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Citations for New England Biolabs :
501 - 550 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: Cells were resuspended in 400 μl of restriction enzyme (RE) digestion mix (1x T4 ligase buffer (NEB, B0202S), 500U MseI (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... a Thioredoxin-tag (Trx) and a TEV cleavage site via restriction digestion (NcoI/XhoI, New England BioLabs®) and subsequent ligation (T4 DNA Ligase ...
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bioRxiv - Cancer Biology 2023Quote: We then set up the digestion reaction (1.5 μg of pX459, 2 μL of 10X NEBuffer 2.1, 1 μL of BbsI (NEB), and added H2O to a final volume of 20 μL) ...
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bioRxiv - Genomics 2023Quote: ... was subcloned into the pIRES2-dsRED2 plasmid using PCR with ag409 and ag410 and NotI restriction digestion (NEB) to create KCNE1-HA:pIRES2:dsRED2 ...
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bioRxiv - Genomics 2023Quote: ... nuclei were isolated from fixed B cells and subjected to in situ digestion using 200U MboI (NEB, R0147M) for 4 hours at 37 ºC ...
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bioRxiv - Molecular Biology 2023Quote: ... We obtained the right homology arm fragment by BamHI-HF®- and NotI-HF®-digestion (both NEB) and ligated it into the likewise BamHI-HF®- and NotI-HF®-digested HAL-pdTK-spacer-TK-mCherry-pA plasmid ...
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bioRxiv - Biochemistry 2023Quote: ... and then subcloned into the pCAG vector using either restriction digestion or Gibson assembly (New England Biolabs enzymes). ATG14 ...
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bioRxiv - Genetics 2023Quote: ... 1.2 M sorbitol) and resuspended in digestion buffer (Buffer B, 200 mM Vanadyl ribonucleoside complex [VRC from NEB] ...
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bioRxiv - Biochemistry 2024Quote: ... the larger DNA fragment of pNLX.Luc.R-.ΔAvrII73 following digestion with PflMI and AgeI restriction enzymes (New England Biolabs) was assembled with 1.85-kb synthetic fragments (Twist Bioscience ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified and filtrated RNA was then digested to single nucleoside by Nucleoside Digestion Mix (M0649S, NEB, Ipswich, MA). LC-MS/MS analysis was performed by injecting digested stable isotope labeled RNAs on an Agilent 1290 Infinity II UHPLC equipped with a G7117A diode array detector and a 6495C triple quadrupole mass detector operating in the positive electrospray ionization mode (+ESI) ...
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bioRxiv - Genetics 2023Quote: N-terminal GST-fusion TF DBD proteins were expressed using the PURExpress in vitro transcription/translation kit (NEB) following the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2023Quote: ... Selected candidates were expressed in vitro using the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Cell-free transcription and translation reactions were carried out using PURExpress® In Vitro Protein Synthesis Kit (NEB) following the manufactureŕs protocol ...
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bioRxiv - Biochemistry 2022Quote: ... protein was treated overnight with Lambda Protein Phosphatase (NEB #P0753S) at 4 °C.
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bioRxiv - Molecular Biology 2020Quote: ... 40 μg/ml phenylmethylsulfonyl fluoride and protease inhibitor) and resuspended again in 1 ml MNase digestion buffer with 1,250 Units MNase (NEB Biolabs). Chromatin-MNase mix was incubated at 37 °C for 30 min ...
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bioRxiv - Genetics 2021Quote: ... 1μl XhoI digestion mix consisting of 5U XhoI restriction enzyme and 1 x NEB buffer 2.1 (New England Biolabs), and 10 ng genomic DNA for iciHHV-6B samples or 200 ng DNA for non-iciHHV-6B samples ...
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bioRxiv - Biochemistry 2019Quote: ... Target plasmids and empty pUC19 were linearized by restriction enzyme digestion using BsaI-HF and nicked using a nicking enzyme Nt.BspQI (New England Biolabs). All restriction digestion and Gibson Assembly reactions were carried out as per the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2020Quote: ... target plasmids and empty pUC19 were linearized by restriction enzyme digestion using BsaI-HF and nicked using a nicking enzyme Nt.BspQI (New England Biolabs). All restriction digestion reactions were carried out as per the manufacturer’s protocols ...
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bioRxiv - Biophysics 2021Quote: Polymerised gels were removed from the coverslip chamber and placed into 6-well plates to undergo digestion in 0.2 mg/ml proteinase K (New England Biolabs) dissolved in digestion buffer (50 mM Tris pH 8.0 (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2021Quote: ... non integrated plasmids were cut by 2 h digestion at 37 °C with I-CeuI restriction enzyme (NEB, R0699S) in a total volume of 70 ul followed by 20 minutes heat inactivation at 65 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified small RNA samples were then digested using Nucleoside Digestion Mix (catalog no. M0649S, New England BioLabs, Beverly, MA). LC-MS/MS measurement of various post-transcriptional modifications were performed on a Waters XEVO TQ-STM (Waters ...
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bioRxiv - Genomics 2022Quote: ... Next, 500 nL of MspJI digestion mix (1x NEBuffer 4, 8x enzyme activator solution, 0.1 U MspJI (NEB, R0661L)) was added to each well and the plates were incubated at 37°C for 4.5 hours ...
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bioRxiv - Genomics 2022Quote: ... and overnight digestion was performed using 200 U of DpnII at 37 °C with shaking (New England Biolabs, R0543L) in NEB buffer 3.1 ...
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bioRxiv - Molecular Biology 2021Quote: ... ligated samples were decross-linked and subjected to a second round of digestion using the restriction enzyme CviQI (NEB). Digested samples were then ligated using T4 DNA ligase and purified before being used as template for inverse PCR ...
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bioRxiv - Microbiology 2020Quote: ... The NAT1 insertion construct was isolated from DH3261 by digestion with KpnI-HF and SalI-HF (New England BioLabs).
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bioRxiv - Genetics 2022Quote: ... the sgRNA sequences were inserted into the pBSMVγPDS plasmid after removing the PDS fragment by PacI and NotI digestion (New England BioLabs, Ipswich ...
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bioRxiv - Plant Biology 2019Quote: ... Plasmids used in this study were linearised by digestion using the appropriate restriction enzymes (New England Biolabs, Hitchin, UK). All DNA constructs were verified by sequencing (Source Bioscience).
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bioRxiv - Genetics 2019Quote: ... we performed first digestion of the chromatin with 25 units of NlaIII restriction enzyme (New England Biolabs, Cat#R0125) on a rotator at 37°C for overnight ...
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bioRxiv - Bioengineering 2019Quote: ... The PCR product was inserted into pcDNA3.1 via 5’EcoRI/3’NotI restriction digestion and a standard ligation protocol (T4 DNA Ligase; New England BioLabs) to create pcDNA3.1-Ncadherin ...
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bioRxiv - Molecular Biology 2020Quote: ... all sequences between transposable elements of a p2T plasmid were removed by restriction digestion using AleI and EcoRI (NEB). The TetO-eGFP-HygroR cassette was created by amplifying each subunit individually from already excising constructs ...
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bioRxiv - Microbiology 2020Quote: pCrPV-3 and pCrPV-1A-DcDV-1A were linearized by digestion with BamHI-HF (New England Biolabs, Ipswich, Massachusetts). Wild-type and CrPV-DcDV RNA was prepared by in vitro transcription of 1 µg linearized plasmid using the mMESSAGE mMACHINE T7 transcription kit (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... F1 dumpy animals were isolated and genotyped by PCR with let-7_SEQ_F5/R5 primers followed by an analysis of the PCR product using restriction digestion using EcoRV (NEB, Cat:N3195) and Sanger sequencing as described in (Duan et al. ...
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bioRxiv - Biochemistry 2021Quote: ... nuclei from 4 x 107 of cells were resuspended in 0.5 ml of MNase digestion buffer and digested with 2400 U of MNase (NEB) in the presence of 3.3 mM CaCl2 and 0.2% Triton X-100 at 30°C for 12 min with vigorous shaking (1200 rpm) ...
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bioRxiv - Neuroscience 2022Quote: ... the DNA was first cut by mixing the plasmid with a 10x digestion buffer (NEBuffer; Biolabs; Ipswich, MA, USA), a 10x EcoRI restriction enzyme (Biolabs) ...
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bioRxiv - Neuroscience 2023Quote: ... This was confirmed in N1 heterozygous AtrxR245C/+ females after digestion of the wild-type allele using FspI (NEB R0135S) and gel purification of the uncut mutant band ...
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bioRxiv - Microbiology 2022Quote: ... The DNA was then digested using 1000 units of either MluCI or HpaII in 1× NEB1 digestion buffer (NEB) and 1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The pdxAoperon amplicon and pACYC184 was ligated after digestion using T4 DNA ligase according to the manufacturer’s protocol (New England Biolabs). The Gibson or ligated product was transformed into E ...
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bioRxiv - Developmental Biology 2023Quote: ... The reporter HLC plasmid (20ng μl-1) was digested by 0.5 U I-Sce enzyme in its adequate digestion buffer (NEB) for 30’ at 37°C prior to injections ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR products were inserted into pET28a using restriction digestion and ligation with EcoR 1 and Sal 1 (NEB) sites.
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bioRxiv - Cancer Biology 2023Quote: ... nuclei were isolated and permeabilized to allow digestion of the chromatin by the primary RE (DpnII, New England Biolabs). Upon dilution ...
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bioRxiv - Microbiology 2023Quote: ... This digestion was performed on 1-3 µg of the PCR2 product with 1 µL of exonuclease (NEB #M0262) at 37°C for 30 min before heat inactivation ...
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bioRxiv - Genomics 2022Quote: ... Nuclei were isolated and permeabilized to allow digestion of the chromatin by the primary RE (NlaIII, New England Biolabs). Chromatin fragments were then diluted and ligated before crosslinking reversion ...
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bioRxiv - Biochemistry 2023Quote: ... The extracted plasmid DNA was reconstituted in cacodylic acid (10 mM, pH 6.5) and subjected to linearization by HINDIII-HF restriction digestion (New England Biolabs). Following digestion ...
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bioRxiv - Cell Biology 2023Quote: ... followed by restriction digestion of HRas constructs and the pEGFPC1 vector using Xho1/EcoR1 enzymes (New England Biolabs Inc.). Ligation was set up using T4 DNA ligase (Takara Bio.) ...
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bioRxiv - Molecular Biology 2023Quote: ... and inserted into the pKH011-puro backbone by simultaneous digestion of the backbone with BbsI-HF (NEB, Cat# R3539S) and ligation with T4 DNA ligase (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by DpnII digestion in a total volume of 50 µl with 10U of DpnII (NEB; R0543). DNA was amplified by PCR with Taq polymerase using the following program ...
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bioRxiv - Genomics 2024Quote: ... 500 µl lysed cells were then transferred to a tube containing 4.5 ml digestion buffer (1% Triton X-100, 1x CutSmart buffer (New England Biolabs)) ...
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bioRxiv - Genomics 2024Quote: ... 30 µL of the eluted DNA samples were mixed with 7 µL of TSO digestion mix (3.5 µL UDG-DNA glycosylase and 3.5 µL UDG Buffer, NEB M0280S) and incubated at 37 ºC for 30 min ...
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bioRxiv - Genomics 2024Quote: ... Incompletely formed SMRTbell templates were removed by digestion with a combination of exonuclease III and exonuclease VII (NEB, MA). The qualification and quantification of the SMRTbell libraries were made on a Qubit fluorimeter (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... using the plasmid pSP73-JY0 as template (55) followed by digestion with the restriction enzyme MluI (New England Biolabs). Labelled handles were ligated with the central part with T4 DNA Ligase (New England Biolabs ...