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Citations for New England Biolabs :
251 - 300 of 6954 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... 25 μL NEBNext Hi-Fi 2x PCR Master Mix (NEB (Ipswich, MA, USA) M0541S) ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCRs were performed using Q5® High-Fidelity 2X Master Mix (NEB #M0492L), sfGfp as the template ...
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bioRxiv - Genetics 2021Quote: ... PCR amplicons were generated using Q5® High-Fidelity 2X Master Mix (NEB) using primers PKS12_F2 and PKS12_R2 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then amplified twice using 2x Q5 Master Mix (New England BioLabs) and two unique primer sets ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR reactions were performed with Taq 2X Master Mix (New England BioLabs, M0270L). The thermocycler conditions for Mouse Chk1 and GAPDH PCR product were as follows ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA (20 ng) was PCR amplified using LongAmpTaq 2X master mix (NEB). The PCR amplicons of selected regions were subjected to Sanger sequencing and BLAST analysis to confirm the presence of eight variants using the primers listed in Supplemental Table 1.
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bioRxiv - Genetics 2020Quote: ... 50 μl LongAmp Taq 2x master mix (New England Biolabs, Ipswich, MA, USA). Initial denaturation was for 3 min at 95 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Q5-high Fidelity 2X master mix was used as polymerase from NEB (# M0429L). PCR condition for the first PCR was ...
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bioRxiv - Developmental Biology 2022Quote: ... Libraries were prepared using NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541) with the following conditions ...
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bioRxiv - Cell Biology 2022Quote: ... The first reaction with Q5 Hot Start High-Fidelity 2X Master Mix (NEB) amplifies the gRNA cassette (primers #1 and #2 for CRISPRi and small pooled screen libraries ...
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bioRxiv - Synthetic Biology 2023Quote: ... from PCR fragments obtained with Q5 High-Fidelity 2X Master Mix (#M0492L, NEB). eolTRPA1 coding sequence from Texas Rat snake (Elaphe (Pantherophis ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1x of OneTaq Hot Start 2X Master Mix with Standard Buffer (NEB, M0484L), and 1x Chai Green Dye (CHAI ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1x of OneTaq Hot Start 2X Master Mix with Standard Buffer (NEB, M0484L). The qPCR was performed on CFX96 Touch Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... Samples were then amplified twice using 2x Q5 Master Mix (New England BioLabs) and two unique primer sets ...
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bioRxiv - Evolutionary Biology 2022Quote: ... using 25 ul of Q5 Hot Start Master Mix (2X) (New England Biolabs) for a total volume reaction of 50 ul.
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bioRxiv - Evolutionary Biology 2022Quote: ... using 25 ul of Q5 Hot Start Master Mix (2X) (New England Biolabs) for a total volume reaction of 50 ul.
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A laboratory framework for ongoing optimisation of amplification based genomic surveillance programsbioRxiv - Genomics 2023Quote: ... Each PCR contained 12.5μL Q5 High Fidelity 2x Master Mix (New England Biolabs), 1.1μL of pooled primers mixes at 10μM (final concentration of each primer was ∼10-11pM) ...
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bioRxiv - Microbiology 2023Quote: ... Linearized pExTra plasmid was prepared via PCR (NEB Q5 HotStart 2X Master Mix) of pExTra01 (Heller et al ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA PCR was performed with using OneTaq® 2X Master Mix (NEB) and used primer pairs were ...
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bioRxiv - Evolutionary Biology 2023Quote: ... All PCR mixtures used Q5 Hot Start High-Fidelity 2X Master Mix (NEB) with the specified primers and templates below ...
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bioRxiv - Genomics 2023Quote: ... The PCR was performed using Q5 High-Fidelity 2X Master Mix (NEB, M0492L) with conditions described in Supplementary Table 4.
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bioRxiv - Biophysics 2023Quote: ... and Q5 Hot Start High-Fidelity 2X PCR Master Mix (New England Biolabs). The transcribed region had the following spacings ...
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bioRxiv - Plant Biology 2022Quote: ... using a LongAmp Hot Start Taq 2x Master Mix (M0533S, New England Biolabs), and 16S barcoded primers.
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bioRxiv - Genetics 2023Quote: ... 10 μl of Q5 Hot Start High-Fidelity 2x Master Mix (NEB M0494S), 1 μl of 10 μM Primer 1 (5’-ATGAAGCTACTGTCTTCTATCGAACAAGC-3’ ...
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bioRxiv - Cell Biology 2024Quote: ... and PCR was performed using Q5® High-Fidelity 2X Master Mix (NEB). The splicing efficiency is monitored using RT-PCR with primers located on two adjacent exons ...
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bioRxiv - Microbiology 2023Quote: ... 25 µl NEBNext High-Fidelity 2X PCR Master Mix (NEB, catalog number M0541S). In each 50 µl reaction ...
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bioRxiv - Microbiology 2023Quote: ... PCR was performed using the Phusion Hot Start Flex 2X Master Mix (NEB) in a GeneAmp PCR system 9700 (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... and amplified and indexed with NEBNext High- Fidelity 2X PCR Master Mix (NEB) and custom primers ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... coli DH5alpha colonies using OneTaq® 2X Master Mix (New England Biolabs, USA) to confirm the correct insertions and further sequenced with Sanger method (Eurofins Scientific ...
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bioRxiv - Microbiology 2023Quote: PCR was performed using Q5 High-Fidelity 2X Master Mix (New England Biolabs) as per manufacturer’s instructions with the following primers ...
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bioRxiv - Developmental Biology 2024Quote: ... and screened by colony PCR with OneTaq Quick-Load 2X Master Mix (NEB). Positive colonies were cultured overnight (o/n ...
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bioRxiv - Neuroscience 2024Quote: ... and Q5 High-Fidelity 2X Master Mix (New England Biolabs, Ipswich, MA, USA). RT-PCR protocol was involved in 35 cycles of PCR at 98°C for 10 sec ...
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bioRxiv - Genomics 2024Quote: ... mixed with 50 µL 2x NEBuilder HiFi DNA assembly Master mix (NEB E2621L) and incubated at 50 °C for 2 hr ...
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bioRxiv - Developmental Biology 2022Quote: ... NEBNext Ultra II Q5 Master Mix (M0544S, New England BioLabs) and primers (Supplementary Table 8 ...
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bioRxiv - Genomics 2020Quote: ... 10 µl of NEBNext Ultra II Q5 Master Mix (NEB) then immediately amplified in a thermocycler under the conditions ...
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bioRxiv - Genomics 2021Quote: ... For Smartseq3xpress with NEBNext Ultra II Q5 Master Mix (NEB), PCR mastermix consisted of (1x NEBNext Ultra II Q5 Master Mix ...
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bioRxiv - Synthetic Biology 2024Quote: ... using the NEBNext Ultra II Q5 Master Mix (NEB, #M0544), primers (0.5 μM each ...
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bioRxiv - Plant Biology 2019Quote: ... PCR amplification was then carried out in a 25μl reaction volume using 2X Hot Start Taq 2X Master Mix (NEB) according to the manufacturer’s recommendations and using the following primers: from Eurofins Genomics (Ebersberg ...
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bioRxiv - Synthetic Biology 2024Quote: All PCRs were carried out using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB), gel extractions using the QIAquick Gel Extraction Kit (QIAGEN ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCRs were carried out using the Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB) in a total volume of 20ul ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR was performed using the OneTaq-Quick-Load 2X Master Mix (New England BioLabs) with Aedes whole-body cDNA as a template ...
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bioRxiv - Molecular Biology 2021Quote: ... We also tested NEB Q5 Hot Start High-fidelity 2x Master Mix (NEB #M0494L), NEB Q5 Hot Start High-fidelity 2x Master Mix plus .01% SDS ...
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bioRxiv - Immunology 2021Quote: ... OneTaq 2x Master Mix with Standard Buffer was used (New England BioLabs, Ipswich, MA). As a template in the first round ...
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bioRxiv - Immunology 2021Quote: ... OneTaq 2x Master Mix with Standard Buffer was used (New England BioLabs, Ipswich, MA). As a template in the first round ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR amplification was carried out using Q5 High-Fidelity 2X Master Mix (NEB, M0492L) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: Darwin Assembly master mix (2x): 0.05 U/µl Q5 High-Fidelity DNA polymerase (NEB), 8 U/µl Taq DNA ligase (NEB) ...
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bioRxiv - Genomics 2020Quote: ... Libraries were amplified using NEBNext® High-Fidelity 2X PCR Master Mix (#M0541S, NEB) with primers Ad1 ...
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bioRxiv - Immunology 2021Quote: ... 25 μl NEBNext High-Fidelity 2x PCR Master Mix (New England BioLabs, Cat. #M0541L) per reaction and amplified via a 6-cycle PCR program (1 cycle of 72°C for 5 min ...
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bioRxiv - Neuroscience 2021Quote: ... Three rounds of nested PCR using Phusion HF 2x Master Mix (New England Biolabs) were used to obtain highly specific amplicons for the UNC13A cryptic ...
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bioRxiv - Microbiology 2021Quote: ... Secondary amplification reactions were performed using NEBNext High-Fidelity 2X PCR Master Mix (NEB) in 50 µL reaction (26 µL of 2X Mix ...