electrophoretic analyses of viral proteins and equine formulations were performed in 4-20% SDS-PAGE gel Mini-PROTEAN TGX (BioRad; California, USA) and 7.5% SDS- polyacrylamide gels (SDS-PAGE) ...
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bioRxiv - Biochemistry 2020Quote: ... protein was eluted from the beads by boiling for 5 minutes at 95°C in Laemmli Sample Buffer (BioRad, catalog #161-0747). Proteins were stored at −80°C until western blotting.
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bioRxiv - Bioengineering 2021Quote: ... Produced protein was sequentially purified by affinity chromatography using Ni-NTA agarose beads and fast protein liquid chromatography (FPLC, NGC Quest 10 Chromatography system, Biorad, Hercules, CA). Protein fractions detected at λ = 280 were collected ...
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bioRxiv - Bioengineering 2020Quote: ... Tumors were homogenized and lysed using RIPA buffer and the total protein concentrations were determined using DC assay reagent (Bio-Rad Laboratories). Cell lysates (50 μg ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then loaded onto 4-15% Criterion™ TGX™ Precast Midi Protein Gels (12 + 2 wells, 45 μl) (Bio-Rad) and subjected to SDS-PAGE at 200 V for ~45 min in 10× Tris/Glycine/SDS running buffer (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... A gel slice corresponding to 100 to 150 kd was isolated (based on size markers in an adjacent lane; Precision Plus Protein™ Dual Color Standards from Bio-Rad). The gel slice was transferred to a D-Tube Dialyzer Midi Tube (EMD-Millipore) ...