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Citations for Bio-Rad :
7201 - 7250 of 7510 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 5 μg of plasmid DNA corresponding to each protein of interest was thoroughly mixed with 500 μL of the resuspended cells in an electroporation cuvette (Bio-Rad). This cuvette was subject to an electroporation pulse (280 V ...
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bioRxiv - Cell Biology 2020Quote: ... Larger pieces were removed with forceps and remaining cells were counted with trypan blue viability dye (Bio-Rad, cat. no. 1450021) and plated in keratinocyte growth medium (Gibco/Life Technologies ...
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bioRxiv - Genomics 2021Quote: ... RNAs were then transferred to a nitrocellulose membrane (GE Amersham HybondTM-N+) using a wet transfer system (Trans-Blot cell, Bio-Rad) at 100 V for 2 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... was incubated at 55°C for 2 h and then purified by 6% non-denaturing PAGE using a Prep Cell apparatus (Bio-Rad).
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bioRxiv - Bioengineering 2021Quote: ... proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (Cytiva) using a Trans-Blot SD Semi-Dry Electrophoretic Cell (Bio-Rad) with 1.5 mA / cm2 membrane for 1 h ...
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bioRxiv - Immunology 2021Quote: ... The proteins were fractionated on a 10% sodium dodecyl sulfate–polyacrylamide gel and transferred to polyvinylidene difluoride (PVDF) membrane using a semi dry Transfer Cell (Bio-Rad), which was run at 18 V for 1h ...
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bioRxiv - Biochemistry 2021Quote: ... The mixture was transformed into Escherichia coli SS320 cells by electroporation in a 2-mm cuvette using a Gene Pulser Xcell (Bio-Rad) with a setting of 2,400 volts ...
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bioRxiv - Biochemistry 2020Quote: ... Relative cell viability was assessed by measuring the absorbance at 450 nm using a microplate reader (Bio-Rad, Carlsbad, CA, USA). The survival rate of ACE2h cells was calculated using the following formula:
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bioRxiv - Cancer Biology 2021Quote: ... VNR was detected on cells fixed in cold methanol by CD51/61 immunocytochemistry (clone 23C6, 1:400; Bio-Rad, Oxford, UK). Resorption tracks on dentine discs were visualised by staining with 0.5% toluidine blue under reflected light ...
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bioRxiv - Cell Biology 2021Quote: ... separated in polyacrylamide gels, and transferred to nitrocellulose membranes (Maine Manufacturing, LLC) using a Trans-Blot SD Semi-Dry Transfer Cell apparatus (Bio-Rad). The following primary antibodies were used ...
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bioRxiv - Cell Biology 2021Quote: ... 10 ul of suspended cells were then added to a 10ul solution of 0.4% trypan blue in a buffered isotonic salt solution (Bio-Rad, #1450021), pH 7.3 ...
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bioRxiv - Cell Biology 2021Quote: ... but we have observed better suppression of autofluorescence at 1%.) Cells were further quenched and washed with 50 mM glycine (Bio-Rad) in DPBS (DPBS-G ...
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bioRxiv - Microbiology 2021Quote: ... 2 μl of bacterial cells standardized to 104 cells/μl were added to 48 μl of master mix and vortexed to evenly disperse cells before adding 300 μl of cold Droplet Generation Oil for Probes (BioRad 1863005). Emulsions were formed immediately after by shaking tubes at 25 Hz for 30 seconds on a Retch Mixer Mill MM 400 with adapters 11990 and 11993 (Mobio/Qiagen) ...
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bioRxiv - Molecular Biology 2021Quote: ... samples sorted to enrich for GFP-neg cells were used for qPCR analysis (BioRad iTaq Universal SYBR Green Supermix #1725120 and Biorad CFX96) with the primers described in Supplemental Table S1 ...
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bioRxiv - Microbiology 2020Quote: ... whole cell lysates were incubated at 100°C for 10 minutes in 50 μl SDS loading buffer (950 μl Bio-Rad® Laemmli buffer ...
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bioRxiv - Immunology 2021Quote: The total proteins extracted from A549 cells were fractionated in a DS-Sepharose column with a BioLogic Duo-Flow system (Bio-Rad). About 40 mg of proteins in 40 ml of 10 mM phosphate buffer (pH 7.4 ...
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bioRxiv - Immunology 2021Quote: The total proteins extracted from HFL1 cells were fractionated on DS-Sepharose columns with a BioLogic Duo-Flow system (Bio-Rad). About 40 mg of proteins in 40 ml of 10 mM phosphate buffer (pH 7.4 ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were separated on 8-12% SDS-PAGE gel and transferred onto a PVDF membrane (Bio-Rad, Hercules, CA, USA). The membrane was incubated overnight with the respective primary antibodies at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Gels were run for 2 hours and 10 min at 80V and transferred using the Trans-Blot SD Semi-Dry Transfer cell system (Bio-Rad). After transfer the LI-COR Revert 700 Total Protein Stain (LI-COR ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were added to the control wells without cells (Lanes 1 and 12) ranging from 0.125 – 2 mg/ml (BioRad 500-0202) and treated with formalin and NaOH at the same concentrations as the cells ...
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bioRxiv - Microbiology 2020Quote: ... 20 μg of total cell lysates were resolved using electrophoresis on SDS-PAGE gels and transferred onto nitrocellulose membrane (Bio-Rad). The membrane was blocked in 5% skimmed milk in Tris-buffered saline with 0.05% Tween 20 (TBST ...
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bioRxiv - Synthetic Biology 2023Quote: ... The purity and size of the proteins were validated on an SDS-PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at 4° C.
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bioRxiv - Cell Biology 2023Quote: ... whole cell lysates were quantified and aliquots of samples were resolved on a Criterion TGX Stain-Free precast gel (Bio-Rad). The membrane was blocked in 1XPBS + 0,1% Tween (1XPBS-T ...
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bioRxiv - Physiology 2022Quote: ... Images were captured for each well in a 24 well plate using a cell imager equipped with the correct LED lights (ZOE, Bio-Rad). Fluorescence intensity was analyzed and quantified using ImageJ software(22 ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were then transferred to a nitrocellulose membrane (GE Water and Process Technologies) using a wet transfer protocol and a Mini Trans-Blot Cell (Bio-Rad) transfer apparatus at 4C ...
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bioRxiv - Cell Biology 2023Quote: ... Denatured lysates were analyzed by SDS-PAGE and gels were transferred to PVDF membranes using a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked in 5% non-fat dry milk in TBS containing 0.1% Tween-20 (TBST ...
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bioRxiv - Molecular Biology 2023Quote: ... single yeast cells were gated on a forward-scatter area (FSC-A) by side-scatter area (SSC-A) plot around the clustered population (P1) on a ZE5 Cell Analyzer (Bio-Rad). P1 was then gated on a side-scatter width (SSC-W ...
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bioRxiv - Microbiology 2023Quote: ... and the gel was run at 180 V for about 35 minutes (Mini-PROTEAN electrophoresis cell and PowerPac power supply, Bio-rad). Coomassie brilliant blue (Bio-Safe Coomassie stain ...
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bioRxiv - Microbiology 2023Quote: ... Change in bacteria and host cell gDNA copies was assessed by qPCR using iTaq universal SYBR green Supermix (Bio-Rad; 1725125) in duplicate reactions ...
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bioRxiv - Microbiology 2022Quote: ... Virus pellets and clarified cell lysates were then analyzed by Western blotting using a nitrocellulose membrane and wet transfer (350 A, 75 min, Bio-Rad). Western blots were developed with 1:2,000 goat anti-gp120 polyclonal antibody (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... such that a fluorescent construct can be used as an expression reporter for cells co-transfected with a non-fluorescent construct.1 Cells were electroporated at 280 V and 950 μF using Gene Pulser X (Bio-Rad), then immediately resuspended in 6 ml medium and cultured in MatTek dishes (2 ml/dish ...
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bioRxiv - Cell Biology 2023Quote: ... 30 µg protein extract was loaded onto a 15% Bis-Tris polyacrylamide gel for electrophoresis then transfer to a PVDF membrane using a Mini Trans Blot Cell (Bio-Rad). Membranes were blocked for 1 h at 23°C in 4% milk/PBST (0.1% Tween-20 in PBS) ...
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bioRxiv - Cancer Biology 2023Quote: Formalin-fixed and paraffin-embedded 5μm sections were stained with hematoxylin and eosin (HE), DCT (Pep8h), SOX10 (clone EP268, Cell Marque, 383R-15) or CD3 (clone CD3-12, BioRad, MCA1477) antibodies using standard immunohistochemistry methods ...
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bioRxiv - Cell Biology 2023Quote: ... Transfer was performed for 50 min and a constant voltage of 120 V on a Mini Trans-Blot Cell (Biorad R) system ...
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bioRxiv - Synthetic Biology 2023Quote: ... in PBS at 40°C at concentrations of ∼15 million cells per milliliter and then loaded into wells of pre-formed phantoms consisting of 1% molecular biology-grade agarose (Bio-Rad) in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... then supernatants of samples were collected and loaded onto gels and transferred onto polyvinylidene fluoride membrane (Trans-Blot Semi-Dry Transfer Cell; Bio-Rad). Membranes were blocked in 2%BSA/TBS + 0.05% Tween-20 and incubated overnight at 4 °C with primary antibodies ...
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bioRxiv - Immunology 2023Quote: ... Even amounts of each protein sample were separated on a 8–10% polyacrylamide gel using Mini-PROTEAN Tetra Cell electrophoresis system (Bio-Rad). Upon completion of electrophoresis ...
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bioRxiv - Genomics 2023Quote: ... from cell preparations were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electrotransferred to a PVDF membrane (Bio-Rad) using a Trans-Blot Turbo™ transfer system (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... Purified CreS controls and equivalent OD600 nm units of cleared cell lysates by centrifugation were resolved on a 4-20% SDS-polyacrylamide gel (Bio-Rad). In this step ...
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bioRxiv - Systems Biology 2023Quote: ... by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using a Mini-Protean® Tetra Cell system (BioRad, Neuberg, Germany, 1658005EDU). Proteins were transferred to a nitrocellulose membrane (Carl Roth ...
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bioRxiv - Developmental Biology 2023Quote: Cell lysates from ITFG1-Myc-expressing MDA-MB-231 and non-expressing control MDA-MB-231 cells were separated in a 4-20% SDS-PAGE gel (Bio-Rad). The gels were stained with Imperial Protein Stain (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blot analysis was accomplished through SDS-PAGE of cell lysates or affinity-resin eluates in 1X laemmli loading-buffer (Bio-Rad) containing β-mercaptoethanol (1/10 in 4X buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... we electroporated a small fraction of the assembly reaction into inhouse DH10B cells using 1 mm cuvettes and a micropulser (Bio-Rad) set at 1.8 kV ...
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bioRxiv - Molecular Biology 2022Quote: ... using 10 mM CAPS buffer (3-[cyclohexylamino]-1-propanesulfonic acid [pH 11]) in a Mini Trans-Blot Electrophoretic Transfer Cell tank (Bio-Rad) according to protocol provided by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... A final concentration of 0.1 μg/mL DAPI was then added for 10 mins and digital images were captured using ZOE Fluorescent Cell Imager (Bio-Rad). For negative controls ...
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bioRxiv - Molecular Biology 2022Quote: ... 25 µg total RNA each from WT and rad6Δ cells was resolved on 15% TBE-Urea polyacrylamide gel (Bio-Rad, #3450091). The RNAs were then transferred onto positively charged nylone membrane (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 30 μg of whole-cell lysate or IP elutions were resolved on 4 to 15% mini-PROTEAN TGX gels (Bio-Rad). Transfers to PVDF membranes (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: All native electrophoresis gels were run in a Tetra Vertical Electrophoresis Chamber (Mini-PROTEAN® Tetra Cell, Bio-Rad, CA, USA). Recombinant PNPase (100 pmols ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting lysate was resolved on SDS-PAGE gel and protein was blotted to nitrocellulose membrane (Li-COR #926-31902) with mini Trans-Blot Cell (Bio-Rad). Membranes were blocked for an hour in Odyssey Blocking Buffer (TBS ...
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bioRxiv - Immunology 2022Quote: ... Even amounts of each protein sample were separated on a 4–16% Mini-PROTEAN TGX Stain-Free Precast Gels using Mini-PROTEAN Tetra Cell electrophoresis system (Bio-Rad). Upon completion of electrophoresis ...