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Citations for Bio-Rad :
8601 - 8650 of 10000+ citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Each sample was prepared for western blotting by diluting 20 μg of protein with 1 × Laemmli buffer (Bio-Rad #1610747) and ultrapure water (Invitrogen #10977015 ...
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bioRxiv - Biophysics 2021Quote: ... protein was further purified by a Hydroxyapatite column (HA) connected to an NGC Medium-Pressure Liquid Chromatography System (BIO-RAD). A gradient form 5 mM NaPi ...
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bioRxiv - Biochemistry 2021Quote: ... Whole cell lysate samples containing 4 μg of protein were fractionated using a 4–20% SDS-PAGE gel (Bio-Rad). Proteins were transferred to a 0.2 μm nitrocellulose membrane using a Trans-Blot Turbo transfer system (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... 50 μg protein from each sample were resolved in 10% SDS-polyacrylamide gel and transferred onto 0.2 μm nitrocellulose membranes (Bio-Rad). The nitrocellulose membranes were blocked in 5% nonfat dry milk in Tris-buffered saline containing 0.1% Tween 20 ...
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bioRxiv - Biophysics 2020Quote: ... proteins from the supernatant were separated by 12% SDS-PAGE and transferred onto a PVDF membrane (BioRad, Hercules, CA, USA). The Signal Enhancer HIKARI (Nacalai Tesque ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of protein (5–20 μg) were loaded into each lane and separated on 4–20% polyacrylamide tris glycine SDS gels (BioRad), then transferred to polyvinylideneifluoride membranes (BioRad) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred to polyvinylidene difluoride (PVDF) membranes and chemiluminescent signal was acquired with a Chemidoc Imaging System (Bio-Rad). Images were processed with the ImageLab software (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... and protein concentrations were equalized prior to boiling in the recommended sample buffers supplemented with 2.5% beta-mercaptoethanol (Bio-Rad). SDS-PAGE electrophoresis was performed using 4-12% gradient bis-tris gels or 16% tricine gels (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: Heat-induced protein denaturation curves were obtained using a CFX Connect Real-Time PCR system (Bio-Rad Laboratories, Hercules, CA) as described previously 99 ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were run on 7.5% Mini-PROTEAN gels for cytoplasmic proteins and 12.5% Mini-PROTEAN gels for histones (Bio-Rad, #4568025). Primary antibodies used ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were desalted into phosphate-buffered saline (PBS) pH 7.4 using Econo-Pac 10DG gravity flow chromatography columns (Bio-Rad). Absorbance ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were prepared at 25 µg protein/well with 2X laemmli loading buffer and 2-mercaptoethanol and run on pre-cast 4-15% gels (Biorad). Proteins were wet transferred onto PVDF membranes ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were denatured and proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on Mini-Protean 10% tris-glycine gels (BioRad). Precision plus protein Kaleidoscope molecular weight (MW ...
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bioRxiv - Cell Biology 2020Quote: ... 10 µg of total protein lysate was loaded on to an TGX Stain Free FastCast Acrylamide gel (BioRad, cat# 1610185) and transferred using an iBlot2 gel transfer device (Thermofisher scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Lyophilized proteins were dissolved in SDS-PAGE sample buffer and resolved by SDS-PAGE (BioRad precast 4-15 % gradient gel). The gel was stained with Simple Blue Safe Stain (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... The protein extracts were resolved on 4-15% Criterion™ Tris-HCl precast gels (#3450029, Bio-Rad Laboratories, CA, USA) and blotted onto PVDF membranes (#1704157 ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared at 12 000 rpm for 10 min at 4°C and protein concentration adjusted with Bradford assay (BioRad). The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amount of proteins was loaded onto 12% SDS-PAGE gel (acrylamide: bisacrylamide 29:1, cat# 161-0146, Bio-Rad) and after size separation blotted onto 0.2 μm nitrocellulose membrane (cat# 162-0112 ...
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bioRxiv - Genetics 2021Quote: ... Proteins (7 μl of extract) were separated on Laemmli SDS-PAGE gels and transferred to nitrocellulose membranes (Bio-Rad, 1620112). Membranes were stained with Ponceau-S for visualization ...
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bioRxiv - Genetics 2021Quote: ... and 10 µg protein were resolved by SDS-polyacrylamide gel electrophoresis and transferred to a 0.2 µm nitrocellulose membrane (Bio-Rad). The membrane was blocked in 5% skimmed milk powder in PBX (0.01% Triton X-100 in PBS ...
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bioRxiv - Genetics 2021Quote: ... Proteins were visualized using horseradish peroxidase-conjugated goat anti-rabbit secondary antibody conjugate (1:2000 dilution, Bio-Rad laboratories, #1706515) or horseradish peroxidase-conjugated goat anti-mouse secondary antibody conjugate (1:2000 dilution ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were prepared for mass spectrometry by disrupting total or immunopurified ribosomes in 1X Laemmli buffer and running on a 4-20% Mini-PROTEAN TGX Precast Protein Gel in 1X Tris/Glycine/SDS buffer (BioRad) for 5 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... 0.1 OD equivalents of the resulting sample was subjected to SDS-PAGE and the proteins were separated on 4-15% Mini-PROTEAN-TGX strain-free gels (BioRad). For subsequent immuno-blotting ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μg of total protein extracts were run on a gradient (15-4%) Polyacrylamide gel (MINIi-PROTEAN TGX Precast Gels, Biorad). Proteins were transferred on nitro-cellulose membrane and checked with Red Ponceau staining for efficient transfer ...
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bioRxiv - Molecular Biology 2021Quote: ... Denatured protein samples were then resolved in a 10% SDS-PAGE and transferred to a PVDF membrane (Bio-Rad, #1620177). Blots were probed by custom-designed Drosophila antibodies as previously described 6 diluted in PBS-0.1% Tween supplemented with 5% nonfat milk ...
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bioRxiv - Pathology 2022Quote: ... Appropriate total proteins from different lines were resolved on 10 % polyacrylamide gels and transferred to a nitrocellulose membrane (Bio-Rad). Anti-FLAG monoclonal antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... and equal amount of proteins were separated by SDS/PAGE on pre-cast 4-20% polyacrylamide gradient gels (Cat# 5671094, Biorad) and transferred to immune-blot PVDF membrane (Merck cat# IPVH00010) ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were resolved by gel electrophoresis on a 4 to 20% polyacrylamide gradient Mini-Protean TGX precast gel (Bio-Rad) and transferred to an Immobilon-P membrane (Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were separated at 175 V for 3-4 hours at 4°C and then transferred to nitrocellulose membrane (BioRad) by using a Criterion blot cell (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoreactivities of the blotted proteins were detected with Clarity Western ECL Substrate for Chemiluminescent Western Blot Detection (Bio-rad, U.S.A.) and ChemiDoc XRS+ system (Bio-rad ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were heated at 60°C and loaded on Mini-Protean TGX precast gels (456–1084, 4-15 %, Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... for 24 hours before being lysed in RIPA buffer containing protease inhibitors and protein concentration assessed using RcDc assay (BioRad). In some experiments cells were treated with 2μM of VX-445 (Selleckchem) ...
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bioRxiv - Molecular Biology 2021Quote: ... the obtained protein bands were transferred onto a 0.22 μm nitrocellulose membrane (MDI) using a Trans-Blot Turbo Transfer System (Bio-Rad). The membrane was blocked with 5% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant from the cell lysate was collected by centrifuging the cell lysate at 10,000 g for 10 min at 4°C and further diluted in lysis buffer to 8.0 ug/ul using a modified Bradford protein quantification assay (Bio-Rad), flash-frozen in liquid N2 ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... cell were lysed with modified RIPA buffer and EP300 was co-immunoprecipitated using magnetic Sure-bead Protein G (Bio-Rad)-coupled p300 (clone 2033 ...
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bioRxiv - Plant Biology 2020Quote: ... Denatured protein extracts were separated by SDS-PAGE on 10% TGX Stain-Free™ Fast-Cast™ gels (Bio-Rad). Separated proteins were transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell extracts were prepared using UREA lysis buffer and 30-60μg of total protein was loaded on a 7.5% or 12% denaturing polyacrylamide self-cast gel (Bio-Rad, Switzerland). Blots were incubated with the primary antibodies in TBS 0.05% Tween-20 / 5% milk overnight at 4°C and subsequently incubated with HRP coupled secondary goat anti-rabbit (7074 ...
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bioRxiv - Physiology 2020Quote: ... Equal amounts of protein were resolved on SDS-PAGE (10% Mini-PROTEAN® TGX Stain-Free™ Gels, Bio-Rad) and transferred to polyvinyl difluoride membranes (Bio-Rad) ...
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bioRxiv - Bioengineering 2020Quote: ... A pre-stained molecular weight marker was run in parallel to determine the molecular weight of the proteins (BioRad, #1610375).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... equal amounts of protein were subjected to electrophoresis on 10% SDS-PAGE gels and blotted onto PVDF membranes (Bio-Rad). Membranes were treated with antibodies using standard Western blot techniques ...
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bioRxiv - Biochemistry 2021Quote: ... 15 μg of proteins were loaded on a 10% Tris–glycine precast SDS-PAGE gel (Mini-PRO-TEAN TGX, BioRad). After a short migration (0.5 cm ...
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bioRxiv - Immunology 2021Quote: ... 1μg of each protein (wild type, single domain, and domain deletion mutant) was immobilized onto NC membranes (0.2μ pore size; Bio-Rad). The membranes were kept for drying for 5-10 min at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... The gel-separated protein bands were transferred on to a 0.2 μm nitrocellulose (Bio-Rad; Cat# 162-0112, Hercules, CA) or polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2021Quote: ... cell pellets were resuspended in 1X PBS and mechanically lysed by sonication before protein standardized by DC assay (BIO-RAD) and mixed with Western blot sample loading buffer (2X loading buffer (LI-COR) ...
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bioRxiv - Genomics 2020Quote: 20 μg of protein extracts in Laemmli buffer were run on a 4-20% gradient pre-cast gel (Bio-Rad) and transferred to nitrocellulose using Turbo transfer packs (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... The material bound on the beads was eluted in Laemmli buffer and the proteins were separated by SDS-PAGE using Criterion 4-15% acrylamide gels (BioRad) in Tris-Glycine-SDS buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were then transferred onto a PVDF membrane using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Retinas were processed and cytokines protein levels were measured by BioPlex suspension array (Bio-Rad Life Science, Hercules, CA, USA) as previously described (21).
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bioRxiv - Microbiology 2020Quote: ... Cellular lysates were separated by SDS-PAGE using 15-20 µg total cellular protein per lane (4-20% Mini-PROTEAN TGX gels (BioRad), 120 V for 1 hour) ...
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bioRxiv - Biochemistry 2021Quote: ... and the proteins containing 5× SYPRO™ Orange were loaded onto Hard-Shell® 96-well PCR Plates (Bio-Rad) for measurement ...