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Citations for Bio-Rad :
8151 - 8200 of 10000+ citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... the membranes were washed several times and proteins were visualized using the Clarity™ Western ECL Substrate (Bio-Rad) and the ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Biochemistry 2021Quote: ... 5 μM of proteins and 3x of SYPRO™ Orange were pipetted into a 96-well PCR plate (BioRad, MicroAmp Fast 96-Well Reaction plate ...
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bioRxiv - Biochemistry 2021Quote: ... The enriched protein were eluted by heating at 95°C for 30 minutes in 1X Laemmli sample buffer (BioRad) and processed to western blot ...
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bioRxiv - Synthetic Biology 2021Quote: ... following the manufacturer’s instruction (4–15% Mini-PROTEAN® TGX Stain-Free™ Protein Gels, 4568084, BioRad, Hercules, CA) with the gel apparatus (Mini-PROTEAN Tetra Cell ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoblotting was performed using standard protocols with 10μg of protein loaded onto SDS-PAGE pre-cast gels (Bio-Rad). Antibodies used were anti-DDX3X (rabbit polyclonal ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein were loaded onto 10% or 12% pre-cast Tris-HCl Mini-PROTEAN gels (Bio-Rad) and proteins separated by electrophoresis ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was then transferred into a new tube and protein concentration was determined by BCA assay (Bio-Rad). Immunoblotting was performed using CDCP1(D1W9N ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by SDS-PAGE in 12% Bis-Tris gels and transferred onto 0.45 µm nitrocellulose membranes (BioRad). The blots were incubated overnight at 4°C with anti-αSyn (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were separated by SDS-PAGE and transferred to nitrocellulose using a Trans-blot Turbo system (Bio-Rad Laboratories). Species-specific secondary antibodies conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Genomics 2020Quote: ... The proteins were transferred onto PVDF membranes using the Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad, 170-3930) at 80 V ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were subsequently aliquoted by mixing 75μl of protein extract with 25μl of 4X Laemmli buffer (Bio-Rad #1610747) and stored at -20°C ...
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bioRxiv - Microbiology 2020Quote: ... 25 µg of protein was run per sample on 4-15% Mini-PROTEAN TGX stain-free precast gels (BioRad) at 150V ...
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bioRxiv - Immunology 2021Quote: ... Protein extracts were separated by SDS-PAGE with a 10% gel and transferred to nitrocellulose membranes (Bio-Rad Laboratories). The membranes were probed with anti-spike Ab (40150-T62 ...
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bioRxiv - Immunology 2021Quote: ... 40ug of protein was loaded per well for polyacrylamide gel electrophoresis using Mini-PROTEAN Precast Polyacrylamide Gels (Bio-Rad). Western blotting was performed using low fluorescence PVDF membrane (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... For the visualization the proteins ECL-solution (Luminol, Para-Hydroxycoumarinacid, DMSO) and ChemiDoc Touch™ Imaging System (BIO-RAD) was used ...
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bioRxiv - Immunology 2020Quote: ... Protein for immunoblotting was eluted from 25 percent of beads by boiling in 2X Laemmli sample buffer (Bio-Rad). RNA was extracted from 75 percent of beads using TRIzol reagent (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Soluble proteins were separated using SDS-PAGE and transferred to a 0.45 µm nitrocellulose membrane (Cat# 1620115, Bio-Rad). Primary antibodies (Table 2 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins from a second identically loaded gel were transferred to PVDF membrane using a semi-dry transfer method (BIORAD) and a western blot was performed using anti-HIS (Sigma H-1029 ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 20ug of protein per sample was resolved using 4%-15% TGX stain-free gels (Bio-Rad), followed by immunoblotting using PCF11 (Proteintech ...
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bioRxiv - Neuroscience 2020Quote: ... Lysates were cleared of debris by centrifugation at 15,000 x g for 15 min at 4°C and then normalized for protein content using Bradford assay (BioRad). Normalized protein lysates were fractionated by 10 % SDS/PAGE and transferred onto a PVDF membrane (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred to 0.2 μm Nitrocellulose membranes using semi-dry transfer (Bio-Rad Transblot Turbo; 25V 30 minutes). Membranes were blocked using 5% milk in Tris-buffered saline supplemented with 0.1% Tween-20 (TBST ...
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bioRxiv - Immunology 2022Quote: ... Lysates from 3.107 to 4.107 of cells were subjected to immunoprecipitation according to manufacturer’s protocol for the Sure Beads Protein G Magnetic Beads (161-4023, Biorad) using a rat anti-FLAG antibody (Biolegend) ...
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bioRxiv - Microbiology 2022Quote: ... and 10-15 µg of protein was loaded into 12% acrylamide Criterion XT Bis-Tris Precast Gels (Bio-Rad). Electrophoresis was performed with NuPage MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... and protein levels were quantified using BCA Assay (Pierce) and supplemented with 4x Laemmli Buffer with 2-mercaptoethanol (Biorad). Samples were boiled and sonicated ...
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bioRxiv - Cancer Biology 2022Quote: ... The resolved proteins were transferred onto a polyvinylidene difluoride membrane using the Trans-Blot Turbo Transfer System (Bio-Rad). The membranes were stained using 5% Ponceau S solution ...
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bioRxiv - Biochemistry 2022Quote: ... and protein purity was determined by SDS-PAGE analysis (Figure S8) and densitometry on Image Lab 6.0 (Bio-Rad).
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bioRxiv - Cancer Biology 2022Quote: ... 50µg of protein were loaded and electrophoresed through a 4-15% Mini-PROTEAN TGX precast gel (BIO-RAD, 4561084). Proteins were transferred onto a PVDF membrane ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... proteins were separated on a 4-15% Mini-PROTEAN TGX Stain-Free SDS-PAGE gel (#456-8084; Bio-Rad) and transferred to a 0.2 μm PVDF membrane (#ISEQ00010 ...
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bioRxiv - Immunology 2022Quote: ... Proteins were separated on a 4-15% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) precast gel (Bio-Rad) at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... 4 °C) and the supernatant was taken up and the protein concentration was determined using the Bradford assay (Biorad).
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bioRxiv - Physiology 2022Quote: ... S-nitrosylated proteins were separated using 10% SDS-PAGE and transferred onto a PVDF membrane (BioRad, Hercules, CA, USA). A monoclonal anti-Cx43 antibody (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentrations of culture medium and cell lysate were determined by the Bradford assay (Bio-Rad, Hercules, CA). Culture medium or cell lysate samples were resuspended in SDS sample buffer containing β-mercaptoethanol and incubated at 95°C for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 µg of protein extract were loaded onto a Mini-PROTEAN TGX Precast Gel (4-15% gradient, BioRad, 4561086) in 1xLaemmli buffer (BioRad ...
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bioRxiv - Neuroscience 2022Quote: ... Samples (50µg of protein/lane) were separated on a 4-12% SDS-polyacrylamide gel (Bio-Rad, Hercules, CA, USA) and probed with anti-ACK antibody (1:100 ...
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bioRxiv - Microbiology 2022Quote: ... total protein was visualised post-transfer to PVDF membranes on ChemiDoc using Stain-free fluorescent dye (Bio-Rad Laboratories). For analyses of protein band intensities ...
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bioRxiv - Immunology 2022Quote: ... loaded onto a 12% acrylamide gel and separated proteins transferred onto a PVDF membrane (Bio-Rad, Hercules, California, US). Membranes were washed in 1X TBS ...
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bioRxiv - Microbiology 2022Quote: ... the denaturalized proteins were separated in a 12% SDS-PAGE precast gel (Mini-PROTEAN TGX Precast Gels, Bio-Rad). Electrophoresis was run at 250 V (PowerPac Basic power supply ...
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bioRxiv - Plant Biology 2022Quote: ... 20 µl of protein extract was mixed with 180 µl of five-time diluted Bradford reagent (Bio-Rad, US) in a 96-well microplate ...
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bioRxiv - Microbiology 2022Quote: 25 μl of the secreted proteins were ran on a Mini-PROTEAN® TGX™ precast gel (Bio-Rad). The whole lane of the gel for each sample was sliced into 4 mm sections and washed with 1 ml of 50% ethanol in 50 mM ammonium bicarbonate (ABC) ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were then electroblotted onto a PVDF membrane using a semidry blotter (Trans-Blot Turbo Transfer System; Bio-Rad). Membranes were blocked overnight at 4 °C in TBS-T (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2022Quote: The input or eluted proteins were separated by SDS-polyacrylamide-gel electrophoresis (PAGE) gels using Gel Electrophoresis (Bio-Rad), and blotted onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Physiology 2023Quote: ... and isolated protein complexes were eluted from the beads in 1X SDS sample buffer (Bio-Rad Laboratories, Hercules, CA) at 60°C for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: Two thirds of eluted protein sample from HA Immunoprecipitation were run on a 4-15% Mini-PROTEAN (Bio-Rad) gel to separate proteins and remove synthetic HA peptide ...
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bioRxiv - Physiology 2022Quote: ... proteins were separated by running the gel in an electrophoresis chamber (Mini-PROTEAN Tetra Vertical Electrophoresis Cell, Bio-Rad) at 200 V for 30 min ...
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bioRxiv - Physiology 2024Quote: ... 6 μg of protein from each sample was loaded into a 4-20% Mini-PROTEAN® TGX gel (BioRad) and proteins were separated by size using electrophoresis ...
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... for high-sensitivity chemiluminescent protein detection with Uvitec gel analysis systems or using enhanced chemiluminescence (ECL) reagents (Bio-Rad) with autoradiography film (Kodak Scientific Imaging Film ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were quantified by Pierce Reducing Agent Compatible BCA Protein Assay and run on 4-20% TGX gels (Biorad). Membranes were probed with anti-FLAG M2 antibody (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were resolved by 8.5% SDS-PAGE gel electrophoresis and transferred to PVDF membranes (Bio-Rad, Mississauga, ON, Canada). Membranes were blocked with 5% non-fat milk powder in Tris-buffered saline- Tween (TBS-T ...
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bioRxiv - Cell Biology 2024Quote: Protein extracts dissolved in Lämmli sample buffer or SDS sample buffer were separated by SDS-PAGE (Biorad Mini Protean) and transferred to ethanol activated Amersham Hybond-P 0.45 µm PVDF membranes (Cytiva ...
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bioRxiv - Immunology 2024Quote: ... The indicated mass of protein was boiled for 5 minutes at 95°C with 4x Laemmeli Buffer (BioRad #1610747) supplemented with 2.5% (v/v ...