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Citations for Bio-Rad :
8301 - 8350 of 10000+ citations for Human Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Thirty micrograms of denatured cell lysate proteins were loaded for SDS-PAGE electrophoresis (4–20% TGX gel, Bio-Rad). Primary antibodies include ALDH3B2 (Proteintech ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were separated by SDS–PAGE electrophoresis using 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad) followed by detection of the ubiquitinated substrate by immunoblotting using anti-MBP (New England Biolabs) ...
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bioRxiv - Neuroscience 2024Quote: ... The protein lysates were then loaded onto an 10% Ready Gel® Tris- HCl Precast Gels (BioRad, catalog#1611119) in running Buffer (BioRad ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... The same amount of protein was loaded in each lane of Mini-PROTEAN TGX gels (4–20%, Bio-Rad). The antibody signals were detected by chemiluminescence using ImmunoStar LD reagent (290-69904 ...
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bioRxiv - Cell Biology 2023Quote: ... 20-40 ug of protein was loaded per lane in a pre-cast gel for SDS-PAGE (Bio-Rad). Gel was then transferred using the TurboBlot Transfer (Bio-Rad ...
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bioRxiv - Synthetic Biology 2024Quote: ... Samples prepared with equal amounts of proteins were run on 10% Mini-PROTEAN TGX Precast Gels (Bio-Rad, 4561033) (for ASGR1 blot ...
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bioRxiv - Microbiology 2024Quote: ... and total protein was wet transferred to a nitrocellulose membrane using a mini Trans-Blot electrophoretic system (Bio-Rad). After the transfer ...
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bioRxiv - Immunology 2024Quote: ... The SEC-eluted protein peaks corresponding to the correct retention volume based on the molecular weight standards (BioRad: 1511901) were collected and concentrated using Amicon Ultra centrifugal filters (Millipore Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Protein samples were loaded to a 48-well Bio-Dot® microfiltration apparatus (Bio-Rad Laboratories, Hercules, CA, USA) with BioTraceTM NT nitrocellulose membrane (Pall Life Sciences ...
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bioRxiv - Biophysics 2024Quote: ... Equal volumes of protein samples were resolved on 4-12% gradient SDS-polyacrylamide gel and transferred onto 0.2μM nitrocellulose (BioRad #1620112) or PVDF (BioRad #1620177 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated on a 12% SDS-PAGE gel and transferred to PVDF membranes by wet electroblotting (Bio-Rad). To improve signal detection ...
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bioRxiv - Plant Biology 2024Quote: ... The proteins were separated on a 4 to 20% Trist-glycine stain free polyacrylamide 10-well gel (Bio-Rad) at 170 V for approximately 1 h in 1x Tris/glycine/SDS running buffer ...
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bioRxiv - Microbiology 2024Quote: Protein fractions were analyzed by standard denaturing SDS-PAGE electrophoresis using 4 to 20% gradient mini-gels (Bio-Rad), InstantBlue Coomassie protein stain ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of proteins were resolved by polyacrylamide-SDS gel electrophoresis (PAGE) and transferred to nitrocellulose membrane (Bio-Rad) using Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were transferred onto a polyvinyldiene fluoride (PVDF) membrane in a Mini Trans-Blot cell (Bio-Rad, CA, USA) at a constant current of 315 mA on ice for 90 min ...
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bioRxiv - Biochemistry 2024Quote: ... separated by SDS-PAGE on a pre-cast 4–15% Criterion™ Protein Gel (Bio-Rad Laboratories, Cat#5671084), and the gel was stained using QC Colloidal Coomassie Stain (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... and at 100-120 V through the resolving gel until the protein xtra prestained ladder (Bio-Rad catalog # 1610377) achieved good separation ...
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bioRxiv - Plant Biology 2024Quote: ... Protein was transferred to 0.45 µm nitrocellulose membrane (Cytiva Amersham Protran #10600003) using a tank system (Biorad #1703930, #1704070) in transfer buffer (25 mM Tris ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were transferred to PVDF membranes with NuPAGE Transfer Buffer in a Trans-Blot Turbo transfer apparatus (Bio-Rad) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were transferred onto PVDF membranes using the Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad, 170-3930) at 80 V ...
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bioRxiv - Cell Biology 2024Quote: ... Protein was then separated by size-exclusion chromatography (HiLoad 16/600 Superdex 200 pg, Cytiva) via FPLC (Bio-Rad). Purified protein was stored in Gel Filtration Buffer + 25% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... Ribosome pellet was resuspended in same lysis buffer and protein concentration was determined via Bradford Assay (Bio-Rad, #5000205) prior to western blot.
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bioRxiv - Microbiology 2024Quote: Proteins were transferred to a nitrocellulose membrane using the Trans-Blot Turbo Transfer System (Bio-Rad, Hercules, CA, USA) and then blocked with 5% (w/v ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... were used to reveal proteins transferred on nitrocellulose membrane with the Trans-Blot Turbo Transfer System (Bio-Rad, 1704150). Membranes were blocked in PBS 0.1% Tween20 (PBST ...
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bioRxiv - Molecular Biology 2024Quote: ... The separated proteins were electrotransferred using a Trans-Blot® Turbo™ Mini PVDF Transfer Pack and System (Biorad). After blocking for 1 h in 0.5% Tween-PBS in 5% skim milk (blocking solution) ...
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bioRxiv - Microbiology 2024Quote: ... the proteins were transferred to PVDF membranes using Trans-Blot Turbo PVDF Transfer Packs (Bio-Rad, Hercules, CA, USA), and the membranes were blocked with Blocking One (Nacalai Tesque) ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate were separated on a 4–20% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (BIO-RAD), transferred to polyvinylidene difluoride membranes (0.2 um ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... from ART or ART + GYY4137 treated groups were harvested to isolate total RNA using Qiagen RNAeasy isolation kit and 200 ng of total RNA was reverse transcribed (iScript™cDNA synthesis kit, Bio-Rad). Reverse transcribed cDNA was diluted 10-fold and amplified using primers against HIV LTRs and seminested – PCR was performed using primers and probe listed in Table S6 ...
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bioRxiv - Developmental Biology 2021Quote: ... using the whole SingleShot™ Cell Lysis Kit (SingleShot™ Cell Lysis Kit, 100 x 50 µl reactions Cat# 1725080, Bio-Rad) as follows ...
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bioRxiv - Physiology 2020Quote: ... RNA of treated cells was isolated using EZ-RNA isolation kit (Biological Industries Beit Haemek, Israel) and cDNA was synthesised using iScript cDNA synthesis kit (Bio-Rad, UK). Real-time qPCR analyses were performed on a StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genetics 2021Quote: ... we purified total RNA using the Qiagen RNeasy Mini kit (Cat#74106) and made cDNA using iscript cDNA synthesis kit from Biorad (Cat#1725036), followed by a Taqman assay to detect AHR transcription (Hs00169233-m1 for AHR expression and Hs0442063-g1 for GAPDH ...
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bioRxiv - Biochemistry 2021Quote: RT-qPCR was performed using the 2019-nCoV RUO Kit (Integrated DNA Technologies, #10006713) in combination with the iTaq™ Universal SYBR® Green One-Step Kit (BioRad). Each 20 μl reaction contained 10 μl 2X iTaq universal SYBR® Green reaction mix ...
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bioRxiv - Evolutionary Biology 2021Quote: RNA was isolated from cells using the Qiagen RNeasy Mini Kit (Cat# 74104) and cDNA was prepared using the iScript cDNA Synthesis Kit (Biorad, Cat# 1708890). Assays were done in triplicate using Express SYBR GreenER master mix (Invitrogen ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... Whole-larvae cDNA was generated by isolating total RNA from 3–5 dpf zebrafish larvae using the E.Z.N.A Total RNA kit (Omega, Bio-Tek INC, Cat# R6834-01) and reverse transcribing using the iScript cDNA synthesis kit (BioRad, Cat# 1708891). Fragments of coding sequences of these genes were amplified by PCR using gene-specific primers as follows:
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bioRxiv - Bioengineering 2024Quote: ... RNA was extracted using a total RNA purification kit (NORGEN, Auburn, WA) followed by cDNA synthesis utilizing iScript cDNA kit (BIORAD, Hercules, CA). Quantitative real time-PCR using iTaq Universal SYBR GREEN Supermix (BIORAD ...
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bioRxiv - Cancer Biology 2021Quote: ... a DNA extraction kit (InstaGene Matrix-Bio-Rad – USA) is utilize zed for this purpose ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA was synthesized using iScript cDNA Synthesis Kit (BioRad). qPCR was performed on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... before reverse transcription using iScript cDNA Synthesis kit (BioRad). Real-time PCR was performed with diluted cDNAs in a 15 μL reaction volume using Kapa SYBR Fast qPCR mix in duplicates and analyzed using the CFX96 Touch Real-Time PCR Detection System and computing ΔΔCq using the CFX96 software ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were developed with an ECL reagent kit (BioRad). Western blots were quantified using Fiji software (“Gels” command).
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bioRxiv - Genomics 2021Quote: ... whereas a KRAS mutation screening kit (Biorad, Hercules, CA) was applied to patient M10 ...
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bioRxiv - Developmental Biology 2020Quote: ... or with the iScript cDNA Synthesis Kit (Bio-Rad). Real-time PCR was performed using SYBR Green (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... The iScript Reverse Transcription Supermix kit (Bio-Rad; 1708841) was used to synthesize cDNAs from 200 ng of RNA according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: The iTaq Universal Probes One-Step kit (Bio-Rad) was used to perform duplex assays probing for the HCV genome (NS5B-FW primer ...
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bioRxiv - Neuroscience 2022Quote: ... or the iScript cDNA Synthesis Kit (Bio-Rad, 1708891). RT-qPCR was performed using the SensiFAST Probe No-ROX Kit (Bioline ...
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bioRxiv - Biochemistry 2022Quote: ... detection was performed using reagents from Biorad (Clarity Western ECL kit) and revealed using ChemiDocMPSystem (BioRad) or the Fusion FX (Vilber ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized using an iScript kit (Bio-Rad). qRT-PCR was performed by using Sso-Advanced Universal SYBR Green Supermix Kit (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... The iScript cDNA synthesis kit (Bio-Rad Laboratories, Inc.) was used for cDNA synthesis ...
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bioRxiv - Developmental Biology 2020Quote: ... Immuno-Star Western C Kit (BioRad catalog 170-5070) was used ...
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bioRxiv - Immunology 2019Quote: ... cDNA was made with iScript Direct Synthesis kits (BioRad) per manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... Bands were visualized using an ECL kit (Bio-Rad). α-Tubulin and Lamin A/C served as a loading control ...