Labshake search
Citations for New England Biolabs :
401 - 450 of 615 citations for Universal TT epitope P2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... 1% of the cDNA was used for each qPCR reaction performed with the Luna Universal qPCR Mastermix (NEB M3003) and the PCR primers listed (Supplementary Table1) ...
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bioRxiv - Genomics 2021Quote: The qPCR reactions were performed with enzymatic selection or control samples using Luna Universal qPCR Master Mix (NEB #M3003S) on a Bio-Rad CFX96 real-time PCR detection system ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µl was added to a 9 µl Luna® Universal One-Step RT-qPCR reaction (NEB, Cat# E3005L). RT-qPCR was carried out to manufacturer specifications in a CFX96 Touch Real Time PCR Machine (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real time PCR (qRT-PCR) was performed with the Luna Universal qPCR Master Mix (New England Biolabs #M3003) on a CFX Connect instrument (BioRad #1855200) ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative real time PCR (qRT-PCR) was performed with the Luna Universal qPCR Master Mix (New England Biolabs #M3003) on a CFX Connect instrument (BioRad #1855200) ...
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bioRxiv - Microbiology 2021Quote: ... using the IP4 set of primers and probe as described on the WHO website (https://www.who.int/docs/default-34source/coronaviruse/real-time-rt-pcr-assays-for-the-detection-of-sars-cov-2-institut-35pasteur-paris.pdf?sfvrsn=3662fcb6_2) and the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs, France).
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative RT-PCR (qRT-PCR) was performed in duplicate with the Luna® Universal qPCR Master Mix (NEB, M3003) using QuantStudio 5 Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was subjected to OneStep qRT-PCR analysis using Luna Universal One-Step RT-qPCR Kit (New England Biolabs) or Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was then performed in 384 well format with Luna Universal Probe One-Step RT-qPCR Kit (NEB), assaying Fluc (primers oHR711/712 ...
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bioRxiv - Microbiology 2020Quote: ... SCV2 RNA was quantified using a NEB Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs, E3006) and 2019-nCoV CDC N1 primers and probes (IDT ...
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bioRxiv - Cell Biology 2022Quote: ... qPCR was performed in triplicate with Luna® Universal qPCR SYBR Green Master Mix (New England BioLabs, catalog #M3003L), where 25 ng cDNA was loaded per well ...
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bioRxiv - Genetics 2019Quote: ... 10 pmoles of forward and universal reverse primers were annealed and extended with Q5 high-fidelity DNA polymerase (NEB) according to the recommended conditions and with the following program ...
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bioRxiv - Plant Biology 2019Quote: ... qRT-PCR was performed with a oligonucleotide concentration of 300 nM using the Luna Universal qPCR Master Mix (NEB). The results were analyzed using the Applied Biosystems 7500 Fast Real-Time PCR Software v2.3 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... RT-qPCR was conducted in technical triplicate using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) according to the manufacturer’s instructions in 10 µl reaction volumes and reactions were run on a CFX Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... THUNDERBIRD Probe qPCR Master Mix (Toyobo) for MERS-CoV and Luna Universal Probe One-Step RT-qPCR Kit (NEB) for SARS-CoV-2.
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bioRxiv - Evolutionary Biology 2020Quote: ... The libraries were amplified and both the indexed and universal primer (NEBNext Multiplex Oligos for Illumina, New England Biolabs) were added by PCR using HGS Diamond Taq DNA polymerase (Eurogentec) ...
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bioRxiv - Microbiology 2021Quote: ... 20 μl qPCR reactions were prepared in duplicate as follows: 1x Luna Universal Probe qPCR Master Mix (NEB M3004L), 300 nM of forward and reverse primer ...
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bioRxiv - Microbiology 2020Quote: ... RdRP_SARSr-F2 (GTGARATGGTCATGTGTGGCGG) and dRP_SARSr-R1(CARATGTTAAASACACTATTAGCATA) using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) and a CFX96 Real-Time System ...
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bioRxiv - Microbiology 2021Quote: ... Or reverse transcription and qRT-PCR were conducted in one step using 0.1 μg RNA as template with the Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs) following manufacturer’s protocols ...
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bioRxiv - Genetics 2020Quote: ... The overall abundance of P0 gRNA genes was quantified with Luna® Universal qPCR Master Mix (NEB, Cat: M3003S) using primer pair F/R’ (Fig ...
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bioRxiv - Microbiology 2022Quote: ... and real-time PCR was performed using the Luna Universal qPCR Master Mix (New England Biolabs, Ipswich, MA, USA) on an ABI 7500 Fast RT-PCR system (Thermo Fisher ...
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bioRxiv - Developmental Biology 2022Quote: ... The synthesized cDNAs were then used for quantitative PCR by using Luna Universal qPCR Master Mix (New England Biolabs). The following PCR Primers were used in this study ...
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bioRxiv - Biochemistry 2022Quote: ... The lysed cells were directly subjected to qRT-PCR using Luna Universal One-Step RT-qPCR Kit (NEB, #E3005L). The primer sets used in qRT-PCR to detect CI-MPR and CD-MPR mRNA are 5’-CATTCAGTGGGTGACTCTGTT-3’/5’-TGCTCTGGACTCTGTGATTTG-3’ and 5’-GCTCTAGTGAAGAGGCTGAAAC-3’/ 5’-GCACACCCTGAAGATGTAGATG-3’ ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using Luna® Universal Probe One-Step RT-qPCR kit (E3006L; New England Biolabs (NEB), Ipswich ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using Luna® Universal Probe One-Step RT-qPCR kit (E3006L; New England Biolabs (NEB), Ipswich ...
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bioRxiv - Molecular Biology 2023Quote: ... ddH2O and 2μL of diluted cDNA were used in 10μL qPCR reactions containing the Luna® Universal qPCR Master Mix (NEB) and 0.3μM of gene-specific forward and reverse primer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Quantification of viral genomes was performed using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with a primer/probeset binding in the orf1ab region (forward ...
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bioRxiv - Microbiology 2023Quote: ... while the ter region was amplified using the primer pair 3778_ter 1 qPCR fwd with 3778_ter 1 qPCR rev and the Luna Universal qPCR Master Mix (New England Biolabs). Quantification of the ori/ter ratio was performed using the the 2-ΔCT method as described [62].
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bioRxiv - Immunology 2022Quote: ... quantitative reverse transcription PCR (qRT-PCR) was conducted using Luna universal probe one-step RT-PCR kit (NEB, #E3006) on CFX96 Touch Real-Time Detection System on 96-well plates (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... P13-P19) (73) were carried out using Luna Universal One-Step RT-qPCR Kit (New England Biolabs; Frankfurt (Main), Germany ...
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bioRxiv - Immunology 2023Quote: ... and the RT-qPCR master mix prepared using a Luna Universal One-Step RT-qPCR Kit (New England Biolabs), as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... qPCR was performed using 67.5ng of gDNA for each condition in 10µl reactions using Luna Universal qPCR Master Mix (NEB, M3003E).
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bioRxiv - Genomics 2023Quote: ... We performed qPCR on a StepOneTM Real-Time PCR System with the Luna® Universal qPCR Master Mix (NEB). Gene specific primers are outlined in Extended Data Table S4.
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bioRxiv - Plant Biology 2022Quote: ... were analyzed by Quantitative real time PCR (qRT PCR) using Luna® Universal qPCR Master Mix (New England BioLabs) in a QuantStudio® 5 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA expression was determined using SYBR green Luna Universal One-step RT-PCR kit (New England Biolabs, Cat #E3005L) with a BioRad CFX96 thermocycler ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using 10-fold-diluted cDNA and the Luna Universal qPCR Master Mix (New England Biolabs) in the CFX Real-Time PCR system (Bio-Rad) ...
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bioRxiv - Genomics 2023Quote: ... Ten-fold diluted cDNA was used in qRT-PCR using Luna Universal qPCR Master Mix (New England Biolabs #M3003E) in a final 10 µL reaction in QuantStudio 3 Real-Time PCR machine (Thermo Fisher Scientific) ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... The quantitative PCR reaction mix contained 5 µl of the Luna Universal qPCR Master Mix (2X, New England BioLabs), 1 µl of the forward primer (10 µM) ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription of RNA and quantitative PCR was performed using the Luna Universal One-step RT-qPCR Kit (NEB) using 1 µg of RNA per reaction ...
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bioRxiv - Developmental Biology 2024Quote: ... and both reverse transcription and PCR were performed using the Luna One-Step Universal RT-qPCR kit (NEB, E3005S) and carried out on the Mic qPCR Machine (BioMolecular Systems ...
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bioRxiv - Genomics 2024Quote: ... libraries were quantified in triplicate using NEB Luna® Universal Probe qPCR Master Mix (New England Biolabs, Ipswitch, MA), Illumina PhiX standard (San Diego ...
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bioRxiv - Cell Biology 2024Quote: ... qPCR was performed on the synthesised cDNA using the Luna Universal qPCR Master Mix kit (New England Biolabs, USA). 4 μL of cDNA was added to 1 μL of pre-purchased primers (QuantiTect primers (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... qRT-PCR was performed on cDNA from samples for genes of interest using the Luna Universal qPCR Master Mix (#M3003, New England Biolabs) according to manufacturer’s specifications ...
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bioRxiv - Genetics 2021Quote: ... One-tenth of the RT reaction was used as a template for real-time PCR using Luna Universal qPCR Master Mix (New England Biolabs) on a QuantStudi 6 system ...
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bioRxiv - Microbiology 2021Quote: ... Official CDC SARS-CoV-2 N1 gene primers and TaqMan probe set were used [58] with the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs):
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bioRxiv - Molecular Biology 2021Quote: ... The mRNA abundance levels were determined using 200 ng total RNA for each sample in technical replicates using Luna Universal One-Step RT-qPCR kit (New England BioLabs) and SYBR Green as detection agent and gene-specific primers (Table S2) ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μl of Universal nuclease (Pierce; 125U/L of culture) and 10 μl of DNaseI (NEB; 20U/L of culture) were added and the mixture allowed to incubate for 10 min at RT ...
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bioRxiv - Microbiology 2019Quote: ... in 20 µL reaction volume including: 10 µL Luna Universal qPCR Master Mix (New England Biolabs, Inc., Cat. No. NC1276266), primers listed in Table 2 ...
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bioRxiv - Microbiology 2019Quote: ... samples were diluted to 5ng/uL and used for qRT-PCR with Luna One-Step Universal qPCR kit (NEB E3005). Gene specific primers for target transcripts were used at a final concentration of 400uM with 15ng of RNA ...
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bioRxiv - Plant Biology 2019Quote: ... RT-PCR reaction volumes were set up to 12.5 μL containing Luna Universal qPCR Master Mix (New England Biolabs Inc.), 2 μl of a 1:10 dilution of cDNA reaction ...