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Citations for New England Biolabs :
51 - 100 of 615 citations for Universal TT epitope P2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... using Luna Universal qPCR master mix (NEB, Ipswich, MA). Primer sequences can be found in Table S17 ...
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A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testingbioRxiv - Molecular Biology 2021Quote: ... or Luna Universal One-Step RT-qPCR Kit (NEB) and 1.5 μl of reference primer/probe sets CDC-N1 (IDT 10006713 ...
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bioRxiv - Immunology 2022Quote: ... and the Luna Universal qPCR Master Mix (NEB, M3003). Final quantification was performed using the ΔΔCT method.
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bioRxiv - Developmental Biology 2019Quote: ... using Luna® Universal qPCR Master Mix (NEB #M3003S) following instructions in the reagent manual ...
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bioRxiv - Genetics 2021Quote: ... 30 blocked adaptor (Universal miRNA cloning linker, NEB S1315S) with 250 units of T4 RNA ligase truncated KQ (NEB M0373S) ...
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bioRxiv - Microbiology 2023Quote: ... including Luna® Universal qPCR Master Mix (NEB, UK), one primer pair (cDNA template ...
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bioRxiv - Molecular Biology 2023Quote: Luna Universal qPCR Master Mix (New England Biolabs, USA) and other reaction components were thawed at room temperature and gently vortexed ...
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bioRxiv - Plant Biology 2023Quote: ... Luna® Universal qPCR Master Mix (NEB, Cat. M3003L) and qPCR machine (Biorad ...
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bioRxiv - Cell Biology 2023Quote: ... or Luna Universal qPCR Master Mix (New England Biolabs) according to the manufacturer’ s instructions.
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bioRxiv - Microbiology 2023Quote: ... the Luna® Universal qPCR Master Mix from NEB was used according to the manufacturer’s instructions with a total reaction volume of 10 μL ...
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bioRxiv - Immunology 2023Quote: ... using the Luna Universal qPCR Master Mix (NEB, M3003), and included initial enzyme activation for 3 minutes at 95°C ...
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bioRxiv - Systems Biology 2023Quote: ... using the NEBNext Universal i5 primer (New England Biolabs) and the N7 indices from the Nextera XT Index Kit v2 Set A (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... with the Universal miRNA Cloning Linker (New England BioLabs) as the 3’ adaptor and in-house designed indexed primers ...
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bioRxiv - Plant Biology 2024Quote: ... with Luna Universal qPCR Master Mix (New England Biolabs). The cycling parameters were 1 min at 95°C ...
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bioRxiv - Cancer Biology 2024Quote: ... using the Luna Universal qPCR Master Mix (NEB, M3003), and included initial enzyme activation for 3 minutes at 95 °C ...
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bioRxiv - Microbiology 2019Quote: ... Fragments ranging from 500-750 bp and corresponding to sequences upstream (P1 and P2) and downstream (P3 and P4) of the gene to be deleted were amplified using Phusion polymerase (New England Biolabs, Ipswich, MA). Primers P2 and P3 were designed with 5’ sequences with reverse complementarity to each others’ 3’ proximal sequence ...
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bioRxiv - Genomics 2020Quote: ... These were ligated to modified Illumina P1 and P2 adapters overnight at 16 °C with 1,000 units of T4 ligase (New England Biolabs, Beverly, MA, USA), followed by a 10-minute heat-deactivation step at 65 °C ...
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bioRxiv - Immunology 2022Quote: ... and Luna® Universal RT-PCR Kit (New England Biolabs) in a StepOnePlus apparatus (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... The Luna universal qPCR Master mix (New England BioLabs, M3003L) was prepared according to the manufacturer’s instructions with a reaction volume of 10µL ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was mixed with Luna Universal qPCR Master Mix (NEB). RpL32 (rp49 ...
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bioRxiv - Microbiology 2020Quote: ... and 2x Luna universal qPCR master mix (New England Biolabs) in a 25μl total volume ...
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bioRxiv - Cell Biology 2021Quote: ... Universal PCR primers and Index (X) Primer (New England BioLabs). At last ...
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bioRxiv - Genetics 2022Quote: ... pipientis infection status by qPCR (NEB Luna Universal qPCR kit) using primer sets for wsp and arm (S1 Table) ...
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bioRxiv - Immunology 2022Quote: ... RT-qPCR was performed with Luna Universal qPCR reagent (NEB). eMLV env was detected with 5’-AGGCTGTTCCAGAGATTGTG-3’ and 5’-TTCTGGACCACCACATGAC-3’ and 18S rRNA was detected with 5’-GTAACCCGTTGAACCCCATT-3’ and 5’-CCATCCAATCGGTAGTAGCG-3’ on Roche LightCycler 480 II (104,105).
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bioRxiv - Genetics 2022Quote: ... Purified RNA was then ligated with universal miRNA adaptor (NEB). Excessive adaptors were removed by running the ligation product in 15% polyacrylamide TBE-urea gel ...
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bioRxiv - Biochemistry 2020Quote: ... Luna Universal One-Step RT-qPCR kit (New England Biolabs) was used following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... or with Luna Universal qPCR Master Mix (New England Biolabs) on a CFX Connect Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Molecular Biology 2022Quote: ... and ligated to either the Universal miRNA Cloning Linker (NEB; 25S rRNA samples and some 18S rRNA samples ...
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bioRxiv - Microbiology 2022Quote: ... Luna® Universal One-Step RT-qPCR Kit from NEB was used for RT-qPCR reactions ...
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bioRxiv - Bioengineering 2022Quote: ... The Luna Universal qPCR Master Mix (New England Biolabs, M3003X) was used to monitor cDNA amplification ...
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bioRxiv - Microbiology 2023Quote: ... using Luna Universal qPCR Master Mix (New England Biolabs, USA) and gene specific primers (Supp Table 4) ...
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bioRxiv - Cancer Biology 2023Quote: ... with Luna Universal qPCR Master Mix (M3003X; New England Biolabs). The qPCR primer sequences for the detection of PU.1 binding region were as follows (5’-3’) ...
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bioRxiv - Genetics 2023Quote: ... The Luna Universal Probe One-Step RT-qPCR kit (NEB) following the manufacturer’s protocol was used for the quantification of IBV derived RNA using IBV specific primers (forward 5′-GCTTTTGAGCCTAGCGTT-3′ and reverse 5′-GCCATGTTGTCACTGTCTATTG-3′ ...
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bioRxiv - Immunology 2023Quote: ... qPCR was performed using Luna universal QPCR mix (NEB # M3003) in the CFX96 Real-Time PCR system with a C1000 Thermal Cycler (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... with Luna Universal qPCR Master Mix (M3003X; New England Biolabs). The qPCR primer sequences were as follows (5’-3’) ...
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bioRxiv - Developmental Biology 2024Quote: ... qPCR was performed using Luna Universal qPCR Master Mix (NEB) with Bio-Rad iQ5 multicolor real-time PCR detection system ...
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bioRxiv - Plant Biology 2024Quote: ... the Luna® Universal qPCR Master Mix (New England Biolabs) was used ...
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bioRxiv - Molecular Biology 2024Quote: ... using the Luna Universal One-Step RT-qPCR Kit (NEB), and then analyzed with QuantStudio Design & Analysis Software v.1.5.1 ...
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bioRxiv - Microbiology 2023Quote: ... and Luna Universal qPCR Master Mix kit (New England Biolabs). Reactions were performed as technical replicates within each of three biological replicates ...
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bioRxiv - Cell Biology 2020Quote: ... addition of a N-terminal Hemagglutinin (HA) epitope was done using Q5 Site-Directed Mutagenesis Kit (New England Biolabs) following manufacturer recommendations.
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bioRxiv - Biochemistry 2023Quote: ... the HA-epitope was replaced by a 6xHis tag using the Q5 Site Directed Mutagenesis Kit (New England Biolabs) to generate Ptch1 KR-His ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR experiments were performed using SsoAdvanced Universal SYBR Green Supermix (NEB) on the Quantstudio5 (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 μL of Universal PCR Primer (25 mmol) (NEB, Ipswich, USA), and 1 μL of Index (X ...
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bioRxiv - Molecular Biology 2019Quote: ... with Luna Universal One-Step RT-qPCR reagents (New England Biolabs). 50 ng total RNA and 250 nM primers were added in each reaction ...
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bioRxiv - Microbiology 2021Quote: ... qPCRs were carried out using the Luna Universal qPCR MasterMix (NEB) with the primers listed in Supplementary Table 3 and measured on an Applied Biosystems 7500 Real-Time PCR system ...
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bioRxiv - Synthetic Biology 2021Quote: ... RT-qPCR (Luna Universal qPCR Master Mix; New England BioLabs; M3003L) was performed in 384 well plates (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... The Luna Universal One-Step RT-qPCR Kit (New England Biolabs) was used ...
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bioRxiv - Genomics 2022Quote: ... and Luna Universal qPCR Master Mix at 1X concentration (NEB, M3003L) in total reaction volume of 20 µL ...
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bioRxiv - Microbiology 2022Quote: ... Luna® Universal qPCR Master mix (New England Biolabs, cat# M3003L) was employed for RT-qPCR according to manufacturer’s recommendations (Initial denaturation 95°C ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was done using the Luna Universal qPCR Master Mix (NEB) and run on a CFX connect qPCR Detection System (BioRad) ...