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Citations for Bio-Rad :
6851 - 6900 of 7510 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Equal mass of each cell extract (20 μg) was separated on a 4-20% Mini-PROTEAN TGX gel (BioRad) and then transferred to Immobilon P membrane (Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was then centrifuged through a 0.45-micron filter at 4,000 x g and the resulting cell extract was measured using the DC-Lowry assay (BioRad). One milligram of total cellular protein was then incubated with 30 μl bed volume of anti-V5 beads (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein samples were then separated on 12 % polyacrylamide gels (Mini-Protean II electrophoresis cell, BioRad, Marnes-la-Coquette, France) and transferred to 0.2 μm nitrocellulose blotting membrane (GE Healthcare ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were transferred to methanol-treated PVDF membranes using a Mini Trans-Blot Cell wet-transfer apparatus (BIO-RAD), according to manufacturer’s specifications at 400 mA for 1.5 hours ...
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bioRxiv - Microbiology 2020Quote: ... 15 % methanol (v/v) in distilled water) for 1 hour at 100 volts (Mini Trans-Blot Cell, Bio-Rad). The nitrocellulose membrane was blocked in blocking buffer (5% (w/v ...
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bioRxiv - Genomics 2021Quote: ... To confirm that the cells were lysed we took an aliquot and stained them with Trypan Blue (Bio-Rad) to visualize under the microscope ...
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bioRxiv - Cancer Biology 2021Quote: ... Whole cell lysates were separated through 7.5% SDS-polyacrylamide gels and transferred to nitrocellulose membrane (Bio-Rad Laboratories, Inc.). Membranes were blocked with 5% milk powder in 0.1% Tween 20 in 1x Tris-Buffered Saline (TBST ...
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bioRxiv - Developmental Biology 2020Quote: ... and cells resuspended in PBS+/+ were transfected with 100-150 μg DNA constructs (Gene Pulser Xcell System; Bio-Rad; 220V ...
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bioRxiv - Genomics 2020Quote: ... The proteins were transferred onto PVDF membranes using the Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad, 170-3930) at 80 V ...
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bioRxiv - Immunology 2020Quote: ... Mono-Mac-6 effector cells previously stimulated with recombinant human IFN-ɣ (Bio-Rad, Hercules, CA, USA; cat. PHP050) at 0.2 µg ml-1 for 3h at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... Cells and plasmid DNA in solution were transferred into 0.2 cm generic electroporation cuvettes (Biorad Gene Pulser; Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2021Quote: Total protein concentration of each cell lysate sample was determined by Bradford assay with Protein Assay Dye (Bio-Rad) on a UV/Visible spectrophotometer (Ultrospec 2100 pro ...
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bioRxiv - Physiology 2023Quote: ... and transferred onto a nitrocellulose membrane using a semi-dry blot (25V, 0,22A, 50 min, Trans-Blot SD Semi-Dry Transfer Cell, #1703940, Biorad) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... and transferred to nitrocellulose membrane by semi-dry blotting using the Trans-Blot Semi-Dry Transfer Cell (Bio-Rad) at 100 V for 2 h ...
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bioRxiv - Microbiology 2023Quote: ... Lysed cells were resolved on a 4% to 20% Mini-Protean TGX precast protein SDS-PAGE gel (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... SDS PAGE was run in a Mini-Protean Tetra Vertical Electrophoresis Cell (Bio-Rad Laboratories, Incs., Hercules, CA, USA) using a 17% polyacrylamide gel and the PageRuler prestained protein ladder (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... 25 µl cell lysate and 50 µg protein for tissue lysates was loaded on 4-15% (Bio-Rad, 5678084) and 10% TGX Stain-Free Protein Gels (Bio-Rad ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were subsequently transformed by electroporation with pTS055(ThrBF267D) (0.1 cm Gene Pulser Cuvette #165-2089 and Micropulser, BioRad). Cells were recovered in SOC medium with kanamycin and 1 µM aTc for Cas9 induction at 30°C for 2 h and streaked out on to LB agar plates with kanamycin ...
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bioRxiv - Biochemistry 2023Quote: ... 25 μL of cells were added to an electroporation cuvette (0.1 cm gap, Bio-Rad, catalog no. 165-2089), and electroporated using the Ec1 setting (1.8 kV ...
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bioRxiv - Physiology 2022Quote: ... proteins were separated by running the gel in an electrophoresis chamber (Mini-PROTEAN Tetra Vertical Electrophoresis Cell, Bio-Rad) at 200 V for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and then imaged under the green channel of a ZOE Fluorescent Cell Imager (Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Neuroscience 2022Quote: ... Gel electrophoresis and membrane transfer for Western blotting was accomplished using the Mini-PROTEAN Tetra Cell system (BioRad, 1660828EDU) with 2x Laemmli sample buffer and 2-Mercaptoethanol (BME [BioRad ...
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bioRxiv - Biochemistry 2024Quote: ... The resolved proteins were transferred onto nitrocellulose membranes (Amersham) through a Trans-Blot semi-dry transfer cell (Bio-Rad). Membranes were subsequently blocked in Tris-buffered saline plus 0.1% Tween (TBS-T ...
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bioRxiv - Bioengineering 2024Quote: ... Transformation of yeast cells by electroporation was carried out as in [27] with a Gene Pulser electroporator (Bio-Rad).
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bioRxiv - Developmental Biology 2024Quote: ... Proteins in cell lysates were heated at 95[ for 5 minutes in Laemmli samples buffer (Bio-Rad, Hercules, CA), resolved by 10% SDS-PAGE ...
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bioRxiv - Cancer Biology 2023Quote: ... MDA-MB-231 cells were transfected with the CapG-GFP construct and its mutants using Transfectin (Biorad, Hercules, CA) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... through semi-dry transfer at 25 V for 30 min using Trans-BLOT SD Semi-dry Transfer Cell (BioRad). Tris-glycine buffer containing 20% methanol was used for transfer ...
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bioRxiv - Microbiology 2023Quote: ... We added 1.0 mg of various CRISPRi plasmids to the 0.2 ml fusobacterial competent cells in a chilled cuvette (0.1 cm electrode gap, Bio-Rad), allowing it to rest on ice for 10 minutes ...
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bioRxiv - Genomics 2023Quote: ... 10 μL of the mixture was then loaded onto a Dual-chamber Cell Counting Slide (Bio-Rad, Cat # 1450011), and the cells were counted using a Bio-RAD TC20 Automated Cell Counter ...
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bioRxiv - Molecular Biology 2023Quote: ... pAc5.1-dTRPA1-A and pAc5.1-EGFP were transiently co-transfected into control or dAGPS stable cells using TransFectin Lipid Reagent (BioRad). After a 24 h incubation ...
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bioRxiv - Synthetic Biology 2023Quote: ... After an incubation not exceeding five minutes the chilled DNA/cell suspension was filled into a pre-chilled 0.2-cm gap cuvette (BioRad) and electroporated using a Gene Pulse Controller electroporation system (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Competent cells were subjected to electroporation using a pulse of 2.5 kV (MicroPulser, Bio-Rad Laboratories, Hercules, CA, USA), followed by recovery in 2 mL of ½ YTSS medium for 12 hours at 30 °C ...
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bioRxiv - Cancer Biology 2023Quote: Plasmid DNA and siRNA oligonucleotides were transfected into U373 cells using electroporation with a GenePulser Xcell (Bio-Rad Laboratories). Electroporation was performed according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... GFP+ cells were captured into single tube with 100 μl lysis buffer containing Proteinase K and DNase (Bio-Rad). The sorted cells were then incubated for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... the gel was transferred onto a nitrocellulose membrane using Trans Blot Sd Semi-Dry Transfer Cell (BioRad Cat#1703940) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentrations of culture medium and cell lysate were determined by the Bradford assay (Bio-Rad, Hercules, CA). Culture medium or cell lysate samples were resuspended in SDS sample buffer containing β-mercaptoethanol and incubated at 95°C for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... Virus was then inactivated and whole cell lysates taken by the addition of 2× Laemmli buffer (Bio-Rad #161073) + 2-mercaptoethanol (Bio-Rad #1610710 ...
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bioRxiv - Plant Biology 2022Quote: ... Protein extracts were separated on homemade 4-20% gradient SDS polyacrylamide gels and blotted to a nitrocellulose membrane (Amersham Protran 0.2 μm NC) using a Trans-Blot SD Semi-Dry Transfer Cell (BioRad). Blotting efficiency was assessed by staining total protein with Ponceau S ...
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bioRxiv - Biochemistry 2022Quote: ... whole cell lysates were separated by SDS-PAGE and transferred to 0.45 um Immun-Blot PVDF membranes (BIO-RAD). Membranes were then blocked in 5% milk/TBST for 1 hour at room temperature and incubated with primary antibodies at 4 °C overnight ...
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bioRxiv - Immunology 2023Quote: ... RNA from the barcoded cells for each sample was reverse transcribed in a C1000 Touch Thermal cycler (Bio-Rad), and libraries generated according to the manufacturer’s protocol with no modifications (14 cycles used for cDNA amplification) ...
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bioRxiv - Microbiology 2023Quote: ... Infected cells were then washed three times with PBS and lysed in 1x Laemmli Sample Buffer (Bio-Rad #1610747) containing 100mM DTT ...
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bioRxiv - Cancer Biology 2023Quote: ... and equal amounts of cell extracts were separated by SDS 12% PAGE and transferred to nitrocellulose membrane (Bio-Rad). Samples were then analyzed by Western blotting using a rabbit-raised anti-p53 polyclonal antibody (CM1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates (50 µg) were loaded into 4–20% PROTEAN® TGX™ Precast Protein Gels (BioRad, Cat# 5678081). After overnight transfer to nitrocellulose membranes ...
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bioRxiv - Microbiology 2023Quote: ... using 2 mm electroporation cuvettes (Cell Projects, EP-102) and electroporation program Ec2 (V = 2.5 kV) with MicroPulser Electroporator (Biorad). Plasmid sequences were confirmed by sanger sequencing.
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bioRxiv - Microbiology 2023Quote: ... Each 100 μl aliquot was transformed with 0.1 pmol of EZ-Tn5
Tnp Transposome using 2 mm electroporation cuvettes (Cell Projects, EP-102) and electroporation program Ec2 (V = 2.5 kV) with MicroPulser Electroporator (Biorad). Each reaction was allowed to recover in 500 μl SOC (2% tryptone ... -
bioRxiv - Microbiology 2023Quote: ... A total of 50 μL of cells was mixed with plasmids and then subject to electroporation (Bio-Rad, USA) at 2.5 kV ...
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bioRxiv - Immunology 2024Quote: ... pre-soaked in transfer buffer (20 mM Tris, 190 mM glycine, 20% Methanol, pH 8.3) using a Mini-Transfer cell (BioRad) for 1.5 hours (100 V constant ...
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bioRxiv - Genetics 2024Quote: ... Thirty micrograms of denatured cell lysate proteins were loaded for SDS-PAGE electrophoresis (4–20% TGX gel, Bio-Rad). Primary antibodies include ALDH3B2 (Proteintech ...
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bioRxiv - Immunology 2024Quote: ... the cells were adjusted to 7.5×105 cells/mL and stimulated with recombinant human interferon gamma (IFNγ, cat. no. PHP050, BioRad) at 0.2 μg/mL for 3 h at 37°C to activate the phagocytic ability of MonoMac-6.10 Meanwhile ...
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bioRxiv - Molecular Biology 2024Quote: ... and equal amounts of cell extracts were separated by SDS 12% PAGE and transferred to nitrocellulose membrane (Bio-Rad). Samples were then analyzed by Western blotting using a rabbit-raised anti-p53 polyclonal antibody (CM1 ...