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Citations for Bio-Rad :
6751 - 6800 of 7510 citations for Mouse Cell Surface Hyaluronidase CEMIP2 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: Horizontal electrophoresis chamber Wide Mini Sub Cell GT and Gel Documentation System Gel Doc XR+ (Bio-Rad, USA) were used to examine 2% agarose gels during electrophoresis and to take photographs.
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bioRxiv - Biophysics 2024Quote: ... The DNA/cell mixture was added to a chilled cuvette and electroporated using a Gene Pulser Xcell (BioRad) with the following settings ...
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bioRxiv - Cell Biology 2024Quote: ... Cell viability and counts were determined using the Trypan Blue exclusion assay (Bio-Rad Laboratories, Hercules, CA, USA) on a TC20 Automated Cell Counter (Bio-Rad Laboratories) ...
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bioRxiv - Cancer Biology 2024Quote: ... MDA-MB-231 cell lysates were used as control samples and Precision Plus Protein Dual-Color (Bio-Rad) was used as loading control ...
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bioRxiv - Cancer Biology 2024Quote: Proteins isolated from the cell-lysates (7.5-15μg) were subjected to denaturing 10% SDS-PAGE along with 10μL protein standard (Bio-Rad). Samples were electrophoresed using 1X Tris/Glycine/SDS Buffer at 100V for 60min ...
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bioRxiv - Cell Biology 2024Quote: ... Total protein concentrations of cell lysates were measured by Bradford-basis method (Protein Assay System, Bio-Rad Laboratories) and the loading protein amounts per lane were controlled ...
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bioRxiv - Cell Biology 2024Quote: ... at 90-120 Volts for 60-90 minutes in a Mini-Protean® 3 Cell (Bio-Rad Laboratories). Gel was transferred at 350 Amperes for 120 minutes onto pre-activated polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2024Quote: Viral or A549 cell extracts were prepared and mixed with 4x SDS sample buffer (Bio-Rad, CA, USA) supplemented with 2 mM DTT ...
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bioRxiv - Genetics 2024Quote: ... for 16 h at 15 V using a Mini Trans-Blot® Cell (Art. No. 1703930, Bio-Rad) and 1x NuPAGE™ Transfer Buffer (Art ...
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bioRxiv - Cell Biology 2020Quote: ... RNA template (1 μg) was prepared and reverse transcription reaction was performed with iScript Advanced cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA, USA) according to the procedure described in the product’s instruction manual ...
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bioRxiv - Molecular Biology 2021Quote: Total protein was extracted from SSLs using 350 μL of RIPA buffer and was purified using the Ready Prep 2-D cleanup kit (Bio-Rad, Hercules, CA, USA); the protein concentration was measured using the BCA protein assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... Total RNA (410 ng) was reverse transcribed into cDNA using an iScript™ Synthesis Kit on a T100™ thermal cycler (BIO-RAD, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Target proteins were detected by chemiluminescence with Clarity™ (Beta-actin and GFP) and Clarity Max™ (SP) Western ECL Substrate kit (Bio-Rad®) on the Odyssey FC system (LI-COR) ...
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bioRxiv - Cancer Biology 2020Quote: ... One μg RNA was reverse-transcribed using oligo(dT)18 and iScript Advanced cDNA synthesis kit for RT-qPCR (Bio-Rad Laboratories; Hercules, CA). Il1r1/IL1R1 and Gapdh/GAPDH qPCR was performed using specific primers and Lightcycler 480 Sybr Green I Master (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... the cDNA was synthesised using the Bio-Rad iScript™ cDNA Synthesis Kit (25 x 20 µl reactions #1708890, Bio-Rad Laboratories Inc., USA), while the qPCR reaction was performed using the Applied Biosystems PowerUp™ SYBR™ Green Master Mix (A25741 ...
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bioRxiv - Microbiology 2021Quote: ... The amount of viral RNA expressed as TCID50 equivalent per insert (TCID50e/insert) was quantified by RT-qPCR using iTaq universal probes one-step kit (Bio-Rad, catalogue no. 1725141), and a commercial mix of primers for N gene (forward primer 5’-GACCCCAAAATCAGCGAAAT-3’ ...
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bioRxiv - Neuroscience 2022Quote: ... and cDNAs were synthesized from 200 ng of RNA of each sample using iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad, Hercules, CA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Chemiluminescence reaction was initiated with Western Bright Quantum Western blotting detection kit and measured in a ChemiDocTM imaging system (BIO-RAD, Hercules, CA, USA).
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bioRxiv - Molecular Biology 2022Quote: ... and their CWD status was independently identified utilizing the Bio-Rad TeSeE Short Assay Protocol (SAP) Combo Kit (BioRad Laboratories Inc., Hercules, CA, USA). Positive RPLNs were confirmed by IHC at the Colorado State University Veterinary Diagnostic Laboratory) ...
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bioRxiv - Microbiology 2020Quote: ... IL-22 and TNF-α was analyzed by using quantitative RT-PCR (qRT-PCR) according to the Biorad iScript™ One-Step RT-PCR Kit in a CFX96 apparatus (Bio-Rad, Hercules, CA) with the following primers ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μg of total RNA was reverse transcribed with iScript cDNA Synthesis Kit and amplified by real-time quantitative PCR with IQ-SYBR Green supermix (Bio-Rad, Hercules, CA, USA). ΔΔCt method was used for relative mRNA quantization by applying the equation 2-ΔΔCt.
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bioRxiv - Genetics 2020Quote: ... and 200-500 nanograms of total RNA was programmed for cDNA synthesis using an iScript™ cDNA Synthesis Kit (#1708890 Bio-Rad; Hercules, CA). Universal SYBR Green Supermix (Bio-Rad)-based qRT-PCR was carried out in a CFX386 Touch™Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was diluted 1:30-1:50 in water and qPCR was performed using the iTaq Universal SYBR Green kit (Bio-Rad cat. no. 1725124). Each gene-specific primer and sample mix was run in triple replicates ...
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bioRxiv - Bioengineering 2021Quote: ... at the same concentration and a volume corresponding to 500 ng was added to iScript™ cDNA synthesis kit reagents (Bio-Rad cat# 1708890) for cDNA synthesis ...
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bioRxiv - Biophysics 2021Quote: ... Real-time quantitative PCR was performed with SsoAdvancedTM Universal SYBR® Green Supermix in a CFX96 Touch™ thermocycler (all kits and devices from Bio-Rad Laboratories) using 10 ng of cDNA/ reaction ...
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bioRxiv - Genetics 2021Quote: ... followed by purification with RNeasy minicolumns (Qiagen, μg total RNA was reverse-transcribed using an iScript cDNA synthesis kit (Bio-Rad, Des Plaines, IL). Assay of RNA via quantitative PCR (qPCR ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription followed by qPCR was performed in the same reaction using the Universal Probes One-Step PCR kit (Bio-Rad Laboratories, Hercules, CA) and the Taqman primers Cftr (Mm00445197_m1) ...
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bioRxiv - Immunology 2020Quote: ... and gene expression was examined with a Bio-Rad iCycler Optical System using iQ™ SYBR green real-time PCR kit (Bio-Rad Laboratories, Inc.). The data were normalized to 18S ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... Chemiluminescence signals were visualized using the enhanced chemiluminescence kit (ECL; #32106; Thermo Fischer) and a Chemidoc XRS+ scanner system (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Microbiology 2022Quote: ... cDNA was generated from 200 ng of total RNA purified from high and low heme midguts (using a Bio-Rad iScript cDNA synthesis kit) by priming the reaction with random hexamers to ensure the capture of Sgm derived transcripts ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR was performed using a SYBR Green PCR Master Mix kit on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). The reaction system (10 μL ...
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bioRxiv - Neuroscience 2023Quote: ... frozen in liquid nitrogen and stored at −80°C until the RNA isolation with Aurum Total RNA Mini Kit (Bio-Rad, Hercules, CA, USA), as described (Makarava et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... cell viability was determined using the Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) by measuring the absorbance at 450 nm using an iMarkTM microplate absorbance reader (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Physiology 2024Quote: ... The aqueous layer containing RNA was then mixed with isopropanol and loaded to Aurum Total RNA Mini Kit columns (Bio-Rad, Mississauga, ON, Canada). Total RNA was then extracted according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Up to 1000 ng of RNA was used to synthesise cDNA using the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad, USA, #1725035) following the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were physically disrupted in liquid nitrogen before adding lysis buffer from the Aurum Total RNA Mini Kit (catalog #732-6820, Bio-Rad, Hercules, CA, U.S.A.). Further homogenization was conducted using a TissueRuptor II (catalog #9002755 ...
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bioRxiv - Immunology 2024Quote: ... as detailed in “RNA/DNA extraction and sequencing.” Kars RNA expression was measured in each tumor using the iTaq Universal SYBR Green One-Step Kit (Bio-Rad Cat. No. 1725150) and the Quantstudio 6 Flex System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μg RNA was transferred into the cDNA Ecodry Premix Kit prior to the quantitative PCR program being run on ABI QuantStudio 3 with iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, 1725121). Each reaction proceeded at 50 °C for 2 min ...
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bioRxiv - Biochemistry 2024Quote: ... The total protein concentration of pooled saliva samples was measured to be approximately 20 mg/mL using the Bradford assay kit (Bio-Rad, Hercules, CA, USA) and bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 uL of the extracted genomic DNA were used for qPCR and HRM analysis using the IQ SYBR® Green Supermix kit (BioRad, Cat no. 1708882). Mutation positive male sperm were used to fertilize wildtype eggs which were saved in culture beakers and raised until next spawn ...
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bioRxiv - Physiology 2023Quote: ... The aqueous layer containing RNA was then mixed with isopropanol and loaded to Aurum Total RNA Mini Kit columns (Bio-Rad, Mississauga, ON, Canada). Total RNA was then extracted according to the manufacturer’s instructions and concentration was assessed on the nanodrop attachment for the Varioskan LUX Multimode Microplate reader (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µg of total RNA was reverse-transcribed to single-stranded complementary DNA (cDNA) using iScript cDNA Synthesis Kit (Bio-Rad Hercules, CA, USA). RT-qPCR was run on Lightcycler 480 (Roche ...
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bioRxiv - Plant Biology 2023Quote: ... The qPCR was performed using the DyNAmo ColorFlash SYBR Green qPCR Kit (Thermo-scientific) with CFX96 Touch Real-Time PCR Detection System (Bio-Rad Hercules, CA, USA) according to the manufacturer’s protocol (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... One microgram of high-quality RNA was used for the first-strand cDNA synthesis by using an iScript™ cDNA synthesis kit (Bio-Rad Laboratories, USA). Primer specificity was ensured by performing a BLASTN search of each sequence against the Solanum lycopersicum ITAGv2.40 database (Supplementary Table S1) ...
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bioRxiv - Immunology 2023Quote: ... The viral genome of the H7N1 virus was quantified using qRT-PCR that was conducted with the Bio-Rad iTaq™ Universal SYBR ® Green One-Step Kit (BioRad, California, USA) using primers designed for the detection of the M gene as published elsewhere 60.
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bioRxiv - Genetics 2023Quote: ... RNA samples were normalised to the same concentration of 250 ng/μL and used to synthesise the complementary DNA using an iScript cDNA synthesis kit (Bio-Rad, catalogue no.: 1708891) following the manufacturer’s instructions ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... Mini kit (Macherery-Magel, Germany) and used to prepare complementary DNA (cDNA) using iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad, Hercules, CA, USA) on ABS Proflex Thermal cycler (Thermo-fisher ...
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bioRxiv - Immunology 2024Quote: ... cDNA was synthesized using 100 ng of purified total RNA in a 20 μL volume reaction with an iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA), as described in (52) ...
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bioRxiv - Physiology 2024Quote: ... Purified RNA was then used for cDNA synthesis via reverse transcription as per protocol by iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA). All PCR reactions (25 µL ...
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bioRxiv - Genetics 2024Quote: ... The membranes were then incubated with ECL (Easysee Western Blot Kit, China) and visualized with an Imaging System (Bio-Rad, Universal Hood II, USA).