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Citations for Bio-Rad :
8351 - 8400 of 10000+ citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... for high-sensitivity chemiluminescent protein detection with Uvitec gel analysis systems or using enhanced chemiluminescence (ECL) reagents (Bio-Rad) with autoradiography film (Kodak Scientific Imaging Film ...
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bioRxiv - Molecular Biology 2024Quote: ... and the samples were separated on 4-20% or 4-15% Mini-PROTEAN TGX Precast Protein Gels (BioRad Laboratories). The gel was transferred to a PVDF membrane using the iBlot transfer system (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were prepared for SDS-PAGE separation by denaturation with 2x Laemmli sample buffer (Bio-Rad Laboratories; 161-0737) with 355mM β-mercaptoethanol for 5min at 95°C.
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were separated on Mini-PROTEAN® TGX™ Precast Gels (4–20% gradient, BioRad Cat. No. 4561091) in Tris-SDS-glycine buffer ...
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bioRxiv - Cell Biology 2024Quote: Anti-Flag antibody precipitated proteins in the concentrated eluate were resolved in a 4-15% Criterion TGX gel (BioRad) and stained by Colloidal Blue dye (Invitrogen) ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins in cell lysates were heated at 95[ for 5 minutes in Laemmli samples buffer (Bio-Rad, Hercules, CA), resolved by 10% SDS-PAGE ...
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bioRxiv - Immunology 2024Quote: ... and an equal amount of protein was resolved in 4-12% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) (Bio-Rad). Proteins were transferred to polyvinylidene difluoride membranes (Millipore ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were transferred to nitrocellulose membranes (GE Amersham) by semi-dry transfer for 10 min at 15V (Bio-Rad), air dried ...
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bioRxiv - Microbiology 2023Quote: Proteins from LEVs and SEVs were separated on a precast 4-15 % SDS-PAGE (Bio-Rad, Hercules, CA USA) and stained with Coomassie G-250 ...
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bioRxiv - Immunology 2024Quote: ... Total protein concentrations were measured using Pierce BCA Protein Assay Kit and equal amount of protein (10-20μg) mixed with Laemmli sample buffer were loaded in 4-20% TGX precast gels (Biorad) for gel electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of protein samples (5-20µg) were diluted in RIPA buffer and βME-Laemmli buffer (Bio-Rad Laboratories) to equal volumes and boiled for 7 minutes at 95°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were separated on an SDS-PAGE gel and transferred to a nitrocellulose membrane (BIO-RAD, Hercules, CA, USA). The membrane was blocked with EveryBlot blocking buffer (BIO-RAD ...
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bioRxiv - Cell Biology 2023Quote: ... BioRad Clarity Western ECL substrate was added to membranes to visualize protein using Bio-Rad VersaDoc Imaging System (BioRad) or iBright Imaging System (Thermofisher).
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... SDS-PAGE was followed by transfer of proteins on a nitrocellulose membrane with a semi-dry blotting system (BioRad) and protein expression was analyzed by incubation with the following primary antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Protein bands were treated with appropriate antibodies for detection and analyzed using a ChemiDoc XRS+ image analyzer (Bio-Rad). Band intensity was measured using Quantity One software (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... then separated from the protein by boiling for 10 mins at 100℃ with 4x Laemmli Sample Buffer (BioRad, #1610747).
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bioRxiv - Cell Biology 2023Quote: ... Migrated proteins were transferred from the gels to PVDF membranes using the Trans-Blot Turbo Transfer System (Bio-Rad) and run settings of 2.5A ...
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bioRxiv - Cell Biology 2023Quote: ... 1mg cell lysate or 500 µg cytosolic or nuclear fraction were incubated with 2μg antibody or normal IgG and pulled down by 5µL protein G-magnetic beads (Bio-Rad). Samples were then pulled down by protein-G-magnetic beads and subjected to WB or proteomics analysis ...
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bioRxiv - Neuroscience 2023Quote: ... were centrifuged at 20,000 x g for 25 min at 4⁰C and supernatants were assayed for total protein (Bradford assay, BioRad). Aliquots of WCL (30 µg total protein ...
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bioRxiv - Plant Biology 2023Quote: ... 20 µL samples were separated by 4-15% SDS-PAGE (Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad). A mixture of recombinant proteins was used as a standard ...
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bioRxiv - Physiology 2023Quote: ... Total protein (5∼15 µg) was loaded and separated by 4–20% pre-made SDS-PAGE gels (#4568095, BioRad, Mini-PROTEAN TGX Stain-Free Precast Gels ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins (25 μg/lane) were separated on SDS-polyacrylamide gels and transferred to polyvinylidene difluoride membranes (Bio-Rad Laboratories). Membranes were immunoblotted for CSF1R (1:250 ...
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bioRxiv - Physiology 2023Quote: ... Equal amounts of protein were fractionated by SDS-PAGE (Mini-PROTEAN TGX Precast 4– 15% gradient gels, Bio-Rad), and eletro-transferred onto nitrocellulose membrane (Trans-Blot Turbo Transfer System ...
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bioRxiv - Neuroscience 2023Quote: ... Protein content of supernatant was quantified via Bradford assay according to the manufacturer’s instructions (catalogue # 500-0006, Bio-Rad). Absorbance was measured at 595nm using a Pherastar FS (BMG LABTECH).
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bioRxiv - Neuroscience 2023Quote: ... Protein extracts were denatured 5 min at 95°C in a Laemmli buffer (Bio-Rad, Marnes-la-Coquette, France) with β-mercaptoethanol (1/10 ...
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bioRxiv - Immunology 2024Quote: ... The indicated mass of protein was boiled for 5 minutes at 95°C with 4x Laemmeli Buffer (BioRad #1610747) supplemented with 2.5% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were quantified by Pierce Reducing Agent Compatible BCA Protein Assay and run on 4-20% TGX gels (Biorad). Membranes were probed with anti-FLAG M2 antibody (Sigma ...
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bioRxiv - Cell Biology 2024Quote: Protein extracts dissolved in Lämmli sample buffer or SDS sample buffer were separated by SDS-PAGE (Biorad Mini Protean) and transferred to ethanol activated Amersham Hybond-P 0.45 µm PVDF membranes (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were resolved by 8.5% SDS-PAGE gel electrophoresis and transferred to PVDF membranes (Bio-Rad, Mississauga, ON, Canada). Membranes were blocked with 5% non-fat milk powder in Tris-buffered saline- Tween (TBS-T ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were visualized using fluorescent secondary antibodies and quantified using ChemiDoc MP device (Bio-Rad Laboratories, Hercules, CA, USA) and quantified with Image Lab software (version 6.0 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS-PAGE on a Mini-PROTEAN TGX Stain-Free pre-cast polyacrylamide gel (Bio-Rad), transferred to a hydrophobic polyvinylidene fluoride (PVDF ...
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bioRxiv - Microbiology 2023Quote: ... 10 µL of each sample was loaded onto a 4–20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and run at 180 V for 45 minutes ...
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bioRxiv - Genomics 2023Quote: ... The protein is then transferred to PVDF midi membranes using the Trans-blot Turbo Transfer System (Bio-rad, 1704150).
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bioRxiv - Neuroscience 2023Quote: ... 12% Criterion TGX precast midi protein gels (5671044) and 4x Laemmli sample buffer (1610747) were purchased from Bio-Rad Laboratories (Rishon Le Zion ...
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bioRxiv - Neuroscience 2023Quote: ... Equal protein lysate concentrations (40μg per well) were loaded on a 4%-20% precast Mini-Protean gradient TGX gel (Biorad). Proteins were separated by SDS-PAGE electrophoresis and transferred to PVDF membranes using Trans-blot turbo transfer system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein extracts were electrophoresed under reducing conditions on 4– 15% polyacrylamide gels (Bio-Rad Laboratories, Inc., Hercules, CA) and transferred onto a nitrocellulose membrane (Bio-Rad Laboratories ...
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bioRxiv - Biophysics 2023Quote: ... 100 ng of each purified protein was mixed with an appropriate volume of 2x Laemmli sample buffer (Bio-Rad) with 5 mM β-mercaptoethanol (Millipore Sigma) ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins (20 μg per sample) were separated on Mini-PROTEAN TGX 4–15% precast SDS-PAGE gels (Bio-Rad) for 1–3 hours at 100 V and then transferred to PVDF membrane (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... We loaded the lysates at equal protein amounts and concentrations on 4-15% precast TGX gels (Bio-Rad, USA) or 10% hand- poured SDS-PAGE gels ...
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bioRxiv - Developmental Biology 2023Quote: Protein was extracted from the dissected testes using RIPA buffer as described previously and quantified using Bradford reagent (Biorad). Equivalent concentration of lysate was denatured using 1X Laemelli Buffer with 1 M DTT at 95°C ...
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bioRxiv - Immunology 2023Quote: ... and membrane GAS protein fractions were separated on SDS-PAGE (Criterion TGX Gels, 4%–20% precast gels, Bio-Rad) and proteins were transferred to PVDF membranes using the trans-blot turbo transfer system (BioRad ...
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bioRxiv - Developmental Biology 2023Quote: ... and aliquots were 1:50 in water prior to Bradford protein assay (Bio-Rad, Hercules, CA, USA, Cat# 5000006). Samples were then normalized to 1 mg/mL protein in 0.1% Azo prior to reduction with 30 mM DTT at 37C for 1 h and alkylation with 30 mM chloroacetamide for 45 min ...
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bioRxiv - Plant Biology 2023Quote: ... Samples containing 6 µg total protein were resolved using pre-cast SDS-PAGE Any KD™ gels (BIO-RAD), transferred to a polyvinylidene difluoride membrane (Immobilon-FL 0.45 µm ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred to 0.2 μm Nitrocellulose membranes using semi-dry transfer (Bio-Rad Transblot Turbo; 25V 30 minutes). Membranes were blocked using 5% milk in Tris-buffered saline supplemented with 0.1% Tween-20 (TBST ...
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bioRxiv - Immunology 2022Quote: ... Lysates from 3.107 to 4.107 of cells were subjected to immunoprecipitation according to manufacturer’s protocol for the Sure Beads Protein G Magnetic Beads (161-4023, Biorad) using a rat anti-FLAG antibody (Biolegend) ...
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bioRxiv - Microbiology 2022Quote: ... and 10-15 µg of protein was loaded into 12% acrylamide Criterion XT Bis-Tris Precast Gels (Bio-Rad). Electrophoresis was performed with NuPage MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... and protein levels were quantified using BCA Assay (Pierce) and supplemented with 4x Laemmli Buffer with 2-mercaptoethanol (Biorad). Samples were boiled and sonicated ...
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bioRxiv - Cancer Biology 2022Quote: ... The resolved proteins were transferred onto a polyvinylidene difluoride membrane using the Trans-Blot Turbo Transfer System (Bio-Rad). The membranes were stained using 5% Ponceau S solution ...
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bioRxiv - Biochemistry 2022Quote: ... and protein purity was determined by SDS-PAGE analysis (Figure S8) and densitometry on Image Lab 6.0 (Bio-Rad).
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bioRxiv - Cancer Biology 2022Quote: ... 50µg of protein were loaded and electrophoresed through a 4-15% Mini-PROTEAN TGX precast gel (BIO-RAD, 4561084). Proteins were transferred onto a PVDF membrane ...