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Citations for Bio-Rad :
8451 - 8500 of 10000+ citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 25 μg of total protein extracts were run on a gradient (15-4%) Polyacrylamide gel (MINIi-PROTEAN TGX Precast Gels, Biorad). Proteins were transferred on nitro-cellulose membrane and checked with Red Ponceau staining for efficient transfer ...
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bioRxiv - Molecular Biology 2021Quote: ... Denatured protein samples were then resolved in a 10% SDS-PAGE and transferred to a PVDF membrane (Bio-Rad, #1620177). Blots were probed by custom-designed Drosophila antibodies as previously described 6 diluted in PBS-0.1% Tween supplemented with 5% nonfat milk ...
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bioRxiv - Pathology 2022Quote: ... Appropriate total proteins from different lines were resolved on 10 % polyacrylamide gels and transferred to a nitrocellulose membrane (Bio-Rad). Anti-FLAG monoclonal antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... and equal amount of proteins were separated by SDS/PAGE on pre-cast 4-20% polyacrylamide gradient gels (Cat# 5671094, Biorad) and transferred to immune-blot PVDF membrane (Merck cat# IPVH00010) ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were resolved by gel electrophoresis on a 4 to 20% polyacrylamide gradient Mini-Protean TGX precast gel (Bio-Rad) and transferred to an Immobilon-P membrane (Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were separated at 175 V for 3-4 hours at 4°C and then transferred to nitrocellulose membrane (BioRad) by using a Criterion blot cell (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoreactivities of the blotted proteins were detected with Clarity Western ECL Substrate for Chemiluminescent Western Blot Detection (Bio-rad, U.S.A.) and ChemiDoc XRS+ system (Bio-rad ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were heated at 60°C and loaded on Mini-Protean TGX precast gels (456–1084, 4-15 %, Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... for 24 hours before being lysed in RIPA buffer containing protease inhibitors and protein concentration assessed using RcDc assay (BioRad). In some experiments cells were treated with 2μM of VX-445 (Selleckchem) ...
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bioRxiv - Molecular Biology 2021Quote: ... the obtained protein bands were transferred onto a 0.22 μm nitrocellulose membrane (MDI) using a Trans-Blot Turbo Transfer System (Bio-Rad). The membrane was blocked with 5% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant from the cell lysate was collected by centrifuging the cell lysate at 10,000 g for 10 min at 4°C and further diluted in lysis buffer to 8.0 ug/ul using a modified Bradford protein quantification assay (Bio-Rad), flash-frozen in liquid N2 ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... cell were lysed with modified RIPA buffer and EP300 was co-immunoprecipitated using magnetic Sure-bead Protein G (Bio-Rad)-coupled p300 (clone 2033 ...
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bioRxiv - Cell Biology 2019Quote: ... Polyacrylamide gels used in this study were either prepared using 10% EZ-Run protein gel solution (Fisher) or precast gradient gels (4-20%, from Biorad). Any quantification performed on western blots was done using ImageJ software ...
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bioRxiv - Microbiology 2019Quote: ... YpdA and YpdAC14A proteins were pre-reduced with 10 mM DTT followed by DTT removal with Micro Biospin 6 columns (Biorad). For the biochemical activity assays of the specific BSSB reductase activity ...
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bioRxiv - Microbiology 2019Quote: ... Protein concentrations of the DMSO control samples were measured in triplicate using a detergent compatible (DC) assay (Bio-Rad, USA). Then ...
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bioRxiv - Cell Biology 2019Quote: ... and 25-30 μg of protein were loaded on a 7.5% separating gel using Mini Trans-Blot Cell (Bio-Rad). Nitrocellulose membranes were stained with primary antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... The target proteins were detected with the Clarity™ Western ECL Blotting Substrate (#170-5061, Bio-Rad, Hercules, CA, USA). The sizes of proteins were estimated with a PageRuler™ Prestained Protein Ladder (10-180 kDa ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples (10 µL) were run on 12.5% SDS-PAGE gels along with a Precision Plus Protein dual colour standard (Bio-Rad) and transferred to PVDF membranes ...
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bioRxiv - Plant Biology 2020Quote: ... Denatured protein extracts were separated by SDS-PAGE on 10% TGX Stain-Free™ Fast-Cast™ gels (Bio-Rad). Separated proteins were transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Plant Biology 2019Quote: ... The exchanged sample was diluted to either 0.8 or 0.4mg/ml via the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, CA). Samples were divided into 100µL aliquots and flash frozen with liquid nitrogen ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell extracts were prepared using UREA lysis buffer and 30-60μg of total protein was loaded on a 7.5% or 12% denaturing polyacrylamide self-cast gel (Bio-Rad, Switzerland). Blots were incubated with the primary antibodies in TBS 0.05% Tween-20 / 5% milk overnight at 4°C and subsequently incubated with HRP coupled secondary goat anti-rabbit (7074 ...
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bioRxiv - Molecular Biology 2019Quote: ... protein samples were separated by SDS-PAGE and electrophoretically transferred to a polyvinylidene fluoride membrane (Bio-Rad Laboratories, Hercules, CA). After 1-h blocking ...
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bioRxiv - Physiology 2020Quote: ... Equal amounts of protein were resolved on SDS-PAGE (10% Mini-PROTEAN® TGX Stain-Free™ Gels, Bio-Rad) and transferred to polyvinyl difluoride membranes (Bio-Rad) ...
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bioRxiv - Bioengineering 2019Quote: ... Lysate samples were analysed for recombinant protein expression by SDS PAGE (12% Mini-PROTEAN-TGX stain-free gel; Bio-Rad), using Precision Plus unstained protein ladder (Bio-Rad ...
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bioRxiv - Biochemistry 2019Quote: ... The spin-labeled protein was further concentrated and free label was removed using Micro BioSpin 6 Chromatography Columns (Bio-rad) pre-equilibrated with buffer C.
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bioRxiv - Systems Biology 2019Quote: ... Equal amounts of protein were loaded onto the acrylamide gel (Bio-Rad Mini or Midi PROTEAN® TGX precast gels). For the transfer of proteins to the PVDF membrane ...
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bioRxiv - Cell Biology 2019Quote: Isolated dermal endothelial cells were lysed in Laemmli sample buffer and proteins were separated on a 7.5% Mini-Protean TGX precast gel (#456-1025, Bio-rad) using Tris/glycine/SDS running buffer (#161-0732 ...
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bioRxiv - Immunology 2019Quote: ... and 500 ng protein was loaded to 10% TGX (Tris-Glycine eXtended) Stain-Free FastCast polyacrylamide gels (Bio-rad, # 1610183), 200 V running for 45min ...
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bioRxiv - Bioengineering 2020Quote: ... A pre-stained molecular weight marker was run in parallel to determine the molecular weight of the proteins (BioRad, #1610375).
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bioRxiv - Microbiology 2019Quote: ... Protein lysates were separated by SDS-PAGE and western blot analysis was performed using the ChemiDoc XRS+ imaging system (BioRad). Western blots were analyzed using Image Lab version 5.2.1 software (BioRad ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... equal amounts of protein were subjected to electrophoresis on 10% SDS-PAGE gels and blotted onto PVDF membranes (Bio-Rad). Membranes were treated with antibodies using standard Western blot techniques ...
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bioRxiv - Biochemistry 2021Quote: ... 15 μg of proteins were loaded on a 10% Tris–glycine precast SDS-PAGE gel (Mini-PRO-TEAN TGX, BioRad). After a short migration (0.5 cm ...
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bioRxiv - Immunology 2021Quote: ... 1μg of each protein (wild type, single domain, and domain deletion mutant) was immobilized onto NC membranes (0.2μ pore size; Bio-Rad). The membranes were kept for drying for 5-10 min at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... The gel-separated protein bands were transferred on to a 0.2 μm nitrocellulose (Bio-Rad; Cat# 162-0112, Hercules, CA) or polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2021Quote: ... cell pellets were resuspended in 1X PBS and mechanically lysed by sonication before protein standardized by DC assay (BIO-RAD) and mixed with Western blot sample loading buffer (2X loading buffer (LI-COR) ...
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bioRxiv - Genomics 2020Quote: 20 μg of protein extracts in Laemmli buffer were run on a 4-20% gradient pre-cast gel (Bio-Rad) and transferred to nitrocellulose using Turbo transfer packs (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... The material bound on the beads was eluted in Laemmli buffer and the proteins were separated by SDS-PAGE using Criterion 4-15% acrylamide gels (BioRad) in Tris-Glycine-SDS buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were then transferred onto a PVDF membrane using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Retinas were processed and cytokines protein levels were measured by BioPlex suspension array (Bio-Rad Life Science, Hercules, CA, USA) as previously described (21).
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bioRxiv - Microbiology 2020Quote: ... Cellular lysates were separated by SDS-PAGE using 15-20 µg total cellular protein per lane (4-20% Mini-PROTEAN TGX gels (BioRad), 120 V for 1 hour) ...
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bioRxiv - Biochemistry 2021Quote: ... and the proteins containing 5× SYPRO™ Orange were loaded onto Hard-Shell® 96-well PCR Plates (Bio-Rad) for measurement ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were separated by SDS-PAGE using a Mini-Protein Tera Cell system (Bio-Rad Laboratories, Inc., Hercules, USA, CA). Proteins were visualized by CBB staining as described elsewhere.
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bioRxiv - Biochemistry 2021Quote: ... respectively 45 μL and 10 μL of proteins were loaded for each condition and separated on gradient 4-12% polyacrylamide TGX Stain-Free pre-cast gels (Biorad) and transferred onto nitrocellulose membrane (Biorad) ...
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bioRxiv - Biochemistry 2020Quote: ... The extracted protein (1000 mg) was loaded onto the 17 cm IPG gel strips (nonlinear, pH 3.0-10.0, Bio-Rad, USA) according to Chen et al (14) ...
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bioRxiv - Biochemistry 2020Quote: ... Tissue lysates for Western blot were prepared as previously described [11] and sample protein content was determined by Bradford assay (BioRad). Lysates were loaded in 10% polyacrylamide gels and transferred to Immobilon membranes (Millipore ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared of insoluble debris by centrifugation prior to determination of protein concentration by BCA assay (BioRad, CA, USA). Lysates were electrophoresed through either 4-12% NuPAGE or 8% E-PAGE gels (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... Around 40 µg of proteins were loaded per lane of a 4-12% precast gel (Bio-Rad Mini-PROTEAN®). Proteins were transferred on PVDF-membrane and blocked for 1h with 5% skimmed milk ...
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bioRxiv - Biochemistry 2020Quote: ... Gels were either imaged directly (for fluorescent proteins) or stained with Coomassie blue and then imaged using a ChemiDoc gel imager (BioRad). Gel images were analyzed using Fiji ...
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bioRxiv - Biochemistry 2020Quote: ... The concentration was then measured by DC assay and 20 μg of protein was resolved on an SDS page gel (gradient 4%-20%, BioRad) and transferred to a nitrocellulose membrane ...
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bioRxiv - Neuroscience 2020Quote: ... Protein was transferred to a nitrocellulose membrane (Hybond ECL; Amersham Biosciences) using a semi-dry transfer system (Bio-Rad Laboratories) at 25mV for 45 minutes ...