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Citations for Bio-Rad :
5451 - 5500 of 6619 citations for 6 4 METHYLPIPERAZIN 1 YL NICOTINONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... followed by streptavidin-alkaline phosphatase (1 µg/mL) and development with AP conjugate substrate kit (Bio-Rad). For analysis of intracellular cytokine production ...
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bioRxiv - Immunology 2021Quote: ... Expression of target and housekeeping genes (see Table 1) was analysed on CFXTM Manager (Bio-Rad Laboratories) using the “Gene Study” function ...
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bioRxiv - Microbiology 2021Quote: ... each cDNA was combined with segment-specific primers (Supplementary Table 1) and Precision melt supermix (Bio-Rad) and analysed by qPCR using a CFX384 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... and transferred for 1 hour at 15V using Trans-Blot® SD Semi-Dry Transfer Cell (BioRad). The PVDF membrane was blocked with 5% milk in Tris-buffered saline with 0.1% tween-20 (TBST ...
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bioRxiv - Developmental Biology 2022Quote: 1 µg of RNA was reverse transcribed using the iScript cDNA Synthesis Kit (Cat# 1708891, Bio-Rad) according to manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... cDNA was synthesized from 1 μg total RNA using iScript reverse transcription supermix (Bio-Rad, Hercules, CA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized from 1 ug of RNA using the iScript cDNA Synthesis Kit (BioRad #170-8891). Reverse Transcriptase Quantitative PCR (RT-qPCR ...
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bioRxiv - Cancer Biology 2022Quote: ... Each column then received 200 µL of ProteOn 1 M Ethanolamine hydrochloride pH 8.0 (BioRad, Cat. # 1762450) and was incubated for 4 hours to quench any remaining active site on the resin ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatant was mixed with sample loading buffer supplemented with 1% v/v 2-mercaptoethanol (BioRad, Cat #1610710). Sample was not heated to prevent aggregation of the GPCR ...
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bioRxiv - Genomics 2022Quote: ... 20 µg total protein extract per sample were boiled in final 1 x Laemmli Buffer (BioRad, 1610747) containing 10% 2-Mercaptoethanol (M6250 ...
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bioRxiv - Microbiology 2024Quote: ... Secondary antibodies used for immunoblots were StarBright Blue 520 goat anti-mouse (IgG; 1:10,000; Bio-Rad) and Horseradish peroxidase conjugated-goat anti-mouse (IgG ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were used: rat anti-myelin basic protein (MBP, 1:100, Bio-Rad antibodies MCA409S), mouse anti-myelin-associated glycoprotein (MAG ...
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bioRxiv - Molecular Biology 2022Quote: ... The gel was dried for 1 h at 80 °C on a Gel Dryer 583 (Bio-Rad) and visualized after appropriate exposure on a Phosphorimager (FLA-3000 Series ...
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bioRxiv - Neuroscience 2022Quote: ... and blocked for 1 h at room temperature in 5% skim milk (Catalog #170-6404, Bio-Rad). Primary antibody incubations were performed overnight at 4°C with antibodies diluted in TBS/0.1% Tween-20/5% BSA ...
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bioRxiv - Microbiology 2024Quote: ... The reaction mixtures (20 μl) consisted of 1 × iQ SYBR green Supermix (Bio-Rad, Hercules, CA, USA), 0.5 μM of each primer ...
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bioRxiv - Biochemistry 2024Quote: ... and the band intensities were quantified and analyzed using Quantity One 1-D software (Bio-Rad Laboratories).
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bioRxiv - Cell Biology 2024Quote: ... HRP signals were visualized by enhanced chemiluminescence (ECL) (Cytiva) (1:25000) and imaged with Chemidoc (Bio-Rad).
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bioRxiv - Molecular Biology 2024Quote: ... and transferred onto PVDF membranes using a wet blot system for 1 h at 100 V (Biorad). Membranes were blocked with BSA and incubated with primary antibody overnight at 4° C ...
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bioRxiv - Molecular Biology 2024Quote: ... This was followed by incubation with a secondary horseradish peroxidase-conjugated α-mouse antibody (BioRad – 1:2,000) for 1 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated in blocking buffer containing horseradish peroxidase-conjugated secondary antibodies at 1:10,000 dilution (Bio-Rad #172-1011 or 172-1019 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Primary antibodies were labeled with horseradish peroxidase-conjugated secondary antibody (1:5000) and ECL substrates (Bio-Rad). The densitometric intensities of the bands were quantified by using ImageJ software.
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bioRxiv - Evolutionary Biology 2024Quote: ... Synthesized cDNA was diluted 1:10 and 5 µl mixed with SsoAdvanced Universal Sybr Green supermix (Biorad). qPCR was performed using the Biorad CFX96 PCR machine ...
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bioRxiv - Immunology 2024Quote: ... 1:1200 diluted mouse anti-human HLA-E monoclonal primary antibody (clone MEM-E/02, Bio-Rad) and as secondary antibody goat anti-mouse polyclonal antibody conjugated with horse radish peroxidase (HRP ...
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bioRxiv - Microbiology 2024Quote: ... and human proinflammatory cytokines were quantified using the Bio-Plex Pro™ Human Inflammation Panel 1 (BioRad). Fluorescence was read using a MAGPIX System (Luminex ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... The gel was dried at 80°C for 1 h on a Gel Dryer 583 (Bio-Rad), exposed over night ...
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bioRxiv - Cell Biology 2023Quote: ... coli Rosetta (DE3) cells by electroporation (Bio-Rad 1 mm cuvette, 18,000 V/cm, 200V, 25 mF). Transformed E ...
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bioRxiv - Genomics 2023Quote: ... Blots were further washed thrice with 1× TBS-T and developed using Clarity Western ECL (BioRad, #1705060). Images were captured using the ImageQuant LAS 4000 gel documentation system ...
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bioRxiv - Microbiology 2023Quote: ... and transferred into PVDF membrane for 1 hrs at 16 V using the semi-dry method (BioRad). The membrane was blocked with 3% BSA / 5% skimmed milk in TBS ...
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bioRxiv - Biochemistry 2023Quote: 30% acrylamide:bis-acrylamide (99:1 ratio) solution was prepared by mixing 7.4 mL of 40% acrylamide (BioRad), 1.5 mL of 2% Bis-Acrylamide (Biorad ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg total RNA was subjected to reverse transcription with the iScript cDNA synthesis kit (Bio-Rad). In case of RNA isolated with TRIzol ...
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bioRxiv - Pathology 2023Quote: ... at a dilution of 1:100 and fibrinogen using the monoclonal anti-fibrinogen antibody (#4440-8004, BioRad) at a dilution of 1:200 incubating over night at 4° C in a humidified chamber ...
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bioRxiv - Genomics 2023Quote: ... the blots were incubated in and hFAB™ Rhodamine Anti-Actin (BioRad #12004163 at 1:2000 dilution). All experiments were performed in triplicate.
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bioRxiv - Neuroscience 2023Quote: 1 to 0.5 μg of total RNA was reverse transcribed using iScript cDNA Synthesis Kit (Bio-Rad). A volume corresponding to 5 ng of initial RNA was employed for each real-time PCR reaction using PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... mixed plasmid DNA (50 μg total) was coated on 1 μM-diameter gold particles (Bio-Rad, 1652263) in 0.5 mM spermidine ...
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bioRxiv - Immunology 2023Quote: ... RNA (0.5 to 1 μg) was reverse transcribed to cDNA using iScript cDNA Synthesis Kit (Bio-Rad), and real-time PCR reactions were performed as described previously10 ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1 µg RNA was used to synthesize cDNA using the iSCRIPT cDNA kit (Biorad, Hercules, USA) according the manufacturers description.
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bioRxiv - Molecular Biology 2023Quote: 1 μg Turbo DNase-treated RNA was reverse transcribed with the iScript cDNA synthesis kit (BioRad, 1708890), treated with 0.5 μL RNase cocktail (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was synthesized from 1 µg RNA using iScript gDNA Clear cDNA Synthesis Kit (BIORAD, Hercules, CA), according to manufacturer’s instructions and the cDNA was diluted to 5 ng/µl.
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bioRxiv - Cell Biology 2023Quote: ... cDNA was synthesized from 1 μg of total RNA using the iScript cDNA synthesis kit (Bio-Rad). qPCR was performed using the LightCycler 480 (Roche ...
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bioRxiv - Microbiology 2023Quote: ... destained in 7.5% acetic acid for 1 minute and imaged using a ChemiDoc imaging system (Bio-Rad). For western blotting ...
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bioRxiv - Biochemistry 2022Quote: ... stock solution of 40% neutral acrylamide and N,N’-methylenebisacrylamide in a 19:1 ratio (Bio-Rad) were mixed with a stock solution of 40% positively charged (3-acrylamidopropyl)-trimethylammonium chloride (75% wt ...
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bioRxiv - Cell Biology 2023Quote: ... RNA (1 μg) was then converted to cDNA using an iScript cDNA Synthesis Kit (Bio-Rad: #1708891BUN), which was then analyzed on a BioRad CFX96 Real Time Thermocycler using 2X SYBR green qPCR master mix (Life Technologies ...
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bioRxiv - Genetics 2023Quote: ... and cDNA was prepared from 1 μg of RNA using the iScript cDNA synthesis kit (Bio-Rad), both according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: ... and resolved in a 1% LE agarose gel in 0.5x TBE using a CHEF-DRII instrument (BioRad) using the following settings ...
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bioRxiv - Immunology 2023Quote: ... cDNA was synthesized from 1 μg total RNA using iScript reverse transcription supermix (Bio-Rad, Hercules, CA) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... Detergent removal was initiated 1 hour after mixing all ingredients by adding 200 mg of BioBeads (BioRad) and mixing at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted 1:1000 in PBS-T containing 5% blotting-grade blocker (Bio-Rad, #1706404). Membranes were incubated overnight (≈16 h) ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA reverse-transcribed from 1 µg of this RNA using iScript Reverse Transcription Superrmix (Bio-Rad, 1708840) was used as a template for performing qPCR using TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific ...
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... 1 μg of RNA was reverse-transcribed to cDNA (Bio-Rad Real-time PCR system, Hercules, CA). Stat3 deletion or differential gene expression between experimental groups was confirmed by performing qRT-PCR in a CFX real-time PCR machine (Bio-Rad ...
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bioRxiv - Physiology 2023Quote: ... 1 µg of total RNA was converted into cDNA using the iScript Reverse Transcription Supermix (Bio-Rad). RT-qPCR was performed using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad ...