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Citations for New England Biolabs :
4701 - 4750 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Sequencing libraries were generated using NEBNext® Ultra TM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Cell Biology 2022Quote: ... then libraries were generated using NEBNext® Ultra TM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2021Quote: ... the cDNA libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (E7530S, New England Biolabs). Quality of the cDNA library was also assessed by Qubit assay for concentration ...
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bioRxiv - Biochemistry 2021Quote: ... The E PBM C-terminal mutation (DVLL>GGGG) was performed using the Q5 site-directed mutagenesis kit (NEB). The sequences corresponding to the PDZ domains were cloned into the pCMV GFP vector using EcoRI and XhoI sites.
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bioRxiv - Microbiology 2021Quote: ... The two amplicons were fused using a NEBuilder HiFi DNA Assembly kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcription and qRT-PCR analysis was performed by Luna universal one- step qRT-PCR kit (NEB # E3005L) with primers list in Table 9.
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... as was the pCDH vector before purification again with Monarch® DNA Gel Extraction Kit (New England Biolabs). The ACE2 fragment was ligated with pCDH vector using T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Developmental Biology 2021Quote: ... and cDNA was generated by reverse transcription using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB). cDNA was amplified with primers flanking each splice site by Taq polymerase (NEB ...
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bioRxiv - Microbiology 2020Quote: ... Sequencing libraries were generated using NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB, USA). PCR was performed with Phusion High-Fidelity DNA polymerase ...
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bioRxiv - Molecular Biology 2020Quote: ... library preparation was completed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). The adapters and PCR primers used were purchased from New England BioLabs ...
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bioRxiv - Molecular Biology 2020Quote: ... strand-specific cDNA libraries were generated by NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... We constructed libraries from 300 ng of RNA using NEB Next Ultra RNA kit (New England Biolabs, USA) and isolated Poly(A ...
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bioRxiv - Microbiology 2020Quote: ... Whole genome shotgun libraries were prepared using the NEB Ultra Library Prep kit (New England Biolabs; Ipswich, MA) for sequencing on either the MiSeq or HiSeq (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: Library preparation: TrAEL-seq adaptor 2 was added using a modified NEBNext Ultra II DNA kit (NEB E7645S): 3.5 μl NEBNext Ultra II End Prep buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... First strand cDNA was synthesised using a ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, E6560) using oligo d(T)23 primers and 500 ng total RNA.
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bioRxiv - Microbiology 2020Quote: ... using primers targeting the E gene of SARS-CoV-2 (E_Sarbeco-F ACAGGTACGTTAATAGTTAATAGCGT; E_Sarbeco-R ATATTGCAGCAGTACGCACACA) and Luna Universal One-Step qRT-PCR Kit (New England Biolabs) on a Roche Light Cycler 480 ...
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bioRxiv - Neuroscience 2021Quote: ... Libraries were prepared according to the “NEBNext Ultra Directional RNA Library Prep kit for Illumina” (New England Biolabs) instructions and sequenced using a “NextSeq™ 500 High Output Kit” ...
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bioRxiv - Microbiology 2021Quote: ... and dual indexed libraries were prepared using the NEBNext Ultra Directional RNA sequencing kit (New England Biolabs, USA). Libraries were sequenced on a HiSeq 4000 (Illumina ...
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bioRxiv - Biochemistry 2021Quote: The VoC RBD mutations were introduced by site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs). Successful cloning was confirmed by Sanger sequencing (Genewiz ...
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bioRxiv - Microbiology 2020Quote: Site-directed mutagenesis was performed using the Q5 site-directed mutagenesis kit according to the manufacturer’s instructions (NEB). All mutations were confirmed by DNA sequencing.
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bioRxiv - Immunology 2020Quote: ... Restriction enzymes (BamHI-HF and NheI-HF) and Quick Ligation kit were purchased from New England Biolabs (NEB). Linker insertion and modifications in the antigen-bearing components were achieved using Q5 Site Directed Mutagenesis Kit (NEB) ...
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bioRxiv - Immunology 2020Quote: ... Linker insertion and modifications in the antigen-bearing components were achieved using Q5 Site Directed Mutagenesis Kit (NEB). Custom DNA primers produced by Integrated DNA technologies (IDT) ...
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bioRxiv - Cancer Biology 2020Quote: ... in vitro transcription with T7 polymerase was done by T7 high yield RNA systhesis kit (New England Biolabs). Purification of mRNA was done by Qiagen mini column ...
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bioRxiv - Cancer Biology 2020Quote: ... mRNA libraries were prepared using the NEB Next Ultra II Directional RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA library was constructed according to the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, E7530) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Neuroscience 2021Quote: ... Generation of the CK2K198R mutant was done using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs).
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bioRxiv - Microbiology 2021Quote: ... followed by library preparation with NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs). DNA library preparation included 6 cycles of PCR amplification ...
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bioRxiv - Microbiology 2021Quote: ... Catalytically inactive Mt2 (Mt2 C78A) variant was generated via site-directed mutagenesis (NEB, Q5 Site-Directed Mutagenesis Kit) using primers listed in the primer table (Table S1).
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bioRxiv - Biochemistry 2020Quote: ... The G163H (30) and G163D variants were constructed using the Q5 site-directed mutagenesis kit (New England Biolabs) to introduce the mutation ...
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bioRxiv - Molecular Biology 2022Quote: ... Strand-specific libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). Libraries were sequenced by paired-end sequencing with a 75 (patient samples ...
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bioRxiv - Neuroscience 2022Quote: ... sequencing libraries were generated using a NEBNext Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs) and index codes were added to attribute sequences to each sample ...
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bioRxiv - Neuroscience 2022Quote: ... One-step RT-qPCR was performed using the Luna universal One-Step RT-qPCR kit (New England BioLabs) in a total volume of 20 µl and a template concentration of 50 ng/µl according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... High molecular weight genomic DNA extractions were prepared using the Monarch HMW DNA Extraction Kit for Tissue (NEB). All 14 isolates cultured from DFU141 were initially sequenced with paired-end sequencing on Illumina HiSeq 2500 at PennCHOP Microbiome Core ...
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bioRxiv - Microbiology 2022Quote: ... The synthesized ssDNA was purified using a Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA, USA) and was eluted in ultrapure water ...
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bioRxiv - Systems Biology 2022Quote: ... Strand-specicific libraries were created using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, #E7760). Final libraries were quantified using qPCR and clustered at a Molarity of 300 pM ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The mRNA libraries were constructed with NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer’s protocol to prepare 300-bp RNA fragments for 150 bp paired-end (PE150 ...
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bioRxiv - Immunology 2022Quote: ... The qPCR was performed using the NEB Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with cycling conditions of 10 min at 55°C for reverse transcription ...
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bioRxiv - Immunology 2022Quote: ... was digested with BamHI and dephosphorylated using the Quick Calf Intestinal Alkaline Phosphatase kit (M0525L, New England Biolabs). CIP was heat-inactivated and the linearized pGEX-4T1 vector was gel-purified.
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bioRxiv - Genetics 2022Quote: ... PCR-products were gel purified after agarose gel electrophoresis with Monarch DNA Gel Extraction Kit (New England Biolabs) and submitted for sequencing ...
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bioRxiv - Developmental Biology 2022Quote: ... Genomic DNA was collected from each fin clip using the Monarch Genomic DNA Purification kit (New England Biolabs) and amplified using OneTaq 2X Master Mix (NEB ...
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bioRxiv - Genetics 2019Quote: ... Illumina sequencing adapters were added using the NEBNext Library Prep Kit for Illumina (New England Biolabs, Ipswitch, MA) using 1:10 diluted adapters and the optional size-selection step ...
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bioRxiv - Microbiology 2019Quote: Sequencing libraries were prepared from genomic DNA using the NEBNext Ultra DNA Library Prep Kit (New England Biolabs) and sequenced in paired end mode on Illumina HiSeq or MiSeq machines ...
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bioRxiv - Molecular Biology 2019Quote: ... ChIP-seq libraries were generated using NEBNext Ultra II DNA library prep kit following the manufacturer’s protocol (NEB), and sequenced on an Illumina NextSeq500 system using the 75bp high output sequencing kit ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR amplicon libraries were generated using NEBNext Ultra II DNA library prep kit following the manufacturer’s protocol (NEB), and sequenced on an Illumina NextSeq500 system using the 75bp high output sequencing kit ...
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bioRxiv - Developmental Biology 2019Quote: ... First Strand cDNA was synthesized using the Protoscript M-MuLV First-Strand cDNA Synthesis Kit (New England Biolabs). Equal concentrations of RNA (1ug ...
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bioRxiv - Molecular Biology 2019Quote: ... Input sample libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced on an Illumina HiSeq2000 at the Tayside Centre for Genomics Analysis ...
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bioRxiv - Genomics 2019Quote: DNA whole genome libraries were prepared using NEBNext® UltraTM II DNA Library Prep Kit (#E7645L, NEB Inc). DNA was quantified on QubitTM 3.0 flourometer using Qubit High sensitivity dsDNA Assay (#Q32854 ...
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bioRxiv - Microbiology 2019Quote: ... Sequencing libraries were prepared either by NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB) or by Accel-NGS® 1S Plus DNA Library Kit (Swift Biosciences ...
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bioRxiv - Microbiology 2019Quote: The DNA oligo i116 that served as a 3’ adapter was adenylated using 5’ DNA Adenylation Kit (NEB), purified by ethanol precipitation as above and diluted to 10 μM with nuclease-free water.
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bioRxiv - Cancer Biology 2019Quote: ... NGS libraries were prepared using the NEBnext Ultra II DNA library preparation kit for Ilumina (New England Biolabs) according to manufacturer’s recommendations ...