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Citations for New England Biolabs :
301 - 350 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... Donors were replaced via double digestion by SpeI and AvrII (New England Biolabs). Plasmids were amplified using Stbl3 competent cells prepared with The Mix & Go! E ...
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bioRxiv - Microbiology 2020Quote: ... BAC quality was assessed by digestion with RsrII and SbfI (New England Biolabs). Latently infected iSLK cell lines with modified virus were generated by transfection of HEK293T-ORF68 cells with 5 μg BAC DNA using PolyJet reagent (SignaGen) ...
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bioRxiv - Cell Biology 2019Quote: ... Chromatin was then solubilized by DNase I digestion (1 unit, New England Biolabs) in CSK buffer with Complete Protease Inhibitor for 15 min at 37 °C ...
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bioRxiv - Genetics 2020Quote: ... by digestion with SalI and NheI and ligation with T4 DNA ligase (NEB).
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bioRxiv - Genetics 2020Quote: ... Sheared DNA was further fragmented by digestion with MfeI-HF (New England Biolabs), a restriction enzyme that cuts frequently and between the engineered LCRs ...
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bioRxiv - Genomics 2021Quote: ssDNA-specific Digestion: 20µL cfDNA was combined with 1µL 0.1x P1 (NEB, M0660S), 3µL NEBuffer r1.1 ...
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bioRxiv - Genomics 2021Quote: ssDNA-specific Digestion: 20µL cfDNA was combined with 3µL Exonuclease 1 (NEB, M0293S), 3µL 10x Exo 1 Buffer ...
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bioRxiv - Biochemistry 2021Quote: ... and then digested with 2 μL Nucleoside Digestion Mix (#M0649, New England Biolabs) in a 50 μL reaction incubated at 37 °C overnight ...
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bioRxiv - Genomics 2021Quote: ... Samples were digested into nucleosides using Nucleoside digestion mix (M0649S, New England Biolabs) following manufacturers protocol ...
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bioRxiv - Microbiology 2021Quote: ... was subjected to restriction digestion by specific restriction enzymes such as NcoI (NEB) and HindIII (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... DNAse I digestion was carried out using RNAse free DNase I (M0303S- NEB), and reverse transcription on 1 μg of DNase treated total RNA was performed using RevertAid Reverse Transcriptase ...
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bioRxiv - Biochemistry 2022Quote: ... and nucleoside digestion mix were purchased from New England Biolab (NEB, Ipswich, MA). DNase was purchased from Roche (Basel ...
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bioRxiv - Biochemistry 2022Quote: ... was linearized by digestion with restriction enzymes BamHI and HindIII (New England Biolabs).
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bioRxiv - Neuroscience 2022Quote: ... into pUASTattB (Bischof et al., 2013) via BamHI digestion and T4 ligation (NEB). We linearized pQUASTattB or pUASTattB with EcoRI-XhoI to enable the insertion of transgenes via Gibson assembly (HiFi DNA Assembly ...
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bioRxiv - Synthetic Biology 2022Quote: ... or digestion and ligation using T4-DNA ligase (NEB, Frankfurt am Main, Germany). Plasmid and primer sequences are listed in Tables S2 and S3 ...
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bioRxiv - Microbiology 2023Quote: ... pACYC184 was linearized by BamHI and HindIII digestion (New England Biolabs, Ipswitch, MA). The locus for arnD ...
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bioRxiv - Genomics 2023Quote: ... CROP-seq-opti was linearized via digestion with BsmBI and alkaline phosphatase (NEB) with PCR clean up in between both digestions ...
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bioRxiv - Developmental Biology 2023Quote: ... Trypsin digestion was stopped by adding 5% BSA (ref. B9000S, New England Biolabs) and the cell suspension was passed through a 40 µm Flowmi cell strainer (ref ...
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bioRxiv - Genetics 2024Quote: ... 40 μl of MNAse digestion buffer (3.33 gelUnits/μl Micrococcal Nuclease (NEB M0247S), 1.25X Micrococcal Nuclease buffer ...
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bioRxiv - Genetics 2024Quote: ... 50 μl of MNAse digestion buffer (5 gelUnits/μl Micrococcal Nuclease (NEB M0247S), 2X Micrococcal Nuclease buffer ...
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bioRxiv - Microbiology 2023Quote: ... The plasmids were linearised by digestion with PacI (New England Biolabs, Ipswich, UK) and ethanol precipitated before transfection ...
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bioRxiv - Microbiology 2023Quote: ... and restriction digestion and DNA ligation was performed using enzymes supplied by NEB. Plasmids were transformed into NEB5a (NEB ...
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... 3C-libraries were prepared from thawed nuclei subjected to DpnII digestion (NEB, R0543M), re-ligated with T4 ligase (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The plasmid was linearized by digestion with SapI restriction enzyme (New England Biolabs) for 1 hour in 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... following linearization of the vector through restriction enzyme digestion using AgeI-HF (NEB) and SalI-HF (NEB) ...
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bioRxiv - Plant Biology 2024Quote: ... The presence of mutation was identified by T7 Endonuclease digestion (NEB M0302, www.neb.com). Homozygous lines were obtained for further study ...
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bioRxiv - Neuroscience 2024Quote: ... the vector was linearized by double digestion using restriction enzymes (New England Biolabs) and purified by the GeneJET gel extraction kit (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: ... the vector was linearized by double digestion using restriction enzymes (New England Biolabs). DNA fragments were generated by PCR amplification and then fused with the backbones using NEBuilder HiFi DNA assembly kit (New England Biolabs) ...
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bioRxiv - Immunology 2023Quote: ... and excess primers were removed with an Exonuclease I digestion (New England Biolabs). Whole transcriptome amplification (WTA ...
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bioRxiv - Synthetic Biology 2023Quote: The cell-free protein synthesis (CFPS) reactions were carried out using the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs Inc) in 384-well plates (Thermofisher NUNC ...
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bioRxiv - Microbiology 2023Quote: RdnE proteins were produced using the New England Biolabs PURExpress In Vitro Protein Synthesis Kit (New England BioLabs Inc., Ipswich MA). Template DNA contained the rdnE gene and required elements specified by the PURExpress kit ...
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bioRxiv - Cell Biology 2023Quote: ... a PCR fragment containing the complete Nv-osk coding sequence was synthesized for protein expression with PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs) using manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... DHFR control plasmid from PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) was used as backbone for subsequent ligations ...
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bioRxiv - Developmental Biology 2021Quote: ... Micrococcal nuclease digestion was completed with 200 U of micrococcal nuclease (New England Biolabs) at 21°C for 7.5 min ...
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bioRxiv - Cell Biology 2020Quote: ... and digestion mix was added (375 U DpnII [NEB] in 1x DpnII buffer [NEB]) and cells incubated for 16 h at 37 °C under rotation ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 ng of genomic DNA was subjected to MspI digestion (NEB, Beverly, MA, USA), with subsequent end repair/dA-tailing reaction using Klenow Fragment (3’-5’ exo- ...
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bioRxiv - Plant Biology 2021Quote: ... Overnight digestion at 37 °C was performed using 400U of Hind III enzyme (NEB). Digested DNA was ligated during 5 h incubation at 16 °C with 100 U of T4 DNA ligase (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... we performed test-digestion of the generated plasmids with restriction enzymes (New England Biolabs), and analyzed the fragment pattern via gel electrophoresis ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were digested using 2 Units/μL MNase in 20μL MNase Digestion Buffer (NEB) for 5 minutes at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The methylation status was assayed via enzymatic digestion using DNA-methylation-sensitive SmaI (NEB) and -insensitive MspI (NEB ...
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bioRxiv - Genomics 2020Quote: ... DNA samples were digested to nucleosides using the Nucleoside Digestion Mix (NEB, Ipswich, MA). Global nucleoside content analysis was performed by LC-MS or LC-MS/MS as noted (see conditions below) ...
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bioRxiv - Genetics 2022Quote: ... Digestions were performed according to the manufacturer’s instructions (New England BioLabs #P0702L and #P0704S) (dx.doi.org/10.17504/protocols.io.8epv59ew4g1b/v1 and (dx.doi.org/10.17504/protocols.io.cqfvtm) ...
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bioRxiv - Synthetic Biology 2022Quote: ... NLS was removed from restriction digestion using following restriction enzymes: EcoR1 (Cat # R0101; NEB) and Fse I (Cat # R588 ...
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bioRxiv - Immunology 2020Quote: ... The Fab fragments were generated by overnight digestion with Endoproteinase LysC (New England Biolabs) at 37 °C and purified by protein A column to remove uncut IgG and Fc fragments.
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bioRxiv - Cell Biology 2020Quote: ... The transfer vector was linearized by digestion with SmaI and ApaI (New England Biolabs) and purified by agarose gel electrophoresis ...
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bioRxiv - Synthetic Biology 2019Quote: All digestions were performed for 1-2h at 37° with REs purchased from NEB, and purified using the Monarch PCR & DNA Cleanup Kit (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... Spacer sequences were cloned into pXR003 by BbsI digestion and Gibson assembly (NEB, #E2611) with synthesized DNA duplexes containing the spacer sequence flanked by 19 bp homology arms (Integrated DNA Technologies (IDT)) ...
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bioRxiv - Cell Biology 2020Quote: ... and digestion mix was added (375 U DpnII [NEB] in 1x DpnII buffer [NEB]) and cells incubated for 16 h at 37 °C under rotation ...
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bioRxiv - Microbiology 2021Quote: ... removal of N-glycans was performed by digestion with PNGaseF (New England Biolabs, P0704S), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... enzymatic digestion was performed 30 min with 1 unit T7 endonuclease (New England Biolabs) at 37 °C ...