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Citations for New England Biolabs :
4201 - 4250 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... using AgeI and NotI restriction sites and the NEBuilder HiFi DNA Assembly kit (New England Biolabs). The vectors were transformed using Omni-max bacteria (C854003 ...
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bioRxiv - Genomics 2024Quote: ... The libraries were indexed for multiplexing using NEBNext multiplex oligos kit for Illumina (New England Biolabs) and sequenced on a NovaSeq sequencer (Illumina ...
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bioRxiv - Genetics 2024Quote: ... Two days after nucleofection total cell RNA was harvested using Monarch Total RNA Miniprep Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... The obtained amplicons were purified using the Monarch PCR & DNA Cleanup Kit (NEB, Ipswich, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Libraries were generated using the Enzymatic Methyl-seq kit for Illumina (#E7120, New England Biolabs, U.S.A.). After enzymatic shearing for 25 min at 37°C with the NEBNext UltraShear enzyme and buffer ...
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bioRxiv - Genomics 2024Quote: ... Libraries were first prepared using NEBNext Ultra II DNA library prep kit for Illumina (NEB, UK), and the prepared libraries were sequenced on an Illumina iSeq 100 to generate a 2×146bp paired-end reads.
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bioRxiv - Genomics 2024Quote: ... The cultures were pelleted and subjected to plasmid DNA isolation using Monarch Plasmid Miniprep Kit (NEB) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The YqjD variants were generated by using the Q5 site-directed mutagenesis kit (NEB, Frankfurt, Germany) and the following primer pairs ...
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bioRxiv - Biochemistry 2024Quote: ... Isolated truncations were produced by deletion PCR using Q5 site-directed mutagenesis kit (New England Biolabs) and contained an N-terminal His-SUMO tag ...
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bioRxiv - Biochemistry 2024Quote: ... The Q63E mutation was introduced using the Q5-Site Directed Mutagenesis Kit (New England BioLabs Inc). Human Avi-tagged Gβ1 and Gγ2 were subcloned into a pCI-neo vector (gift from A.V ...
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bioRxiv - Biochemistry 2024Quote: In vitro transcription was performed with the HiScribe T7 Kit (New England Biolabs, cat. no. E2040S), according to the manufacturer’s instructions using DNA templates containing a T7 promoter upstream of the template sequence ...
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bioRxiv - Bioengineering 2024Quote: ... IVT reactions used the HiScribe® T7 High Yield RNA Synthesis Kit (New England Biolabs, USA) following the manufacturer’s instructions and 3′-O-Me-m7G(5′)ppp(5′)G RNA cap (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: RNA was harvested from cell pellets using the Monarch Total RNA Miniprep Kit (New England Biolabs). cDNA was produced using the Invitrogen RNA-to-cDNA kit ...
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bioRxiv - Evolutionary Biology 2024Quote: ... prepared into Illumina sequencing libraries using either the NEB Ultra II FS kit (NEB, Ipswich, MA) or the TruSeq PCR-free DNA kit (Illumina ...
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bioRxiv - Developmental Biology 2024Quote: RNA extraction from hiPSCs/hESCs was conducted using the Monarch Total RNA Miniprep Kit (NEB T2010S) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The mRNA was purified from human PAECs using Magnic mRNA isolation kit (New England Biolabs, S1550S). In order to obtain the required quantity of RNA ...
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bioRxiv - Developmental Biology 2024Quote: ... The stop codon was then removed using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs # E0554 ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA was extracted from 5×106 cells using a Monarch Genomic DNA extraction kit (NEB). 2 ng of genomic DNA was incubated at 55°C for 15 minutes with 0.4 ul TDE1 (Illumina ...
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bioRxiv - Biophysics 2024Quote: ... separated by agarose gel electrophoresis and purified with the Monarch® DNA Gel Extraction Kit (NEB). The fragments were ligated by sequence-and ligation-independent cloning (SLIC)33 using T4 DNA Polymerase (NEB) ...
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bioRxiv - Cell Biology 2024Quote: ... the DNA product was transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). The resulting sgRNA was purified using the RNeasy Mini kit (74104 ...
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bioRxiv - Cell Biology 2024Quote: ... The cloning procedure was performed using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). The mutations that resulted in the indicated amino acid substitutions were introduced via PCR-mediated site-directed mutagenesis ...
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bioRxiv - Bioengineering 2024Quote: ... 1 µL of NEBridge Golden Gate Assembly Kit (BsmBI-v2) reaction mix (New England Biolabs E1602), nuclease free water up to 10 µL ...
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bioRxiv - Biochemistry 2024Quote: ... Ub1-I44A was generated by introducing point mutations using the Q5-site-directed mutagenesis kit (NEB). Protein was expressed in Rosetta E ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting material was reverse transcribed (M-MLV RT) and purified (NEB Monarch DNA Cleanup Kit). Two rounds of PCR were used to tag each reaction with sequencing barcodes (Illumina ...
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bioRxiv - Biochemistry 2024Quote: ... RNA were extracted using a Mornarch® RNA Cleanup Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacture’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Single amino acid substitutions were introduced using NEB’s site-directed mutagenesis kit (New England Biolabs, E0552S). Primers used for cloning and site-directed mutagenesis are listed in Table S2 ...
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bioRxiv - Cell Biology 2024Quote: ... Sequencing libraries were generated using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645L) and NEBNext Multiplex Oligos (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... cleaned up A-tailed DNA went through Adaptor ligation reaction using Quick Ligation Kit (NEB, M2200L) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Then the RNA was purified by using the Monarch RNA cleanup kit (NEB, cat. no. T2050L) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared with the NEBNext Ultra II RNALibrary prep kit for Illumina (NEB, Cat.No. E7770S).
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bioRxiv - Cancer Biology 2024Quote: ... mRNA expression was determined using SYBR green Luna One Step RT-qPCR Kit (New England BioLabs) on a Bio-Rad C1000 Touch thermocycler ...
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bioRxiv - Genetics 2024Quote: ... or ran on an electrophoresis gel and purified using the Monarch DNA Gel Extraction Kit (NEB) Cat#T1020L ...
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bioRxiv - Genetics 2024Quote: ... and sequencing libraries generated using the NEBNext Ultra II directional RNA library preparation kit (NEB #E7760) with 8 cycles of PCR ...
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bioRxiv - Genetics 2024Quote: Genomic DNA was isolated from cell pellets using the Monarch Genomic DNA Purification Kit (NEB, #T3010L) following the manufacturer’s protocol for cultured cells ...
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bioRxiv - Genomics 2024Quote: NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs; Cat. No. E7805) was used according to manufacturer’s instructions with some modifications ...
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bioRxiv - Genetics 2024Quote: ... Library preparation was carried out using the NEBNext UltraExpress RNA Library Prep Kit (New England Biolabs). mRNA was fragmented and copied into first strand complementary DNA (cDNA ...
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bioRxiv - Cell Biology 2024Quote: ... CTDNEP1 KO and NEP1R1 KO cell lines using Monarch® Total RNA Miniprep Kit (NEB T2010S). RT-qPCR was performed using the Luna Universal qPCR Master Mix (NEB M3003L ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Library preparation was performed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB), following the manufacturer’s recommendations and sequenced on Illumina Hiseq platform and 150 bp paired-end reads were generated.
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bioRxiv - Microbiology 2020Quote: ... and ftsH target sequences was carried out in vitro using a Q5 mutagenesis kit (New England BioLabs) with specified primers (S1 Table) ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was collected at 9 h post-IFN treatment using Monarch Total RNA Miniprep Kits (NEB). RNA was prepped for RNA sequencing submission using the NEBNext Poly(A ...
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bioRxiv - Synthetic Biology 2021Quote: ... PCR reactions were purified with Monarch DNA Cleanup Kit columns (T1030; New England Biolabs; Ipswich, MA, USA) per the manufacturer’s protocol and eluted in 20 µL nuclease-free water.
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bioRxiv - Genomics 2021Quote: ... 1μl RT Adapter (110nM; ONT Kit) and 1.5μl T4 DNA Ligase (2M U/ml New England BioLabs). The ligation reaction was carried out for 10min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... ΔN1/2/3 and ΔDAD mutants were generated by Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) with HOXB13 WT or NCOR1 WT as a template ...
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bioRxiv - Biophysics 2020Quote: ... FR-Q and FR-MQ were made using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with pBad-FR and pBad-FR-M ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was transcribed to cDNA using NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB E7530). RNA sequencing was performed using single-end 75 bp reads on an Illumina NextSeq500 ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) with size selection for ~200 bp insert DNA ...
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bioRxiv - Cell Biology 2020Quote: ... Site directed mutagenesis to generate GATA4 V266M was performed with the Q5 Site-Directed Mutagenesis Kit (NEB) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Sequencing library preparation was performed using the NEBNext Ultra DNA library prep kit for Illumina (NEB, E7370S), following manufacturer instructions ...