Labshake search
Citations for New England Biolabs :
301 - 350 of 615 citations for Universal TT epitope P2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... RT-qPCR was performed using Luna® Universal One-Step RT-qPCR Kit (New England Biolabs), following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... Strain specific abundance was assessed with the Luna® Universal qPCR Master Mix (New England Biolabs) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... diluted in pure water and quantified with the Luna® Universal qPCR Master mix from NEB as previously described.
-
bioRxiv - Neuroscience 2022Quote: ... RT-qPCR reactions were performed using Luna® Universal qPCR Master Mix (New England Biolabs #M3003). Primers for qPCR reactions are shown in Table 1 ...
-
bioRxiv - Plant Biology 2022Quote: ... qPCR was performed using the Luna Universal qPCR Master Mix (New England Biolabs Japan, Tokyo, Japan) on a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2022Quote: ... RNA was reverse-transcribed and PCR amplified using Luna Universal One-Step RT-PCR Kit (NEB). SARS-CoV-2 replication was assessed by using primers specific to the N mRNA (Forward 5’-CTCTTGTAGATCTGTTCTCTAAACGAAC-3’ ...
-
bioRxiv - Microbiology 2023Quote: ... 200 μM dNTPs and 2500 nM NEBNext Universal and Index Primer each (Primer Set 1, NEB). PCR products were purified by native PAGE and ethanol-precipitated ...
-
bioRxiv - Physiology 2023Quote: ... using Luna Universal qPCR master mix for Sybr Green primers (New England Biolabs, Cat. No. M3003X). All primer sequences are listed in Supplementary Table 1 ...
-
bioRxiv - Immunology 2023Quote: Reverse transcriptase qPCR was performed using Luna Universal Probe One-Step RT-qPCR Kit (NEB, # E3006L). The following predesigned primers and probe sets for each target and the housekeeping gene GAPDH were purchased from IDT:
-
bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was performed with the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) according to manufacturer’s instruction on an iQ5 Real-Time PCR System (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The samples were prepared using a Luna Universal One-Step RT-qPCR Kit (New England Biolabs). Quantitative RT-PCR was performed with an Mx3000P qPCR system (Agilent ...
-
bioRxiv - Bioengineering 2023Quote: ... containing 10 μL 2X Luna® Universal Probe qPCR Master Mix (M3004, New England Biolabs, USA) (final concentration 1X) ...
-
bioRxiv - Microbiology 2023Quote: ... probe-based qPCR was carried out using Luna Universal Probe qPCR Master Mix (New England Biolabs) and the QuantStudio3 Real-Time PCR System (Thermo Fisher ...
-
bioRxiv - Neuroscience 2023Quote: ... RT-qPCR was performed by using Luna Universal One-Step RT-qPCR reagents (New England Biolabs), following the manufacturer’s instructions.
-
bioRxiv - Plant Biology 2024Quote: ... RT-qPCR was performed in technical triplicates using the Luna® Universal qPCR Master Mix (NEB) in a CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad ...
-
bioRxiv - Microbiology 2023Quote: ... RT-qPCR was then performed using Luna Universal One-step RT-qPCR kit (New England Biolabs) following the provided protocol on a BioRad CFX Opus 96 quantitative Real-Time PCR machine ...
-
bioRxiv - Molecular Biology 2021Quote: ... A preadenylated universal linker (5’-rAppCTGTAGGCACCATCAAT-NH2-3’) was prepared in house or purchased from NEB (S1315S) and ligated to the 3’ ends of the dephosphorylated footprints using T4 RNA Ligase 2 ...
-
bioRxiv - Molecular Biology 2020Quote: ... real time PCR was performed in triplicate using Luna® Universal qPCR Master Mix (New England Biolabs) with variable amounts of cDNA and 0.5 μM of target-specific primers in a CFX connect instrument (Bio-Rad) ...
-
bioRxiv - Microbiology 2019Quote: ... we performed q-RT PCR using the Luna Universal One-Step RT-qPCR Kit from NEB (E3005), using the Applied BiosystemsΤΜ 7500 Real-Time PCR system ...
-
bioRxiv - Cell Biology 2019Quote: ... 0.25μl of each primer (from 25μM stock) and 4.5μl of Luna Universal Master Mix (New England Biolabs). The q-RT-PCR reactions were run on the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems) ...
-
bioRxiv - Biochemistry 2020Quote: ... A master mix was prepared using the Luna® Universal qPCR Master Mix (New England BioLabs, M3003). DNA was amplified and quantified using a Roche LightCycler 480 (Roche Diagnostics Ltd ...
-
bioRxiv - Cell Biology 2020Quote: ... Multiplex quantitative PCR was then performed using the Luna Universal Probe qPCR Master Mix (ref M3004, NEB), a Roche LightCycler or Rotor Gene Q real-time PCR (Qiagen ...
-
bioRxiv - Neuroscience 2021Quote: ... qPCR reactions were performed in 20μl total volume with Luna Universal qPCR Master Mix (New England Biolabs). Target genes and reference controls were analysed in duplicate reactions for all samples ...
-
bioRxiv - Plant Biology 2021Quote: ... Nextera-compatible universal adapters [46] were ligated to the product (T4 DNA ligase; New England Biolabs, USA), and i5 and i7 PCR amplification was done (Q5 polymerase ...
-
bioRxiv - Plant Biology 2021Quote: ... The cDNA was used as a template for quantitative PCR using Luna Universal qPCR Master Mix (NEB) and a Viia7 real-time PCR instrument (Life Technologies/ABI) ...
-
bioRxiv - Microbiology 2022Quote: ... The SYBR Green assay was performed with the Luna Universal qPCR Master Mix (NEB, Ipswich, MA, USA) according to the manufacturer’s instructions with primers specific for HSV-1 gB (UL27 gene ...
-
bioRxiv - Cell Biology 2022Quote: ... Each reaction contained 4 µL of 2X Luna Universal qPCR Master Mix (New England Biolabs, cat#M3003E), 0.4 µL of each 20X primer assay ...
-
bioRxiv - Cell Biology 2022Quote: Each reaction contained 4 µL of 2X Luna Universal qPCR Master Mix (New England Biolabs, cat#M3003E), 0.4 µL of each 20X primer assay ...
-
bioRxiv - Microbiology 2020Quote: ... RT-qPCR was performed using Luna® Universal One-Step RT-qPCR kit (New England Biolabs; #E3005L). Briefly ...
-
bioRxiv - Plant Biology 2021Quote: ... qPCR was performed using Kapa SYBR FAST qPCR Master Mix or Luna Universal qPCR Master Mix (NEB) on a QuantStudio 3 system ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... and IFIH1 were quantified using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs #E3005L) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... Equimolar amounts of universal scaffold primer and target region primers were used with Q5 DNA polymerase (NEB). Cycling parameters included a touchdown PCR protocol ...
-
bioRxiv - Synthetic Biology 2019Quote: ... qPCR experiments were performed with the Luna Universal qPCR Master Mix (New England Biolabs, Ipswich, MA, USA). Primer pairs efficiencies for the housekeeping gene rpoB and target gene msfGFP can be seen in Supplementary Information C ...
-
bioRxiv - Cell Biology 2020Quote: ... or the Luna Universal Probe qPCR Master Mix (2x) Gene Expression (New England BioLabs # M3004L, Ipswich, MA), both mixes with the Rox reference dye option ...
-
bioRxiv - Plant Biology 2020Quote: ... followed by qRT-PCR master mix preparation using Luna® Universal qPCR Master Mix (NEB Biolabs, USA) which was finally performed in 7500 Fast Real-Time PCR System (Invitrogen ...
-
bioRxiv - Plant Biology 2020Quote: ... followed by qRT-PCR master mix preparation using Luna® Universal qPCR Master Mix (NEB Biolabs, USA) which was finally performed in 7500 Fast Real-Time PCR System (Invitrogen ...
-
bioRxiv - Genetics 2020Quote: ... and amplified by PCR with barcoded universal primers NEBNext Multiplex Oligos for Illumina (#E7335, New England Biolabs), using Kapa HiFi Polymerase (KAPA Biosystems) ...
-
bioRxiv - Microbiology 2021Quote: ... 20 μL qPCR reactions were prepared using the LUNA® Universal qPCR Master Mix (New England Biolabs), together with 2.5 μL of ChIP DNA (1:10 ...
-
bioRxiv - Cancer Biology 2022Quote: ... using QuantStudio™ Design & Analysis Software (v1.5.1) following Luna® Universal qPCR Master Mix (New England Biolabs) protocol and amplification parameters ...
-
bioRxiv - Cell Biology 2022Quote: ... 0.2 μM reverse primer and 5 μL Luna® Universal Probe qPCR Master Mix (New England Biolabs)] amplified using the Prism7900HT sequence detection system (Applied Biosystems ...
-
bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using the Luna universal one-step RT-qPCR kit (#E3005, New England Biolabs) according to the provided protocol ...
-
bioRxiv - Genetics 2022Quote: ... and SYBR green master mix using the Luna universal qPCR master mix (New England Biolabs, Ipswich, USA) for a 25 μl reaction ...
-
bioRxiv - Microbiology 2023Quote: ... Transcript levels of individual genes were determined by the Luna Universal One-Step RT-PCR kit (NEB) using approximately 100-200 ng of total RNA per sample as input ...
-
bioRxiv - Microbiology 2022Quote: ... including 2μL of gDNA and 10μL Luna® Universal Probe qPCR Master Mix (New England Biolabs, Australia). Final concentrations of the forward (S0947 ...
-
bioRxiv - Molecular Biology 2023Quote: ... All qRT-PCR reactions were performed with the Luna Universal One-Step RT-qPCR kit (E3005 NEB) according to manufacturer protocol with a few modifications ...
-
bioRxiv - Genomics 2023Quote: ... and Illumina sequencing adaptors were added using the Illumina TruSeq Universal Adapter and TruSeq_Index primer (NEB E7335S). Libraries were sequenced using the Illumina NovaSeq PE150 targeting 400 million reads per sample.
-
bioRxiv - Immunology 2023Quote: ... expression levels were determined using the Luna Universal One-Step RTqPCR kit (New England Biolabs catalog #E3005X) per manufacturer’s instructions ...
-
bioRxiv - Physiology 2023Quote: ... using either Luna Universal qPCR master mix for Sybr Green primers (New England Biolabs, Cat. No. M3003X) or Taqman Fast Advanced Master mix (ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: RT-qPCR experiments were performed with the Luna® Universal One-Step RT-qPCR Kit (NEB, #E3005E) with a final reaction volume of 10 μL per well ...
-
bioRxiv - Microbiology 2023Quote: ... and RpoD control were assessed using the Luna Universal One-Step qRT-PCR kit (New England Biolabs) according to the manufacturer’s instructions ...