Labshake search
Citations for Bio-Rad :
6901 - 6950 of 7510 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Thirty micrograms of denatured cell lysate proteins were loaded for SDS-PAGE electrophoresis (4–20% TGX gel, Bio-Rad). Primary antibodies include ALDH3B2 (Proteintech ...
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bioRxiv - Genetics 2024Quote: ... were loaded and run on a mini gel system (Mini-PROTEAN Tetra Vertical Electrophoresis Cell, Bio-Rad REF-1658004) at 200V for 35-45 minutes ...
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bioRxiv - Immunology 2024Quote: ... pre-soaked in transfer buffer (20 mM Tris, 190 mM glycine, 20% Methanol, pH 8.3) using a Mini-Transfer cell (BioRad) for 1.5 hours (100 V constant ...
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bioRxiv - Immunology 2024Quote: ... the cells were adjusted to 7.5×105 cells/mL and stimulated with recombinant human interferon gamma (IFNγ, cat. no. PHP050, BioRad) at 0.2 μg/mL for 3 h at 37°C to activate the phagocytic ability of MonoMac-6.10 Meanwhile ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins mixtures were separated using a Mini-PROTEAN Tetra Vertical Eletrophoresis Cell for Mini Precast Gels apparatus (Bio-Rad) on 4-20 % Mini-PROTEAN TGXTM Stain-Free Protein Gels (Bio- Rad ...
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bioRxiv - Microbiology 2024Quote: ... Lysed cells were resolved on a 4% to 20% Mini-Protean TGX precast protein SDS-PAGE gel (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 x 10^6 cells were electroporated with RNPs in Gene Pulser electroporation buffer (#1652676, Bio-Rad, Hercules, CA) with electroporation enhancer (#1075915 ...
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bioRxiv - Microbiology 2024Quote: ... electro-transfer was performed at a constant 20 V overnight using the Mini Trans-Blot cell module (Bio-Rad) with Dunn’s Transfer Buffer (10 mM CO3HNa ...
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bioRxiv - Microbiology 2024Quote: ... The resulting plasmids were introduced into the wild type cells of PAO1 and PA14 by electroporation (Bio-Rad MicroPulserTM) [39].
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bioRxiv - Molecular Biology 2024Quote: ... and equal amounts of cell extracts were separated by SDS 12% PAGE and transferred to nitrocellulose membrane (Bio-Rad). Samples were then analyzed by Western blotting using a rabbit-raised anti-p53 polyclonal antibody (CM1 ...
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bioRxiv - Developmental Biology 2024Quote: ... including either Goat Anti-Rabbit IgG (H+L)-HRP Conjugate (1/50,000; Bio-Rad #1721019 or Cell Signaling #7074) or Goat Anti-Mouse IgG (H+L)-HRP Conjugate (1/50,000 ...
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bioRxiv - Neuroscience 2024Quote: ... the relative mtDNA copy number per cell was quantified by a multiplex Taqman qPCR assay (Bio-Rad, Hercules, USA).23 B2M transcript levels were used as nuclear-encoded reference gene ...
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bioRxiv - Biochemistry 2024Quote: ... Protein content of whole cell lysates was determined using the DC protein Assay (Bio-Rad, Cat # 5000113 and 5000114) with absorbance at 750 nm measured on a CLARIOstar plate reader ...
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bioRxiv - Plant Biology 2024Quote: Agrobacterium electrocompetent cells were prepared and used as specified in the BioRad MicroPulser™ (Biorad Laboratories, Hercules CA, US) manual unless otherwise noted ...
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bioRxiv - Neuroscience 2024Quote: ... The separated proteins were then transferred onto nitrocellulose membrane using a Trans Blot Mini Cell (Bio-Rad Cat# 1703930) at 100V for 1h ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto nitrocellulose blotting membrane (Amersham, Cytiva) in a Mini Trans-blot ® cell system (Bio-Rad). After transfer ...
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bioRxiv - Cell Biology 2024Quote: ... The gel was run in a Bio-Rad Criterion Cell in 1x Tris/Glycine/SDS Buffer (Bio-Rad, #1610732). After running the gel ...
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bioRxiv - Cell Biology 2024Quote: ... The constructed plasmid was transfected into immortalized PDPN-expressing stromal cells by electroporation using Gene Pulser Xcell (Bio-Rad). Transfectants were isolated by limiting the dilution ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to a nitrocellulose membrane using the Trans-Blot® Turbo™ RTA Mini Nitrocellulose Transfer Kit and Trans-Blot© Turbo™ Transfer System (Bio-Rad). Antibody detection was carried out with the SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Precision Plus Protein™ Dual Color Standards and Trans-Blot® Turbo™ RTA Midi Nitrocellulose Transfer Kit were purchased from Bio-Rad (Hercules, CA). Antibodies against Phospho-c-Raf (Ser338) ...
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Glutathione S-transferase: A Candidate Gene for Berry Color in Muscadine Grapes (Vitis rotundifolia)bioRxiv - Genetics 2020Quote: ... Total RNA from berry skins and seeds was used for the synthesis of first strand cDNA using iScript cDNA synthesis kit (Bio-Rad Laboratories Inc., Hercules, CA) and diluted 1:10 for qPCR reactions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the 1st cDNA was synthesized from 200 ng of total RNA of stomach origin by using oligo-dT primers and an iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA). The sequences of primers for cDNA cloning were designed based on the genome resource (see Table 2) ...
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bioRxiv - Microbiology 2020Quote: ... Levels of the following 27 cytokines were analyzed using a BioPlex Pro™ Human Cytokine 27-plex Assay kit (#M500KCAF0Y, Bio-Rad, Hercules, CA, USA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and cDNA was synthesized from total RNA with the High Capacity cDNA Reverse Transcription kit (Applied Biosystems; Thermo Fisher. Real-time PCR was performed with iTaq Universal SYBR Green Supermix (Biorad; Cat No. 1725121) or SYBR Green PCR master mix (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... 100 ng aliquots of RNA before and after depletion were subjected to qPCR using the iTaq™ Universal SYBR® Green One-Step kit (Bio-Rad, Hercules, CA) [22] ...
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed with C-kit variant 1 forward primer 5’-CAACAAAGAGCAAATCCATCCC-3’ and reverse primer 5’-CATCACAATAATGCACATCATGCC-3’ with iQ SYBR Green supermix (BioRad, Hercules, CA, Cat No. 1708882). The PCR program was as follows ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was generated by reverse transcription of the total RNA samples with iScriptRT Advanced cDNA Synthesis Kit for RT-PCR (Bio-Rad, Hercules, CA; #170–8891;). qRT-PCR was performed in triplicates using the iTaqTM Universal SYBR® Supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: Commercially available TaqMan assays (Table S2) for droplet digital PCR were prepared using the One-Step RT-ddPCR Advanced Kit for Probes (Bio-Rad, Hercules, CA, USA; 1864022). Each 20-μL reaction mix consisted of 10 μL ddPCR Supermix for probes (no dUTP ...
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bioRxiv - Microbiology 2022Quote: ... Protein samples were resolved in 10% SDS-polyacrylamide gels and transferred to nitrocellulose membranes using the Trans-Blot Turbo RTA Midi Nitrocellulose Transfer Kit (#1704271 from Bio-Rad Laboratories, Hercules, CA, USA). Phosphorylation of MAPKs Slt2 and Fus3 was detected using a rabbit antiPhospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Neuroscience 2020Quote: ... Uptake was normalized to total mg of protein per sample measured via Bio-Rad DC protein assay kit (5000112 Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Neuroscience 2021Quote: ... was resuspended in 1 ml of PBS/Ca+/Mg+ buffer and protein content was quantified by DC protein assay kit (Bio-Rad Laboratory, Hercules, CA, USA). 500 µg of synaptosomes were incubated 60 min at 4 °C with sulfo-NHS-biotin (1.5 mg/ml ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... aliquots of the samples were organized in a 96-well PCR plate and reverse transcribed to cDNA using the iScript™ Reverse Transcription Supermix kit and protocol (Bio-Rad Laboratories, Hercules, CA). The resulting 96-well plate with cDNA was used as source quantitative real-time polymerase chain reaction (qRT-PCR ...
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bioRxiv - Genetics 2021Quote: ... equal amounts of cDNA were synthesized from 300 ng of total RNA for each sample by using the iScript™ cDNA Synthesis Kit (Bio-Rad, Hercules, CA, USA). Thirty nanograms of cDNA per reaction were analyzed for semi-qPCR using the SsoAdvanced™ Universal SYBR® Green Supermix Kit (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: Total RNA from snap-frozen IECs was isolated with peqGOLD RNAPure™ (Erlangen, Germany) and reverse transcription was performed using the iScript cDNA Synthesis Kit (Bio-Rad, Hercules, California, USA). Following ...
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bioRxiv - Immunology 2022Quote: ... produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-Rad, Marnes-La-Coquette, France). Mouse IgG1 isotype control was purchased from Miltenyi Biotec (Bergisch Gladbach ...
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bioRxiv - Physiology 2022Quote: mRNA expression was analyzed by quantitative RT–PCR as previously described (49) using cDNA synthesis kits and SYBR green mix from Bio-Rad (Cat# 1708897 and 1725121) performed with the StepOne Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA synthesis of 500 ng of RNA in a final volume of 20 μL was performed using iScript cDNA synthesis kit (Bio-Rad Laboratories, Hercules, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Bio-Rad MyiQ was used to quantify the DNA concentration of the library using the Kapa iCycler qPCR kit (Bio-Rad Laboratories, Hercules, CA, USA). Sequencing was performed on the Illumina NextSeq 500 Platform (Illumina ...
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bioRxiv - Immunology 2024Quote: ... Viral genome was quantified using a standard curve of known viral titer following qRT-PCR with iTaq Universal SYBR Green One-Step Kit (BioRad, cat#172-5151, Hercules, CA) and the following primers ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were then incubated with Omni-ECL™Femto Light Chemiluminescence Kit (Epizyme, China) and captured using the Molecular Imager® Gel Doc™ XR System (Bio-Rad, USA). The antibodies used were as follows ...
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bioRxiv - Molecular Biology 2023Quote: To prepare the tubing for gene gun delivery one tubing and 25 mg of Au particles with 1 µm Ø was used from Helios Gene Gun Optimization Kit (Bio-Rad, Hercules, CA, USA). 10 µg of the synthesized RNA transcript was used four labeling Au particles ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentrations were analyzed using a Quick Start Bradford Protein Assay Kit with bovine serum albumin as a standard (Bio-Rad Laboratories, Hercules, CA, USA). The samples were standardized by diluting in the diluent ...
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bioRxiv - Cancer Biology 2024Quote: ... and quantitative PCR was performed using a KOD SYBR qPCR kit (TOYOBO) on a CFX96 Real-Time PCR System (Bio-Rad Laboratories, Hercules, CA, USA). PCR conditions were as follows ...
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bioRxiv - Microbiology 2024Quote: ... Then, 3 mL of each culture were pelleted (11,000 rpm, 1 min) to perform total RNA extraction (using BioRad’s Aurum™ Total RNA Mini Kit). RNA concentration and purity were assessed using a nanodrop and all samples shown to have A260/A280 and A260/A230 ratios ≥ 2 ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting total protein was quantified using the Bio-Rad DC Protein Assay Kit according to the manufacturer’s recommended protocol (Bio-Rad, Cat#5000111, Hercules, CA, USA). Samples and standards were read on a BioTek Synergy HT plate reader after a 15 min incubation period ...
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bioRxiv - Neuroscience 2024Quote: mRNA expression was analyzed by quantitative RT–PCR as previously described using cDNA synthesis kits and SYBR green mix from Bio-Rad (Cat# 1708897 and 1725121) performed with the StepOne Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... The titers of the purified recombinant AAVs were determined by quantitative PCR using the iQ SYBR Green Supermix kit (Bio-Rad, Hercules, CA, USA, 1708884) with primer WPRE-F (5’-ATGCCTTTGTATCATGCTATTGCT-3’ ...
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bioRxiv - Plant Biology 2024Quote: ... was conducted to analyze the expression of genes using TransStart Green qPCR SuperMix Kit (TransGen Biotech, China) in the CFX96 machine (Bio-Rad Company, Hercules, CA, USA). The rice OsACTIN gene (LOC_Os03g50885 ...
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bioRxiv - Developmental Biology 2022Quote: ... Equal amounts of RNA for all samples in Fig 3N were simultaneously transcribed into cDNA using iScript cDNA synthesis kit (BIO-RAD, Hercules, CA, cat#170-8891) according to the manufacturer’s protocol within 7 days of the initial dissections to preserve sample quality ...
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bioRxiv - Cancer Biology 2022Quote: ... mRNA expression levels of above-mentioned genes were estimated using iTaq™ Universal SYBR® Green One-step qRT-PCR kit (#1725151, Bio-Rad Laboratories Inc., USA) per manufacturer’s protocol ...