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Citations for Bio-Rad :
5901 - 5950 of 6619 citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... in TBS-T (TBS supplemented with 0.1% (v/v) Tween-20) and 1% (w/v) Blotting-Grade Blocker (Bio-Rad) at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... was used in a 1:2,000 dilution and HRP-conjugated goat anti-mouse secondary antibody was used in a 1:3,000 dilution (172-1011, Bio-Rad). Following gDNA based PCR screening and Western blotting ...
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bioRxiv - Biochemistry 2020Quote: ... 40 μL of electrocompetent cells were transformed with 1 ng of the lesion-containing plasmid mixed with 1 ng of pVP146 (internal control for transformation efficiency) by electroporation using a GenePulser Xcell (BioRad) at 2.5 kV ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were washed 3 times for 5 minutes with TBS-T prior to incubation in ECL reagent for 1 minute and imaging using a Chemidoc MP (BioRad). Images acquired were analyzed using Image Lab software (BioRad).
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bioRxiv - Genetics 2022Quote: ... 500 ng (sorted cells) to 1 μg (unsorted cells) of total RNA was used for reverse transcription using iScript cDNA synthesis kit (Biorad). The cDNA product was diluted at 1:20 and 2 μl of diluted cDNA was used for real time PCR analysis using iQ SYBR green supermix (Biorad) ...
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bioRxiv - Genetics 2022Quote: ... followed by goat anti-mouse IgG (H+L) alkaline phosphatase (AP)-conjugated (1:3000, Bio-Rad Cat# 170-6520, RRID:AB_11125348) antibody or Alexa Fluor 546 goat anti-mouse IgG (H+L ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The mixture was transferred to an electroporation cuvette with a 1 mm gap width and transformed using a Gene Pulser Xcell electroporator (BioRad) with 1.8 kV ...
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bioRxiv - Cell Biology 2022Quote: ... The reaction for the multiplex real time PCRs contained 1× SYBR Green qPCR Master Mix (Bio-Rad, Hercules, CA, USA), 10 ng of each template ...
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bioRxiv - Pathology 2022Quote: ... with 1% Triton X-100] and the protein concentration of each lysate was determined with a colorimetric assay (Bio-Rad). After mixing with SDS-PAGE sample buffer ...
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bioRxiv - Pathology 2022Quote: ... 1 or 10 μg of total protein were loaded and separated by SDS–PAGE on 10% mini gels (Bio-Rad), and transferred into a 0.2 μm-pore size nitrocellulose membrane (#GE10600001 ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies (for further specification see key resource table, table 1) were diluted in blocking solution (F4/80 [Bio-Rad] ...
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bioRxiv - Molecular Biology 2019Quote: ... Then the membranes were incubated with goat anti-rabbit IgG (H+L) alkaline phosphatase conjugate (1:10,000 dilution) (BIO-RAD). Subsequently ...
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bioRxiv - Biochemistry 2019Quote: 40 µl of electrocompetent cells were transformed with 1 ng of the lesion-containing or lesion-free control plasmids together with 1 ng of pVP146 (transformation control) by electroporation (GenePulser Xcell(tm) from BioRad, 2.5kV – 25µF – 200Ω) ...
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bioRxiv - Microbiology 2019Quote: ... Serum was collected at day 49 and anti-M1 titers were measured by ELISA as previously described72 with a goat anti-mouse HRP-conjugated secondary antibody at 1:5000 (BioRad). Immunized and control mice were infected subcutaneously into the flank with 2×107 cfu of the AP1 (n=17 ...
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bioRxiv - Molecular Biology 2019Quote: ... SDS-PAGE gels used for this work were 10 % final concentration made with 30 % acrylamide/bis solution at a ratio of 37.5:1 (BioRad 1610158). Please note that SDS-PAGE analysis does not resolve polyP-induced shifts ...
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bioRxiv - Cell Biology 2019Quote: ... was measured and the number of Accum moieties introduced into the light chain and heavy chain of T-DM1 were estimated by reference to a logarithm plot of molecular weight versus 1/Rf for Kaleidoscope Prestained Standards (BioRad) electrophoresed under identical conditions.
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bioRxiv - Biophysics 2019Quote: RNA concentration was estimated using absorbance at 260 nm and 1 μg was reverse transcribed using iScript reverse transcription mix (Biorad) containing random primers ...
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bioRxiv - Pathology 2020Quote: ... both used at 1:1,000 dilution in blocking buffer) and fluorescent signals were acquired using with a GelDoc Imager (Bio-Rad).
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bioRxiv - Bioengineering 2019Quote: Native PAGE gels were prepared as follows: a fresh solution comprising of polyacrylamide (19:1) at final concentrations from 7 to 13% (Biorad), 0.5x TBE buffer (45 mM Tris-borate ...
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bioRxiv - Developmental Biology 2021Quote: ... was performed using 1 μl of cDNA as a template with qPCR primers (Table 2) and iTaq Universal SYBR Green Supermix (Biorad) according to the manufacturer’s instruction and analyzed using CFX Connect Real Time System (BioRad) ...
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bioRxiv - Molecular Biology 2020Quote: ... Chromosomal DNA was separated in 1% agarose/0.5 × TBE on the CHEF-DR II Pulsed Field Electrophoresis Systems (Bio-Rad) as follows ...
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bioRxiv - Immunology 2020Quote: ... Purified libraries were diluted 1:100 and quantified again by qPCR (Two 25uL reactions, 2x iQ SYBR SUPERMix (Bio-Rad, with Read 1 (5’-TATGGTAATTGT GTGYCAGCMGCCGCGGTAA-3’) ...
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bioRxiv - Pathology 2020Quote: ... The reaction for the multiplex real time PCRs contained 1× SYBR Green qPCR Master Mix (Bio-Rad, Hercules, CA, USA), 10 ng of each template ...
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bioRxiv - Physiology 2021Quote: ... Membranes were washed with 4 x 15 min TBS-T washes prior to incubation with secondary antibody (goat anti-rabbit-HRP diluted 1:10,000, Bio-Rad) for 1 h on a shaker at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... 40S and 80S fractions were quantified using Quantity One 1-D Analysis Software v.4.1.2 (Bio-Rad Laboratories, Hercules, CA). Pno1 was quantified using ImageJ Software v1.52 (100 ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated with secondary antibody for 1 h and proteins were revealed by ECL Prime (Amersham) using ChemiDoc Touch imager (Biorad).
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bioRxiv - Biochemistry 2020Quote: ... Samples (1 µg total protein) were loaded onto non-reducing 12% Mini-PROTEAN® TGX™ precast gels (Bio-Rad), and molecular weight shifts and relative band intensity used to detect the presence and relative abundance of the crosslinked complex ...
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bioRxiv - Neuroscience 2019Quote: ... conjugated secondary antibody (1:10 000) was subsequently applied and visualized by reaction with chemiluminesence reagent (Clarity Western Blotting Substrates Kit, BioRad) on radiography film (Carestream Blue X-ray film).
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bioRxiv - Microbiology 2019Quote: ... 1) 16S rRNA gene copies using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories, Hercules, CA, USA) and previously published primers targeting most bacterial 16S rRNA genes ...
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bioRxiv - Pathology 2019Quote: The target protein bands were detected with a horseradish peroxidase (HRP)–conjugated antibody (170-6515, 172-1011, 1720-1011, 1:1,000; Bio-Rad), which was visualized by chemiluminescence with Clarity Western ECL substrate (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... glycine and 0.1% (w/v) SDS in distilled water) for 1 hour at 150 volts (Mini-protean vertical electrophoresis cell, Bio-Rad). The gels were stained in Coomassie stain (45% methanol ...
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bioRxiv - Genetics 2020Quote: ... and the absorbance was monitored at 254 nm with an ultraviolet absorbance detector (Biorad model EM-1 Econo UV monitor) to record the polysome profile ...
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bioRxiv - Genetics 2020Quote: ... Each 20 microliter reaction volume contained the equivalent of 2.0 ng RNA of the template cDNA and 200 nanomoles of each primer in 1 X SYBR Green Supermix (BioRad). The cycling conditions included one step at 95°C for 3 min ...
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bioRxiv - Neuroscience 2021Quote: ... The blots were then washed 3x with TBS-T for 10 min each and incubated with the horseradish peroxidase (HRP) conjugated specific secondary antibodies (1:20,000, Bio-Rad) for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated with Amersham ECL Western Blotting Detection Reagent (Global Life Science Solutions) for 1 min and imaged on a ChemiDoc Imaging System (BioRad).
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were further incubated in peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000) (BioRad, Hercules, CA, USA) for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Staining was removed by washing the membrane in TBS-T and the membrane was blocked for 20 min using 1% Casein in TBS (BioRad). To detect PAR chains with incorporated 6-Biotin-17-NAD+ (Biolog) ...
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bioRxiv - Biochemistry 2021Quote: A 9.5% gel solution was prepared by combining 47.5 mL 30% Bis/Acrylamide solution 29:1 (BIO-RAD, #161-0156) with 41mL MilliQ Water ...
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bioRxiv - Cancer Biology 2021Quote: Click reaction mixtures and unreacted alkyne-modified oligonucleotides were separated in a 1.2 % agarose gel for 1 h at constant 120 mV and DNA bands were captured by the ChemiDoc system (BioRad) using SYBR safe mode.
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bioRxiv - Molecular Biology 2021Quote: ... membrane was washed repeatedly with PBS-T and incubated 1 h at RT with the following secondary antibody diluted 1/5000 in milk PBS-T: Goat anti-rabbit IgG HRP conjugate (170-6515; BioRad). After the same washing procedure ...
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bioRxiv - Neuroscience 2021Quote: Recombinant or mouse-extracted tau fibrils were diluted in PBS as indicated in Supplementary Figure 1 and applied to 0.2 µm nitrocellulose membrane using the Bio-Dot microfiltration apparatus (Bio-Rad). The membranes were then blocked in 5% milk TBS-Tween 20 and subsequently incubated with primary antibody overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were scanned the following day after 1 hour incubation with secondary antibodies using Odyssey CLx Imaging System or ImageQuant system (BioRad, HRP anti-rabbit or anti-mouse (CST ...
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bioRxiv - Immunology 2021Quote: ... were added to each well and incubated with cross-absorbed anti-mouse IgG HRP polyclonal antibody (diluted 1:10,000) (STAR117P, Bio-Rad) for 2h at RT on a Multiscan FC Plate Reader (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Bradford Protein Assay Reagents 1 and 2 and Precision Plus Protein All Blue Prestained Protein Standard were from Bio-Rad. The anti His-tag mouse antibody was from GenScript (cat no ...
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bioRxiv - Molecular Biology 2021Quote: ... An aliquot was removed and diluted 1:10 in 1xPBS for cell counting using an automatic counter (Biorad TC-20) with counts spot checked by manual recount using a hemocytometer as needed ...
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bioRxiv - Cell Biology 2021Quote: ... PCR amplicons were resolved in 1% agarose (Melford) TBE gels containing 1x SYBRsafe and visualised on a Chemidoc MP (BioRad). A full list of oligonucleotides and vectors are presented in Table S1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples for qPCR were prepared with primers against target genes (Table 1) and mixed with SSO Advanced Universal SYBR Green Supermix (BioRad) in 96-well plates and analyzed on a CFX96 qPCR machine (BioRad) ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNAs were diluted 1:10 and 1µl of diluted cDNA was used per qPCR reaction with iTaq Universal SYBR Green supermix (Bio-Rad) and the following pairs of primers ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized using 1 µg of total RNA and reverse-transcribed with the iScript cDNA synthesis kit (Bio-Rad). Synthesized cDNAs were then quantified by real time quantitative PCR (qPCR ...
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bioRxiv - Immunology 2022Quote: ... 2 μm paraffin-embedded kidney sections were incubated with an anti-F4/80 antibody (CI:A3-1; Bio-Rad, Feldkirchen, Germany). After incubation with biotinylated goat anti-rat IgG (Abcam ...