Labshake search
Citations for Bio-Rad :
7401 - 7450 of 7510 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... BPH-1 cell viability was assessed using Trypan Blue staining quantified on a Biorad TC 20 (Bio-Rad, Marnes-la-Coquette, France) after 4 days of treatment with appropriate drugs.
-
bioRxiv - Cell Biology 2024Quote: ... Gel was transferred at 350 Amperes for 120 minutes onto pre-activated polyvinylidene difluoride (PVDF) or Nitrocellulose membranes in a Mini-Protean® 3 Cell (BioRad Laboratories). Correct protein transfer was checked by red Ponceau S Solution (Panreac) ...
-
bioRxiv - Cell Biology 2024Quote: ... having diameter of 200nm were ballistically shot inside the cell using a gene gun system (Bio-Rad, Helios® Gene gun system). After the shooting ...
-
bioRxiv - Plant Biology 2020Quote: Temperature sensitive mutant cells were transformed with the linearized plasmid by electroporation (500 V, 50 μF, 800□Ω) using GenePulser Xcell (Bio-Rad, CA). The cells were recovered in TAP containing 40□mM sucrose ...
-
bioRxiv - Biophysics 2021Quote: ... and transfected with Fluorescence Multiplex-Host Cell Reactivation (FM-HCR) reporter plasmids using the Gene Pulser MXCell Plate Electroporation System (Bio-Rad, #165-2670) using conditions 260 V ...
-
bioRxiv - Cell Biology 2020Quote: ... 40 μg of whole cell protein lysate were applied to 4%-15% Mini-PROTEAN TGX precast protein Gel (Bio-Rad, Cat No. 4561084) with electrophoresis and then transferred to a nitrocellulose membrane ...
-
bioRxiv - Cell Biology 2020Quote: ... Supernatants were collected and stored at −80°C and cells were lysed in 1x Laemmli Buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA) for further analysis.
-
bioRxiv - Biochemistry 2021Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
-
bioRxiv - Biochemistry 2020Quote: Cell or tissue protein lysates were separated by SDS-PAGE on pre-cast 8-16% polyacrylamide gels and transferred onto nitrocellulose membranes using a Trans-Blot® Semi-Dry transfer cell (all purchased from Bio-Rad Laboratories), blocked in non-fat milk and incubated overnight at 4°C with primary antibody for HILPDA (1:750 ...
-
bioRxiv - Molecular Biology 2020Quote: ... The lysates were centrifuged at 12,000g for 20 mins to remove cell debris and protein concentration in supernatants was determined using DC™ Protein Assay (Bio-Rad, #5000111). Twenty μg of proteins from L ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were recovered from the resulting BAL fluid by centrifugation (500 g, 20 min, 4°C) and counted using an automated cell counter (TC20; Bio-Rad, Hercules, CA). All animal care procedures and experiments were approved by the University of Arizona Animal Care and Use Committee (Approval #13-490).
-
bioRxiv - Plant Biology 2020Quote: Proteins were transferred from an SDS-polyacrylamide gel to a polyvinylidene difluoride (PVDF) membrane by electroblotting using a Trans-Blot SD transfer cell (Bio-Rad, www.bio-rad.com) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
-
bioRxiv - Cell Biology 2021Quote: ... Similar amount of total cell lysate were fractionated through a 10% SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad Lab, California, USA). The proteins transferred to the membrane were visualized by Ponceau solution stain 0.3% for 5 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... The proteins were then transferred to a polyvinylidene difluoride (PVDF) membrane applying 20 mA for 20 minutes in a semi-dry transfer cell (Bio-Rad Laboratories, USA). The membrane was blocked in 0.5% powdered milk in TBS-T buffer at 4°C overnight ...
-
bioRxiv - Biochemistry 2022Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
-
bioRxiv - Neuroscience 2021Quote: Protein lysates from SH-SY5Y cells were quantified (bicinchoninic acid protein assay, Pierce) and then electrophoresed in 4—12% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad) in 1X TGS Tris/Glycine/SDS Buffer (10X TGS Buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... were co-electroporated with 1.5 x 107 KH2 ES cells at 400 V and 125 μf using two pulses in a Gene PulserII (Bio-Rad, Hercules, CA). Recombination between FRT sites such that the entire plasmid is inserted within the PGK promoter and the ATG initiation codon upstream and in frame with the hygromycin resistance gene was used to select correctly targeted cells ...
-
bioRxiv - Bioengineering 2021Quote: ... All plasmids in this study have been transformed into cells using electroporation with the Bio-Rad Micropulser™ (Bio-Rad Laboratories, Hercules, CA) and were created using Gibson Assembly[51] using the Gibson Assembly Master Mix (New England Biolabs ...
-
bioRxiv - Biophysics 2021Quote: ... loaded them into polyacrylamide gel wells and ran them for approximately 45 minutes at 190 V in a mini-PROTEAN tetra vertical electrophoresis cell (Bio-Rad Laboratories, Inc). We transferred the protein bands onto PVDF membranes (170-4156 ...
-
bioRxiv - Biochemistry 2020Quote: ... EV proteins were electrophoresed on a 12.5 % polyacrylamide gel (50V, 30 min) to obtain a single band (Mini-Protean II electrophoresis cell, BioRad, Marnes-la-Coquette, France). The gel was stained with Coommassie Blue and the single band of proteins was excised and subjected to in-gel digestion with bovine trypsin (Roche Diagnostics GmbH ...
-
bioRxiv - Microbiology 2020Quote: Equal amounts of protein from cell lysates were separated by SDS PAGE and transferred onto a nitrocellulose membrane by a semi-dry transfer method (Trans Blot® SD Semi-Dry Transfer cell, Bio-Rad, USA). Membranes were blocked with 5 % milk solution before incubation with primary antibodies at 1:1000 dilution unless otherwise stated ...
-
bioRxiv - Neuroscience 2020Quote: 15 μg of total protein extract from purified proteins were separated by native electrophoresis using a Tetra cell (Bio-Rad; Hercules, CA, USA), in 12% polyacrylamide separation gel and a 4% polyacrylamide stacking gel in non-denaturing conditions ...
-
bioRxiv - Biochemistry 2020Quote: Protein lysate from DENV infected and non-infected Huh7 cells (10 µg of protein as measured using Bio-Rad protein assay dye reagent) was aliquoted and purified using methanol/chloroform precipitation ...
-
bioRxiv - Cancer Biology 2021Quote: ... and gene expression of MCL-1 in the parental and MCL-1 deleted cells were verified by qPCR performed using the iTaq™ Universal SYBR Green Supermix (BioRad, California, USA), according to manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2021Quote: The protein extracts from the cells (40 μg) were subjected to electrophoresis in SDS-PAGE on 4–15% precast protein gels (Bio-Rad, Hercules, CA), separated and transferred to polyvinylidene fluoride (PVDF ...
-
bioRxiv - Developmental Biology 2021Quote: 2 μg of total protein derived from meiotic or post-meiotic germ cell populations was loaded onto a Mini-PROTEAN TGX gel (Bio-Rad, 456-8093) and separated by SDS-PAGE for 1 hour at 150V ...
-
bioRxiv - Microbiology 2021Quote: ... 40 μg of ΔORF3-E mNG RNA and 20 μg of N-gene RNA were electroporated into 8×106 Vero-ORF3-E cells using the Gene Pulser XCell electroporation system (Bio-Rad, Hercules, CA) at a setting of 270V and 950 μF with a single pulse ...
-
bioRxiv - Cancer Biology 2023Quote: ... The cell suspension then was divided equally and heated for 3 min at indicated temperatures on T100™ Thermal Cycler (Bio-Rad Laboratories). Cells were snap-frozen and thawed twice and centrifuged at 17.000 g for 15 min at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 µL of the first PCR amplification product was then used for analysis on a 2% ethidium bromide-stained agarose gel (120 minutes at 80V in a Mini-Sub® Cell GT gel chamber connected to a PowerPac™ HC (Bio-Rad) power supply ...
-
bioRxiv - Microbiology 2024Quote: ... Then 300 ng of plasmids were transformed into 100 μl of competent cells using the bacteria program on the MicroPulser Electroporator (Bio-Rad, CA, USA). After 1 h recovery culture at 37°C in 500 μl of LB ...
-
bioRxiv - Microbiology 2022Quote: ... The PCR products (700 ng) were introduced into electrocompetent cells of JW0452 carrying pKD46 by electroporation using a Gene Pulser XcellTM (BioRad, Hercules, CA, USA). The occurrence of recombination at the expected site was confirmed by PCR using primers CAT-584 and BW25113_2815723f (Table 2) ...
-
bioRxiv - Genetics 2024Quote: ... The percentage of targeted alleles within a cell population was measured with a Bio-Rad QX200 ddPCR machine and QuantaSoft software (v.1.7; Bio-Rad, Hercules, CA, USA) using the following reaction mixture ...
-
bioRxiv - Cancer Biology 2023Quote: ... 10-20 µl of the cell lysates were separated on 4-15% Mini-PROTEAN® TGX™ Stain-FreeTM Protein Gels from Bio-Rad and transferred to methanol-soaked polyvinylidene difluoride (PVDF ...
-
bioRxiv - Molecular Biology 2024Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in cell lysate was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Catalogue #: 12004167; Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
-
bioRxiv - Cell Biology 2023Quote: ... 5 µg in vitro transcripts were transfected into 5,000,000 cells resuspended in 400 µL cytomix containing 2 mM ATP and 5 mM glutathione using a Gene Pulser Electroporator (Bio-Rad, Munich, Germany). Cells were immediately transferred into 12 mL fresh medium and seeded in 96-well plates at density of 20,000 cells/well for replication assays and 12 mL/10cm dish for virus production.
-
bioRxiv - Microbiology 2022Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
-
bioRxiv - Plant Biology 2022Quote: ... was loaded onto a 10% SDS-PAGE gel and separated at 100 V for 2 h using a Mini Protean® Tetra Cell system (Bio-Rad, USA). Proteins were electro-transferred onto Immobilon TM PVDF membranes (Millipore ...
-
bioRxiv - Genetics 2022Quote: ... and transferred to an Immun-Blot™ PVDF membrane using a Trans-Blot® SD semi-dry transfer cell (BioRad Inc., CA, USA). Membranes were blocked with 5% non-fat dry milk in PBST for 2h ...
-
bioRxiv - Biochemistry 2022Quote: ... The cell suspension was transferred to a 0.2 cm gap cuvette and immediately electroporated by GENE PULSER II (Bio-Rad, Hercules, CA, USA) at 0.45 kV and 50 μF ...
-
bioRxiv - Cell Biology 2022Quote: ... Supernatants were collected and stored at −80□°C and cells were lysed in 1x Laemmli Buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA) for further analysis.
-
bioRxiv - Bioengineering 2023Quote: ... at 100V with a constant current for 60 mins at 4°C in a Bio-Rad Mini-Transfer Cell (Bio-Rad, Cat# 1703930) according to the manufacturer’s specifications ...
-
bioRxiv - Molecular Biology 2023Quote: ... or both at the University of Georgia (Athens, Georgia) Flow Cytometry Core Center using a Bio-Rad S3e cell sorter (Bio-Rad, Irvine, California).
-
bioRxiv - Microbiology 2024Quote: ... The recombinant protein was transferred to 0.45 μm nitrocellulose membranes using the Trans-Blot® Semi-Dry Transfer Cell (Bio-Rad, CA, USA), and bands were confirmed by 0.1% Amido black staining solution (ThermoFisher Scientific ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Percentage of targeted alleles within a cell population was measured with a Bio-Rad QX200 ddPCR machine and QuantaSoft software (v.1.7; Bio-Rad, Hercules, CA, USA) using the following reaction mixture ...
-
bioRxiv - Microbiology 2024Quote: ... PCR products and plasmids were transfected into T7 expressing Baby Hamster Kidney Cells (22) (BHK: BSRT7) using transfections and antibodies as in the confocal section but using a Zoe fluorescent microscope (Bio-Rad, Hercules, CA). All transfections were carried out using X2 polymer (Mirus ...
-
bioRxiv - Microbiology 2024Quote: ... and pIRF-3-Luc transfected cells were separated on a 10% SDS-PAGE gel using the Mini-PROTEAN® Tetra Vertical Electrophoresis System (Bio-Rad, USA). Following electrophoresis ...
-
bioRxiv - Synthetic Biology 2024Quote: ... PCRs were analyzed following DNA gel electrophoresis using 1.8% agarose/TAE gels with a Wide Mini-Sub Cell GT apparatus and 26-well combs (Bio-Rad, 1704469 and 1704457).
-
bioRxiv - Physiology 2024Quote: ... These protein lysates from cell lines and the animal-derived tissues were then prepared using 2X Laemmli loading buffer (Bio-Rad, Cat#1610737) and boiled for 10 minutes at 95°C.
-
bioRxiv - Biochemistry 2024Quote: rhMOG was electrophoretically separated under reducing conditions using Mini-Protean 4-15% TGX Precast gels and a Mini-Protean Tetra Cell (Biorad, Hercules, California, USA). For total protein staining ...