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Citations for Bio-Rad :
7101 - 7150 of 7510 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... For each sample 20 μg of K562 cell extracts were loaded on 6-18% hand-casted acrylamide SDS-PAGE gradient gel (40% Acrylamide/bis-Acrylamide solution, BioRad). After separation by electrophoretic run ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto a 0.45 µm PVDF membrane (Carl Roth, T830.1) by using the Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad). The membrane was blocked with 5 % BSA (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... 50 µL of the cell-oligonucleotide mixture was transferred to a pre-chilled 1 mm electroporation cuvette (BioRad catalog #1652083) and transformed with 1.8 kV ...
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bioRxiv - Biochemistry 2024Quote: ... Cell lysates (30 μg) were separated on precast 4-15% or 7.5% SDS-polyacrylamide Criterion gels (Bio-Rad, Hercules, CA) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2024Quote: ... proteins were transferred onto a nitrocellulose membrane using the Mini Trans-Blot® Cell and Criterion™ Blotter (Bio-Rad). Proteins on the membrane were stained with Ponceau S buffer ...
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bioRxiv - Immunology 2024Quote: ... Stained cells were resuspended in FACS buffer and acquired with a 5-laser Bio-Rad ZE5 flow cytometer (Bio-Rad). FACS data were analyzed using FlowJo version 10.9 software (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: T cells were washed once with ice-cold PBS and immediately lysed with 4x laemmli buffer (Bio-Rad, cat. 1610747). Cell lysates were separated by SDS-PAGE and blotted with anti-Kcnn4 antibody (cat ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein purity and size were validated on an SDS-PAGE gel (Bio-Rad Mini-PROTEAN TGX and Mini-TETRA cell). Purified proteins were stored at –80°C.
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bioRxiv - Cell Biology 2024Quote: ... The cell lysates were fractionated by SDS-PAGE on a 10% gel or a 4-15% gradient gel (Bio-rad) and the separated proteins were transferred to a polyvinylidene difluoride membrane (Merck) ...
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bioRxiv - Cell Biology 2024Quote: ... and approximately 1 μg of plasmid DNA was electroporated into the cells by pulsing twice at 1000 V with the Xcell gene pulser (Biorad). The cells were incubated with subsequent addition of healing solution (2 mM CaCl2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 10 million induced cells from each track were washed and labelled for HA and c-Myc detection as described above and sorted on an S3e cell sorter (BioRad) to isolate cells that show HA detection ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were transferred from gels to the PVDF membrane using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad). Membranes were incubated in 5% nonfat dried milk in 1X TBST (Tris-buffered saline ...
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bioRxiv - Neuroscience 2024Quote: ... The gel was transferred to a 0.22 μm nitrocellulose membrane (AmershamTM Protran, G10600080) through Mini Trans-Blot Electrophoretic Transfer Cell (BioRad, 1703930) for 60 min at 100 V ...
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bioRxiv - Molecular Biology 2024Quote: Protein from the gel was transferred onto 0.45 µm nitrocellulose membrane (Bio- Rad) using a Trans-Blot SD Semi-Dry Transfer cell (Bio-Rad) at a voltage of 25 V for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.1% SDS (#50-751-6948, Quality Scientific) in nanopure water] by using the Criterion Cell electrophoresis chamber (Bio-rad). Then ...
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bioRxiv - Plant Biology 2024Quote: ... transient assay in onion cells (bulb sliced to ∼16 cm2) was conducted using the Biolistic PDS 1000/He Particle Delivery System (Biorad) (1100 psi ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were separated on a 10% SDS-PAGE and transferred to 0.2 µm nitrocellulose membrane using the Trans-blot SD semi-dry transfer cell (Bio-Rad). Blots were blocked for one hour with Odyssey Blocking Buffer ...
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bioRxiv - Microbiology 2024Quote: ... 3-5 µL of cell suspension was then deposited on a 1.5% (w/v) low melting agarose (LMA; Bio-Rad) pad prepared using a gene frame positioned on a glass slide ...
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bioRxiv - Plant Biology 2024Quote: ... was run at 120 V for 1:30 h and blotted to polyvinylidene fluoride (PVDF) membrane (Hybond P 0.45, Amersham, Cytiva, Marlborough, USA) in a Trans-Blot SD Semi-Dry Electrophoretic Cell (Bio-Rad) for 1.15 h with 1.5 mA/cm2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred onto nitrocellulose membranes (0.2 μm, Amersham Protran) for 30 min at 15V with a Trans-Blot SD semidry transfer cell (Bio-Rad) in transfer buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... cell extracts were resolved on 18% SDS-PAGE and proteins were transferred onto the 0.45µm nitrocellulose membrane (Bio-Rad, 1620115). The blots were blocked by using 2.5 % bovine serum albumin (Himedia ...
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bioRxiv - Microbiology 2024Quote: ... Samples were analyzed in 6 % Tris-glycine SDS-polyacrylamide gels, transferred on nitrocellulose membranes (Protran 0.45 μm, Amersham) using a semi-dry cell (Trans-Blot SD, Bio-Rad) in transfer buffer (39 mM glycine ...
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bioRxiv - Genetics 2024Quote: Quick extract gDNA template was prepared by depositing 5.0 x 104 cells into a 96 well hard-shell PCR plate (Bio-Rad). The plate was centrifuged ...
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bioRxiv - Immunology 2024Quote: ... the SDS-PAGE gels were transferred to an Amersham Hybond P Low fluorescence 0.2 μm PVDF membrane (Cytiva) using the Trans-blot SD semidry transfer cell (Bio-Rad) (25 V ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred on nitrocellulose membrane at 100V for 2 h using the Mini trans-blot cell (Bio-Rad, #1703930). Membranes were blocked with 5% skim milk diluted in PBST (PBS + 0.1% Tween 20 ...
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bioRxiv - Biophysics 2024Quote: ... The protein samples were analyzed for yield and purity by SDS-PAGE Criterion gels in a Dodeca cell (Bio-Rad). Proteins with visible expression by SDS-PAGE were analyzed for homogeneity by analytical size exclusion chromatography on a BioInert HPLC (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were then transferred onto a methanol-activated PVDF membrane (Cytiva) using a Mini Trans-Blot Transfer Cell (Bio-Rad) at a constant 200 mA and 4 °C for 2.5 hours in cold transfer buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... and loaded onto 10% or 12% SDS-polyacrylamide gels (SDS-PAGE) in a mini-PROTEAN Tetra electrophoresis cell (Bio-Rad). An electric field (35 mA per gel ...
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bioRxiv - Cancer Biology 2024Quote: Protein lysates were generated by sonicating and boiling 1*10^6 cells in 100ul of 2X Laemmli Buffer (Bio-Rad) with BME ...
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bioRxiv - Cancer Biology 2024Quote: ... and transferred to nitrocellulose membranes (Amersham Protran 0.45 NC, Cytiva) using a Trans-Blot SD Semi-Dry Transfer Cell apparatus (Bio-Rad). Primary antibodies were ...
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bioRxiv - Cancer Biology 2024Quote: ... with 1/12 of the volume being passed onto a 24 well plate and the remaining cells being washed with and resuspended in PBS to be electroporated (Biorad) with 10μg of PBase expression plasmid 40 and passed to a single well of a 6 well plate ...
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bioRxiv - Cancer Biology 2024Quote: ... protein concentration of cells lysed with mRIPA buffer containing protease inhibitor was measured using DC™ Protein Assay (Bio-Rad). Protein (20μg ...
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bioRxiv - Cell Biology 2024Quote: ... at a constant voltage of 120V for 65 min in a Mini-PROTEAN Tetra Cell (Cat.: 1658001, Bio-Rad, USA) filled with diluted Tris-Glycine SDS Buffer (Cat. ...
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bioRxiv - Cancer Biology 2024Quote: ... Immunoblots were visualized using the Chemiluminescent HRP Substrate kit (MilliporeSigma, Burlington, MA, USA) and the ChemiDoc™ XRS+ Molecular Imager® (Bio-Rad Laboratories, Hercules, CA, USA). Densitometry quantification of immunoblot bands was performed using ImageJ.
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bioRxiv - Cancer Biology 2021Quote: Protein samples from LNCaP cells used to measure luciferase activity (described in Fig. 4A) were loaded into 4% to 15% SDS-PAGE (Bio-Rad) and subjected to electrophoretic analysis and subsequent blotting ...
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bioRxiv - Cancer Biology 2021Quote: The prepared whole cell lysates were used to conduct gel electrophoresis followed by transfer to a polyvinylidene fluoride membrane (Bio-Rad). The membrane was blocked in 5% milk solution in Tween-PBS (PBS with 0.1% Tween 20 ...
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bioRxiv - Biochemistry 2021Quote: ... Cell sorting was performed on the Bio-Rad Sorter S3e instrument with the Software ProSort 1.5 (Bio-Rad, Hercules, California, USA).
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bioRxiv - Cell Biology 2020Quote: ... 2 mM L-glutamine and counted in the presence of Trypan Blue using the TC20™ Automated Cell counter (Bio-Rad) (24h floating cell count) ...
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bioRxiv - Cell Biology 2020Quote: ... 2-20 μg protein per sample were separated for 2-4 h at 60 – 100 V using 8 % 26 well NuPAGE Bis-Tris Midi gels in MOPS or MES buffer (LifeTechnologies) and in Criterion cells (Bio-Rad) using respective adapters (LifeTechnologies) ...
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bioRxiv - Immunology 2021Quote: ... Macrophages and T cells in aortic root were stained by incubating aortic root cross-sections with the primary antibodies to CD68 (Bio-Rad) and CD3 (Abcam) ...
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bioRxiv - Genetics 2021Quote: 5 to 10 million cells were collected and suspended in the Laemmli buffer and separated on an SDS-PAGE gel (Bio-RAD). Following transfer to nitrocellulose membrane (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... Normalized volumes of samples from the different growth time points were applied on SDS-PAGE gels (10% acrylamide) and transferred to a nitrocellulose membrane using Mini Trans-Blot cells (Bio-Rad Laboratories Ltd. ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins on the gels were transferred onto PVDF membrane using BioRad Mini Trans-Blot system (BioRad, Mini Trans-Blot® Cell). The membranes were washed with pure water and blocked with 5% skimmed milk in 0.1% Tween-20/PBS (PBST ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was decanted and the cell pellet was washed twice with cold cPBS (PBS treated with Chelex-100 resin (Bio-Rad) for 2 h ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred onto nitrocellulose membranes (Amersham Protran) for 30 min at 15V with a Trans-Blot SD semidry transfer cell (Bio-Rad) or in transfer buffer (25 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... 5.0 millions of stably transduced cells were electroporated with 50 μg plasmids by the Gene Pulser X cellTM Electroporation System (Bio-Rad) at 250 V and 1500 microfarads ...
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bioRxiv - Bioengineering 2020Quote: ... of the of the cell lysate were loaded and separated by SDS–PAGE before transfer to a nitrocellulose membrane (BioRad, #1620177). Membranes were incubated with primary mouse anti-EGFR antibody (BioLegend ...
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bioRxiv - Genomics 2020Quote: ... were seeded per well in 6-well plates (2 ml per well). 24 hrs later cells were transfected with siRNA (Supp. Table 9) using SilentFect (Bio-Rad) in biological triplicates ...
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bioRxiv - Microbiology 2020Quote: ... The gel was blotted on a PVDF membrane using a semi-dry transfer unit (Trans-Blot Semi-Dry Electrophotetic transfer cell, Bio-Rad) at 25V for 45 minutes ...
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bioRxiv - Microbiology 2021Quote: ... anti-FLAG (Cell Signalling Technologies, 2908S), anti-SARS-CoV-2-ORF8 (MRCPPU, University of Dundee, DA088, [37]) and anti-Tubulin (BioRad, MCA77G). Donkey anti-rabbit IRDye 800 and goat anti-rat IRDye 680 (Li-cor Biosciences ...