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Citations for New England Biolabs :
4101 - 4150 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
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bioRxiv - Developmental Biology 2024Quote: ... plasmid templates were linearized with NotI and purified using Monarch PCR purification kits (New England Biolabs). The purified templates were transcribed using mMESSAGE mMACHINE Sp6 (Thermo-Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... and reversely transcribed into cDNA using the ProtoScript II NEB cDNA synthesis kit (New England Biolabs).
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bioRxiv - Genomics 2024Quote: ... Sequencing adapters were ligated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) while DNA was bound to the beads ...
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bioRxiv - Genomics 2024Quote: ... The recovered DNA was subjected to library construction using NEBNext Ultra DNA library prep kit (NEB). The sequencing libraries were amplified by PCR using the NEBNext Ultra II Q5 Master Mix (NEB ...
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bioRxiv - Microbiology 2024Quote: ... DNA was subsequently purified with a Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Val206 of Envy was mutagenized to Lys with the Q5 Site-Directed Mutagenesis Kit (NEB #E0554S) using primers listed in Table S2 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... After the reaction we purified the PCR product with Monarch PCR and DNA Cleanup Kit (NEB).
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of purified DNA template was used in the Hi-Scribe T7 transcription kit (NEB) at 37°C overnight (∼16 hr.) ...
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bioRxiv - Genomics 2024Quote: ... and final library amplification were performed using the NEBNext Enzymatic Methyl-seq Kit (New England BioLabs) for WGEM-seq libraries ...
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bioRxiv - Cancer Biology 2024Quote: ... was used for library preparation of total RNAs and Next small RNA Kit (New England Biolabs) for library preparation of microRNAs according to manufacturerś instructions ...
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Evolutionary Adaptation of an HP1-protein Chromodomain Integrates Chromatin and DNA Sequence SignalsbioRxiv - Genetics 2024Quote: ... Barcoded libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to manufacturer’s instructions and sequenced on a NovaSeqSP instrument (Illumina).
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bioRxiv - Cancer Biology 2024Quote: ... All single-cell libraries were prepared using the NEBNext Ultra II FS kit (New England Biolabs) following the manufacturer’s instructions but using one-fourth volumes ...
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bioRxiv - Genetics 2024Quote: ... Up to 1µg was the used for reverse transcription using the ProtoScript Reverse Transcription kit (NEB). qPCR was done using iTaq universal SYBR Green Supermix (BioRad ...
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bioRxiv - Genetics 2024Quote: ... A NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) was used for library preparation ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative RT-PCR was conducted with the Luna Universal One-Step RT-qPCR Kit (NEB, E3005) on a Bio-Rad CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... The R1R DNA adapter was adenylated by using a 5’ DNA Adenylation kit (New England Biolabs) and then ligated to the 3’ end of the cDNA by using Thermostable 5’ App DNA/RNA Ligase (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB, E7645L) and single-end (1 × 75 bp ...
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bioRxiv - Cancer Biology 2024Quote: ... the DNA fragments were purified from gel electrophoresis using the Monarch DNA gel extraction kit (NEB), fragments and destination plasmid were digested using the respective restriction enzymes (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting PCR product was cloned into the pCDF4 vector using the Gibson Assembly kit (NEB) and the resulting plasmid was then used to generate ATG4-gRNA transgenic flies via injection into the y1 ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesised according to the protocols of the LunaScript RT Supermix Kit (New England Biolabs) and for qPCR amplification ...
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bioRxiv - Immunology 2024Quote: ... and subsequently replaced with the tailed rpoS DNA fragment using the HiFi DNA assembly kit (NEB). Following the assembly reaction ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were prepared with the NEBNext Ultra RNA library prep kit for Illumina (NEB, 7530L) according to the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were prepared with the NEBNext Ultra RNA library prep kit for Illumina (NEB, 7530L) according to the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2024Quote: First strand cDNA was synthesized from DNase-treated RNA with LunaScript® RT SuperMix Kit (NEB). Prepared cDNA was amplified in Real Time utilizing the StepOnePlus real-time PCR system (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... using the manufacturer’s protocol except that a Monarch® RNA Cleanup Kit (10 µg; NEB# T2030) was used to purify the rRNA-depleted RNA instead of magnetic beads ...
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Novel RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteriabioRxiv - Molecular Biology 2024Quote: ... In vitro translation was performed with the PURExpress in vitro protein synthesis kit (New England Biolabs). The 10 µL PURExpress reactions with or without 2 µM YebC variants were preincubated at 37°C for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... RNAs were in vitro transcribed using the HiScribe T7 High Yield Kit (New England Biolabs SE20405), substituting 25% of the UTP concentration with Bio-16-UTP (Invitrogen AM8452) ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA was isolated using the Monarch Total RNA MiniPrep Kit (Cat #T2010S, New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and then purified via the Monarch Total RNA Miniprep Kit (New England Biolabs, Ipswich, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared by the FCGZ using the NEB Ultra II DNA Library Prep Kit (NEB) for Illumina according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Linearized pENTRY-miniCR vectors and both flanks were fused using the Quick Ligation™ Kit (NEB). Correct sequences of miniCRs and flanks were verified by Sanger sequencing and consequently transferred from pENTRY vectors into destination vector pIZ-GW36,108 using the Gateway™ LR Clonase™ II Enzyme mix (Invitrogen™).
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bioRxiv - Microbiology 2024Quote: ... The libraries were prepared using the NEBNext Ultra II DNA Library Preparation Kit (New England Biolabs), followed by hybridization with 120-mer RNA baits (SureSelect Target Enrichment System ...
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bioRxiv - Microbiology 2024Quote: ... Library preparation was performed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) per kit directions for intact RNA (15 minute fragmentation ...
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bioRxiv - Microbiology 2024Quote: ... RNA library construction was carried out using Ultra II RNA Library Prep Kit for IIIumina (NEB). RNA integrity and library size were assessed with the Agilent tape station (RIN > 6) ...
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bioRxiv - Microbiology 2024Quote: ... RNA-seq libraries were constructed using the NEB Ultra II RNA custom kit (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids containing invR mutants were constructed with Q5 Site-Directed Mutagenesis Kit (New England BioLabs, E0554S).
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) following product guidelines with an RNA fragmentation time of 10 min ...
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bioRxiv - Immunology 2024Quote: ... The immunoprecipitated chromatin samples were used for library preparation with NEBNext Ultra II Library kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Extracted RNA was amplified using Luna® Universal One-Step RT-qPCR Kit (NEB, Ipswich, MA) according to the manufacturer’s directions on a QuantStudio3 (Applied Biosystems ...
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bioRxiv - Genomics 2024Quote: ... dA-tailing and sequencing adapter ligation with the NEB Next Ultra II kit (New England Biolabs) according to manufacturer’s instructions with no modifications ...
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bioRxiv - Genomics 2024Quote: ... then purified DNA was fragmented and adaptor-ligated with NEBNext Ultra II FS kit (NEB #E7805) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... the RNA in the reaction was purified using the Monarch RNA Cleanup Kit (50 µg, NEB).
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bioRxiv - Microbiology 2024Quote: ... we modified the plasmid pSag1-Cas9-U6-sgUPRT [45] using Q5 Site-Directed Mutagenesis Kit (NEB) to replace the UPRT guide RNA sequence within the plasmid to a guide RNA sequence of the target gene ...
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bioRxiv - Developmental Biology 2024Quote: ... The five PCR products were GIBSON assembled using the NEBBuilder HiFi DNA assembly kit (NEB, E2621). The vector was then assembled to incorporate the AAV2 ITR sequences from U1a Nme2Cas9 rAAV (Addgene ...
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bioRxiv - Microbiology 2024Quote: ... 1 µg of total RNA was processed using NEBNext® rRNA Depletion Kit (Bacteria) (NEB, E7850). Sequencing libraries for RNA-Seq were constructed using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB ...
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bioRxiv - Microbiology 2024Quote: ... The product was purified using the Monarch DNA & PCR Cleanup kit (New England Biolabs, Ipswich, MA), following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... The product was purified using the Monarch DNA & PCR Cleanup kit (New England Biolabs, Ipswich, MA), following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2024Quote: ... for AST or the HiScribe SP6 Quick High Yield RNA Synthesis Kit (New England Biolabs, #E2070S) for sense env transcripts ...
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bioRxiv - Microbiology 2024Quote: ... RNA was transcribed using HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, #E2050S) for AST or the HiScribe SP6 Quick High Yield RNA Synthesis Kit (New England Biolabs ...