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Citations for Bio-Rad :
5651 - 5700 of 6619 citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 20 mM imidazole and 1 mM DTT) and were then transferred to a disposable column (Bio-Rad, USA). Protein was eluted using the elution buffer (50 mM phosphate buffer pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked for 1 h at room temperature with 5% non-fat dry milk (Bio-Rad Laboratories) in Tris-buffered saline (TBS ...
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bioRxiv - Microbiology 2023Quote: ... diluted 1:5000 in TBE for 30 minutes while shaking and imaged on Gel Doc XR+(Bio-Rad). Full-length bands for at each time point were calculated by densitometry using Image Lab software (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... 10 or 100 ng of plasmid DNA was transferred to a 1 mm MicroPulser electroporation cuvette (Bio-Rad) and cells were then electroporated at 1.8 kV ...
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bioRxiv - Neuroscience 2024Quote: ... and 1 µg of RNA was used for cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2024Quote: ... The quantity of RNA was checked on a 1% agarose gel and documented using Gel Doc (BIO-RAD). High quantity RNA from both samples estimated by absorbance A260/280 ratio was reversibly transcribed to cDNA through reverse transcriptase PCR.
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were washed thrice and incubated with appropriate secondary antibodies conjugated with HRP (Bio-rad; 1:3,000). Bands were detected by Western-Lightning Plus Enhanced Chemiluminescence (Perkin Elmer) ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight with the following primary antibodies: αWIPI2 1:3000 (mouse, Bio-Rad, Hercules, CA, MCA5780GA), αWIPI4/WDR45 1:2000 (rabbit ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked at room temperature for 1 hour in 5% nonfat dry milk (blocking grade, Bio-Rad) dissolved in Tris-buffered saline containing 0.1% Tween 20 (TBST) ...
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bioRxiv - Neuroscience 2024Quote: ... HRP detection was performed by adding 1 ml Clarity Max™ Western ECL Blotting Substrates from Bio-Rad. Arrays were imaged using BioRad X-ray imager and quantified using ImageJ ...
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bioRxiv - Cancer Biology 2024Quote: ... The quantity of RNA was checked on a 1% agarose gel and documented using Gel Doc (BIO-RAD). High quantity RNA from both samples estimated by absorbance A260/280 ratio was reverse transcribed to cDNA ...
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bioRxiv - Immunology 2024Quote: ... 1 ug of total RNA was reverse transcribed into cDNA (iScript cDNA synthesis kit, Bio-Rad cat# 1708840). cDNA was diluted 1:30 in qPCR experiments ...
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bioRxiv - Plant Biology 2020Quote: ... Real-time RT-PCR was performed using gene specific primers (Supplementary Table 1) and SYBR Green Supermix (Bio-Rad) in a CFX96 Real-Time PCR system (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Synthesis of complementary DNA (cDNA) was completed using 1 μg of RNA and the iScript cDNA synthesis kit (BioRad). Each qRT-PCR was performed as triplicate reactions with the following reaction mixture ...
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bioRxiv - Cell Biology 2019Quote: ... Immunostaining for the vitronectin receptor (VNR) was with anti-CD51/61 (clone 23C6, 1:400; Bio-Rad, Oxford, UK) and for cathepsin K with a rabbit polyclonal antibody (3368-100 ...
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bioRxiv - Cell Biology 2020Quote: All Rab34 used in steady-state assays was treated with AG 1-X8 anion exchange resin (Bio-Rad, 1401441) immediately before use to remove bound GDP ...
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bioRxiv - Genetics 2021Quote: ... Hydrolysis probe ddPCRs (20 μl volumes) consisted of 1 x ddPCR Supermix for probes without dUTP (Bio-Rad Laboratories), virus-specific primers (HHV-6B POL F and HHV-6B POL R ...
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bioRxiv - Microbiology 2021Quote: ... and 0.4-1 μg RNA was reversely transcribed using the iScript™ cDNA Synthesis Kit (Cat. # 1708890, Bio-Rad). Real-time qPCR was conducted using the iTaq™ Universal SYBR® GreenSupermix (Cat ...
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bioRxiv - Microbiology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were incubated in 5% BLOT-QuickBlocker as follows: goat anti-mouse HRP conjugate (BioRad, 1706516; 1:5000), donkey anti-rabbit HRP conjugate (Amersham ...
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bioRxiv - Biophysics 2022Quote: ... Nanodisc reconstitution was induced by removing detergent with 0.8 mg ml-1 pre-washed Biobeads SM-2 (Bio-Rad) for 2 hours with gentle agitation at RT ...
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bioRxiv - Genetics 2022Quote: ... then 1-5ng added to a PCR mix containing 11 μl 2x ddPCR Supermix for Probe (no dUTP, BioRad), 1.1 μl 20x DMBT1 probe assay (FAM-labelled) ...
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bioRxiv - Molecular Biology 2020Quote: ... the complexes were bound to 1 ml of the appropriate resin in a poly-prep chromatography column (Bio-Rad) and the resin was washed extensively with buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... Plugs were loaded on 0.8% agarose gel in 1×TAE buffer (Certified Megabase agarose, Bio-Rad, ref# 161-3109) and run in 1×TAE buffer for 50h in CHEF-DRIII System with chiller unit (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... Transformation was done chemically with heat shock at 42 °C for 30 s or by electroporation with 0.1 cm gap cuvettes at 1.8 kV for 1 s (Gene Pulser, Bio-Rad), depending on the competent cells required ...
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bioRxiv - Microbiology 2019Quote: ... The integrity of the purified DNA template was assessed by conventional agarose gel (1% [wt/vol] electrophoresis (Bio-Rad), and the purified DNA samples were stored at 4 °C until further use.
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bioRxiv - Bioengineering 2020Quote: The cDNA was diluted 1/5 with dH2O and 2.5 µL were used for qPCR analysis (10 µL total volume; BioRad, iTaq Universal SYBR Green Supermix ...
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bioRxiv - Microbiology 2019Quote: ... Immunoreactive bands were visualized by incubation with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulins (1:5000, BioRad 170-6515) or goat anti-mouse immunoglobulins (1:5000 ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were resuspended in approximately 10 μl M9 medium and spotted onto 1% low-fluorescence agarose pads (Bio-Rad) prepared in M9 medium and covered with a no1.5 glass coverslip ...
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bioRxiv - Synthetic Biology 2019Quote: ... Reverse transcription reactions were performed from 1 μg RNA in 20 μL reactions using iScript reverse transcriptase (Bio-Rad). qPCR reactions were prepared in triplicate in a final volume of 10 μL using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Immunology 2019Quote: Samples were diluted 1 in 3 in HBSPE and run through Bio-Gel® P-30 (Bio-Rad, UK) polyacrylamide gel spin columns to minimise non-specific binding ...
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bioRxiv - Synthetic Biology 2019Quote: ... Biorad) was applied for 1 h at RT and blots were developed with Carity™ Western ECL Substrate (Biorad).
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bioRxiv - Genetics 2020Quote: ... 2 mM ATP and 1 mM DTT and the reactions were stopped by addition of Laemli sample buffer (BioRad). Samples were run on precast 4-20% gradient SDS-PAGE gels (BioRad ...
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bioRxiv - Developmental Biology 2020Quote: Antibodies used in this study are specific to sheep GFP (1:500; AbD Serotec, Bio-Rad, Hercules, CA, USA), chick GFP (1:500 ...
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bioRxiv - Genomics 2021Quote: ... PFGE was performed using 1% agarose gel in 0.5X TBE (Thermo-Fisher) in a CHEF Master XA 170-3670 system (BioRad) with the following parameters ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 1 mM DTT) and transferred onto a nitrocellulose membrane using the Transblot Turbo Transfer System (Biorad, 1704150EDU). Non-specific binding sites were blocked for 1 hour with 5% dry milk or BSA in TBS-Tween 0.1% while the primary antibody (1/1000 in 1% BSA TBS-Tween 0.1% ...
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bioRxiv - Cell Biology 2021Quote: ... RNA (1 μg) was reverse transcribed into cDNA using iScript Reverse Transcription Supermix Kit (Bio-Rad, Hercules, CA, USA). The relative levels of gene transcripts compared to the control gene 18S were determined by quantitative real-time PCR using SYBER Green PCR Supermix (Bio-Rad ...
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bioRxiv - Bioengineering 2021Quote: Equivalent number of T cells (1×10e6) were lysed in Laemmli Sample Buffer with β-mercaptoethanol (Bio-Rad, CA). Total cell lysate for each sample were resolved on 12% SDS-PAGE gels and transferred to polyvinylidene fluoride membranes (Millipore ...
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bioRxiv - Biochemistry 2020Quote: ... Total RNA (1 ug) was converted to cDNA using the iScriptTM cDNA synthesis kit (# 1708891, BIO-RAD, Hercules, CA) following the manufacturer’s protocol (BIO-RAD ...
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bioRxiv - Bioengineering 2021Quote: ... Amplifications were performed in a 20 μL reaction containing 1 × ddPCR Supermix for Probes (No dUTP),(Bio-Rad, 1863024), 250 nM of probes and 900 nM of primers and 8 μL EV DNA template ...
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bioRxiv - Neuroscience 2020Quote: ... The blots were developed with a peroxidase-labeled goat anti-mouse or anti-rabbit secondary antibody (1:5,000, Biorad) and chemiluminiscence detection (MF-ChemiBIS 3.2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates prepared at a concentration of 1 mg/ml and supplemented with 4x Laemmli Sample Buffer (Bio-Rad: 1610747) supplemented with fresh 2-mercaptoethanol (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 h at room temperature and exposed to X-ray film (Kodak) or the ChemiDoc Imaging system (Biorad) after incubation with Thermo Scientific Pierce ECL or Clarity ECL (Biorad).
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bioRxiv - Physiology 2021Quote: ... membranes were rinsed three-times in TBS/0.2% Tween for 10min and incubated for 1h with goat secondary anti-rabbit or anti-mouse IgG linked to peroxidase (dilution 1:5,000; Biorad) in TBS/0.2% Tween/2% milk ...
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bioRxiv - Biochemistry 2021Quote: Reaction products were analysed by 20% denaturing polyacrylamide gel electrophoresis (made from 40% solution of 19:1 acrylamide:bis-acrylamide, BioRad) and 7 M urea (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... samples were combined and run on a 20% denaturing polyacrylamide gel (40% solution of 19:1 acrylamide:bis-acrylamide, BioRad) and 7 M urea (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-syntenin-1 (SCBT, sc-515538) and horseradish peroxidase-conjugated secondary goat anti-mouse (Bio-Rad, 170-6516), goat anti-rabbit (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... at a 1:10,000 dilution and we developed blots using the AP conjugate substrate kit (Bio-Rad; Hercules, USA). We used polyclonal GFP antibodies (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: Immunoreactive bands were visualized by incubation with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulins (1:5000, BioRad 170-6515) or goat anti-mouse immunoglobulins (1:5000 ...
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bioRxiv - Plant Biology 2021Quote: ... Four- to six-week-old expanded leaves of relevant Arabidopsis lines were bombarded with 1 nm gold particles (BioRad) coated with pB7WG2.0-mRFP ...