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Citations for New England Biolabs :
5051 - 5100 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... then quantified with the NEBNext® Library Quant Kit for Illumina® using qPCR on a StepOne Plus real-time PCR platform (New England Biolabs® Inc., MA, USA). Following qPCR ...
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bioRxiv - Molecular Biology 2020Quote: ... 13.5 μl digestion reaction product was combined with 1.5 μl second-strand synthesis (SSS) buffer and 1 μl of SSS enzyme mix from the NEBNext mRNA Second Strand Synthesis Module (NEB, Cat #E6111) and incubated at 16 °C for 2.5 h ...
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bioRxiv - Molecular Biology 2020Quote: ... 25 μL of the pooled library was mixed with 3.5 μL NEBNext Ultra II End-Prep Reaction buffer and 1.5 μL Ultra II End-prep Enzyme mix (New England Biolabs, Ipswich, MA, USA), incubated for 10 minutes at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Assembly was performed with 1650 ng of BsmBI-digested pLentiCRISPRv2 and 250 ng of amplicon in a total reaction volume of 100 μL with HiFI DNA Assembly Mix (NEB, Ipswich MA) for 30 min at 50°C ...
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bioRxiv - Biophysics 2019Quote: ... NG-Stop was created using the same inverse PCR product as NG-Scarlet and NG-Cherry via blunt end ligation using NEBs KLD Mix (New England Biolabs; Ipswich, MA). The mNeonGreen gene was obtained from the pmNeonGreen-NT plasmid (Allele Biotehcnology ...
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bioRxiv - Microbiology 2021Quote: ... the DNA products from each ChIP assay were first end repaired with end repair enzyme mix (New England Biolabs, Inc., Cat#M6630), then ligated to NEBNext adaptor included in the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (Cat#E7645L ...
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bioRxiv - Plant Biology 2020Quote: ... 1 ∼ 1.5 μl of DNA extract was added to 20 μl of PCR reaction mix containing standard PCR buffer and 1 unit of Taq DNA polymerase (Cat # = M0273, New England Biolabs, Beverly, MA). For long-range PCR ...
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bioRxiv - Microbiology 2020Quote: ... 50µl of lysed cells were transferred to a tube containing 450µL of digestion mix (1X NEB 1 buffer, 1% triton X-100). This process was repeated 11 times (total 12 tubes with 500 µl) ...
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bioRxiv - Bioengineering 2022Quote: ... pBv2-EF-6X and pBv2-U6-6X (Fig. 1c) were constructed by Golden Gate Assembly (NEB Golden Gate Enzyme Mix (BsaI-HF2), New England BioLabs ...
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bioRxiv - Molecular Biology 2020Quote: ... and 3 μL of factor mix (with RNA polymerase, and transcription/translation factors in 10 mM Mg2+) from the PURExpress® Δ Ribosome Kit (New England Biolabs). The reaction buffer was based on Shimizu et al ...
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bioRxiv - Neuroscience 2021Quote: ... Enzyme restriction with DpnII was performed in 30 µl reaction mix (1 µl DpnII, 3 µl DpnII buffer, 10 µl purified DNA and 16 µl H2O; New England Biolabs, Ipswich, MA) and incubation at 37°C for 2h ...
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bioRxiv - Microbiology 2020Quote: ... polymerases and Gibson assembly mix reagents were used to manipulate DNA fragments and plasmids according to manufacturer instructions (New England Biolabs and Promega).
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bioRxiv - Cell Biology 2021Quote: ... YCplac111-Pah1-PrtA was linearized by inverse PCR and re-ligated using the NEBuilder HiFi DNA assembly mix (New England Biolabs, Ipswitch, Massachusetts) so that the Protein A sequence was replaced by a triple HA sequence ...
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bioRxiv - Molecular Biology 2022Quote: ... deglycosylation treatment (Dglyco) consisted of sample incubation for 3 hours at 37°C with 20 nL of Protein Deglycosylation Mix II (NEB, cat. P6044S) per μL of sample ...
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bioRxiv - Biochemistry 2022Quote: ... A 25 μg aliquot of CP-treated CTDNA was diluted to 100 μL of 1X nucleoside digestion mix reaction buffer (NEB, Ipswich, MA) at pH 5.4 and treated with 2.5 μL NEB nucleoside digestion mix (1 μL or 1 U per 10 μg CTDNA ...
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bioRxiv - Genomics 2022Quote: ... The purified DNA was ligated to Adapter Mix (ONT, SQK-LSK109) in the presence of Ligation Buffer (ONT, SQK-LSK109) and NEBNext Quick T4 Ligase (NEB, cat# E6056) for 30 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... with short homologies for Gibson assembly and cloned into human IgG1 or human IgL2 expression vectors using the NEB Hifi DNA Assembly mix (NEB, Cat#E2621L). Plasmid sequences were verified by Sanger sequencing (Genewiz).
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bioRxiv - Molecular Biology 2022Quote: ... the upstream and downstream probes of DLP pairs was ligated by applying a ligation mix containing 1× SplintR buffer (Cat No. B0375S; NEB, Beijing, China), 2.5 U/μL SplintR Ligase (Cat No ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was incorporated into a digested pFLP2 backbone using KpnI and BamHI restriction sites and HiFi ligation mix (NEB, Ipswich, MA). All restriction enzymes are from NEB (Ipswich ...
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bioRxiv - Molecular Biology 2024Quote: Genomic phage DNA was digested using either a combined treatment of DNaseI and ExoI to create single nucleotides or a commercially available nucleoside digestion mix (NEB, Ipswich, USA).
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bioRxiv - Microbiology 2023Quote: ... Damaged double-stranded DNA in the initial DNA sample was repaired using the PreCR® Repair Mix Kit (New England Biolabs, USA) before library construction ...
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bioRxiv - Immunology 2024Quote: ... cDNA for 3’ RACE was generated using RNA from IFN-β treated A549 cells using the Template Switch RT Enzyme Mix (NEB, #M0466) and anchored primers ...
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bioRxiv - Microbiology 2023Quote: ... Barcoded samples were multiplexed and ligated to sequencing adaptor molecules in Adapter Mix II using the NEBNext Quick T4 DNA ligase (New England Biolabs, United States). The ligation products were washed twice with Short Fragmentation Buffer and eluted with Elution Buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... The assembly reaction is conducted in a 25.0 μL assembly reaction mix composed of 1.0 μL NEBridge® Golden Gate Assembly Kit BsaI-HF® v2 (NEB® E1601), 2.5 μL T4 DNA ligase buffer 10X (NEB® B0202) ...
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bioRxiv - Synthetic Biology 2023Quote: BigBlocks were PCR-amplified with dedicated primer pairs in a 25.0 μL PCR reaction mix composed of 0.25 μL Q5® Hot Start High-Fidelity DNA Polymerase 2 U/μL (NEB® M0493), 5.0 μL Q5® 5X buffer (NEB® B9027) ...
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bioRxiv - Synthetic Biology 2023Quote: ... in a 20.0 μL reaction mix composed of 2.0 μL NEBridge® Golden Gate Assembly Kit BsaI-HF® v2 (NEB® E1601), 2.0 μL T4 DNA ligase buffer 10X (NEB® B0202 ...
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bioRxiv - Microbiology 2023Quote: ... Radiolabeling of 50 pmol of RNA substrate was performed in 20 μl of reaction mix using 10 U of T4 PNK (New England Biolabs, cat. M0201) and 3μL of ?32P-ATP (Perkin Elmer ...
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bioRxiv - Genomics 2023Quote: ... Potential nicks in DNA and DNA ends were repaired in a combined step using NEB Next FFPE DNA Repair Mix and NEB Next Ultra II End repair/dA-tailing Module (NewEngland Biolabs, Ipswich, USA) by tripling the incubation time ...
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bioRxiv - Genomics 2023Quote: ... This involved repairing and 3’ adenylation of 1 µg of HMW genomic DNA with the NEBNext FFPE DNA Repair Mix and the NEBNext® Ultra™ II End Repair/dA-Tailing Modules (New Englang Biolab, NEB). Sequencing adapters were then ligated using the NEBNext Quick Ligation Module (NEB) ...
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bioRxiv - Cell Biology 2023Quote: mNeonGreen-CFTR-WT and mNeonGreen-CFTR-F508del were cloned into pMCB497-pTRE-polyA(-)-pPGK-blastR vector backbone (Leto et al., 2019) using the NEB HiFi Assembly mix (NEB, Ipswitch, MA). CFTR was amplified from pShu-eGFP-CFTR-WT and pShu-eGFP-CFTR-F508del (gift from Jon Hanrahan ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µg of tRNA was heat denatured for 5 min at 95°C and subjected to digestion at 37°C for 16 h using Nucleoside Digestion Mix (New England BioLabs, USA, #M0649S). After digestion ...
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bioRxiv - Developmental Biology 2024Quote: ... 200-300ng of each DNA pool was subjected to repair/dA-tailing (End-Prep) with NEB Ultra II End-Prep Enzyme Mix (New England Biolabs, Ipswich, MA) followed by Native Barcode Ligation as specified by ONT with NB01 assigned to the ER pool ...
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bioRxiv - Molecular Biology 2024Quote: ... The NicE-view labeling mix was based on previously published protocols24–26 and consisted of 1x NEBuffer 2 (NEB, Ipswich USA, #B7002S), 30 µM dGTP (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Carla Green as in (Rosensweig et al., 2018) and the modified using Q5 Site-Directed Mutagenesis (New England Biolabs Inc., Ipswich, MA, cat # E0554S) to create the CRY2 TCGA mutants ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Libraries were prepared using NEBNext FFPE DNA Repair Mix in combination with the NEBNext Ultra II DNA Library Prep kits for Illumina (New England Biolabs, Ipswich, MA, USA) per manufacturer recommendations with custom inline barcodes as described in Moore et al ...
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bioRxiv - Biochemistry 2020Quote: ... The PCR amplification was performed with 10 µl of the reverse transcriptase product in a 50 µl PCR mix containing 1 unit of Phusion High-Fidelity DNA polymerase (New England Biolabs, Évry-Courcouronnes, France), 1X HF Phusion buffer ...
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bioRxiv - Genetics 2021Quote: A 25 μL PCR master mix was set up as follows: 2.5 μL Standard Taq (Mg-free) Reaction Buffer (10x) (B9015 New England BioLabs Inc. Ipswich MA USA), 2.0 μL dNTP mix (10mM total ...
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bioRxiv - Genomics 2021Quote: ... 5 μl of 100 ng/μl cDNA diluted from the previous step was combined with 2 μl block mix and 2 μl nuclease free water (NEB, catalog no. AM9937), then the cDNA-block oligo mix was incubated on a thermocycler under the following condition to allow block oligo mix to bind to 5’ and 3’ end of the cDNA molecule ...
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bioRxiv - Genomics 2021Quote: ... The purified product was quantified using Qubit dsDNA HS assay kit and 50ng of the product was further taken for adaptor ligation using adapter mix II and quick T4 DNA ligase (Cat. No. E6056S, New England Biolabs, Ipswich, Massachusetts, USA) followed by 20 mins incubation at room temperature ...
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bioRxiv - Genomics 2024Quote: ... 4.0 μg of the pool DNA was repaired and end-repaired using NEBNext FFPE DNA Repair Mix (New England BioLabs, Ipswich, MA, USA) and the NEBNext UltraII End Repair/dA-Tailing Module (New England BioLabs ...
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bioRxiv - Immunology 2023Quote: ... 1μg total RNA was used for reverse transcription with the NEB Template Switching Reverse Transcriptase Enzyme Mix (New England Biolabs, Ipswich, MA, USA) according to the recommendations of the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 µL ligation mix (2 µL 10x T4 ligase buffer, NEB, 1 µL T4 RNA ligase 2, truncated, NEB, 1 µL Ribolock inhibitor) was added and incubated (1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... we dispensed 5 μL of a sense oligo and then added 40 μL of phosphorylation reaction mix containing 1 μL of T4 Polynucleotide Kinase (PNK; NEB, cat. no. M0201S), 5 μL of T4 PNK buffer (NEB ...
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bioRxiv - Genomics 2023Quote: ... using the Adapter Mix II from the Native Barcoding Expansion 1–12 kit and NEBNext Quick Ligation Reaction Buffer (New England Biolabs, Ipswich, MA, USA) as well as Quick T4 DNA Ligase (New England Biolabs ...
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Chromosome-level assembly of Cucumis sativus cv. ‘Tokiwa’ as a reference genome of Japanese cucumberbioRxiv - Genomics 2024Quote: ... we put 3 μg of the size-selected DNA for end-repair using NEBNext FFPE DNA Repair Mix (NEW INGLAND BioLabs inc., Massachusetts, USA) and NEBNext Ultra II End Repair/dA-Tailing Module ...
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bioRxiv - Cell Biology 2020Quote: ... nuclei were spun down and resuspended in fill-in mix (biotin-14-dATP [Thermo Fisher Scientific], dCTP, dGTP and dTTP [Thermo Fisher Scientific], Klenow Polymerase [NEB], 1x NEB 2 buffer) for 1.5 h at 37°C with rotation ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was mixed with Reaction Mix 1 (9:1 of 12 uM NEB RT random 6N-S to 12 uM oligo-dT(20), 12uM dNTPs ...
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bioRxiv - Microbiology 2021Quote: ... 500 μl of lysed cells were transferred to a tube containing 4.5 ml of digestion mix (1X NEB 3 buffer, 1% triton X-100) and 100 μl of the lysed cells were transferred to a tube containing 0.9 ml of digestion mix ...
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bioRxiv - Molecular Biology 2024Quote: ... After addition of 200 µl nuclease elution mix (NEB nuclease P1 buffer 1 x, MgCl2 5 mM, 0.5 µl NEB nuclease P1, 0.5 µg benzonase) samples were incubated over night at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... Lysed cells were then diluted 10 time in several tubes containing 450 μl of digestion mix (1X NEB 1 buffer, 1% Triton X-100). 100 units of HpaII were added and the tubes were incubated for 2 hours at 37°C ...