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Citations for New England Biolabs :
1 - 50 of 615 citations for Universal TT epitope P2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... P2 adapter was ligated using concentrated T4 DNA ligase (NEB) and 50 ng of the ligated product was engaged in a 12 cycles PCR ...
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bioRxiv - Molecular Biology 2022Quote: ... were annealed to an oligonucleotide containing the antisense of the 3’ restriction site (5’-GGT TGA TTA TCG ATA AGC TT-3’) and extended using Klenow Polymerase devoid of exonuclease activity (NEB). Resulted fragments were inserted into the pAAV-CAG-hFXN plasmid between the stop codon of the hFXN and poly A signal ...
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bioRxiv - Molecular Biology 2023Quote: ... Illumina Universal Adaptor (NEB) was ligated to blunted end after adenylation ...
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bioRxiv - Molecular Biology 2022Quote: ... The 3xFLAG epitope was introduced by Gibson-style cloning (NEBuilder HiFi, NEB) at the C-terminus of the construct ...
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bioRxiv - Genetics 2023Quote: ... COL3A1-5’ UTR fragment was created from the reference allele (TT) PCR product using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs, Massachusetts, USA). The sequence of primers carrying out the alternative allele ...
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bioRxiv - Microbiology 2020Quote: ... The resulting P1/P2 and P3/P4 amplicons were digested with AscI and FseI (New England Biolabs) and ligated in separate bimolecular reactions to an ermAM erythromycin resistance cassette with compatible 5’ AscI and 3’ FseI overhangs ...
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bioRxiv - Biophysics 2023Quote: ... 240 pmol of each primer (P1 and P2) and Phusion 2X mastermix with HF buffer (NEB, M0531S). The PCR mix was split into 100 μl aliquots and subjected to 35 cycles of 10 s 98 °C denaturation ...
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bioRxiv - Developmental Biology 2022Quote: ... Luna Universal qPCR Master Mix (NEB) was used to amplify the DNA fragment ...
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bioRxiv - Microbiology 2020Quote: ... Universal miRNA Cloning Linker – S1315S (Biolabs) and in-house designed indexed primers ...
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bioRxiv - Developmental Biology 2022Quote: Luna Universal qPCR Master Mix (NEB) was used to amplify 1.5 µl of each cDNA sample in a 20 µl total reaction using the manufacturer’s standard protocol on an Applied Biosystems StepOne Real-Time PCR System (Thermo) ...
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bioRxiv - Genomics 2019Quote: ... Methylated universal adaptor (NEB Cat. E7535) was ligated using the Blunt/TA ligase from the kit ...
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bioRxiv - Molecular Biology 2020Quote: ... Luna Universal qPCR Master Mix (NEB), and the primers listed in supplementary table S1 ...
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bioRxiv - Immunology 2020Quote: ... with Luna Universal qPCR Mastermix (NEB). For analysis of Aicda mRNA decay ...
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bioRxiv - Developmental Biology 2024Quote: ... Luna Universal qPCR Master Mix (NEB) was used to amplify 2.0 microliters of each cDNA sample in a 20 microliter total reaction using the standard protocol on an Applied Biosystems StepOne Real-Time PCR system (Thermo) ...
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bioRxiv - Microbiology 2024Quote: ... The myc epitope was added before the stop codon of blaSHV-5 by NEB Q5 Site-Directed Mutagenesis according to the manufacturer using primers JCP505/506 with pTOX5 blaSHV-5 template ...
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bioRxiv - Developmental Biology 2020Quote: ... using Luna Universal qPCR Master Mix (NEB), and the data were analyzed by LightCycler software.
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bioRxiv - Cell Biology 2022Quote: ... or LUNA Universal qPCR Master Mix (NEB) on a 7500 real-time PCR machine (Applied Biosystems) ...
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bioRxiv - Genomics 2019Quote: ... A Universal miRNA cloning linker (NEB # S1315S) was ligated to the 3’-ends of the RNA using T4 RNA Ligase 2 (NEB #M0242L ...
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bioRxiv - Cell Biology 2022Quote: ... and Luna Universal qPCR Master Mix (NEB) in the CFX Real-Time PCR system (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Luna® Universal qPCR Master Mix (NEB) was used to quantify mRNA levels for each cell line ...
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bioRxiv - Physiology 2021Quote: ... LUNA Universal Probe qPCR Mastermix (NEB, #M3004E) and 1,5 μM of primers in a total volume of 5μl were used ...
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bioRxiv - Molecular Biology 2022Quote: ... using Luna Universal qPCR Master Mix (NEB). Primers for rpl-12(PTC ...
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bioRxiv - Plant Biology 2023Quote: ... Luna® Universal qPCR Master Mix (NEB) was used for qPCR on an ABI StepOne Plus real-time system (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... Luna Universal qPCR Master mix (NEB, UK) was used for qPCR according to manufacturer’s recommendations (Initial denaturation 95°C ...
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bioRxiv - Genetics 2021Quote: ... primers P1 and P2 (Table S1) were used with the Q5 Hot Start High-Fidelity 2X Master Mix (NEB). To ensure coverage for each sample ...
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bioRxiv - Microbiology 2021Quote: ... A K13 propeller domain-specific guide gRNA was introduced into this vector at the BbsI restriction sites using the oligo pair p1+p2 (Table S9) using T4 DNA ligase (New England BioLabs). Oligos were phosphorylated and annealed prior to cloning ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We then ligated Illumina P2 adaptors to the size-selected libraries and amplified P1/P2-adapted fragments with 12 cycles of PCR using Phusion-HF polymerase (New England Biolabs). RAD libraries were then sequenced in a single lane on an Illumina HiSeq 2500 to generate paired-end 250-bp sequence reads ...
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bioRxiv - Physiology 2021Quote: ... A K13 propeller domain-specific guide gRNA was introduced into this vector at the BbsI restriction sites using the oligo pair p1+p2 (Supplementary file 5) using T4 DNA ligase (New England BioLabs). Oligos were phosphorylated and annealed prior to cloning ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and P2 adaptor ligation steps were performed using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs). After size selection using AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... P1 and P2 barcoded adapters corresponding to the restriction site of the enzymes were ligated immediately afterwards with T4 ligase (NEB) at 16°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... Universal PCR primers and Index (X) Primer (NEB). PCR products were then purified (AMPure XP system ...
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bioRxiv - Molecular Biology 2020Quote: ... Luna Universal qPCR Master Mix (New England Biolabs), following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... using the Luna Universal qPCR master mix (NEB). TATA-box binding protein (Tbp ...
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bioRxiv - Evolutionary Biology 2020Quote: ... using a Luna Universal qPCR Master Mix (NEB) at the fast setting following the manufacturer’s instructions to determine dCT levels in triplicate ...
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bioRxiv - Immunology 2022Quote: ... Luna Universal qPCR Master Mix (New England Biolabs) was used for quantification of fungal ITS2 sequences and TaqMan Gene Expression Assays (Life Technologies ...
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bioRxiv - Cancer Biology 2022Quote: The Luna Universal qPCR Master Mix (NEB, M3003) was used for qPCR employing 2 μL of cDNA sample and 18 μL mix of master mix (water ...
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bioRxiv - Molecular Biology 2022Quote: ... Luna Universal qPCR master mix (New England Biolabs) was used with 600 pM primer mix and 5 ng DNA per reaction ...
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bioRxiv - Cell Biology 2022Quote: ... the Luna Universal qPCR Master Mix protocol (NEB) was followed.
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bioRxiv - Cell Biology 2021Quote: ... using Luna Universal qPCR Master Mix (NEB, M3003L). Sequences of all primers used are listed in Suppl ...
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bioRxiv - Microbiology 2021Quote: ... Universal miRNA Cloning Linker (NEB, cat. No. S1315S), Superscript III (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... qPCR was performed using Luna Universal Mastermix (NEB), denaturation/annealing/elongation was performed for 50 cycles ...
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bioRxiv - Molecular Biology 2022Quote: ... using Luna Universal qPCR Master Mix (NEB, M3003L). For splicing verification ...
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bioRxiv - Cancer Biology 2022Quote: ... Luna Universal qPCR MasterMix (NEB, cat. no. M3003) was used according to manufacturer’s specifications ...
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bioRxiv - Cell Biology 2024Quote: ... using Luna Universal qPCR Master Mix (NEB, M3003L). Sequences of all primers used can be found in Suppl ...
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bioRxiv - Molecular Biology 2019Quote: For the oligo-dT affinity chromatography 0.5 ml of C1 or P2 fractions were incubated with an equal volume of a 50% slurry of oligo(dT) beads (NEB #S1408S) in Phy buffer ...
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bioRxiv - Genomics 2019Quote: RNA from P2 and P9 hypothalamus and cortex was prepared for RNA-seq library using the NEBNext rRNA Depletion kit (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760S) ...
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bioRxiv - Biochemistry 2022Quote: The P562del and Exo+THR mutants were generated on the pET23-P2-D12A-THR (Table S1) background through inverse Polymerase Chain Reactions (iPCR) using Q5® High-Fidelity DNA Polymerase (NEB) following the manufacturer’s instructions in 25 μl reactions with primers p2_thumb_loop_R/DEL1 (Table S2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... reaction was performed to remove sfGFP and replace it with the cassette components (P1-FLAG, P2-Substrate, P3-HA, P4-ERS). This plasmid was transformed into competent Escherichia coli (E. coli) (NEB#C2984H), purified (QIAGEN #27106 ...
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bioRxiv - Microbiology 2019Quote: ... and also quantified using NEB Luna Universal qPCR Master Mix and Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA) for qPCR and RT-qPCR test ...
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bioRxiv - Molecular Biology 2020Quote: ... ligated to the linker (NEB Universal miRNA Cloning Linker) using T4 RNA ligase (NEB) ...