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Citations for Bio-Rad :
5701 - 5750 of 6619 citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Proteins were detected using anti-mouse IgG horseradish peroxidase (HRP)-conjugated secondary antibodies (1:3,000; catalog number NEF822001EA, Perkin-Elmer) and developed with Clarity Western ECL Substrates (BioRad). All antibodies were diluted in TBS-T with 1% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2021Quote: ... Chromosomes were separated on a 1% pulse-field certified agarose gel on the CHEF Mapper XA system (Bio-Rad) under the following conditions ...
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bioRxiv - Microbiology 2020Quote: ... cDNA samples were diluted 1:15 and used for qPCR with the iTaq Universal SYBR green mastermix (Biorad, #1725120). Cycle threshold values were corrected for PCR efficiency of each primer set and normalized to the hypoxanthine phosphoribosyltransferase 1 (HPRT ...
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bioRxiv - Neuroscience 2021Quote: ... and rabbit monoclonal antibody specific for Aβ42 (1:2,000, BioLegend 812101. Secondary antibodies were fluorescently labelled. Fluorescence was detected on a ChemiDoc MP system (Biorad). Immunohistochemistry was carried out as described (44) ...
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bioRxiv - Cell Biology 2021Quote: ... This was followed by incubation with a secondary HRP-conjugated anti-rabbit or anti-mouse antibody (1:2000, BioRad), depending on the origin of the primary antibody ...
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bioRxiv - Developmental Biology 2022Quote: ... We used 1 μg of RNA to produce cDNA with iScript™ Reverse Transcription Supermix (Bio-Rad, Cat#1725120). The cDNA was subjected to quantitative real-time PCR with iTaq™ Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2022Quote: ... small RNAs were isolated (18nt–30nt) from 1 μg of total RNA using 15% urea-PAGE (Bio-Rad, USA) followed by overnight precipitation using 0.3% NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... The gradient was fractionated using a Gradient Station (BioComp) coupled with an ultraviolet 254nm detector (Bio-Rad EM-1).
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bioRxiv - Microbiology 2022Quote: ... the samples were washed extensively with lysis buffer and the proteins were eluted by boiling 5 ul of the resin with 5x SDS loading buffer and the proteins were detected by western blot analysis using rabbit antibody to FLAG (1:2,000, BioRad) or mouse antibody to Myc (1:2000 ...
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bioRxiv - Biochemistry 2022Quote: ... 1 μL protein was injected onto either a self-packed buffer exchange column (P6 polyacrylamide gel, Bio-Rad Laboratories) or a prototype desalting column from Thermo Fisher Scientific and online buffer exchanged to 200 mM ammonium acetate ...
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bioRxiv - Molecular Biology 2022Quote: ... were used at a concentration of 1:2,000 and signals were visualised using Clarity Western ECL substrate (Bio-Rad) on a ChemiDoc MP (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of each RNA sample was reverse transcribed to cDNA using the iScript cDNA synthesis kit (Bio-Rad). Quantitative PCR was performed on cDNA samples using the iQ SYBRGreen Supermix (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: All analytical gels were performed in 20% polyacrylamide gels (acrylamide:bisacrylamide 19:1) using 8×12 cm plates in a mini-PROTEAN system (BioRad). Acrylamide solution was prepared in 1X TBE buffer (89 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... the tubes were dialyzed twice for 48-72h against buffer I (25mM Tris pH 7.4, 25 mM NaCl, 1 mM β-mercaptoethanol and 0.5 g of Bio-Beads (Biorad). After dialysis ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated placental CD34+ single cell suspension was incubated with human CD34- phycoerythrin (PE) (Bio-Rad; dilution 1:25), human CD45-FITC (BioLegend ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of each cDNA sample was amplified with gene-specific primers in iQ SYBR Green Supermix (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The plate was then washed two times with TBST and then incubated with a 1:5,000 dilution of secondary antibody (Goat Anti-Rabbit IgG (H+L)-HRP (BioRad)) for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... The plate was then washed with TBST and then incubated with a 1:10,000 dilution of secondary antibody (Goat Anti-Rabbit IgG (H+L)-HRP (BioRad)) for 1 hour ...
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bioRxiv - Genetics 2021Quote: ... Membranes were washed and probed with secondary antibody (1:5000, anti-Rabbit-HRP, 1706515; anti-Mouse-HRP 1706516, BioRad) in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: ... The master mix for droplet digital PCR (ddPCR) amplification included 1× ddPCR Supermix for probes (no dUTP, Bio-Rad), 0.2 1M of each primer and 0.2 1M probe (both IDT ...
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bioRxiv - Microbiology 2022Quote: ... we used 10µL of desalted assembly reaction products to transform competent E.coli S17-1 cells (mid-log cells, washed three times in deionized ice-cold water) by electroporation (V=1.8kV; MicroPulser, BioRad). After 1h recovery at 37°C in 1mL of LB ...
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bioRxiv - Pathology 2022Quote: ... The expression levels of target proteins were quantified with Quantity One 1-D Analysis Software (Bio-Rad, Hercules, CA) and normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Zoology 2019Quote: ... and specific primers (Table 1) on a CFX96 Touch™ Real-Time PCR Detection System (Biorad, Hercules, CA, USA). Actin was used as housekeeping gene normalization control (Table 1) ...
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bioRxiv - Microbiology 2019Quote: ... Blots were incubated with appropriate horseradish peroxidase -conjugated secondary antibody goat anti-rabbit immunoglobulins (1:5000, BioRad 170-6515) or goat anti-mouse immunoglobulins (1:1000 ...
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bioRxiv - Immunology 2019Quote: ... Cells were then stained for inflammasome activation using a caspase-1 FAM-YVAD-FMK FLICA probe kit (Bio-Rad). Cells were then processed for analysis by flow cytometry.
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bioRxiv - Developmental Biology 2019Quote: ... Reverse transcription was performed on 1 μg of purified RNA using the iScript Reverse Transcription Supermix kit (Bio-Rad). The cDNA was then added to SsoFast (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were then pelleted in a 1.5 mL tube and resuspended in 1 mL phosphate buffered saline pH 7.8 (Bio-Rad) (PBS) ...
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bioRxiv - Cell Biology 2019Quote: ... Target transcripts were amplified using primers listed in Table 1 and Sso Advanced Universal SYBR Green Supermix according to manufacturer’s protocol (BioRad). All qPCR reactions were assayed in triplicate for each sample and the average Cq value was used to calculate the mean expression ratio of the test sample compared to the control sample (i.e ...
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bioRxiv - Molecular Biology 2021Quote: ... Reverse transcription of 1 μg RNA/20μl was performed with the iScript™ cDNA Synthesis Kit (1708890, Bio-Rad). qPCR was performed with iTaq™ Universal SYBR® Green Supermix (1725120 ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were washed 3 times with PBS and then blocked for 1 hour at room temperature with DPBS with 0.4% Triton X-100 and 5% non-fat dry milk (BioRad). Cells were stained with primary and secondary antibodies (Table S3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were then incubated with respective anti-mouse or anti-rabbit HRP-conjugated secondary antibody (1:1000, Bio-Rad) and visualized with a chemoluminescence kit (Santa Cruz Biotechnology ...
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bioRxiv - Microbiology 2020Quote: Serum cytokines were quantified using the Bio-plex Pro Mouse Cytokine group 1 panel 8-plex kit (Biorad M60000007A). Samples were treated according to the manufacturer’s instructions and acquired on the Bio-Plex® 200 system (Biorad).
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bioRxiv - Microbiology 2020Quote: ... which was inoculated at 30°C for 1 hour and then photographed using a UV transilluminator (Bio-Rad, USA).
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bioRxiv - Microbiology 2021Quote: ... Amplification products were resolved in 1% (w/v) agarose gel and analysed with GelDoc XR+ System (Bio-Rad Laboratories). Primers and probes are listed in Table S1.
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were detected with anti-mouse HRP-conjugated secondary antibodies (1:5000; Bio-Rad, Hercules, CA, USA), and the signal was developed using Supersignal West Pico Chemiluminescent Substrate (Thermo Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... The following primary antibodies were used: rabbit anti-mouse collagen-IV (Bio-Rad Company, cat.# 2150-1470, 1:300), rat anti-mouse CD45 (BD Pharmingen ...
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bioRxiv - Genomics 2019Quote: ... All blots were visualized using a goat anti-rabbit IgG:alkaline phosphatase fusion protein at 1:3,000 dilution in conjunction with BCIP/NBT color development (Bio-Rad).
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bioRxiv - Neuroscience 2019Quote: ... First-strand cDNA synthesis was performed from 1 μg isolated RNA template using iScript RT supermix (Bio-Rad #1708841). PCR products were amplified using a CFX96 Real-Time PCR System (Bio-Rad) ...
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bioRxiv - Cell Biology 2019Quote: ... RNA (1 μg) was reverse transcribed in 20 μl reactions using the BioRad iScript cDNA Synthesis Kit (BioRad Laboratories) with 1:1 mixture of oligo-dT and random hexamers for 30 min at 42°C ...
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bioRxiv - Genomics 2021Quote: ... 1 μL diluted cDNA in a total volume of 5 μL using a CFX384 Real-Time System (Bio-Rad). For each sample we conducted three technical replicates and used their mean as the data point ...
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bioRxiv - Microbiology 2020Quote: ... 15 % methanol (v/v) in distilled water) for 1 hour at 100 volts (Mini Trans-Blot Cell, Bio-Rad). The nitrocellulose membrane was blocked in blocking buffer (5% (w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... Complementary DNA was synthesized from 1 μg total RNA with an iScript cDNA Synthesis Kit (Bio-Rad, Munich, Germany). A CFX 96 Connect™ system (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... the complexes were bound to 1 ml of the Amylose resin in a poly-prep chromatography column (Bio-Rad) and the resin was washed extensively ...
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bioRxiv - Cell Biology 2021Quote: ... The membranes were washed in TBS-Tween and incubated with the peroxidase-conjugated secondary antibody (Bio-Rad, Table 1) for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... the Netherlands) and blocked for 1 hour at room temperature using Odyssey blocking buffer (LI-COR biosciences, Bio-Rad) in a 1:1 dilution with tris-buffered saline (TBS ...
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bioRxiv - Genomics 2020Quote: ... 1 ug of mRNA was reverse-transcribed into cDNA using the iScript cDNA Synthesis kit (Bio-Rad, Hercules, CA). RNA was hydrolyzed and re-suspended in nuclease free water ...
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bioRxiv - Immunology 2020Quote: ... We used FITC-conjugated mouse anti-pig CD4 alpha (clone MIL17, Bio-Rad AbD Serotec, Puchheim, Germany, 1:20) and Alexa Fluor 647-conjugated mouse anti-pig CD8α (clone 76-2-11 ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 minute and then imaging every 10 seconds for 5 minutes on the ChemiDoc XRS+ machine (Bio-Rad). ImageLab (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μg of purified RNA was used for cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad). Real-time PCR analysis was performed using the iQ SYBR Green Supermix (Bio-Rad ...