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Citations for Bio-Rad :
5001 - 5050 of 5608 citations for Mouse Leucine Rich Pentatricopeptide Repeat Containing LRPPRC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... cDNA was produced using the High Capacity RNA-to-cDNA Kit (Applied Biosystem) and the cDNA was pre-amplified with SsoAdvanced PreAmp Supermix (Bio-Rad) using primers from Integrated DNA Technologies or Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... 0.5 μg of total RNA sample (100 ng/μl) was retrotranscribed in stable cDNA with iScriptTM Advanced cDNA Synthesis Kit (Bio-Rad). Genes of interest were amplified with Itaq UniversSYBR Green (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Up to 5 μg of total RNA was reverse transcribed into cDNA using iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR (Bio-Rad). Quantitative real time-PCR was performed using GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 ng of total RNA was used to generate cDNA via the iScript Advance cDNA Kit for RT-qPCR (BioRAD #1725037), for each sample including no reverse transcriptase and no template controls following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 ng of total RNA was used to generate cDNA via the iScript Advance cDNA Kit for RT-qPCR (BioRAD #1725037), for each sample including no reverse transcriptase and no template controls following the manufacturer’s protocol ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: Membrane and cytosolic proteins fractions from MS were extracted using ReadyPrep™ Protein Extraction Kit (Membrane I) (Bio-Rad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The cDNA samples were used in a qRT-PCR experiment employing the iQ SYBR Green Supermix Kit (Bio-Rad, Munich, Germany). PCR was conducted in a CFX96 RT-PCR Detection System (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: Agarose plug embedded nuclei were Proteinase K treated for two days followed by RNAse treatment (Biorad CHEF Genomic DNA Plug Kit). DNA was recovered from agarose plugs according to IrysPrep™ Plug Lysis Long DNA Isolation guidelines (BioNano Genomics) ...
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bioRxiv - Immunology 2020Quote: ... A 1 µg sample of total RNA was reverse transcribed to complementary DNA using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... Concentration of protein lysates was determined using either the Pierce BCA protein assay kit (Pierce) or the DC Protein Assay (Bio-Rad) following the manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... An amount of 500-1000 ng of purified total RNA was then subjected to reverse transcription using the iScript cDNA synthesis kit (Bio-Rad), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... 800 ng of purified RNA was used as a template to generate cDNA with the iScript cDNA Synthesis Kit (Bio-Rad). End-point PCR was then conducted using primer sets EH13/EH14 and EH1/EH2 ...
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bioRxiv - Neuroscience 2019Quote: ... total protein concentration of the supernatant was assayed by using Bio-Rad protein assay kit (Bio-Rad, Marnes-la-Coquette, France). Equal amounts of each protein sample were separated on NuPAGE Bis-Tris or Tris-Acetate gels and transferred to nitrocellulose or PVDF membranes ...
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bioRxiv - Physiology 2019Quote: ... Antennal cDNA was synthesized from 1 µg of antennal total RNA from each species using iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions (Clontech ...
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bioRxiv - Microbiology 2019Quote: ... 39) and 1 µg of total RNA was reverse transcribed to cDNA using the iScript Select cDNA Synthesis Kit (Bio-Rad) with random hexamers ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: Quantitative real-time (qRT)-PCR was performed using the iCycler-iQ system and the iQ SYBR Green Supermix Kit from Bio-Rad. The primer pairs used to amplify the target genes were designed using the Primer3 software (http://bioinfo.ut.ee/primer3/ ...
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bioRxiv - Plant Biology 2019Quote: ... and the Takyon No ROX SYBR 2X MasterMix blue dTTP qPCR Kit (both Eurogentech) for quantitative real time PCR using a CFX96 detection system (Bio-Rad). The previously described reference genes Protein Phosphatase 2A (PP2A ...
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bioRxiv - Cell Biology 2019Quote: ... RNA concentration and quality were determined using Nanodrop (PeqLab) and cDNA was synthesized with the iScript cDNA synthesis kit (Bio-Rad) following manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... We then performed quantitative PCR (qPCR) using 2 ng of cDNA for each reaction using the iTaq Universal SYBR Green Supermix kit (Bio-Rad) on a Bio-Rad CFX384 thermocycler ...
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Transcriptomic and morphological response of SIM-A9 mouse microglia to carbon nanotube neuro-sensorsbioRxiv - Bioengineering 2020Quote: Total RNA from SIM-A9 cells with varying treatment conditions was reverse transcribed to cDNA libraries using the iScript cDNA Synthesis Kit (Bio-Rad) with a 1 μg RNA input ...
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bioRxiv - Physiology 2019Quote: ... The cDNA synthesis was performed with 1μg of total RNA using the iScript cDNA synthesis kit (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Bioengineering 2021Quote: ... The HRP conjugated secondary antibodies were visualized by using an enhanced chemiluminescence detection kit and ChemiDoc Touch Imaging System (Image Lab, Bio-Rad).
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bioRxiv - Evolutionary Biology 2020Quote: ... DNase-treated RNA was used to perform cDNA synthesis in a final volume of 20 μl using the iScript cDNA synthesis kit (Biorad, USA). The reaction was performed at 46°C for 20 min ...
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bioRxiv - Cancer Biology 2020Quote: ... The chemiluminescence of the membranes was developed using Super Signal Femto Substrate Kit (Pierce) and developed using ChemiDoc Touch Imager (Bio-Rad). Primary antibodies and conditions used are as follows ...
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bioRxiv - Genomics 2021Quote: ... qRT-PCR was performed using the TaqMan Fast Advanced Cells-to-CT kit with the FX96 Real-Time PCR Detection System (Bio-Rad) with the TaqMan probes listed in table S15 ...
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bioRxiv - Immunology 2021Quote: ... were detected by staining membranes with anti-mouse IFNγ biotin (1mg/mL) (R46A2) followed by streptavidin-Alkaline Phosphatase (1mg/mL) and development with AP conjugate substrate kit (BioRad, UK). Spots were enumerated using an AID ELISpot reader and software (AID).
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bioRxiv - Systems Biology 2021Quote: ... Transfer was done into PVDF membranes using a Trans-Blot Turbo Transfer System and Trans-Blot Turbo RTA Transfer Kit (BioRad, #1704273). After 1 h of blocking in 5% milk ...
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bioRxiv - Neuroscience 2020Quote: ... Five hundred ng of purified RNA were used for cDNA synthesis using an iScript cDNA synthesis kit (Bio-Rad, Irvine, CA), and real-time quantitative PCR (qPCR ...
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bioRxiv - Bioengineering 2021Quote: ... A preamplification step that amplifies the amount of cDNA available for downstream RT-qPCR analysis was conducted using SsoAdvanced PreAmp Kit (BioRad, 1725160). RT-qPCR was performed using TaqMan Gene expression assays (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Cell lysates were boiled for 10 min and quantified with the DC protein assay kit following the microplate protocol provided by the company (Bio-Rad). Whole cell lysates (15-40μg protein ...
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bioRxiv - Biochemistry 2021Quote: ... RQN > 9) were prepared using the NEBNext Ultra II Directional RNA Library Kit for Illumina with NEBNext rRNA depletion (Bio-Rad). Protocols were followed as per the manufacturer’s guidelines except that 40X adaptor dilutions were utilized ...
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bioRxiv - Biochemistry 2020Quote: ... the protein concentration was determined using the BioRad DC microplate assay protocol (BioRad DC Protein Assay Kit, BioRad, Hercules, California #5000112). Protein Standards and samples were run on NuPAGE 10% Bis-Tris Gels (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... N2a cells were harvested at the same time points for reverse-transcriptase (RT) PCR generation using iScript cDNA Synthesis kit (Bio-Rad), and quantitative PCR (QPCR ...
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bioRxiv - Cancer Biology 2020Quote: RNA was isolated and measured as detailed above and then converted to cDNA using iScript cDNA Synthesis Kit (Bio-Rad #1708891). Quantitative RT-PCR was carried out with TaqMan Gene Expression Assay reagents ...
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bioRxiv - Cancer Biology 2020Quote: ... Revelation was performed using secondary antibodies conjugated to horse-radish peroxidase (GE-Healthcare) diluted 1:2,000 in saturation solution and Opti-4CN™ colorimetric kit (Bio-Rad). ImageJ software was used to measure the relative intensity of protein bands.
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription of 1 μg of RNA in a total volume of 20 μl was performed with iScript cDNA Synthesis Kit (Bio-Rad) prior to real time PCR (iCycler ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative real time PCR was performed using the iQTM SYBR Green Supermix PCR kit with the iCycler apparatus system (Bio-Rad). GAPDH was used as the invariant housekeeping gene internal control.
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bioRxiv - Physiology 2020Quote: ... We synthesized a total of 500 ng (pituitary) and 1 μg (gonad) of RNA into cDNA using the iScript cDNA synthesis kit (Bio-Rad) in 20 μl reactions according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 µg of each RNA sample was used in a 10-µL reverse transcription reaction set up using the iScript Select cDNA synthesis kit (Bio-Rad), together with gene-specific reverse primers (Table S2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... We performed cDNA synthesis with 0.5-1 μg of total RNA using the iScript Select cDNA Synthesis Kit (Bio-Rad Laboratories). We performed Real Time quantitative PCR (RT-qPCR ...
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bioRxiv - Systems Biology 2020Quote: ... and used 500 ng - 1 ug RNA to make cDNA using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA). 0.1 of cDNA (i.e ...
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bioRxiv - Plant Biology 2020Quote: ... and the Takyon No ROX SYBR 2X MasterMix Blue dTTP qPCR Kit (both Eurogentech) for quantitative real-time PCR using a CFX96 detection system (Bio-Rad). Transcript data was normalized to UBQ10 ...
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bioRxiv - Plant Biology 2021Quote: ... ∼1 ug of RNA from resulting extraction was used as template in standard cDNA synthesis reaction (BioRad iScript cDNA Synthesis Kit). 200 ng of resulting cDNA was used in qRT-PCR reactions to quantify POL expression levels (PowerUp SYBR Green 5x Master Mix-Thermo) ...
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bioRxiv - Microbiology 2021Quote: ... RNA samples were stored at −80°C until cDNA synthesis using the iScript™ gDNA Clear cDNA Synthesis Kit according to the manufacture’s guidelines (BIO-RAD). A qPCR-based assay was used to detect and quantify PfCSP transcripts (Kefi et al. ...
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bioRxiv - Genomics 2021Quote: ... 30-40 ng of RNA was used to make cDNA using the iScript Adv cDNA Syn Kit (Bio-Rad life Sciences). The RNA was diluted 1:50 in water for ddPCR analysis ...
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bioRxiv - Genetics 2021Quote: ... 0.4 μM or 3.2 μM of SAM-3H3 were used and the reaction was cleaned with either a PCR purification kit or Bio-Spin P30 columns (Bio-rad). The reactions were incubated for 2 hrs at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... Immunoreactive bands were detected using an ECL detection kit (ATTO, EzWestLumi plus, WSE-7120) with a ChemiDoc imaging system (BIO-RAD).
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bioRxiv - Microbiology 2021Quote: ... Additional one-ml samples were used for total protein determination using the Bio-rad Protein Assay kit (Bio-rad, CA, USA). β-galactosidase assays were performed as described previously (38) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... SYBR Green qPCR was performed using a ViiA7 (A&B Biosystems, Waltham, MA) with iTaq Universal SYBR Green One Step Kit (BioRad, #1725151) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... The blots were developed using enhanced chemiluminescence using Cynagen Westar Supernova detection kit and imaged using ChemiDocTM system from Bio-Rad. The blots were then stripped and re-probed for total proteins ...