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Citations for Bio-Rad :
4951 - 5000 of 5608 citations for Mouse Leucine Rich Pentatricopeptide Repeat Containing LRPPRC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred to PVDF membranes using a Trans-Blot Turbo RTA Mini 0.2 µm PVDF Transfer Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... RNA from vehicle (DMSO)-treated and 1 μM CHIR-treated cells was extracted using an Aurum Total RNA Kit (BioRad) and stored at -80°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was prepared using iScriptTM cDNA synthesis kit and qRT-PCR was performed using a CFX96 Touch Real-Time PCR Detection System (BioRad). Data were analyzed using the following equations ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA (1μg) was reverse transcribed into complementary DNA using an iScript Reverse Transcription Supermix kit (Bio-Rad, Hercules, CA, USA). The relative levels of gene transcripts compared with the control gene 18S were determined by quantitative real-time PCR using SYBR Green PCR Supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... TX) and 750ng of RNA was reverse transcribed to generate cDNAs using iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). Relative transcript levels were analyzed by real-time qPCR using SYBR Green (SsoAdvanced Universal SYBR Green Supermix ...
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bioRxiv - Cancer Biology 2023Quote: ... and the product band was excised and extracted using the Freeze and Squeeze Kit according to the manufacturer’s instructions (Bio-Rad). Gel-purified amplicons were quantified with a dsDNA HS Assay kit on a Qubit fluorometer (ThermoFisher Scientific) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... aliquots constituting 250 ng of RNA were diluted with nuclease-free water to a volume of 15 µL and processed using the iScriptTM cDNA Synthesis Kit (BioRad Laboratories Inc. ...
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bioRxiv - Genomics 2023Quote: ... Two μg of total RNA each was used for cDNA synthesis using iScript™ cDNA Synthesis Kit (Bio-Rad, USA). Oligonucleotide primers used for RT-PCR for each gene are listed in Supplementary Table S4 ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the supernatant was measured using the modified Lowry assay (DCTM Protein Assay Kit, BIO-RAD, 5000111). The same amount of total protein was mixed with Laemmli sample buffer (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... 500 ng RNA was reverse transcribed using HiScript III 1st Strand cDNA Synthesis Kit followed by qPCR reactions using SYBR Green master mix and analyzed by Biorad CFX Touch Real-Time PCR Detection System (SIA-PCR008) ...
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bioRxiv - Biophysics 2023Quote: ... proteins were transferred to PVDF membranes using a Trans-Blot Turbo RTA Mini 0.2 μm PVDF Transfer Kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Membrane strips were finally washed 6x for 3mins in 1X TBST before being developed with the ECL Clarity Kit (BioRad) and imaged on ChemiDoc Imaging system (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... High integrity RNA (RIN > 7) was used to synthesize cDNA with iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad). PCR primers for human B-ACTIN ...
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bioRxiv - Microbiology 2024Quote: TNF-α and IL-10 were quantified in the collected supernatants using Bio-Plex Pro Human Luminex kit (Bio-Rad) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... and reverse transcribed by Maxima first strand cDNA synthesis kit (Thermo Fischer) using Bio-Rad CFX Maestro 1.1 software (Bio-Rad). The primers used are listed in Supplementary table 6.
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bioRxiv - Cell Biology 2024Quote: ... submerged with ECL solution (ECL Prime Kit; Amersham, UK) and chemiluminescence signals were detected using the ChemiDoc system from BioRad. The following antibodies were used ...
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bioRxiv - Molecular Biology 2024Quote: HepG2 protein lysate was separated by SDS-Page (TGX Stain-FreeTM FastCastTM Acrylamide Kit, Bio-Rad Laboratories, Inc., Hercules, US) and blotted on nitrocellulose membranes (Trans-Blot® TurboTM ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was then synthesized from 1 µg total RNA (measured by spectrophotometer) with the iScriptTM cDNA synthesis kit (Bio-RAD) according to the company’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... were transferred to a 0.45μM nitrocellulose membrane (Trans-Blot Turbo RTA Transfer kit nitrocellulose midi #1704271) using Trans-Blot Turbo Transfer System (Bio-Rad #1704150 ...
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bioRxiv - Molecular Biology 2024Quote: ... The washed membrane was detected using the Clarity Western ECL kit following manufacturer protocol and imaged using a ChemiDoc XRS+ apparatus (BioRad). Pixel density for each dot was quantified using the volume tool in ImageLabTM software (BioRad).
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bioRxiv - Pathology 2024Quote: ... We used 200ng of total RNA to synthesize single-stranded cDNA using iScript cDNA synthesis kit based on manufacturer’s directions (Bio-Rad). cDNA was then used for qRT-PCR analysis using SYBR Green qPCR master mix (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... Detection was performed using ECL Advance Western Blotting Detection Kit (Amersham) and the chemiluminescence was detected using an ChemiDoc Imaging System (BioRad).
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bioRxiv - Molecular Biology 2024Quote: ... 1µg of DNase I treated RNA was converted into cDNA using an iScript cDNA synthesis kit (BIO-RAD, Cat:1708891) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2024Quote: ... Total reaction volume was 10 µl and contained 5 µl of iTaq Universal SYBR green one-step kit (Bio-Rad), 300 nM of forward and reverse primers (Table S5 ...
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bioRxiv - Pathology 2024Quote: ... Hrd1 and GAPDH was quantified through one-step RT-qPCR using iTaq Universal SYBR green one-step kit (Bio-Rad) with the corresponding primiers (Table S5 ...
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bioRxiv - Genomics 2024Quote: ... 500 ng of RNA was further reverse transcribed to cDNA using the iScript cDNA synthesis kit (Bio-Rad, CA, USA). Semi-quantitative PCR was carried out using specific primers for the following genes ...
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bioRxiv - Immunology 2024Quote: The concentration of total protein in each processed sample was quantified using the DC Protein Assay Kit II (Bio-Rad) with a standard curve (R2 > 0.99 ...
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bioRxiv - Microbiology 2024Quote: Cytokine/chemokine expression levels were further confirmed using the Bio-Plex Multi-Plex Assay Kit (Bio-Rad Laboratories, Hercules, CA). Supernatants from organ homogenates were used to determine presence of 22 proteins including eotaxin ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA samples were obtained from 1 µg RNA using the iScriptTM cDNA synthesis kit (Bio-Rad, Cat no. 170-8891) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Gene-specific cDNA synthesis was carried out with 2 µg of mtRNA using the iScript Select cDNA Synthesis Kit (BioRad) and oligo 2278 (see Supplementary Table ST2) ...
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bioRxiv - Systems Biology 2024Quote: ... 1 µg of total RNA were processed using the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad; www.bio-rad.com), to achieve genomic DNA digestion and first-strand cDNA synthesis ...
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bioRxiv - Neuroscience 2024Quote: ... and the RNA was reverse transcribed into cDNA using the iScript™ gDNA Clear cDNA Synthesis Kit (1725035, Bio-Rad), as per the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of total RNA was converted to cDNA using iScript advanced cDNA synthesis kit for RT-qPCR (Cat# 1725037, Biorad) according to the instructions of the manufacturer.
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bioRxiv - Immunology 2024Quote: ... cDNA was made from equivalent starting amounts for each sample and synthesized using the iScript cDNA Synthesis Kit (Bio-Rad). Total cDNA was diluted 1:10 in water and amplified by real-time polymerase chain reaction using a BioRad CFX-96 iCycler and SYBER Green Supermix ...
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bioRxiv - Plant Biology 2020Quote: ... One microgram of total RNA was reverse transcribed to complementary DNA (cDNA) using the iScript cDNA Synthesis Kit (Biorad, Hercules, California) in a 20 μL reaction volume ...
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bioRxiv - Cell Biology 2020Quote: ... and detected using the antibodies listed in Supplementary File 3 with either SuperSignal West Dura or SuperSignal Femto kits (Pierce, Waltham, MA) and a ChemiDoc XRS imaging system (Bio-Rad). Band intensities were quantified using ImageLab software (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... and NuPAGE sample buffer and reducing buffer (LifeTechnologies) were added after measuring and adjusting the samples for protein concentration (DC protein assay kit II, Bio-Rad). 2-20 μg protein per sample were separated for 2-4 h at 60 – 100 V using 8 % 26 well NuPAGE Bis-Tris Midi gels in MOPS or MES buffer (LifeTechnologies ...
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bioRxiv - Cell Biology 2019Quote: RNA was extracted from 40 w1118 or de2f1b 80-85 hr AEL salivary glands using the Aurum Total RNA Mini Kit (Bio-Rad) with treatment of DNase I to remove genomic DNA contamination ...
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bioRxiv - Genetics 2021Quote: ... qPCR was carried out using BioMaster HS-qPCR SYBR Blue (2×) reagent kit (Biolabmix; https://biolabmix.ru/en/) and CFX96™ Real-Time PCR Detection System (Bio-Rad). We used the following thermal cycling conditions ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 µg of total RNA and oligo (dT) primers were reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). RT-PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... was performed as a two-step RT-qPCR by first synthesizing oligo(dT) primed cDNA using the iScript™ Select cDNA Synthesis Kit (Biorad), then running ddPCR via the standard EvaGreen program on a Biorad QX200 ddPCR system and analyzing in Quantasoft software (see Table S2 for primer sequences).
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed again 3 times 10min in 1X TBS-T and developed using the Clarify Western ECL substrate kit (Bio-Rad) or SuperSignal West Femto (ThermoFisher Scientific) ...
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bioRxiv - Systems Biology 2022Quote: ... Drugs were then added to the plates and protein was harvested after 2 hours using the Bio-Plex Pro Cell Signaling Reagent Kit (BioRad 171304006M) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... and TNF-α were quantified using real-time PCR and the SsoAdvanced Universal SYBR Green Supermix Kit (BIO-RAD, Hercules, CA). β-actin was used as an internal control ...
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bioRxiv - Neuroscience 2021Quote: ... analysis was performed through reverse transcription on 0.5-1 μg of total RNA using the iScript cDNA synthesis kit according to the manufacturer’s instructions (Bio-Rad, Hercules, CA). The cDNA was diluted 1:20 in nuclease-free water ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA (280 ng per sample) was used to generate cDNA using the iScript gDNA Clear cDNA Synthesis Kit (Bio-Rad). The cDNA was used as a template for qRT-PCR with SsoFast EvaGreen Supermix (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 μL of DNase-treated samples were reverse transcribed in a final volume of 10 μL using the iScript cDNA synthesis kit (Bio-Rad) following the manufacturer’s protocol and diluted 5-fold in nuclease-free water before qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... the volume of BioID lysis buffer added was based on protein concentration determination using the DC Protein Assay Kit from Bio-Rad on the 10% aliquot samples lysed in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative real-time PCR expression analysis of selected candidates was performed using cDNAs produced from the iScript cDNA Synthesis Kit (Bio-Rad) in a LightCycler 480 system (Roche ...
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bioRxiv - Immunology 2020Quote: ... 18.10 is a murine IgG1 anti-human HVEM mAb and was produced as ascites and purified by protein A binding and elution with the Affi-gel Protein A MAPS II Kit (Bio-rad). The mouse IgG1 isotype control (clone MOPC-21 clone) ...