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Citations for New England Biolabs :
551 - 600 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... template was generated from pCRII-TOPO-NEAT1_1 (kind gift from Archa Fox) by double digestion with BamHI/NcoI (NEB), PCR amplification using Phusion polymerase (NEB ...
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bioRxiv - Genomics 2024Quote: ... 500 µl lysed cells were then transferred to a tube containing 4.5 ml digestion buffer (1% Triton X-100, 1x CutSmart buffer (New England Biolabs)) ...
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bioRxiv - Genomics 2024Quote: ... 30 µL of the eluted DNA samples were mixed with 7 µL of TSO digestion mix (3.5 µL UDG-DNA glycosylase and 3.5 µL UDG Buffer, NEB M0280S) and incubated at 37 ºC for 30 min ...
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bioRxiv - Genomics 2024Quote: ... Incompletely formed SMRTbell templates were removed by digestion with a combination of exonuclease III and exonuclease VII (NEB, MA). The qualification and quantification of the SMRTbell libraries were made on a Qubit fluorimeter (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Cas9 protein (NEB) was mixed with all six sgRNAs and injected into embryos at the 1-cell stage ...
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bioRxiv - Plant Biology 2024Quote: ... SpCas9 protein (NEB), 1 x cut smart buffer (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1600 v/10 ms /3 pulses for 200,000 cells in Buffer R (Neon Transfection kit) premixed with 50 pmol Cas9 protein (CAT#M0646T, New England Biolabs), 50 pmol single guide RNA (sgRNA ...
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bioRxiv - Cell Biology 2022Quote: Recombinant C-terminally FLAG-Tagged TEX264 was produced by PURExpress in vitro protein synthesis kit (E6800; New England Biolabs). Phosphorylation reaction containing TEX264-FLAG and CK2 (mixture of CK2A1 and CK2B ...
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bioRxiv - Physiology 2021Quote: ... The biotinylated peptides were placed on streptavidin-coated plates and three rounds of solid-phase panning were conducted using 109 random 12-mer peptides fused to a minor coat protein of M13 (pIII) following the manufacture’s protocol (Ph.D-12 Phage Display Peptide Library Kit, NEB, E8110S). Sequenced data were subsequently analyzed as follows ...
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bioRxiv - Evolutionary Biology 2021Quote: ... see Supplementary Data 6) and cloned into pDHFR vector provided in the PURExpress In Vitro Protein Synthesis Kit (NEB). The translation into liposomes was done as described previously95 and the output was analyzed by Blue Native PAGE using 2% digitonin and NativePAGE Novex 4-16% Bis-Tris Protein Gel (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... 20 ng of each integrase amplicon was used per reaction using PURExpress® In Vitro Protein Synthesis Kit (NEB) in a total volume of 9 µl ...
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bioRxiv - Microbiology 2022Quote: ... Protein samples were then mixed with Blue Protein Loading Dye (NEB) boiled for 10 minutes and separated on 10% SDS-PAGE gel for 50 minutes at 140 V ...
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bioRxiv - Plant Biology 2021Quote: ... and extracted nuclear proteins were treated with lambda protein phosphatase (NEB) according to the manufacturer’s instruction.
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bioRxiv - Genomics 2020Quote: ... Endogenous proteins were captured onto protein G-magnetic beads (NEB; #S1430S), washed extensively in IP buffer and used for POT1wtand POT1V29L source ...
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bioRxiv - Molecular Biology 2020Quote: ... 40 μg/ml phenylmethylsulfonyl fluoride and protease inhibitor) and resuspended again in 1 ml MNase digestion buffer with 1,250 Units MNase (NEB Biolabs). Chromatin-MNase mix was incubated at 37 °C for 30 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Following the adaptor ligation each sample was mixed with 20 μl of a 2x DpnII Digestion Buffer and 10 units (1 μl) of DpnII restriction enzyme (New England Biolabs) mastermix and were incubated for 3 hours at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... 2010) (gift from David Stanek, Institute of Molecular Genetics, Czech Republic) by digestion with NheI and BamHI restriction endonucleases (NEB). The fragment was then directionally cloned into the NheI/BamHI sites downstream of the UAS motifs of the pT2 5UAS MCS eGFP construct generating C-terminally fused enhanced green fluorescent protein (hSNRNP70-eGFP) ...
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bioRxiv - Molecular Biology 2022Quote: ... The lenti-sgRNA puro vector was digested with EcoRI for 2h at 37°C followed by digestion with BsmBI for 2 hours at 55°C and treatment with 4 μl of rSAP (NEB) for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAse I digestion to analyse 2’-O-ribose methylation was done in the presence of 10 U T4 PNK (NEB) in 50 mM Tris-acetate (pH 6.5) ...
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bioRxiv - Genomics 2020Quote: ... Primary restriction enzyme digestion of intact nuclei was carried out overnight at 37°C using 50,000 U of DpnII (NEB: #R0543) with agitation at 900 RPM ...
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bioRxiv - Molecular Biology 2020Quote: ... were separately subjected to restriction enzyme double digestion at 37 °C overnight in 20 μL volumes containing 20 U BamHI-HF (cat. no. R3136S, New England BioLabs), 20 U EcoRI-HF (cat ...
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bioRxiv - Molecular Biology 2020Quote: ... aqueous phase containing the barcoded cDNA (~50 μL) was combined with 50 μL digestion mix containing 5 μL ExoI enzyme (NEB), 5 μL HinFI enzyme (NEB) ...
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bioRxiv - Cell Biology 2019Quote: ... This was sub-cloned into a (pUAS-k10.attB) plasmid using standard restriction digestion with NotI and BamHI (New England Biolabs) followed by ligation with T4 DNA ligase (New England Biolabs ...
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bioRxiv - Genomics 2019Quote: ... The supernatant was carefully removed and nuclei were resuspended in 100 μl of DNase digestion buffer (1× DNase buffer (NEB), 25 µM α-amanitin ...
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bioRxiv - Synthetic Biology 2020Quote: ... Integration into the yeast genome via homologous recombination at the URA3 or LEU2 locus was achieved by transformation of linearized plasmids (NotI digestion, NEB) whereas replicating CEN6/ARS4 or 2µ plasmids were transformed directly into yeast without pre-digestion with NotI ...
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bioRxiv - Genetics 2019Quote: An A/G polymorphism (rs25531) present in L variants was assayed in all amplification products by digestion with 24U of MspI (New England Biolabs) overnight at 37°C as recommended by Wang et al ...
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bioRxiv - Bioengineering 2019Quote: ... mycoplasma genomes are released by digestion of the agarose matrix with three units per plug of β-Agarase I (NEB), and the DNA concentration is measured using an Epoch™ Microplate Spectrophotometer (BioTek™).
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bioRxiv - Cancer Biology 2019Quote: ... Cas9 was PCR amplified and inserted into the multiple cloning site by digestion with EcoRI and BamHI followed by overnight ligation with T4 ligase (NEB).
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bioRxiv - Biophysics 2019Quote: ... Vector pBACgus11 (gift from Borja Ibarra) was linearized through double digestion using restriction enzymes SacI and BamHI (New England Biolabs, NEB). A dsDNA handle with digoxigenin (DIG ...
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bioRxiv - Molecular Biology 2020Quote: ... Subcloned cells were screened for correct targeting by PCR amplification and restriction enzyme digestion (MCM10 exon 3, Hpy199III (NEB R0622); CDC45 exon 3 ...
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bioRxiv - Biochemistry 2020Quote: ... These beads were then resuspended in equal amounts of PBS+ DNaseI digestion buffer with 3 ul (6U) of DNAseI (M0303S, NEB) and incubated at 37°C for 40min - 1h and separated by centrifuging at 2000 rpm for 2 min ...
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bioRxiv - Biochemistry 2020Quote: ... Trypsin digest of PDI was followed by digestion of N-glycans with endo-β-N-acetylglucosaminidase H (500 U; NEB) in sodium citrate buffer (50 mm ...
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bioRxiv - Biochemistry 2021Quote: ... The guide RNA target site was amplified from transfected cells and analyzed by T7 Endonuclease I digestion of re-annealed PCR products (New England Biolabs). The guide RNA eventually selected for genome editing in embryos was Bahd1-sg47B (protospacer sequence 5’-GCCTGTAATACCACAGG −3’) ...
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bioRxiv - Biochemistry 2021Quote: ... Primer pairs were cloned by double digestion using SacI and SalI restriction enzymes (R0156, R0156, CutSmart Buffer, New England Biolabs) and ligation with T4 DNA Ligase (EL0014 ...
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bioRxiv - Biochemistry 2020Quote: ... the truncated CNNM2 PCR products were cloned into the pCINE-IRES-GFP vector by digestion with restriction enzymes NheI (New England Biolabs) and XhoI (New England Biolabs).
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bioRxiv - Cell Biology 2021Quote: ... pRS316 (Sikorski and Hieter, 1989), p424GPD, or p426GPD (Mumberg et al., 1995) through restriction enzyme digestion and T4 DNA ligase-mediated ligation (NEB). The resulting plasmids were verified by enzymatic digestion and DNA sequencing ...
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bioRxiv - Plant Biology 2020Quote: ... and uvr8-17D genotyping was through PCR amplification of a genomic fragment with a forward (5ʹ-TCG GGA TGA GAT GAT GAC-3ʹ) and a reverse primer (5ʹ-TAG ACC CAA CAT TGA CCC-3ʹ) followed by digestion with HaeIII (NEB) yielding diagnostic fragments of 344/173/145 bp for wild type and 489/173 bp for uvr8-17D.
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bioRxiv - Microbiology 2021Quote: ... PCR products containing the alanine mutation were cloned under the control of the ara promoter by insertion into pBAD33 by digestion with Kpn1 (NEB) and Sph1 (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... 6 µg of DNA was used for MmeI digestion in 200 µL (6 µg gDNA, 6 µL MmeI (2000 U/mL, NEB), 0.5 µL 32 mM S-adenosylmethionine ...
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bioRxiv - Biophysics 2020Quote: The central part of the DNA construct used for magnetic tweezers was a 7.9 kbp fragment produced by restriction digestion of λ-DNA using SapI and BsaI (NEB) and subsequent agarose gel purification ...
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bioRxiv - Molecular Biology 2022Quote: ... 2019 [36] with the modification that 3 µg of gDNA per technical replicate were digested using Nucleoside Digestion Mix (NEB M0649S ...
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bioRxiv - Plant Biology 2022Quote: ... the SYLF domain was mutagenized by ordering a synthetic DNA sequence carrying point mutations (Table 4) and replaced by restriction enzyme digestion with NcoI (NEB) and XbaI (NEB) ...
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bioRxiv - Bioengineering 2022Quote: ... The GC3-optimized NPC1 sequence was encoded in a plasmid (Twist Bioscience) and linearized via double restriction enzyme digestion using BamHI-HF (NEB) and HindIII-HF (NEB) ...
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bioRxiv - Cancer Biology 2022Quote: ... we used Addgene Plasmid #48140 as a transient expression vector backbone by removing Cas9 and GFP with EcoRI-AgeI digestion (NEB), and inserting three overlapping gBlock dsDNA fragments for JAK1 ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was subjected to λ exonuclease digestion by incubating the beads with 50 μL of λ exonuclease reaction buffer (NEB) containing 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2021Quote: ... Library preparation began with the digestion of 1pg–200 ng genomic DNA in a 15-µl reaction using 4 U BcgI (NEB) at 37 °C for 3 h ...
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bioRxiv - Biochemistry 2020Quote: ... NC-siRNA sense: 5’-AGGUAGUGUAAUCGCCUUGdTdT-3’.35,36 NEBuffer™ 2 and nucleoside digestion mix were obtained from NEB (Ipswich, MA). Adenosine and N6-methyl adenosine (m6A ...
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bioRxiv - Molecular Biology 2020Quote: ... Genotyping was performed with PCR amplifying 253bp across the cutting sites and looking for acquired resistance to MspA1l digestion (NEB). Positive clones were further confirmed by immunoblotting ...
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bioRxiv - Plant Biology 2022Quote: RNase If digest was performed with 2,000 relative absorbance units OD260 of the in RNase digestion buffer suspended polysomes by adding 50 U of RNase If (New England Biolabs) in 250 µL total volume by incubation at room temperature with constant rotation for 1 h ...
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bioRxiv - Plant Biology 2019Quote: ... the amplicon was then blunt-end ligated into the AM439 destination clone linearized with the PmeI restriction digestion enzyme (NEB).