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Citations for New England Biolabs :
4001 - 4045 of 4045 citations for 7 11 Dimethyldodeca 4 6 10 trien 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... at a final volume of 20 μL with 10 μL of Luna® Universal qPCR Master Mix (New England Biolabs, Frankfurt, Germany). Each primer was added at a final concentration of 300 nM ...
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bioRxiv - Developmental Biology 2023Quote: ... The PCR product was injected into N2 worms at 10-20 ng/μl with 50 ng/μl pRF4 and 50 ng/μl DNA ladder (NEB, Ipswich, MA). The pRF4 plasmid produces a dominant roller phenotype ...
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bioRxiv - Genetics 2023Quote: ... PCR was then performed in a mix of 10 µM Nextera i7 and i5 primers and NEBNext Q5 High-Fidelity PCR Master Mix (New England Biolabs, MA, USA) according to the following protocol ...
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bioRxiv - Microbiology 2024Quote: ... The master mix for the entire plate included 10 µl of OneTaq Quick-Load 2X Master Mix with standard buffer (New England Biolabs, MA, USA), 0.5 µl of each primer (10 µM) ...
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bioRxiv - Systems Biology 2024Quote: ... The V4-V5 16S variable regions were first amplified in 10 µL reactions in triplicate using the NEB Q5 Hot Start HiFi 2X master mix (New England BioLabs, Ipswich, MA) and 515F and 926R primers as described 59 ...
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Pre-implantation genome-wide methylation enables environmental adaptation in a social meso-carnivorebioRxiv - Genomics 2024Quote: Samples of ∼200 ng genomic DNA were digested with 20 units of MspI (C↓CGG) and 10 units of AluI (AG↓CT) (New England Biolabs, United Kingdom) at 37°C overnight (∼16 hours) ...
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bioRxiv - Systems Biology 2024Quote: ... amplicons were indexed using xGen UDI Primer Pairs (10 mM) in 12 µL reactions using the NEB Q5 Hot Start HiFi 2X master mix (New England BioLabs, Ipswich, MA) with the following parameters ...
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bioRxiv - Genomics 2024Quote: ... DNA purified from the transposed product was then amplified via PCR (10 cycles) using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Cat. #M0491L).
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bioRxiv - Systems Biology 2022Quote: ... 100ng of vector backbone and 20ng of trcrRNA-CaptureSeq-5’LTR(truncated) cassette were combined with 10 μl of NEBuilder HiFi DNA assembly master mix (NEB cat. no. E2621L) and water to 20 μl ...
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bioRxiv - Genomics 2020Quote: ... 2 μg of total RNA from various tissues was used to construct RNA-seq library and sequence on the Illumina HiSeq X-10 platform following the protocol of NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs Ipswich, MA, USA).
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bioRxiv - Systems Biology 2020Quote: ... reaction components were added for second strand synthesis (70 μL water, 20 μL NEB second strand buffer, 10 μL NEB second strand enzyme). Second strand synthesis was carried out as described and the double-stranded cDNA was used as input for tagmentation ...
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bioRxiv - Biochemistry 2020Quote: ... A pre-cleaning step was applied by incubating each sample with 10 μl of Protein A magnetic beads (New England Biolabs GmbH, Frankfurt, Germany) for 1 h at 4 °C in tube roller (15 rpm ...
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bioRxiv - Molecular Biology 2020Quote: ... 25 µL of dA-tail reaction buffer and 10 µL of Klenow Fragment were added to the nuclei (both reagents were part of NEBNext dA-Tailing Module (NEB, #E6053L, Ispwich, MA)) ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 µ l of cfDNA was incubated at 37°C for 1 hour with the following reaction mixture: NEBuffer™ 2 (NEB, B7202), 0.25 mM MnCl2 (SIGMA ...
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bioRxiv - Microbiology 2022Quote: ... sequences of RcACE2 or hACE2 were PCR-amplified from pCAGGS-RcACE2- or pCAGGS-hACE2-expressing plasmids (10) and cloned into EcoRI- and XhoI-digested pCAGGS-blast plasmids using NEBuilder (New England Biolabs, Ipswich, MA, USA). Vero/TMPRSS2 cells were transfected with pCAGGS-blast-RcACE2 or pCAGGS-blast-hACE2 plasmids using the PEI MAX transfection reagent (Polysciences ...
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CRISPR-Cas9-mediated knockout of CYP79D1 and CYP79D2 in cassava attenuates toxic cyanogen productionbioRxiv - Plant Biology 2021Quote: ... The TD-PCR protocol began with an annealing temperature of Tm + 10 °C for the first cycling phase (Tm calculated by NEB Tm Calculator tool). The annealing temperature was then decreased by 1 °C per cycle until the primers’ Tm was reached ...
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bioRxiv - Molecular Biology 2020Quote: DNA adapters were prepared by resuspending complimentary oligos at a final concentration of 10 μM of each in CutSmart® buffer (New England Biolabs, Ipswich, USA). The reaction was then heated to 98°C for 2 minutes then set to ramp from 85°C to 65°C for 1 hour and finally from 65°C to 8°C for 30 minutes.
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bioRxiv - Biochemistry 2021Quote: ... 500 ng total of the purified PCR products were mixed with 1 μl 10× NEBuffer 2 (New England Biolabs Inc., Beverly, MA, USA) and ultrapure water to a final volume of 9.75 μl ...
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bioRxiv - Microbiology 2022Quote: ... Each sample was analyzed in duplicate 10-µl reactions containing 5 µl Luna Universal qPCR Master Mix (New England Biolabs, Ipswich, MA, USA), 2 µM of each primer ...
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bioRxiv - Molecular Biology 2019Quote: ... a volume of 0.5 µl of the respective restriction enzyme BbsI (5 units; ThermoFisherScientific, Waltham, US) or BsaI-HF®v2 (10 units; New England Biolabs, Ipswich, US) and then 1 µl (1-3 units ...
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bioRxiv - Cell Biology 2022Quote: ... 10 ng of pLenti6/V5-DEST™ vector was used as template per reaction with Q5® High-Fidelity DNA Polymerase (NEB, #M0491S). The PCR program ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA from each tail clip was standardized to 10 ng/µL and digested with the restriction endonuclease SbfI-HF (New England Biolabs, Ipswitch, MA, USA), and used to generate RAD-seq libraries33–35 ...
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bioRxiv - Microbiology 2022Quote: ... Nucleic acids cleavage was conducted at 37 °C for 10 min and stopped by addition of 2×RNA loading dye (New England Biolabs, Ipswich, MA, USA). Before loading ...
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bioRxiv - Zoology 2023Quote: Reverse transcription was conducted with 10 µL of eluted nucleic acids using the ProtoScript® II Reverse Transcriptase (New England Biolabs, Massachusetts, USA). For each sample ...
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bioRxiv - Cell Biology 2024Quote: ... and treated for 10 min at RT by RNase III enzyme (2 U of RNase III (Short Cut, New England BioLabs, Massachusetts, USA, M0245S) with 20 mM MnCl2 in 1× reaction ShortCut buffer) ...
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bioRxiv - Genomics 2024Quote: ... and half the elution was used as a template in a second round of 8-10 cycle PCR to prepare Illumina libraries using NEBNext multiplex oligos using the same conditions described above (NEB E7335L and E7500L). The final PCR product was purified using AMPure XP beads (Beckman Coulter A63882 ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragment release step was performed in 5 μl 1% SDS supplemented with 1:10 Thermolabile Proteinase K (New England Biolabs cat. no. P8111S) at 37°C 1 hr followed by 58°C 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragment release step was performed in 5 μl 1% SDS supplemented with 1:10 Thermolabile Proteinase K (New England Biolabs cat. no. P8111S) at 37°C 1 hr followed by 58°C 1 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... and produced by PCR amplification (10–13 cycles) of tagmented DNA using a NEB Next High-Fidelity 2× PCR Master Mix (New England Biolabs, Ipswich, MA, USA). DNA fragments were then purified using the MinElute PCR Purification Kit and eluted in 10 µL elution buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Washed Dynabeads containing the DNA-complexes were directly resuspended in 20 µL USER reaction buffer containing 10 µL StickTogether DNA Ligase Buffer 2x (NEB, cat. no. B0535S), 1.5 µL USER Enzyme (NEB ...
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bioRxiv - Microbiology 2024Quote: ... 10^106 of DMSO or IMP-1088 released virions were treated with two-fold dilutions of Furin (New England Biolabs, Cat. no. P8077L) in 20 mM HEPES ...
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bioRxiv - Bioengineering 2024Quote: ... along with a well containing 10 µl Quick-Load® Purple 1 kb DNA Ladder (New England Biolabs catalog no. N0552S; New England BioLabs, Ipswich, MA) and ran at 105 V for 45 minutes ...
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bioRxiv - Molecular Biology 2020Quote: Individual LAMP assays were assembled in a total volume of 25 μl of 1X Isothermal buffer (NEB; 20 mM Tris-HCl, 10 mM (NH4)2SO4 ...
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bioRxiv - Genomics 2019Quote: The genomic DNA was extracted from 10 mg frozen brain cortex samples using NEB Monarch genomic DNA® purification kit (NEB, Ipswich, MA, USA). Four microliters of RNase A (100mg/mL ...
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bioRxiv - Genetics 2021Quote: ... The col-10p::TIR1 line was generated by cloning to combine the col-10 promoter with the TIR1 construct of pDD356 (NEB Hifi Assembly Master Mix). Plasmid pAP082 was used to express Cas9 and a sgRNA to target the chromosome I insertion site ...
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bioRxiv - Biophysics 2019Quote: ... the imaging chamber was incubated in blocking buffer (10 mM Tris HCl pH 7.5, 50 mM NaCl, 1 mg/mL BSA [NEB], 1 mg/mL tRNA [Ambion]) for 1 h ...
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bioRxiv - Genetics 2024Quote: ... Cells were then pelleted by centrifugation at 500 x g for 10 min and washed with 200 µL of 1.5X DpnII reaction buffer (New England Biolabs Ipswich, MA cat no. R0543S), pelleted again and then resuspended in 300 µl of cold 1.5X DpnII reaction buffer (NEB cat no ...
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bioRxiv - Genomics 2023Quote: ... Lysed cells were then diluted 10 time in several tubes containing 450 μl of digestion mix (1X NEB 1 buffer, 1% Triton X-100). 100 units of HpaII were added and the tubes were incubated for 2 hours at 37°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... The Python script by default selects codons with the highest usage frequency for the input organism (E. coli NEB 10-Beta in this study), unless specified problematic sequences are introduced (Supplementary Figure S3B) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were further lysed with probe sonication and the lysate was denatured by adding 10 µL of denaturing buffer (NEB PNGase F kit, P0709) and heating at 100 °C for 5 min ...
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bioRxiv - Molecular Biology 2019Quote: ... in DMEM/F12 + 10% FBS medium were incubated with 20 μg PNGase F (self-prepared for flow cytometry, NEB Cat. No. P0704 for confocal microscopy) for 5 h at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... BAC baits were PCR amplified using 5’ biotinylated forward and reverse primers (Supplementary Table S7) in the following reaction mix: 10 µl 2X NEBNext® Ultra™ II Q5® Master Mix (NEB cat. M0544S), 1 µl 10 µM each bio-primer ...
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bioRxiv - Biochemistry 2024Quote: ... final concentration 30 nM) at 37 °C for 10 min before being added to M13mp18 single-stranded DNA (New England Biolabs, final concentration 25 ng/μL). Aliquots were quenched at the indicated time points by adding an equal volume of phenol-CHCl3-isoamyl alcohol and analyzed by agarose gel electrophoresis as described above.
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bioRxiv - Microbiology 2021Quote: ... 15 μL of PCR product were mixed with 5 μL of a 5X Exo-SAP solution (15% Shrimp Alkaline Phosphatase – 1000U/ ml – NEB, 10% Exonuclease I – 20000 U/ ml – NEB, 45% glycerol 80% and 30% dH2O). and incubated for 30 min at 37°C and for 15 min at 80°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’ triphosphates on the synthesis products were converted into 5’ monophosphates: samples were treated with recombinant shrimp alkaline phosphatase (NEB) (10 µl reaction in 1× NEB buffer 2.1, 37°C 30 min, 65°C 5 min), these reactions were made up to 15 µl including 0.5 µl 10× NEB 2.1 ...