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Citations for Bio-Rad :
7251 - 7300 of 7510 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2022Quote: ... Equal amounts of each protein sample were separated on 4% to 16% Mini-Protean TGX stain-free precast gels using the Mini-Protean Tetra cell electrophoresis system (Bio-Rad). Upon the completion of electrophoresis ...
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bioRxiv - Biophysics 2022Quote: ... Both this sample and the total cell protein sample were mixed 1:1 with 2x loading buffer: 50% 4x Laemmli Sample Buffer (Bio-Rad), 30% 100 mM DTT ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown at 30°C for 3-6 days and images captured using a Chemidoc XRS+ imager (BioRad, Hercules, CA). All images were evenly adjusted in Photoshop (Adobe Systems Incorporated ...
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bioRxiv - Molecular Biology 2024Quote: Cell cultures at the log phase of growth were collected and checked for cell number and viability using TC20 automated counter (Bio-Rad). Samples with >90 % viability were processed in order to prepare single cell suspensions and diluted into the target numbers plus the multiplet rate (1000 cells plus 0.8 % per library) ...
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bioRxiv - Bioengineering 2023Quote: ... For detection, we used an anti-rabbit HRP-coupled secondary antibody (1:5000, Cell Signalling) and Western ECL substrate (#1705061, Bio-Rad. As a loading control ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 20 µg whole cell lysate was resolved in a 10 % precast Mini-PROTEAN TGX Stain-Free gel (Bio-Rad). At the end of the run ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were separated on a 15% Tris-Glycine gel with a 4% stacker using a Tris/Glycine/SDS buffer and transferred overnight onto an Immobilon-FL Transfer Membrane using a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked for one hour (0.5% Non-Fat Dry Milk in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were blotted onto methanol-activated polyvinylidene difluoride (PVDF) membranes (0.2 µm) with a Trans-Blot SD Semi-Dry Transfer Cell system (Bio-Rad Laboratories). After blotting ...
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bioRxiv - Cell Biology 2023Quote: ... proteins incubated with Mal-B were electrotransferred to PVDF membranes using a Trans-blot SD semi-dry transfer cell (Bio-Rad). Biotin incorporation was assessed by incubating blots with HRP-streptavidin (1:1000 v/v ...
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bioRxiv - Genetics 2024Quote: ... Forty micrograms of whole-cell lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Bio-Rad). The membranes were blocked with 5% nonfat dry milk in Tris-buffered saline with 0.05% Tween 20 (TBST ...
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bioRxiv - Immunology 2024Quote: ... the proteins were transferred to a polyvinylidene difluoride membrane (110V, 1h, 4°C) using a Mini Trans-Blot cell apparatus (Bio-Rad). Non-specific binding sites were blocked using PBS/Tween 20 (0.05% Tween 20 ...
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bioRxiv - Microbiology 2023Quote: ... run on a 12% SDS‒PAGE gel and transferred onto a PVDF membrane using a Trans-Blot Turbo electrophoretic transfer cell (Bio-Rad) at 25 V for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... 1×107 BHK21 cells were electroporated with approximately 2 µg of in vitro transcribed RNA using a GenePulser Xcell electroporator (Bio-Rad) to generate P0 virus stocks.
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bioRxiv - Systems Biology 2023Quote: ... by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using a Mini-Protean® Tetra Cell system (BioRad, Neuberg, Germany, 1658005EDU).
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bioRxiv - Cell Biology 2023Quote: ... and transferred to a nitrocellulose membrane (Maine Manufacturing, LLC) using a Trans-Blot SD Semi-Dry Transfer Cell apparatus (Bio-Rad). Primary antibodies were a rabbit monoclonal anti-HA (Cell Signaling ...
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bioRxiv - Molecular Biology 2023Quote: Whole cell lysates and eluates from immunoprecipitations were run on 10% SDS-polyacrylamide gels and transferred to supported nitrocellulose membrane (Bio-Rad) by standard methods ...
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bioRxiv - Bioengineering 2023Quote: ... followed by an additional wash and resuspension in Assay Buffer for readout by high-throughput cytometry (Bio-Rad ZE5 cell analyzer). Results were decoded using Nomic software ...
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bioRxiv - Microbiology 2023Quote: Total protein extracts (5×106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVDF Membranes (Bio-Rad). Immunodetection was performed using the primary antibodies ...
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bioRxiv - Microbiology 2023Quote: ... The protein samples were separated in 4 −15% acrylamide gels by SDS-PAGE using a Bio-Rad Mini-Protean Tera cell system (Bio-Rad). At the same time ...
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bioRxiv - Microbiology 2023Quote: ... the ratio of plasmid to chromosome copy number in Synechocystis 6803 or Synechococcus 7942 cells was estimated by droplet digital PCR using the QX200 system (Bio-Rad). Primer sets (F12/F13 and F14/R15 ...
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bioRxiv - Immunology 2023Quote: ... 10 µg of RNA was transfected into 4×10^6 S10-3 cells by electroporation with a Gene Pulser II apparatus (Bio-Rad) in 0.4 cm Gene Pulser cuvettes (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was extracted with RIPA buffer and analysed by SDS-PAGE followed by wet transfer using a Mini Trans-Blot® Cell (BioRad). Antibodies used for Western blots were ...
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bioRxiv - Microbiology 2023Quote: Total RNA was extracted from ∼10 x 106 cells of each experiment using PureZOL RNA Isolation reagent following the manufacturer (BIO-RAD) protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... For Western blotting equal amounts (up to 80 μg) of total cell lysates were loaded on SDS–polyacrylamide gel electrophoresis system (Bio-Rad) and transferred on nitrocellulose membrane which was proceeded for immunoblotting to detect specific proteins ...
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bioRxiv - Synthetic Biology 2023Quote: ... 50 μl of competent cells were mixed with 500 ng of the PCR fragment in an ice-cold 0.2 cm cuvette (Bio-Rad Inc.). Cells were electroporated at 2.5 kV with 25 mF and 200 Ω ...
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bioRxiv - Neuroscience 2023Quote: Cell cultures and animal tissues: 15 μg of protein extracts were loaded on 10% pre-cast gels (Bio-Rad Cat.# 4568034) and run for 1hr at 100V ...
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bioRxiv - Bioengineering 2023Quote: ... base editor cells were kept in the dark for 48 hours after light exposure and fluorescence micrographs were taken on a ZOE Fluorescent Cell Imager (Bio-Rad). To achieve optogenetic base editing of an endogenous gene ...
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bioRxiv - Immunology 2022Quote: Synchronous stimulation of the RIG-I pathway in A549- or HepG2-based cell lines was carried out using the Gene Pulser Xcell modular electro-transfection system (Bio-Rad) and a ShockPod™ cuvette chamber (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: Cell lysates prepared after sorting GFP+ cells into single tube were used in RT-qPCR analysis using SingleShot Two-Step RT-qPCR reagents (Bio-Rad) according to manufacture instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... The mixture was transferred to a pre-chilled 2 mm gap electroporator cuvette and cells were electroporated using the EC2 program (MicroPulser, Bio-Rad). Cells were then suspended in 3 ml 1/4 TSB (at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... and run on the gels at 160 volts for 60 min in 1X TBE in a Mini-PROTEAN Tetra Cell (Bio-Rad). The gels were stained in 1X TBE containing 1X SYBR Safe (Thermo Fisher ...
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bioRxiv - Biophysics 2022Quote: ... 5 µL of the soluble fractions of cell lysates were run on a 4-20% TGX Mini-Protean gradient gel (Bio-Rad) and stained using Gelcode Blue (Thermo Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins from equivalent numbers of cells were then separated by SDS-PAGE and transferred to a nitrocellulose or PVDF membrane using Trans-Blot Turbo Transfer System or Mini Trans-blot cells (Bio-Rad). Membranes were blocked in 5% milk in TBST (137 mM NaCl ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.1% SDS) and transferred onto nitrocellulose membranes (Protran) with a Trans-Blot SD semi-dry or wet transfer cell (Bio-Rad). Proteins of interest were probed using the appropriate primary and Horse Radish Peroxidase (HRP)-conjugated secondary antibodies (Jackson Immunoresearch) ...
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bioRxiv - Genomics 2022Quote: ... The nuclei pellet was resuspended in 1mL of cold DPBS followed by the quantification of nuclei using a Biorad TC20 Automated Cell Counter (Biorad #1450102).
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bioRxiv - Microbiology 2022Quote: ... ∼2 μg purified PCR products were electroporated into 50 μL electrocompetent cells at 1.75 kV using 0.1 cm cuvettes (Bio-Rad cat. 1652089) and a MicroPulser electroporator (Bio-Rad cat ...
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bioRxiv - Microbiology 2022Quote: ... The pellet obtained was dissolved in 10 μL of 20 mM Tris-HCl (pH 7.2) and 10 μL of Laemmli sample buffer (Mini-PROTEAN® Tetra Cell-Bio-Rad). The solubilized proteins were heated (95 °C ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1 μg purified linear PCR products were added to the cell mixture and proceeded electro-transformation (Gene Pulser Xcell system (Bio-Rad) and Gene Pulser/Micropulser Electroporation Cuvettes ...
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bioRxiv - Synthetic Biology 2022Quote: ... Obtained soluble and insoluble fraction (pellet of cell debris) was analyzed by SDS-PAGE using 4–20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and Precision Plus Protein Dual Color Standard (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... The recombinant plasmid was coated with 0.6 μm gold particles (1 μg DNA per 10 mg gold) and then introduced into onion epidermal cells using the particle bombardment method (PDS-1000/He particle delivery system, Bio-Rad). The epidermis was incubated on MS medium in darkness at room temperature (20–25 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Eight microliters of the micro-particle suspension (30 μl) were used for transformation of epidermal onion cells using the PDE-1000He particle gun (Bio-rad). Before transformation ...
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bioRxiv - Microbiology 2023Quote: ... with 10 μg of plasmid DNA expressing LpAsp-GFP or LpCht-GFP as well as pTrex-n-eGFP using a Gene Pulser X cell electroporator (Bio-Rad) and cuvette (2 mm gap) ...
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bioRxiv - Plant Biology 2023Quote: ... tabacum) BY-2 suspension-cultured cells and biolistic bombardment using a PDS-1000/He biolistic particle delivery system (Bio-Rad Laboratories) were performed as previously described (Banjoko and Trelease ...
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bioRxiv - Bioengineering 2022Quote: ... After transferring the proteins to nitrocellulose membrane using a transfer buffer (25 mM Tris/192 mM glycine/0.1 (w/v) SDS/20% MeOH) for 1 h at 150 mA in a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad), the membrane was saturated for 2 h at RT with 5% semi-skimmed milk in PBS-Tween (0.1% ...
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bioRxiv - Plant Biology 2023Quote: ... during 35 minutes at 200 V (Mini-PROTEAN® Tetra Cell, 4-Gel System, Bio-Rad laboratories Inc, Hercules, CA, USA) and were electroblotted onto 0.45 µm polyvinylidene difluoride (PVDF ...
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bioRxiv - Systems Biology 2023Quote: ... and 9 days post-infection of the sgRNA the cells were stained for CD81 expression and fixed for flow cytometry analysis (BioRad ZE5).
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bioRxiv - Microbiology 2023Quote: ... and resolved for 4 hours at 120V for LapA and 3 hours at 115V for MapA in Mini-PROTEAN Tetra Vertical Electrophoresis Cells (Bio-Rad) with 1x Tris/Glycine/SDS buffer (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: Samples were loaded onto 7.5% or 15% SDS-PAGE gels and electro-transferred with Trans-Blot® SD Semi-Dry Transfer Cell (BIO-RAD) at 18 V for 45 minutes ...
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bioRxiv - Physiology 2024Quote: ... Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min and the separated proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane using a wet transfer cell (Bio-Rad) at 100 mAmps (4°C overnight) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The purity and size of the proteins were validated on an SDS–PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at –20 °C.