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Citations for New England Biolabs :
4051 - 4100 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and library construction was executed using NEBNext Ultra RNA Library Prep kit (New England Biolabs, USA), and sequenced on a NovaSeq6000 ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by library preparation using NEBNext® Ultra™ II RNA Library Preparation Kit (NEB, E7770S). Libraries were paired-end sequenced with read lengths of 150 bp on Illumina HiSeq X Ten or Nova-seq instruments ...
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bioRxiv - Molecular Biology 2022Quote: ... The final pool was quantified using NEBNext Library Quant Kit for Illumina (New England Biolabs, E7630L) and paired-end sequenced on an Illumina MiSeq.
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bioRxiv - Plant Biology 2022Quote: ... Sequencing libraries were prepared with a NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) and sequenced on an Illumina HiSeqX10 platform using a 150-bp paired-end sequencing strategy at Novogene Co. ...
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bioRxiv - Neuroscience 2022Quote: ... RNAs were used to synthesize cDNAs using the ProtoScript cDNA synthesis kit (New England Biolabs, USA) and subjected to RT-PCR analyses to determine STAU1-RNA interactions ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were generated using NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, #E7645L) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Genetic substitutions were introduced via site-directed mutagenesis using the Q5 site-directed mutagenesis kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... O-GlcNAc site were mutated into alanine with Q5® Site-Directed Mutagenesis Kit (NEB#E0554). Lentivirus were packed as previously described (Huang et al. ...
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bioRxiv - Plant Biology 2023Quote: ... Sequencing libraries were generated using NEB Next Ultra RNA Library Prep Kit for Illumina (NEB, USA) as reported previously (Jiang et al. ...
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bioRxiv - Systems Biology 2023Quote: ... we pelleted each liquid culture by centrifugation and used the Monarch Plasmid Miniprep Kit (NEB #T1010L) to isolate plasmid according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... The libraries were indexed for multiplexing using NEBNext multiplex oligos kit for Illumina (New England Biolabs). A total of 54 libraries were generated ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA libraries were prepared using the NEBNext Multiplex Small RNA Library Prep kit for Illumina (NEB) in accordance with the manufacturers’ instructions.
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bioRxiv - Neuroscience 2023Quote: ... An HpaI site within W3SL was destroyed using the Q5® Site-Directed Mutagenesis Kit (NEB): pAAV-U6-TATAlox-CMV-EGFP-TATAlox-W3SL ...
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bioRxiv - Cancer Biology 2023Quote: ... [32P]-labelled AdML pre-mRNA was synthesized in vitro using the T7 HiScribe Transcription Kit (NEB) using [α-32P] UTP (Perkin Elmer ...
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bioRxiv - Genetics 2023Quote: ... Illumina libraries were generated using the NEBNext Ultra II Dna Library Prep Kit for Illumina (NEB), as described previously49 ...
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bioRxiv - Genetics 2023Quote: ... CBX1 mutations were introduced using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs Inc., E0554S) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... TrAEL-seq adaptor 2 was added using a modified NEBNext Ultra II DNA kit (NEB E7645S): 3.5 μl NEBNext Ultra II End Prep buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Two micrograms of total RNA was circularized by a T4 RNA Ligase 1 Kit (M0204. NEB) and then purified by TRIzol reagent followed by isopropanol precipitation ...
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bioRxiv - Cancer Biology 2023Quote: ... Genomic deoxyribonucleic acid (gDNA) was extracted using Monarch Genomic DNA Purification Kit (New England BioLabs, T3010S) following procedures for Tissue gDNA isolation ...
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bioRxiv - Genomics 2022Quote: ... RNA was harvested 72 hours after transfection using the Monarch Total RNA Miniprep Kit (NEB #T2010). mScarlet and HPRT (housekeeping gene for normalization ...
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bioRxiv - Genomics 2022Quote: ... NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® (E7760S, New England Biolabs) and NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (E7645S ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was completed using NEBNext Ultra II Directional RNA Library Prep kits (New England Biolabs), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, #E7645S) with NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 1 (NEB ...
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bioRxiv - Genomics 2023Quote: ... and the pooled library was further quantified using a NEBNext Library Quant Kit (New England Biolabs). The RNA-seq libraries were subjected to two rounds of 75bp paired end sequencing on a NextSeq 550 platform using a 150-cycle kit (Illumina).
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bioRxiv - Genomics 2023Quote: ... and the pooled library was further quantified using a NEBNext Library Quant Kit (New England Biolabs). The CUT&Tag libraries were subjected to 50bp paired end sequencing on a NextSeq 2000 platform using a P2 100-cycle kit (Illumina) ...
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bioRxiv - Cell Biology 2023Quote: ... diluted 1:100 and used for the ligation using the Quick Ligation Kit (New England Biolabs). Specific base pair substitutions were introduced with site directed mutagenesis by polymerase chain reaction (PCR) ...
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bioRxiv - Genomics 2023Quote: Sequencing libraries were prepared using NEBNext Ultra II DNA library prep Kit for Illumina (NEB #E7645S) according to the manufacturer’s instructions for MNase ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) to produce stranded cDNA libraries ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA library preparation using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB), and sequencing was performed at the Yale Stem Cell Center Genomics Core facility ...
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bioRxiv - Cell Biology 2023Quote: Amplified ssDNA library was purified with the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB). For this ...
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bioRxiv - Cell Biology 2023Quote: In vitro transcription was done using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB), following the manufacturer’s instructions for short transcripts ...
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bioRxiv - Systems Biology 2023Quote: ... Libraries were prepared with the NEBNext Ultra II DNA library preparation kit (New England Biolabs, E7645L), quantified by Qubit fluorimetry ...
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bioRxiv - Genomics 2023Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Preparation Kit (New England Biolabs) and sequenced on Illumina NextSeq500 (75 bp in single-end mode ...
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bioRxiv - Genomics 2023Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Preparation Kit (New England Biolabs) and sequenced on Illumina NextSeq500 platform (75 bp in single-end mode).
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bioRxiv - Neuroscience 2023Quote: ... and HEK-293 cells were extracted using Monarch Total RNA Miniprep Kit (New England BioLabs, T2010S). To prepare RNA-Seq libraries ...
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bioRxiv - Systems Biology 2023Quote: ... RNA was synthesised using T7 RNA polymerase (HiScribe T7 High Yield RNA Synthesis Kit, NEB, E2040S). Then RNA was reverse- transcribed using a recombinant Moloney leukemia virus reverse transcriptase (GeneAce cDNA Synthesis Kit ...
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bioRxiv - Biochemistry 2023Quote: ... RT-PCR reactions were performed using the OneTaq One-Step RT-PCR Kit (New England BioLabs) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... H259A and H353A were cloned using site directed mutagenesis (Q5® Site-Directed Mutagenesis Kit, NEB) using primers TCATACAGCAGCGGTCCAGTTAC (forward ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and cloned into pUC19 (digested with EcoRI plus HindIII) using the NEBuilder kit (New England Biolabs). The cloning products were then transformed into E ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was performed with the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) according to manufacturer’s instruction on an iQ5 Real-Time PCR System (Bio-Rad) ...
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bioRxiv - Genetics 2023Quote: ... The library was generated with NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB; 7770), and sequencing was done using the NovaSeq 6000 S4 platform with PE150 ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were constructed using the NEBNext II directional RNA library kit for Illumina sequencing (NEB) and sequenced on a NextSeq2000 platform at the Epitranscriptomics and RNA-sequencing facility ...
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bioRxiv - Neuroscience 2023Quote: ... single histidine point mutations were generated using the Q5 Site-Directed Mutagenesis kit (New England Biolabs) with DNA oligonucleotides incorporating the mutation ...
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bioRxiv - Cancer Biology 2023Quote: ... Sik3 and Lkb1 were cloned into pXPR-RFP-Blast using the Gibson Assembly Kit (E2611L, NEB). The target sequences are as follows ...
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bioRxiv - Cell Biology 2023Quote: ... total RNA isolation was performed by using the Monarch Total RNA Miniprep Kit (New England BioLabs) and RNA concentration was detected in a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... 2021.9 All three fragments were cloned via Gibson assembly using a HiFi DNA assembly kit (NEB) into a vector backbone containing PiggyBac transposition sites.35 The Cer1:H2B-Venus reporter construct was co-transfected with CAG-pBASE35 using Lipofectamine 2000 (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was derived from total RNA using ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560S). Numerous primers were designed against cryptic exon targets and screened to identify primer pairs that minimized background bands.
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bioRxiv - Molecular Biology 2023Quote: ... The samples were prepared using a Luna Universal One-Step RT-qPCR Kit (New England Biolabs). Quantitative RT-PCR was performed with an Mx3000P qPCR system (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... va mtSSB ΔMTS by PCR amplification and ligation (Q5 Site-Directed Mutagenesis Kit, NEB, Cat #E0554). The plasmid insert was sequence-verified and then transformed into E ...
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bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...