Labshake search
Citations for Bio-Rad :
5501 - 5550 of 6619 citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... Secondary antibodies used are goat anti-rabbit HRP conjugated antibodies (dilution 1:10,000) (BioRad Cat. No. 1706515) and goat anti-rabbit HRP (BioRad Cat ...
-
bioRxiv - Immunology 2023Quote: ... or sensitized with 1 mg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA), or in combination for 24 hours ...
-
bioRxiv - Neuroscience 2023Quote: ... slides were heated at 37 °C for 1 min on a T100 thermal cycler (BioRad, Hercules, CA), transferred to precooled methanol (Sigma Aldrich ...
-
bioRxiv - Molecular Biology 2023Quote: ... and individual band densities were measured and compared using Quantity One 1-D analysis software (Biorad, USA). The resulting values were normalized separately for each set of time points.
-
bioRxiv - Genomics 2023Quote: ... Blots were further washed thrice with 1× TBS-T and developed using Clarity Western ECL (BioRad, #1705060). Images were captured using the ImageQuant LAS 4000 gel documentation system ...
-
bioRxiv - Microbiology 2023Quote: ... and transferred into PVDF membrane for 1 hrs at 16 V using the semi-dry method (BioRad). The membrane was blocked with 3% BSA / 5% skimmed milk in TBS ...
-
bioRxiv - Cell Biology 2023Quote: ... coli Rosetta (DE3) cells by electroporation (Bio-Rad 1 mm cuvette, 18,000 V/cm, 200V, 25 mF). Transformed E ...
-
bioRxiv - Cell Biology 2023Quote: ... The primary and secondary antibodies used were as follows: Collagen I (Bio-Rad 2150-1410, 1:300), α-SMA (Sigma A2547 ...
-
bioRxiv - Neuroscience 2023Quote: ... Bound antibody was detected by horseradish peroxidase-conjugated anti-rabbit secondary antibody (1/20000: 1705046, Bio-Rad) and developed using Clarity™ Western ECL Substrate (1705060 ...
-
bioRxiv - Genetics 2023Quote: ... 1 μg of RNA was used for cDNA synthesis with iScript cDNA Synthesis Kits (BioRad, Hercules, CA) in a 20μl reaction volume ...
-
bioRxiv - Cell Biology 2024Quote: ... 0.3 to 2.5 µg plasmid DNA were mixed thoroughly with 50 µL 1 µm gold carriers (BioRad) (40 mg/mL ...
-
bioRxiv - Cell Biology 2024Quote: ... cDNA was generated from 1 µg RNA samples using the iScript cDNA Synthesis kit (Bio-Rad, USA). qPCR reactions were prepared using 5 ml SsoAdvanced Universal SYBR Green Supermix (Bio-Rad ...
-
bioRxiv - Biochemistry 2024Quote: ... 1 µg of total RNA was converted into cDNA with iScript cDNA Synthesis Kit (Bio-Rad, 1708891) and 50 ng of cDNA was used in each reaction ...
-
bioRxiv - Neuroscience 2024Quote: ... for 30 min before incubating with an antibody against Collagen I (1:500, Bio-Rad, 2150-1908), Collagen III (1:300 ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1% agarose TBE gels and detected by trans-UV illumination using the Gel Doc XR+ (Bio-Rad) and Image Lab software (version 5.1) ...
-
bioRxiv - Immunology 2024Quote: ... cDNA was synthesized from ∼ 1 μg of extracted RNA using an iScript cDNA synthesis kit (Bio-Rad). RT-PCR was performed for IFNB and IL-1β on the Applied Biosystems PRISM 7500 qPCR machine ...
-
bioRxiv - Microbiology 2024Quote: ... the membrane was incubated with a 1:5,000 dilution of StrepTactin-horseradish peroxidase (HRP) conjugate (Bio-Rad), and 1:500 dilution of custom polyclonal antibodies (Cocalico Biologicals ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 μg of RNA was used for reverse transcription using an iScript cDNA synthesis kit (Bio-Rad). cDNA was diluted 5 times in Milli-Q water and used as a template for PCR amplification using GoTaq G2 Green Master Mix (Promega ...
-
bioRxiv - Synthetic Biology 2024Quote: ... for 1 hour and de-stained in water overnight before imaging on a ChemiDoc Imager (Bio-Rad) or a GelDoc Go Imager (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Reverse transcription and qPCR was performed using the iTaqTM Universal SYBR® Green 1-Step Kit (BioRad 1725151) on a Bio-Rad CFX Connect Real-Time System ...
-
bioRxiv - Cell Biology 2019Quote: ... blots were incubated with 1: 5000 dilution of HRP-conjugated anti-mouse/ rabbit IgG secondary antibodies (Bio-Rad) in 1% milk-TBST for another hour ...
-
bioRxiv - Cell Biology 2020Quote: ... TBST (50 mM Tris-HCl pH 7.4, 1% Triton X-100) with 5% non-fat milk (Bio-Rad) was used for blocking ...
-
bioRxiv - Developmental Biology 2021Quote: ... The secondary incubation was performed with goat anti-rabbit IgG-HRP conjugate (1:3000) (Bio-Rad, Hercules, CA) or anti-mouse IgG-HRP conjugate (1:3000 ...
-
bioRxiv - Neuroscience 2021Quote: ... 1 μg of RNA was used to make cDNA with the iScript cDNA Synthesis Kit (Bio-Rad, 1708891). All primer pairs were designed and validated in-house for efficiency and specificity ...
-
bioRxiv - Neuroscience 2021Quote: ... membranes were rinsed and incubated with the fluorescent secondary antibodies StarBright Blue 700 anti-mouse (1:5.000, BioRad) and StarBright Blue 700 anti-rabbit (1:5.000 ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 μg of extracted RNA was transcribed into cDNA using the iScript Reverse Transcription Supermix kit (Biorad, 1708840). Quantitative RT-PCR was performed using the PerfeCTa SYBR Green SuperMix (Quantabio ...
-
bioRxiv - Molecular Biology 2020Quote: ... 0.1% Tween 20) at room temperature for 1 hour and stained with 0.3 μg/mL streptavidin-HRP (BioRad) in TBS-T for 1 hour at room temperature ...
-
bioRxiv - Genomics 2020Quote: ... Secondary antibody used were an HRP-conjugated goat anti-mouse or rabbit at 1:25,000 (BioRad, Hercules, CA).
-
bioRxiv - Genetics 2019Quote: ... followed by an anti-mouse (Abliance; 1/1,000) secondary antibody and then the Clarity Western ECL reagent (BioRad). Densitometric analysis was performed on non-saturated signals using the Image Lab™ software (BioRad).
-
bioRxiv - Physiology 2019Quote: ... containing the pre-formed Cas9/sgRNA ribonucleoproteins and loaded into a 1-mm electroporation cuvette (BioRad, 165-2089). Electroporation was performed using the GenePulser Xcell (BioRad ...
-
bioRxiv - Bioengineering 2019Quote: ... PAGE separation was carried out in 1 mm vertical mini gel format (Bio-Rad Mini-PROTEAN Tetra Cell) with a discontinuous buffer system under denaturing conditions ...
-
bioRxiv - Molecular Biology 2019Quote: ... cDNA was generated from up to 1 μg of RNA with the iScript cDNA Synthesis kit (Bio-Rad). Quantitative PCR was performed using iQ SYBR Green Supermix (Bio-Rad ...
-
bioRxiv - Molecular Biology 2019Quote: ... 1 µL input and the entire IP fractions were reverse transcribed using the iScript cDNA synthesis kit (BioRad) and subjected to RT-qPCR ...
-
bioRxiv - Genetics 2019Quote: ... The PCR products were resolved on 1% agarose gels and imaged by ChemiDoc XRS+ system (Bio-Rad Laboratories). Image Lab™ Software 6.0.1 (BioRad Laboratories ...
-
bioRxiv - Plant Biology 2020Quote: ... 1 µg of total RNA was used for cDNA synthesis with the iScript™ cDNA Synthesis Kit (BioRad). PCR was performed using the SYBR-Green PCR Master mix (SYBR® Premix Ex Taq™ ...
-
bioRxiv - Physiology 2019Quote: ... Samples were then diluted to a final concentration of 1 μg/μl in 4x Laemmli buffer (Bio-Rad) and ultra-pure water and boiled for 10 minutes to denature proteins ...
-
bioRxiv - Molecular Biology 2019Quote: ... for 1 hr using a Trans–Blot device for semi-dry transfer (Bio-Rad Laboratories, Hercules, CA, USA). Membranes were then blocked for 1 hr at RT with 5% skim milk in 0,1 % PBS-Tween and incubated overnight at 4o with the following primary antibodies diluted in 5% milk ...
-
bioRxiv - Genetics 2020Quote: ... The samples were then transferred at 0.25 A for 1 hour to a PVDF Membrane (Bio-Rad 1620177). Following protein transfer ...
-
bioRxiv - Genetics 2021Quote: ... Samples were then diluted 1:5 and placed into a 96 well plate with Supermix (no dUTPs, BioRad) and the required target locus primers and MGB-NFQ probes (Applied Biosystems ...
-
bioRxiv - Cancer Biology 2021Quote: ... cDNA was prepared from 1 μg of RNA using iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA). cDNA samples were diluted 1:10 and real-time PCR was performed using IQ SYBR Green supermix and gene-specific primers in the iCycler real-time PCR detection system (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2021Quote: 2 ⨯ 106 CAR T cells collected after 24-day expansion were resuspended with 1 ⨯ Laemmli sample buffer (BIORAD) containing 2.5% β-mercaptoethanol before sonication with 50% amplitude in Sonic Dismembrator (FisherBrand) ...
-
bioRxiv - Cell Biology 2021Quote: ... 1 μg of total RNA was reverse transcribed into cDNA using iScript Reverse Transcription Supermix (1708840, Bio-Rad). Quantitative PCR was performed with 100 ng of cDNA ...
-
bioRxiv - Cancer Biology 2021Quote: ... Complementary DNA (cDNA) was synthesized with 1-5 μg of RNA using iScript cDNA synthesis kit (Bio-Rad). mRNA levels were measured using qRT-PCR with SYBR Green Master Mix (BioRad) ...
-
bioRxiv - Cell Biology 2021Quote: ... cDNA was obtained by retrotranscription of 1 μg of total mRNA with iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: Reactions were then analysed by 20% denaturing polyacrylamide gel electrophoresis (40% solution of 19:1 acrylamide:bis-acrylamide, BioRad) and 7 M urea (Sigma Aldrich) ...
-
bioRxiv - Systems Biology 2021Quote: ... Denatured cellular extracts equivalent to 1/120 OD600 were loaded onto a 7.5% precast Mini-Protean TGX (BioRad) and resolved by SDS-PAGE ...
-
bioRxiv - Physiology 2020Quote: ... exposed to UV for 1 min and total protein patterns were visualized using ChemiDoc MP Imager (Bio-Rad). The stain-free gel contains a trihalo compound which reacts with proteins during separation ...
-
bioRxiv - Molecular Biology 2020Quote: First-strand cDNA was synthesized using 1 μg of RNA with the iScript cDNA synthesis kit (Bio-Rad) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... Immunohistochemistry was performed on paraffin sections (5 μm) using antibody F4-80 (Bio-rad, France; diluted 1:100). Sections were incubated overnight at 4°C ...
-
bioRxiv - Plant Biology 2020Quote: ... 1 μg of total RNA was used to synthesize cDNA using iScript™ cDNA Synthesis Kit (Bio-Rad). Real-time RT-PCR was performed using gene specific primers (Supplementary Table 1 ...