Labshake search
Citations for New England Biolabs :
4901 - 4950 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 2019 [36] with the modification that 3 µg of gDNA per technical replicate were digested using Nucleoside Digestion Mix (NEB M0649S ...
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bioRxiv - Systems Biology 2022Quote: ... 10 μl of eluted DNA were mixed with 40 μl of A-tailing mix (0.25 mM dATP, 125 U/ml Klenow fragment (cat. # NEB M02105S), and Klenow buffer 1.25 X ...
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bioRxiv - Synthetic Biology 2022Quote: ... The reactions were done using 150 ng of each part of the TU and either used the same recipe as level 1 or by using the NEB Golden Gate Assembly Mix (New England Biolabs) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... plasmid pJAM3389 and overlapping primer pair 11/12 were used to amplify a linear plasmid by PCR that was DpnI treated to remove template and ligated using a KLD Enzyme Mix (NEB). For deletion of hvo_1043 ...
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bioRxiv - Microbiology 2021Quote: ... We followed the input DNA mass recommendation of 1.0 µg gDNA and performed DNA repair (NEBNext FFPE DNA Repair Mix, NEB M6630) to maximize read length ...
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bioRxiv - Biochemistry 2020Quote: ... NC-siRNA sense: 5’-AGGUAGUGUAAUCGCCUUGdTdT-3’.35,36 NEBuffer™ 2 and nucleoside digestion mix were obtained from NEB (Ipswich, MA). Adenosine and N6-methyl adenosine (m6A ...
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bioRxiv - Bioengineering 2021Quote: ... the Bxb1 or equivalent integrase/recombinase AttP sites and the cargo sequence were introduced into a minicircle parental plasmid with Gibson cloning using Hifi Assembly mix (NEB) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we added 10 ng of template plasmid to 100 μl of a PCR reaction mix that contains 10 μl of 10×ThermoPol buffer (M0267L, NEB), 2.5 μl of Taq DNA polymerase (M0267L ...
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bioRxiv - Genomics 2022Quote: ... For the GpC methyltransferase treatment, freshly made GpC methyltransferase mix (1x M.GpC buffer, 300 mM sucrose, 64 µM SAM (NEB, #B9003S)) and 200 U M.CviPI (NEB ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Amplifications were performed in an Applied Biosystems 7500 Fast Real-Time PCR System using the Luna Universal Probe Mix (NEB). We evaluated the performance of the qPCR primers and probes using a DNA concentration gradient spanning 0.1-100.0 ng/reaction in single gene reactions as well as in a multiplex reaction ...
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bioRxiv - Genomics 2022Quote: RNA samples from 2 differentiation time courses with 5 time points were used to synthesize full-length barcoded cDNA libraries using the Template Switching RT Enzyme Mix (NEB). Libraries were prepared using Pacific Biosciences protocol for cDNA Sequence Capture Using IDT xGen® Lockdown® probes (https://eu.idtdna.com/site/order/ngs) ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR fragments were assembled into the linearized vector (pRS305; integration vector) (Sikorski and Hieter, 1989) using NEBuilder HiFi DNA Assembly mix (#E2621L, NEB) reaction as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The second strand synthesis reaction mix was assembled as follows (NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module: E6111S, NEB): 20 µl of first strand cDNA ...
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bioRxiv - Cell Biology 2019Quote: ... the M2×24 array was cloned into the pUBC-HaloTag-bActinCDS-bActinUTR-MS2V5 using 20 bp of 5’ and 3’ homology to replace the MS2 cassette using the Hifi builder enzyme mix (NEB). This was later followed by further Gibson assembly of the Halo-bActinCDS-bActinUTR-M2×24 insert into the pLenti-puro backbone using NEB Hifi builder ...
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bioRxiv - Genetics 2019Quote: ... and joined in a single step by overlap extension PCR (1st step: equimolar mix of PCR fragments ZEO, HR and HL, NEB Q5 high-fidelity 2X master mix ...
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bioRxiv - Genetics 2019Quote: ... DNA samples were incubated at 20°C for 4h without rotation in a mix of 10 µl of 10x NEB2 buffer (New England Biolabs), 1 mM of a dNTPs mix (10 µl) ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA fragments were purified from the reaction using a Zymo DNA Clean & Concentrator-5 kit (in-house) and amplified with NEBNext High Fidelity PCR Mix (NEB). Library quality was assessed using a Fragment Analyzer system (Agilent) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Insert NGRP or scFv13R4 was assembled into backbone pDEST-ORS-PelB TMT following NEB builder Hi-fi mix protocol (NEB) to create pDEST-ORS-NGRP/scFv13R4 TMT ...
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bioRxiv - Synthetic Biology 2019Quote: ... Approximately 200−250 pmol of RNA was mixed with 400 pmol of reverse primer and 6 μL of dNTP mix (25 mM each, New England Biolabs) in nuclease-free water at a final volume of 35 μL ...
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bioRxiv - Genomics 2020Quote: ... Double-stranded cDNAs were washed with XP Beads then were handled with NEBNext Ultra II End-prep enzyme mix (New England Biolabs). After an XP Bead-washing ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1ul of cDNA was amplified by PCR with primers that amplify about 200 bp surrounding the sites of interest using OneTaq PCR Mix (NEB). The numbers of cycles were tested to ensure that they fell within the linear phase of amplification ...
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bioRxiv - Microbiology 2020Quote: ... In the meantime, we prepared 150 μl of T4 PNK mix (120 μl H2O, 20 μl 10x T4 PNK buffer (NEB), 1 μl Murine RNase Inhibitor (NEB) ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1ul of cDNA was amplified by PCR with primers that amplify about 200 bp surrounding the sites of interest using OneTaq PCR Mix (NEB). The numbers of cycles were tested to ensure that they fell within the linear phase of amplification ...
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bioRxiv - Microbiology 2020Quote: ... before resuspending the beads in 25 μl RNA ligation mix (9 μl H2O, 3 μl 10x T4 RNA ligase buffer (NEB), 0.3 μl 0.1M ATP ...
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bioRxiv - Plant Biology 2020Quote: ... and an aliquot containing 10 µg protein was treated with lambda protein phosphatase reaction mix following the instructions of the manufacturer (New England Biolabs) for 1 h at 30°C.
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bioRxiv - Genomics 2020Quote: ... Purified fragments were mixed at 1:3 molar ratio and assembled with NEBuilder HiFi DNA Assembly Mater Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... the corresponding set of specific primers (Table S1) and digestion of the template from the reaction mix with DpnI (New England Biolabs), following the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... the coding sequence of Fth1 was PCR-amplified (using ProtoScript II Reaction/Enzyme Mix by New England BioLabs, Ipswich, USA).
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bioRxiv - Genomics 2021Quote: ... End fill-in and A-tailing were performed by addition of Klenow Fragment 3’ --> 5’ exo-(Enzymatics) and dNTP mix (10 mM dATP, 1 mM dCTP, 1 mM dGTP New England Biolabs). After ligation to methylated Illumina TruSeq LT v2 adaptors using T4 DNA Ligase rapid (Enzymatics) ...
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bioRxiv - Biochemistry 2021Quote: ... purified RNA was dissolved in 3 μl RNase H reaction mix (1x RNase H buffer [NEB], 40 pmol oligo(dT)12 ...
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bioRxiv - Developmental Biology 2021Quote: ... Biotin removal from unligated fragments was performed by incubating DNA samples for 4h at 20C in a mix of 5 mL of 10X NEB2 buffer (New England Biolabs), 5 mL of a 1mM dNTPs mix ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1× NEB 1 buffer, 1% triton X-100, and 100 U HpaII enzyme [NEB]) and incubated at 37 °C for 3 hours with constant shaking ...
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bioRxiv - Cell Biology 2020Quote: 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1x NEB 1 buffer, 1% triton X-100, and 100 U HpaII enzyme (NEB)) and incubated at 37°C for 3 hours with constant shaking ...
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bioRxiv - Genomics 2019Quote: ... the fragmented genomic DNA was ligated with 1 µL of 10 µM phosphorylated hairpin oligo mix (1 µL of NEB T4 ligase, 1 µL of 10x NEB T4 Ligase buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5–1 μg of circFL-seq cDNA was repaired and dA-tailed by the NEBNext FFPE DNA Repair Mix (NEB) and NEBNext Ultra II End repair/dA-tailing Module (NEB) ...
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An egg-derived sulfated N-Acetyllactosamine glycan is an antigenic decoy of influenza virus vaccinesbioRxiv - Immunology 2021Quote: ... 25 ug of the 2020 QIV was denatured for 10 minutes at 75°C and treated with the Protein Deglycosylation Mix II (New England Biolabs) for 30 minutes at 25°C and 1 hour at 37°C ...
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bioRxiv - Genomics 2021Quote: ... DNA assembly of the secondary library plasmid pool was performed with 125 ng of PCR amplicon and 825 ng of BsmBI-digested pLentiCRISPRv2 in a total reaction volume of 100 µL with HiFI DNA Assembly Mix (NEB) for 30 min at 50°C ...
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bioRxiv - Microbiology 2021Quote: ... The reaction mix used was the NEB Luna Universal following manufacturer’s directions for cycling conditions (New England Biolabs, Ipswich, MA). All samples were run in duplicate ...
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bioRxiv - Bioengineering 2022Quote: All gene amplifications were performed using polymerase chain reaction (PCR) in a 50 µL mix with Q5 High-Fidelity DNA Polymerase (New England Biolabs) according to the manufactureŕs protocol ...
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bioRxiv - Genomics 2022Quote: ... The “End-prep” was performed with 48 μl of sheared DNA in the reaction mixture using NEBNext Ultra II End Repair/dA-Tailing Module and NEBNext FFPE DNA Repair Mix (New England Biolabs (NEB), cat# E7546 ...
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bioRxiv - Microbiology 2022Quote: ... Boiled tRNA was mixed with 12 μL PEG buffer mix (10 μL 50% PEG8000, 2 μL 10 × buffer B0216S; New England Biolabs). 3 μL of 5’ adenylated linkers (Table S3 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Beads were then dried before adding 50 µL DNaseI mix (3 U of DNaseI in 1X DNAse buffer (NEB, #M0303L) and incubated at 37 °C for 10 min ...
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bioRxiv - Genomics 2022Quote: Add 5 μl of dNTP mix (10 mM each) and 5 μl of DNA polymerase I Klenow fragment (NEB, M0210) to the reaction system ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: DNA encoding single-chain relaxins with an N-terminal hemagglutinin signal sequence and C-terminal 6x His-tag were cloned into the pcDNA-Zeo-tetO vector (37) using PCR and NEBuilder HiFi DNA Assembly Mix (New England Biolabs). Fc fusion single-chain relaxins and the Fc N297Q negative control were cloned into pcDNA-Zeo-tetO with an N-terminal hemagglutinin signal sequence ...
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bioRxiv - Molecular Biology 2022Quote: ... and subsequently cloned via Gibson Assembly in the pRRL-EF1a-XhoI-IRES-BlastR plasmid (gift from G. Superti-Furga) using the NEBuilder HiFi DNA Assembly Mix (New England Biolabs). The CRBN/VHL WT and point mutant plasmids were used for lentivirus production and subsequent transduction in RKO CRBN-/- and VHL-/- clones respectively.
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bioRxiv - Molecular Biology 2022Quote: Purified modified RNA and RNA without any chemical modification were digested with the Nucleoside digestion mix (M0649, New England Biolabs) at 37°C for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... The UbC-Fluc2-SV40 pA cassette was synthesized as DNA gBlocks (Integrated DNA Technologies, IDT) and inserted by Gibson assembly using NEBuilder HiFi assembly mix (New England Biolabs). Unless otherwise noted ...
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bioRxiv - Genomics 2024Quote: ... All subsequent steps were performed on that bead-bound DNA fraction including DNA ends repair with repair mix (10Х ligase NEB buffer ...
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bioRxiv - Bioengineering 2024Quote: ... and mCherry inserts were generated via PCR and cloned into the linearized viral backbone as a single fusion protein using HiFi cloning mix (NEB). H2B-iRFP nuclear marker was (Addgene Plasmid #90237) ...
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bioRxiv - Molecular Biology 2024Quote: ... The biotin labeled nuclei were washed once with 1x NEB ligation buffer and resuspended with ligation mix (100 ul 10x NEB ligation buffer ...