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Citations for Bio-Rad :
5951 - 6000 of 6619 citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... or ~7.5 million cells/mL (Fig S18c) before loading into wells of preformed phantoms consisting of 1% agarose (Bio-Rad) in PBS.
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bioRxiv - Neuroscience 2022Quote: 1 μg of total RNA purified from differentiated cells was reversed transcribed using the One-step SuperScript kit (Bio-Rad). The qRT-PCR reaction was performed in a CFX96 Real-time PCR detection system (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... The following primary antibodies were used for western-blotting at 1/1,000 dilution: anti-V5 clone SV5-Pk1 (mouse, monoclonal, BioRad, #MCA1360), anti-Rpb3 clone 1Y26 (mouse ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μg of ssDNA were added to 95 μl of VSV-G-EVs in trehalose buffer and EVs then transferred to a 1 mm electroporation cuvette (BioRad) placed on ice ...
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bioRxiv - Biophysics 2022Quote: ... The mixture was then heated up to 95°C for 5 minutes and cooled down to 30°C at the rate of 1°C per minute using a thermal cycler (BioRad).
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bioRxiv - Biophysics 2022Quote: ... 2 μl of recombinant proteins and 1 μl of a molecular weight marker (Precision Plus Protein Kaleidoscope Prestained Protein Standards, BioRad) were applied to the PAGE lane (10 wells ...
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bioRxiv - Bioengineering 2022Quote: ... droplet-digital PCR was performed for 16 extracted RNA samples using the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad) and the CFX96 thermocycler (BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were washed three times and incubated with secondary antibodies: Donkey anti Sheep/Goat IgG (dilution 1:100,000, #STAR88P, Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated 3 h with a solution containing a secondary goat-HRP anti rabbit (1:10 000) (Biorad) (milk 0.5 % TBST) ...
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bioRxiv - Microbiology 2022Quote: ... HRP-linked Goat αRat antibody (Pierce 31470) was used at 1:30,000 and detected with an HRP Substrate kit (Bio-Rad) and Bio-Rad ChemiDoc imager ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated for one h at room temperature (RT) with horseradish peroxidase (HRP)-coupled secondary Abs at 1:2000 for one h (all secondary Abs from Biorad, raised in goatanti-rat HRP Cat # 5204-2504 and anti-mouse HRP Cat # 1721011) ...
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bioRxiv - Molecular Biology 2022Quote: ... The combined solution was incubated at room temperature for 1 hr and then mixed with BioBeads SM-2 resin (BioRad) overnight to remove the detergents ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... detergents and undigested protein were removed using a Bio-Scale Mini Macro-Prep High S 1 mL cartridge (Bio-Rad) on an Agilent 1100 HPLC system at 1 mL/min ...
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bioRxiv - Microbiology 2022Quote: ... Equivalent to 1×106 parasites were resolved by electrophoresis on a Mini-Protean TGX™ precast gel (Bio-rad, California). Lipoylated proteins were detected with rabbit anti-lipoic acid antibody (ab 58724 ...
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bioRxiv - Neuroscience 2023Quote: ... Free floating sections were blocked with 3% donkey serum and incubated overnight with rat anti-CD68 (1:200, Bio-Rad). Aβ deposition and CD68 staining were both developed with a Vectastain ABC kit and DAB reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... the pellets were re-dissolved in 1 M NaOH and quantified by Bradford assay (Quick StartTM Bradford Kit, BIO-RAD), and the supernatants containing cADPR were neutralized with Chloroform/Tri-n-octylamine mixture (volume ratio 3:1 ...
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bioRxiv - Plant Biology 2022Quote: ... 1 µg of total RNA were treated with iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad; https://www.bio-rad.com/) for genomic DNA digestion and first-strand cDNA synthesis ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 1 µg RNA was used for cDNA synthesis with the IScript cDNA Synthesis Kit (Bio-Rad, 1708890), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were thawed on ice and diluted 1:1000 in 1X Bradford reagent (BioRad, Hercules, CA, Cat. No. 500-0006). Standard curve was determined via serial dilutions of 1 mg/mL BSA diluted in H2O ...
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bioRxiv - Physiology 2024Quote: ... Total RNA (1 μg) was reverse transcribed to cDNA using an iScript cDNA synthesis kit (Cat no. 170884, BioRad, USA), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was then transferred to a 1 mm cuvette (ice chilled) for electroporation at 1.5 kV for 5 ms using an electroporator (Bio-Rad). Electroporated samples were mixed with 1 ml of SOC medium and transferred into a culture tube containing 1 ml of Brucella broth (BD) ...
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bioRxiv - Neuroscience 2024Quote: ... dissolved in 1X Tris-buffered saline (TBS) for 1 hour at room temperature prior to the addition of 0.05% Tween 20 (Bio-Rad) and primary antibodies overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were then washed with TBST and incubated with StarBright Blue 700 goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... 72°C (30s) and 1 cycle of 72°C for 5 min (C1000 Touch Thermal Cycler, Bio-Rad, Hercules, CA). 2–5 μl of the PCR reactions were subjected to gel electrophoresis using a 3% agarose gel in tris-borate-ethylenediamine tetraacetic acid buffer ...
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bioRxiv - Plant Biology 2024Quote: ... prior to bombardment with 10 mg 1 μm tungsten beads treated with 5 μg purified plasmid DNA in a Helios gene gun (BioRad) at 1,550 PSI ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 0.5 μM of gene-specific primers (Sup Table 1) in a CFX96 qPCR Real-Time PCR module with C1000 Touch Thermal Cycler (BioRad). After 10 minutes at 95°C ...
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bioRxiv - Developmental Biology 2024Quote: ... PVDF membranes were blocked for 1 hour in 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T, Bio-Rad), then incubated overnight at 4 °C with antibodies listed in Supplementary Table 2 in 5% BSA in TBS-T ...
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bioRxiv - Biophysics 2024Quote: ... The concentration of TP 70 was determined by measuring A230 (1 A230 unit = 240 pmol TP70) by SmartSpec Plus spectrophotometer (BIORAD). Aliquots were flash frozen and stored at −80°C.
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... The membrane was rinsed three times with TBS-T and then incubated with goat anti-mouse IgG coupled to horse radish peroxidase (1/10,000, 1706516, Bio-Rad) in TBS-T for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... HRP-conjugated secondary antibodies used were anti-rabbit and anti-mouse at a dilution of 1:2000 and immunoblots were developed using Chemidoc imaging system (ChemiDoc, BioRad).
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bioRxiv - Microbiology 2023Quote: ... or a median 750 ng (for HIV-1) of genomic DNA was combined with ddPCR Supermix for Probes (no dUTPs, BioRad), primers (final concentration 900 nM ...
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bioRxiv - Molecular Biology 2023Quote: ... PVDF membranes were blocked for 1 hr in 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T, Bio-Rad), then incubated overnight at 4°C with antibodies listed in Table S1 in 5% BSA in TBS-T ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins of the parasite pellets were extracted using lysis buffer (1 mM DTT, 2X Laemmli buffer (Bio-rad, 161-0737) and protease inhibitor cocktail (Roche ...
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bioRxiv - Microbiology 2023Quote: ... in 1× TGX buffer and then transferred to nitrocellulose membrane using a Trans-Blot® Turbo™ Transfer System (BioRad). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... Amplification was performed in the presence of 1 mM of each oligonucleotide and iTaq Universal SYBR Green Supermix (Bio-Rad). Relative amounts of targets were calculated using the ddCT method ...
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bioRxiv - Immunology 2023Quote: ... Resultant cDNA at 1:100 dilution was subject to qPCR performed using iQ SYBR Green Supermix (BioRad, Cat. No. 1708880) on a QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... Blocked membranes were incubated with HuCAL anti-bovine Mincle CRD antibodies (2.5 µg mL-1) before incubation with secondary goat-anti-human IgG F(ab’)2: HRP (STAR126P, Bio-Rad) antibody ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was applied to an equilibrated 1-ml StrepTrap HP chromatography column connected to the NGC Chromatography System (BioRad), washed with 20 CV of StrepA and elution was performed by applying a gradient concentration of StrepB (150 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... The prepared assay plate was exposed to temperature increases from 25 ⁰C to 95 ⁰C with 1 ⁰C increments for 5 s each using a CFX Connect Real-Time PCR Detection System (BioRad). SYBR fluorescence was detected after every temperature increase ...
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bioRxiv - Microbiology 2023Quote: ... Appropriate secondary-HRP conjugated antibodies in 5% Milk TBST were incubated for 1 hr at room temperature prior to development with clarity ECL (BioRad) developing reagents with a ChemiDoc Touch (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... After 3x 10 min washes in TBST appropriate secondary-HRP conjugated antibodies in 5% Milk TBST were incubated for 1 hr at room temperature and developed with a ChemiDoc Touch (BioRad) as described above.
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bioRxiv - Molecular Biology 2023Quote: ... One aliquot was used to synthesize single-stranded cDNA starting from 1 µg of total RNA using a commercially available kit (iScript cDNA Synthesis Kit, Biorad).
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bioRxiv - Biophysics 2023Quote: ... Single-stranded cDNA was synthesized from 1 µg of total RNA as template using a commercially available kit (iScript cDNA Synthesis Kit, Biorad). RT-qPCR analysis of nascent mRNA abundance was performed in duplicate using iQ SYBR Green Supermix (Biorad #1708880 ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was cut lengthwise into strips to isolate bands of particular molecular weights before being washed with TBST and incubated at room temperature for 1 H in a 5% blocking buffer solution (Biorad). The western blot strips were then incubated overnight at 4 C in a solution consisting of the primary antibody diluted into blocking buffer ...
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bioRxiv - Biophysics 2023Quote: ... Labeling efficiency was determined by measuring the absorbance of pyrene (χ346nm = 42,000 M-1cm-1) and protein concentration by 660 nm assay (Bio-Rad). An SDS-PAGE shift assay for isolating single-mSA bound ...
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were incubated 1 h with horseradish peroxidase-conjugated secondary antibodies (1:5,000) and the bands were visualized by chemiluminescence using the Clarity Western ECL substrate (Bio-Rad) and a ChemiDoc Imaging System (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... Recovered DNA suspended in molecular grade water was used for Sybr Green reactions (1 µL) on a BioRad CFX384 (BioRad). ChIP-qPCR primers (50-150 bp ...
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bioRxiv - Microbiology 2023Quote: The cDNA was diluted to 1:20 and analysed using iQ5 Real time PCR detection system (BioRad, Hercules, California, USA) with SYBR Green detection system ...
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bioRxiv - Molecular Biology 2023Quote: ... These mixtures were then heated up to 95 °C for 5 minutes and slowly cooled to room temperature at the rate of 1 °C per minute using a thermal cycler (BioRad).
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed with TBS-T (3 × 5 minutes) and incubated in horseradish peroxidase (HRP)-conjugated secondary antibody (1:1000) (BioRad) in blocking solution for 1 hour ...