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Citations for New England Biolabs :
301 - 350 of 6954 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 10 μL of WarmStart Colorimetric Lamp 2X Master Mix (M1800) (New England Biolabs, USA), 5 μL of DNAse ...
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bioRxiv - Microbiology 2020Quote: ... PCRs were amplified using Q5® High fidelity DNA polymerase 2X master mix (NEB), 25 pmol of specific ultramers and 20 ng of DNA template ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... Colony PCR was performed using Q5 High-Fidelity 2X Master Mix (New England Biolabs) and the hACE2 insert primers to identify a positive colony ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR indexing was performed using NEBNext High-Fidelity 2x PCR Master Mix (M0541S, NEB) and sequenced using a NextSeq platform (Illumina) ...
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bioRxiv - Molecular Biology 2021Quote: ... amplicons were generated using Q5 Hot Start High-Fidelity 2x Master Mix (M0494, NEB). Amplicons for long-read sequencing were generated with Q5 High-Fidelity polymerase (M0492S ...
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bioRxiv - Microbiology 2021Quote: ... 7.5 μl of OneTaq Quick-Load 2X master mix (New England Biolabs, cat#M0486), and milliQ water to 15 μl ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl of 2X Blunt/TA Ligase master mix (New England Biolabs, cat#M0367S), and milliQ water up to 10 μl total volume ...
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bioRxiv - Plant Biology 2021Quote: ... PCR were performed with Quick-Load® Taq 2x Master Mix (New England BioLabs) according to the manufacturer recommendations with the primer displayed in Table S3 ...
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bioRxiv - Cell Biology 2021Quote: ... the purified DNA and the NEBNext® High-Fidelity 2X PCR Master Mix (NEB). PCR reactions were pooled and purified using the QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Genetics 2022Quote: ... NEBNext® High-Fidelity 2X PCR Master Mix (New England BioLabs, catalog number M0541) and SybrGreen were used to assess the expression level of the Q gene on chromosome 5A ...
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bioRxiv - Synthetic Biology 2022Quote: Q5 High Fidelity 2X Master Mix (New England Biolabs GmbH [NEB], Germany, No. M0492S) was used to perform DNA amplification ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR was performed using Hot Start Taq 2X Master Mix (New England Biolabs, USA) in a 20 μL reaction mixture containing 0.25 μM of each primer ...
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bioRxiv - Genomics 2019Quote: ... 1 × SYBR Green and 5μl NEBNext High-Fidelity 2X PCR Master Mix (NEB M0541S), to estimate the optimum number of enrichment cycles with the following program ...
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bioRxiv - Bioengineering 2019Quote: ... Full-length oligonucleotides were PCR-amplified using Q5 High-Fidelity 2X Master Mix (NEB), size-selected using a 3% agarose EGel EX (Life Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were amplified by PCR using NEBNext High-Fidelity 2x PCR Master Mix (NEB) using PCR primers Ad1_noMX and Ad2_Barcode (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2020Quote: ... Vector PCRs were performed using Q5 High-Fidelity 2X Master mix (New England Biolabs). All GFP related libraries were cloned using BsaI sites ...
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bioRxiv - Cell Biology 2021Quote: ... cDNAs were subjected to end-point PCR using OneTaq® 2X Master Mix (NEB) or quantitative PCR (qPCR ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCRs were performed using either: (i) the Q5 High-Fidelity 2x Master Mix (NEB), followed by DpnI (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR was performed with Hot Start Taq 2X Master Mix (New England Biolabs Inc) in a 20 μL reaction mixture containing 1.5 mM of MgCl2 ...
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bioRxiv - Microbiology 2020Quote: ... plantarum B21 plasmids using OneTaq® 2X Master Mix with Standard Buffer (NEB, USA) in a T100™ Thermal Cycler (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... Two rounds of PCR were performed using Q5 High-Fidelity 2X Master Mix (NEB). For the first round of PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... The library was amplified using NEBNext High-Fidelity 2X PCR Master Mix (NEB; M0541S) and cleaned using SPRI-beads with left-side size selection protocol ...
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bioRxiv - Microbiology 2021Quote: ... Each PCR included 12.5 μL Q5 High Fidelity 2x Master Mix (New England Biolabs), 3.6 μL of either pool 1 or pool 2 10μM primer master mix (final concentration of each primer was ~10-11pM) ...
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bioRxiv - Genomics 2022Quote: ... then 4 μl of Q5 High-Fidelity 2X Master Mix (NEB, cat. no. M0541L) was added to each well ...
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bioRxiv - Biochemistry 2022Quote: ... PCR fragments were amplified using Q5 High-Fidelity 2x Master Mix (New England Biolabs). The primers (IDT ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR was carried out with Q5 High-Fidelity 2X Master Mix (New England BioLabs) using the conditions recommended by the manufacturer for 35 cycles ...
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bioRxiv - Genetics 2022Quote: ... 30µL of Phusion High Fidelity 2x Master Mix (New England Biolabs, Ipswich MA, USA), 3µL of 10 µM each of P1 and P2 primers (Table S1 ...
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bioRxiv - Synthetic Biology 2022Quote: Q5 High Fidelity 2X Master Mix (New England Biolabs GmbH [NEB], Germany, No. M0492S) was used to perform DNA amplification ...
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bioRxiv - Synthetic Biology 2022Quote: ... and amplified using Q5 Host-Start High-Fidelity 2x Master Mix (New England Biolabs). PCR products were then size selected to remove adaptor-dimers below 200 bp using three subsequent size-selection rounds with a Select-a-Size DNA Clean & Concentrator Kit (D4080 ...
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bioRxiv - Bioengineering 2023Quote: ... DNA fragments were amplified by PCR (Q5 2x Master Mix, New England Biolabs (NEB)) ...
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bioRxiv - Genomics 2023Quote: ... DNA was amplified by PCR with NEBNext HiFi 2x PCR Master Mix (NEB M0541S) and library was purified by 1.3X SPRI purification (Omega Bio-Tek ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was PCR-amplified using Q5 High-Fidelity 2x Master Mix (NEB M0492) for 20 cycles ...
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bioRxiv - Microbiology 2023Quote: DNA was amplified by Q5® High-Fidelity 2X Master Mix (New England Biolabs) in a 25 μl reaction ...
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bioRxiv - Genomics 2023Quote: ... Libraries were amplified using NEBNext HiFi 2X PCR Master mix (NEB cat. no. M0541) with 13 rounds of amplification as described previously28 ...
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bioRxiv - Genomics 2022Quote: ... 25 μl of NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs, M0541S), 2.5 μL each of Nextera i5 and i7 indexed amplification primers (Nextera Index Kit ...
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bioRxiv - Molecular Biology 2023Quote: ... the first round of PCR was performed using LongAmp Taq 2X master mix (NEB) and 2 – 3 uL of cDNA (1:5 diluted in water ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cloning fragments were PCR amplified using Q5 polymerase 2X master mix (New England Biolabs). Full construct sequencing was performed by Plasmidsaurus (Eugene ...
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bioRxiv - Pathology 2023Quote: ... containing 6.25 μL of Taq 2x Master Mix (New England Biolabs, Ipswich, MA, U.S.A), 0.16 μL of the forward primer with the M13 tail ...
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bioRxiv - Immunology 2024Quote: ... Amplification reactions consisted of 50 μL LongAmp Taq 2x Master Mix (New England Biolabs), 1 μL 10 μM forward primer ...
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bioRxiv - Immunology 2024Quote: ... and PCR amplified with NEBNext High Fidelity 2x PCR Master Mix (New England Biolabs). Following purification with the PCR Purification Kit (QIAGEN) ...
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bioRxiv - Cancer Biology 2023Quote: ... sgRNA inserts were amplified with NEBNext High-Fidelity 2X PCR Master Mix (NEB M0541L). Samples were then pooled in equimolar concentrations ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR was performed using NEBNext® High-Fidelity 2X PCR Master Mix (NEB; M0541S). The purified PCR products were separated by a 4-20% TBE gel (Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: Polymerase Chain Reaction (PCR) was performed using Q5 High Fidelity 2X Master Mix (NEB) with primers synthesized by Integrated DNA Technologies (IDT ...
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bioRxiv - Biochemistry 2024Quote: ... Correct assembly was verified by PCR using Taq 2X Master Mix (New England BioLabs) and Sanger sequencing (Genewiz).
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bioRxiv - Developmental Biology 2023Quote: ... ∞ at 4 °C) using NEBNext HighFidelty 2x PCR Master Mix (New England Biolabs, M0541S) and Illumina i5 and i7 indices54 ...
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bioRxiv - Genetics 2023Quote: ... along with an additional 5 µL 2x Gibson Assembly Master Mix (NEB Cat#E2611L). The reaction mixture was incubated at 50°C for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: PCR was performed using Q5 High Fidelity 2X Master Mix (New England Biolabs, M0492) with the following temperature and times.
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bioRxiv - Cancer Biology 2024Quote: ... CUT&Tag libraries were prepared with NEBNext HiFi 2x PCR Master Mix (NEB M0541S) and indexed primers (Buenrostro et al. ...
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bioRxiv - Immunology 2023Quote: ... and amplified using NEBNext® High-Fidelity 2x PCR Master Mix (NEB, Cat. #M0541s) for 9 cycles ...
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bioRxiv - Immunology 2023Quote: ... samples were amplified with NEBNext High Fidelity 2x PCR Master Mix (New England Biolabs) (Buenrostro et al ...