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Citations for Bio-Rad :
7151 - 7200 of 7510 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The solution was then pelleted to remove cell debris and the supernatant was mixed 1:1 with 2x Laemli sample buffer (Bio-Rad) and beta-mercaptoethanol ...
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bioRxiv - Neuroscience 2021Quote: ... Equal volumes (30 μg) of cell lysates from each condition were resolved by AnykD Mini-PROTEAN TGX precast gels (#456-9035, BIO-RAD) or 7.5% Mini-PROTEAN TGX precast gels (#456-8024 ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples (20 µg/lane) were electrophoresed on a 6–12% SDS polyacrylamide gel in a Mini-PROTEAN Tetra Cell (Bio-Rad) and subsequently transferred to a nitrocellulose membrane (Sartorius AG ...
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bioRxiv - Cancer Biology 2020Quote: ... and viability was monitored regularly using the Trypan Blue exclusion test on a TC20 automated cell counter (Bio-Rad, Hercules, CA). Once the cells reached ∼95% confluence ...
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bioRxiv - Cancer Biology 2020Quote: ... and growth factors in culture supernatants of control or R1881 treated LNCaP cells was determined using the Bioplex Pro Human Cytokine 17-plex assay system (Bio-Rad) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amount of proteins from each lysate were subjected to polyacrylamide gel (4-10 %) electrophoresis (PAGE) in Mini-PROTEAN Tetra cell apparatus (Bio-Rad). PAGE separated proteins were blotted onto a PVDF membrane in a semi-dry Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... 1×107 BHK21 cells were electroporated with approximately 2 μg of in vitro transcribed RNA using a GenePulser Xcell electroporator (Bio-Rad) to generate P0 virus stocks.
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bioRxiv - Neuroscience 2020Quote: ... Cell lysates were normalised after Bradford protein quantification and resolved on 4-20% Criterion TGX Precast Midi Protein gels (Bio-Rad), transferred to nitrocellulose and blocked in 5% milk in PBS-T (PBS + 0.05% Tween 20) ...
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bioRxiv - Microbiology 2020Quote: ... The proteins of an 0.1 OD600 equivalent quantity of cells were separated by SDS-polyacrylamide denaturing gel electrophoresis using precast TGX 4-15% gradient gels (Bio-Rad). The proteins were then transferred to a 0,2µm nitrocellulose membrane using Trans-Blot Turbo RTA Transfer Kit ...
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bioRxiv - Cell Biology 2020Quote: Extracellular medium and cell lysates were spotted on nitrocellulose membranes (0.45 μm) using Bio-Dot Microfiltration Apparatus (Bio-Rad, California, USA) (manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... ∼500bp fragments of sRNA factors were amplified from cDNA of ISE6 cells using primers listed in Table S1 using iProof High- Fidelity Master Mix (Bio-Rad) and the amplicons were treated with XhoI to insert them in the XhoI site of pLitmus28i (NEB) ...
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bioRxiv - Genetics 2022Quote: Template cDNA pools were generated from 1 µg RNA using cells grown under standard glucose conditions (as above) by using iScript (Bio-Rad) RT with first-strand synthesis priming from a mixture of random hexamers and oligo-dT ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lysates were resolved by SDS-PAGE (Sodium Dodecyl Sulfate PolyAcrylamide Gel Electrophoresis) using 4-20% precast gel (Biorad, Hercules, CA). Proteins were transferred by semi-dry transfer onto a PVDF (polyvinylidene fluoride ...
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bioRxiv - Cell Biology 2022Quote: ... 192 mM glycine and 20% of methanol) for 15 min before assembly in the Mini Trans-Blot transfer cell (BioRad 1703935). The transfer was done at 4°C for 16 h at 110 mA constant current ...
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bioRxiv - Biochemistry 2022Quote: ... and purified by vertical gel electrophoresis on a 6% (60:1 Acrylamide/Bisacrylamide) native gel column using a 491 Prep Cell (Bio-rad) run at 10W and 4°C ...
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bioRxiv - Microbiology 2022Quote: ... 20% (v/v) methanol) at a constant amperage of 300 mA for 90 min using a Mini Trans-Blot Cell (Bio-Rad). The membranes were incubated in NRA blocking buffer (INNOVAX ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lysates (10-50 μg) were subjected to SDS-polyacrylamide gel electrophoresis on a 4-20% precast gradient gels (BioRad, 5678094) and proteins were transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... Equivalent protein samples (cell lysates and DRG fractionized membrane and cytosol) were size separated using 10% or 4-20% SDS-PAGE gels (Bio-Rad), transferred to Immun-Blot PVDF membranes (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: Total protein extracts (5 × 106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVFD Membranes (Bio-Rad) [55] ...
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bioRxiv - Neuroscience 2020Quote: ... 40 μg protein of N13 cell lysates was loaded to 7.5% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (BioRad) and transferred to nitrocellulose membrane using the Trans-Blot® Turbo™ RTA Mini Transfer Kit (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... Flow cytometric staining and analysis was done as described [8] and acquired on ZE5 cell analyzer (Bio-Rad, Hercules, CA, USA). Analysis was performed using Flow Jo software.
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bioRxiv - Biochemistry 2020Quote: ... steroid concentrations or their ratios were normalized to the total protein content of the incubations as determined in the supernatant after cell lysis using the DC Protein Assay (Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: Extracellular medium and cell lysates were spotted on nitrocellulose membranes (0.45 μm) using Bio-Dot Microfiltration Apparatus (Bio-Rad, California, USA) (manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... 14 µL of PCR product were then transformed by electroporation in 90 µL cells (2.5 kV electric pulse for 5 ms, Bio-Rad micropulser). Cells were recovered in SOC outgrowth medium (NEB B9020S ...
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bioRxiv - Immunology 2022Quote: ... Proteins in culture supernatants and cell lysates were separated by SDS-PAGE and transferred onto PVDF membranes by using the Trans-Blot Turbo system (Bio-rad). Membranes were immunoblotted with anti-caspase-1 ...
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bioRxiv - Immunology 2022Quote: ... and RBD-specified single memory B cells were gated by IgG+RBD+ followed by single-cell sorting into 96-well PCR plates (free of DNase and RNase, Bio-Rad) containing 10μl lysis buffer (1X reverse transcriptase reaction buffer containing RNAse inhibitor) ...
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bioRxiv - Cell Biology 2022Quote: ... Around 1-5 μg of total DNA was mixed with the cell suspension and it was transferred to an ice-cold 0.1 cm gap cuvette (Bio-Rad; 1652089) for electroporation at 850V and 25 μF twice ...
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bioRxiv - Physiology 2020Quote: Electrophoresis was performed using a BioRad Mini-PROTEAN cell with 4-12% or 4-15% precast TGX gels (BioRad, Hercules, CA) in TGS running buffer ...
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bioRxiv - Microbiology 2020Quote: ... Samples were resolved by Bolt Bis-Tris Plus 4-12% gels in 1x MES SAS and transferred to PVDF membranes by a Trans-Blot Semi-Dry electrophoretic transfer cell (Bio-Rad) at 20 Volt for 30 minutes ...
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bioRxiv - Synthetic Biology 2020Quote: ... wet transfer was conducted under constant current of 0.375 A per gel using Mini-PROTEAN Tetra Vertical Electrophoresis Cell (Bio-Rad, USA) during a 120-min interval ...
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bioRxiv - Synthetic Biology 2020Quote: ... under constant electric current of 0.1 A per gel and voltage of 5-7 V using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad, USA). Alternatively ...
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bioRxiv - Bioengineering 2020Quote: ... on Tris-Tricine gels (self casted 10% gels, as described in ref53) using an Mini-PROTEAN Tetra Cell system (Bio-Rad). For heterododecamer samples tris-tricine (Laemmli ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were subjected to SDS-polyacrylamide gel electrophoresis and proteins were blotted onto nitrocellulose membranes using the Mini Trans-Blot Cell system (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... equivalent amounts of cells (~108 leptospires per lane) were re-suspended and boiled in reducing Laemmli sample buffer (BioRad, Hercules, CA), separated through 10-12.5% separating polyacrylamide mini-gels and then visualized by GelCode Blue Stain Reagent (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The second-dimension gel electrophoresis was performed in 1× TBE buffer containing 0.3 μg/ml of ethidium bromide at 6.0 V/cm for 5 hr at 4°C with buffer circulation in a Sub-cell GT electrophoresis system (Bio-Rad). DNA was transferred to Hybond-XL (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... in 1× TBE buffer at 2.0 V/cm for 21 hr at room temperature with buffer circulation in a Sub-cell GT electrophoresis system (Bio-Rad). The gel was stained with 0.5 μg/ml of ethidium bromide.
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bioRxiv - Synthetic Biology 2021Quote: ... The purity and size of the proteins were validated on a SDS-PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at –20° C.
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bioRxiv - Microbiology 2020Quote: ... proteins were blotted onto nitrocellulose membranes (Whatman, Maidstone, United Kingdom) using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) under conditions suggested by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... the capped full-length RNA and IBV-N transcripts dissolved in 400 μl PBS were co-transfected into Vero cells by electroporation (450 v, 50 μF, 3 mSec, GenePulser Xcell, BIO-RAD). After 48 h ...
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bioRxiv - Biochemistry 2021Quote: ... which contains 10 copies of the 601-positioning sequence and a linker region and purified using Model 491 Prep Cell (Bio-Rad). Annealed bubble templates and digested 601-R fragments were ligated using T4 DNA ligase (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... in 1× TBE buffer at 1.32 V/cm for 14 hr at room temperature with buffer circulation in a Sub-cell GT electrophoresis system (15 × 20 cm gel, Bio-Rad). The gel was stained with 1× TBE buffer containing 0.3 μg/ml of ethidium bromide and photographed ...
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bioRxiv - Molecular Biology 2020Quote: ... at 1.0 V/cm for ∼48 hr at 4°C with buffer circulation in a Sub-cell GT electrophoresis system (Bio-Rad). The buffer was changed every ∼24 hr.
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bioRxiv - Bioengineering 2021Quote: Prepared silk samples either in petri dish or on cardboard frame were viewed with the ZOE Fluorescent Cell Imager (Bio-Rad) in the brightfield and green channels ...
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bioRxiv - Evolutionary Biology 2020Quote: ... coli cells were prepared using 10% glycerol (Applichem) and transformed with the corresponding plasmids using a “MicroPulser” electroporator (BioRad, Hercules, US) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... pooled and subjected to two consecutive preparative isoelectric focusing (IEF) at pH 3-10 (first separation) and pH 5-7 (second separation) using a Rotofor Cell (Bio-Rad). The individual IEF fractions were harvested ...
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bioRxiv - Microbiology 2021Quote: ... cells were infected with SINV at an MOI of 2 and pictures were taken 6 hours post-infection using ZOE fluorescent cell imager (Bio-Rad). Proteins were collected with lysis buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... the cells were divided into 100 μl aliquots and heated individually at different temperatures for 3 minutes (Thermal cycler, BIO-RAD) followed by cooling for 2 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... first in 10% red blood cell (RBC) lysis buffer (Serotec, UK) for PBMCs or 1× Erythrolyse RBC Lysing Buffer (Biorad, UK) for whole blood ...
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bioRxiv - Microbiology 2021Quote: ... About 0.5 μg of plasmid DNA were mixed with the cell suspension and transferred to ice-cold 1mm gap cuvettes (Bio-Rad). Cells were electroporated in a Bio-Rad Gene Pulser Xcell system using settings ...
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bioRxiv - Genomics 2021Quote: ... and to perform merging of multiple bead barcode instances associated with the same cell (barcode merging is necessary due to the nature of the Bio-Rad SureCell scATAC-seq procedure used in this study ...