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Citations for New England Biolabs :
4151 - 4200 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Sample libraries were quantified using the NEBNext Library Quant Kit for Illumina (New England Biolabs, UK). Sequencing was performed on an Illumina MiSeq platform (Illumina Inc. ...
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bioRxiv - Systems Biology 2024Quote: ... and prepared for sequencing using the NEBNext UltraII RNA Library Prep Kit for Illumina (NEB #E7770).
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bioRxiv - Synthetic Biology 2024Quote: ... and RNA was extracted using the Monarch Total RNA Miniprep Kit (New England BioLabs Inc., T2010S) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and mRNA libraries were prepared with the NEBNext Ultra RNA Library Prep Kit (New England Biolabs) and barcoded using NEBNext Multiplex Oligos ...
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bioRxiv - Genomics 2024Quote: ... Libraries were constructed using the DNA Ultra FSII Library Prep Kit (New England Biolabs; Cat# E7430). Cells were sequenced 150 base-pairs paired end on an Illumina HiSeq 4000 (Fulgent ...
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bioRxiv - Genomics 2024Quote: ... Library preparation was performed using NEBNext Ultra DNA library prep kit for Illumina (New England BioLabs) and ligated with methylated adapters for Illumina sequencing at a dilution of 1:10 (New England BioLabs) ...
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bioRxiv - Immunology 2024Quote: ... Sequencing libraries were generated using NEB Next Ultra Directional RNA Library Prep Kit for Illumina (NEB) in accordance with the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... The amplification products were purified using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs), and the final constructs were obtained using HiFi assembly (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... Samples then underwent RNA purification using the Monarch RNA clean-up kit (NEB product number T2030S), modified to increase the washing step to a total of five times to ensure removal of free DTB-GTP ...
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bioRxiv - Immunology 2024Quote: Total RNA isolation of samples was performed using Monarch Total RNA Miniprep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was then purified from the quenched reaction mixtures using Monarch® RNA Cleanup Kit (NEB) according to manufacturer protocols and subsequently quantified by NanoDrop.
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bioRxiv - Cell Biology 2024Quote: ... Eluted DNA was enriched by PCR using Phusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Library preparation was performed using the NEBNext Ultra RNA libray Prep Kit for Illumina (NEB, USA). RNA sequencing was performed using the Illumina NovaSeq platform ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA synthesis was performed using the Luna script RT Supermix kit (New England Biolabs, Cat#M3010L). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Cell Biology 2024Quote: ... cleaned up A-tailed DNA went through Adaptor ligation reaction using Quick Ligation Kit (NEB, M2200L) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were produced with a NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced on an Illumina NovaSeq 6000 platform using a 2 × 150 bp run protocol ...
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bioRxiv - Immunology 2024Quote: ... In vitro transcription was then done with the HiScribe T7 High Yield RNA Synthesis Kit (NEB), template DNA was removed with DNase I (NEB) ...
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bioRxiv - Microbiology 2024Quote: A NEBNext® Ultra™ II DNA Library Prep Kit (New England Biolabs, cat. no. E7645S) protocol for Illumina was used to prepare DNA libraries for sequencing ...
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bioRxiv - Bioengineering 2024Quote: ... and cDNA libraries were generated by RT-PCR using the ProtoScript II cDNA Synthesis Kit (BioLabs) according to the manufacturers’ protocols ...
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bioRxiv - Developmental Biology 2024Quote: ... dsDNA was converted to RNA with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, E2040S) and purified using Monarch® RNA Cleanup Kit (NEB ...
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High-resolution chromosome-level genome provides molecular insights into adaptive evolution in crabsbioRxiv - Genomics 2024Quote: ... Sequencing libraries were constructed using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA), following the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: ... and libraries for Illumina sequencing were performed using the NEBNext Ultra II RNA kit (NEB, #E7770L). Quantification of the library was performed using dsDNA HS Assay Kit and Qubit 2.0 (Molecular Probes ...
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bioRxiv - Zoology 2024Quote: ... RT-qPCR was performed using the Luna One-step RT-qPCR kit (New England Biolabs, Germany) on a 384 well-plate with 3 technical replicates per biological replicate in CFX384 Touch Real-Time PCR (Bio-Rad ...
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bioRxiv - Synthetic Biology 2024Quote: ... A NEB Monarch® Plasmid Miniprep Kit was used for RT-DNA extraction (New England Biolabs). Following miniprep ...
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bioRxiv - Microbiology 2024Quote: ... UDI-barcoded RNAseq libraries were generated with the NEBNext Ultra II RNA Library Prep Kit (NEB). Each library was quantified with a Qubit (dsDNA HS kit ...
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bioRxiv - Immunology 2024Quote: ... Further modifications were performed by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (NEB). All modifications to the 2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO plasmid (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of total RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs) according to the manufacturer’s instructions and 1 µl cDNA was used as a template for a 10 µl qPCR reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, E7645) following manufacture’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries were prepared using a NEBNext Ultra Directional RNA prep kit (New England Biolabs, Ipswich, MA) using the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries were quantified using the NEBNext® Library Quant Kit (New England Biolabs, Cat. No. E7630) on an Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System ...
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bioRxiv - Neuroscience 2024Quote: ... The concentration of libraries was determined using NEBNext library Quant kit for Illumina (NEB, Cat# E7630). Libraries were then pooled and sequenced by Macrogen on Ilumina Hiseq X-ten platform ...
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bioRxiv - Cancer Biology 2024Quote: ... and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB, E7760S, USA) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... The amplicons were cleaned up using a Monarch® PCR & DNA cleanup kit (catalog #: T1030S, NEB). The 3’UTR fragments were then cloned into restriction sites (NheI and BamHI ...
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bioRxiv - Molecular Biology 2024Quote: ... Multiple mutation sites were achieved using the Q5® Site-Directed Mutagenesis kit (NEB, catalog #: E0554S) according to their protocols ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were generated with the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB). Library concentration and purity was verified using the Qubit dsDNA HS kit (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep kit (New England Biolabs). RNA quality was assessed using High Sensitivity RNA Screentape and an Agilent 4200 tapestation ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using the NEBNext UltraExpress RNA Library Prep Kit (New England Biolabs, Ipswich, MA), following the manufacturer’s protocol using 150 ng RNA per sample ...
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bioRxiv - Microbiology 2024Quote: ... bacterial rRNA-depleted samples were prepared by using the NEBNext rRNA Depletion kit (NEB, NA, USA). After rRNA depletion ...
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bioRxiv - Neuroscience 2024Quote: ... Reverse: 5’-GCTGCCAGTCCAATAGAGGG-3’) were used with the Luna Universal One-Step RT-qPCR Kit (NEB) with SYBR Green detection on the CFX96 (Bio-Rad ...
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Spatial 3D genome organization controls the activity of bivalent chromatin during human neurogenesisbioRxiv - Neuroscience 2024Quote: ... Samples were depleted of ribosomal RNA (rRNA) using the NEBNext rRNA Depletion kit v2 (NEB, E7400L). Libraries for sequencing were prepared using the NEBNext Ultra II directional library kit (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... The sgRNAs were synthetised using the EnGen® sgRNA Synthesis Kit (New England BioLabs, USA, #E3322V), according to their standardized protocol ...
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bioRxiv - Plant Biology 2024Quote: Minipreps were performed using Qiagen kits and DNA in-gel purification using MonarchR (New England Biolabs). For cloning ...
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bioRxiv - Cancer Biology 2024Quote: ... In vitro transcription of each gRNA was performed using the EnGen sgRNA Synthesis Kit (NEB #E3322), according to manufacturers directions ...
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bioRxiv - Neuroscience 2024Quote: ... the individual sgRNAs were multiplexed in the same plasmid using NEBridge Golden Gate Assembly kit (NEB) with custom designed primers and cloned into the lentiviral pVLX-EF1a-GFP backbone ...
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bioRxiv - Synthetic Biology 2024Quote: ... we used column purification kits to isolate and purify DNA (New England Biolabs, catalog number: T1110). DNA was eluted with ddH2O equilibrated to 56 °C.
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bioRxiv - Cancer Biology 2024Quote: RNA was harvested from cell pellets using the Monarch Total RNA Miniprep Kit (New England Biolabs). Library preparation and sequencing was performed at Washington University School of Medicine GTAC.
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bioRxiv - Biophysics 2024Quote: ... If the PCR product was pure then the sample was purified (NEB DNA Clean-up kit). 100 ng of purified DNA was used as a template for in vitro transcription (NEB E2040S ...
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bioRxiv - Genomics 2024Quote: ... ChIP-seq libraries were prepared by using the NEBNext Ultra II DNA prep kit (NEB E7645) following the manufacturer’s instruction and sequenced on a NextSeq 500 sequencer (Illumina).
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bioRxiv - Biochemistry 2024Quote: ... The amidases were cloned into MCS1 of pCDFDuet-1 using Gibson assembly kit (New England Biolabs) after linearization of the vector with NcoI and HindIII (Fermentas) ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription and PCR amplification were performed with the Luna One-Step RT-qPCR kit (NEB). Real time PCR amplification was performed on a BioRad CFX96 Real-Time PCR detection system ...