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Citations for New England Biolabs :
401 - 450 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... DNA was digested into single nucleosides by using the Nucleoside Digestion Mix (New England Biolabs) enzyme cocktail at 37°C for 2 h ...
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bioRxiv - Genomics 2022Quote: ... Original vector backbone and intermediate backbone product were designed for digestion with Esp3I (BsmBI, NEB), inserts were designed for digestion with BsaI (NEB).
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bioRxiv - Biochemistry 2023Quote: ... was cloned into pMIG-GFP plasmid by restriction digestion method using Bgl2 and EcoR1 (NEB). Primers used for cloning (Forward primer ...
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bioRxiv - Synthetic Biology 2024Quote: Cloning was performed using restriction enzyme digestion and ligation with T4 ligase (New England Biolabs) or Gibson assembly (New England Biolabs) ...
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bioRxiv - Immunology 2023Quote: ... After digestion the linearized backbone was dephosphorylated by addition of 2 µl rSAP (NEB, #M0371S) and incubation at 37 °C for 1 hour followed by heat inactivation of the enzymes at 80 °C for 20 minutes ...
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Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA)bioRxiv - Biochemistry 2023Quote: ... The parent DNA template was removed via enzymatic digestion with restriction enzyme DpnI (NEB #R0176S) and the PCR amplified fragments were gel purified ...
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bioRxiv - Microbiology 2024Quote: ... the product of the digestion reaction was dephosphorylated by the Quick CIP (New England Biolabs). For ligation of the vector and the PCR product ...
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bioRxiv - Molecular Biology 2021Quote: ... Analytical amounts of twenty Argonaute proteins were synthesized from pET29a plasmids using PURExpress In Vitro Protein Synthesis kit (New England Biolabs, Inc., Ipswich, MA, USA). For large scale expression and purification of CbAgo ...
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bioRxiv - Microbiology 2020Quote: Fetuin was deglycosylated under denaturing conditions using Protein Deglycosylation Mix II kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... PhyR1 and PhyR2 proteins were overexpressed and purified using the IMPACT kit (New England Biolabs) following the manufacturer’s instructions and equal procedures for the four of them ...
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bioRxiv - Molecular Biology 2019Quote: ... in vitro translation was performed using the PURExpress In vitro Protein Synthesis kit (NEB 6800) and the non-stop ribosome complex was purified as previously described in ref.38 ...
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bioRxiv - Biophysics 2022Quote: ... The protein was labeled with SNAP-Surface Alexa Fluor 647 labeling kit (New England Biolabs) according to the protocol provided by the manufacturer ...
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bioRxiv - Biophysics 2022Quote: Plasmids expressing stdMCP-fusion proteins were generated using the NEBuilder HiFi DNA Assembly kit (NEB). To obtain plasmids carrying an stdGFP-tagged stdMCP gene ...
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bioRxiv - Microbiology 2021Quote: ... the plasmid digestion was supplemented with 1.5 units of recombinant shrimp alkaline phosphatase (rSAP; NEB #M0371S). Digested insert and vector were purified and ligated together at room temperature for 30 min using T4 DNA ligase (NEB ...
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bioRxiv - Genomics 2022Quote: ... The mtDNA digestion was followed by incubation with 0.12 units of Thermolabile Proteinase K treatment (NEB) for 15 min at 37°C and 10 min at 55°C for inactivation ...
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bioRxiv - Genomics 2019Quote: Genomic control DNAs were digested to nucleosides by treatment with the Nucleoside Digestion Mix (NEB, M0649S) for 1 h at 37 C ...
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bioRxiv - Microbiology 2021Quote: ... The pGHJ-TgApiAT6-1 and pGHJ-TgApiAT1 [27] plasmids were linearized by digestion with NotI (NEB) for 2 hr ...
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bioRxiv - Genetics 2019Quote: ... The PCR products were engineered for digestion using Kpn1 and Hind3 restriction enzymes (New England Biolabs) and inserted into the pGL4.23 vector (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... mutants were confirmed by Sanger sequencing (Australian Genome Research Facility, Brisbane) or digestion with αTaqI (NEB).
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bioRxiv - Microbiology 2021Quote: ... eluted in 60 μL and subjected to DNase I digestion (New England Biolabs Ltd, cat #: M0303S) to minimize the risk of amplifying HIV DNA in these low pVL samples ...
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bioRxiv - Plant Biology 2020Quote: The plant expression vector pER8 (GenBank: AF309825.2) was linearized by digestion with XhoI and SpeI (NEB). To develop the pER-GUS construct ...
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bioRxiv - Cell Biology 2022Quote: Transforming plasmids were linearized by digestion with either ScaI-HF® or AseI restriction enzymes (NEB), which cut within the ampicillin resistance gene ...
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bioRxiv - Neuroscience 2022Quote: ... the vector was linearized by double digestion using BamHl and EcoRl (New England Biolabs, Ipswich, MA) and purified by the GeneJET gel extraction kit (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmid pl21 (TriAltus) was truncated by digestion with restriction enzymes XbaI and XhoI (New England Biolabs). To complete the recombinant assembly reaction NEBuilder® HiFi DNA Assembly kit (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... enzyme digestion or Peptide-N-Glycosidase F (PNGase F) (New England Biolabs, Ipswich, MA, catalog #: P0704L) enzyme digestion was performed according to manufacturer’s instruction and the published procedure [49].
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bioRxiv - Neuroscience 2022Quote: ... Gels were then placed in digestion solution containing proteinase K (8U/mL, New England BioLabs, P8107S) and digested for 4 hours at room temperature.
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bioRxiv - Pathology 2020Quote: ... DNAs were subsequently cloned into destination vectors after digestion by corresponding restriction enzymes (New England Biolabs) and ligation with T4 DNA ligase (Promega) ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by digestion of the wild-type strand with the enzyme BsurI (New England Biolabs, R0581S)[27] ...
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bioRxiv - Molecular Biology 2021Quote: ... the reaction product was combined with 20 μl digestion mix containing 5 μl ExoI (NEB, M0293S), 5 μl HinFI (NEB ...
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bioRxiv - Biophysics 2020Quote: ... The looping buffer was replaced with 200 μl of digestion buffer (6.66 μl of RecBCD (NEB), 20 μl NEB 10X Buffer 4 ...
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bioRxiv - Microbiology 2022Quote: ... and the internal 504 bp of pilT was removed by digestion with AccI (New England Biolabs). The plasmid was religated with T4 DNA ligase (New England Biolabs ...
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bioRxiv - Developmental Biology 2022Quote: ... The plasmid was linearized via EcoRV restriction enzyme digestion using EcoRV-HF (New England Biolabs #R3195S) before Gibson assembly.
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bioRxiv - Plant Biology 2022Quote: ... Plasmids were also confirmed through digestion with the restriction enzyme EcoRI (New England Biolabs; Ref.: R3101S) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... 10 ul of diluted DNA were used for McrBC digestion (NEB, 4 h at 37 °C) or mock digestion (the same volume of H2O instead of McrBC enzyme was added with all other components the same in the reaction ...
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bioRxiv - Microbiology 2022Quote: ... A DNA digestion was then completed using the restriction enzymes BamHI and XbaI (New England Biolabs) on both the PA3626 product and pUCP18 plasmid following the New England Biolabs protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... the pUC18 plasmid was subjected to digestion with the EcoRI-HF restriction enzyme (New England Biolabs), followed by a standard ligation reaction ...
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bioRxiv - Developmental Biology 2023Quote: ... The amplicon was subcloned into pN2-CMV:RFP plasmid using XhoI/EcoRI restriction enzyme digestion-ligation (NEB) to generate pN2-CMV:AmRps14-RFP plasmids and sequence verified ...
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bioRxiv - Immunology 2023Quote: ... the pMXs-SOX2-IP construct was linearized by digestion with PacI and EcoRI restriction enzymes (NEB) to cut the SOX2 gene (pMXs-IP) ...
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bioRxiv - Molecular Biology 2023Quote: ... BAC integrity was assessed by digestion with the restriction enzymes RsrII and SbfI (New England Biolabs) and by full plasmid sequencing (Plasmidsaurus).
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bioRxiv - Neuroscience 2023Quote: ... Gels were then placed in digestion solution containing proteinase K (8U/mL, New England BioLabs, P8107S) and digested for 4 hours at room temperature.
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bioRxiv - Cancer Biology 2023Quote: ... we prepared a digestion mix containing 100 nL of NlaIII restriction enzyme (NEB, cat. no. R0125L) and 50 nL of CutSmart buffer (NEB ...
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bioRxiv - Microbiology 2023Quote: ... 200 ng of poly(A)+ RNA were digested with a nucleoside digestion mix (New England Biolabs) at 37°C for one hour ...
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bioRxiv - Biophysics 2023Quote: ... The 12mer DNA sequence was excised from the plasmid using Eco-RV digestion (New England BioLabs), and the excess plasmid was removed via PEG purification ...
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bioRxiv - Genomics 2024Quote: ... 4 μg of the barcoded plasmid library was linearized by digestion with NruI-HF (NEB #R3192) at 37°C for 16 hours ...
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bioRxiv - Genomics 2024Quote: ... Enzymatic digestion was performed using enzymes Taq1-v2 and Pst1-HF (New Englands Biolabs, 20assachusetts, USA), in agreement with the simulation results of Herry et al (2023) ...
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bioRxiv - Microbiology 2024Quote: ... Chimeras were cloned in plasmid pSHU1234 using restriction digestion with NdeI and PstI (New England Biolabs). Plasmids carrying chimeric constructs were used as templates to generate the double-stranded donor DNA used to replace the motA and motB gene on the RP437 chromosome with pomA and potD variants ...
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bioRxiv - Microbiology 2024Quote: ... the isolated DNA was processed to single nucleosides by applying Nucleoside Digestion Mix (NEB, MA, USA).
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bioRxiv - Immunology 2023Quote: Plasmids with CAR constructs were linearized by digestion with the restriction enzyme SalI (New England Biolabs). mRNA was produced using the mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit ( AMB13455 ...
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bioRxiv - Bioengineering 2021Quote: 25 µl reactions were set up following the PURExpress protocol (In Vitro Protein Synthesis Kit, NEB) in black low-volume 384-well polystyrene plates (Corning) ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein and non-incorporated nucleotides were removed using the Monarch RNA Clean up kit (NEB, T2040), before quantification by nanodrop.