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Citations for New England Biolabs :
3751 - 3800 of 4045 citations for 7 11 Dimethyldodeca 4 6 10 trien 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... then 10 μL of diluted cDNA was used with the NEB Luna Universal Probe qPCR Mastermix (New England BioLabs®) following the manufacturer’s protocol and RT-qPCR performed in 384-well plates using a QuantStudio™ 5 Real-Time PCR System (Applied Biosystems™) ...
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bioRxiv - Microbiology 2020Quote: ... each reaction tube of 20 μl contained 10 μl of Q5 High-Fidelity 2× Master Mix (New England BioLabs Inc.), 20 pmol of forward and reverse primer ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR of 10 cycles with an annealing temperature 61°C was performed using Q5 High-Fidelity DNA Polymerase (#M0491S, New England Biolabs) and BEL and AL primers on 5μL of I-SceI digested DNA purified from the ChIP procedure ...
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bioRxiv - Plant Biology 2020Quote: ... and an aliquot containing 10 µg protein was treated with lambda protein phosphatase reaction mix following the instructions of the manufacturer (New England Biolabs) for 1 h at 30°C.
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bioRxiv - Molecular Biology 2021Quote: ... 10 μL of eluted RNA was mixed with 1 μL of 200 ng/μL random nonamer primer (New England Biolabs), incubated at 65°C for 5 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... 0.3-kb promoter-xopL gene was PCR amplified using Xcv 85-10 genomic DNA as template and cloned into pBBR1MCS-2 (76) linearized with EcoRV (New England Biolabs) using Gibson assembly ...
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bioRxiv - Plant Biology 2021Quote: ... the 1.7-kb upstream and downstream regions of the xopL or xopQ gene were PCR amplified using Xcv 85-10 genomic DNA as template and cloned into pLVC18 linearized with EcoRI (New England Biolabs) using Gibson assembly ...
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bioRxiv - Neuroscience 2019Quote: ... Heated mixtures were cooled to room temperature for 10 min and 30 ng/µl EnGen Cas9 protein (New England Biolabs) was added ...
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bioRxiv - Biochemistry 2019Quote: ... Reactions were carried out in 10 μl of the purified components from the PURExpress In Vitro Protein Synthesis Kit (E6800S/L, NEB) with the corresponding templates ...
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bioRxiv - Biochemistry 2019Quote: ... The digested PCR product was circularized by T4 DNA ligase in a total volume of 500 μL 1x T4 DNA Ligase Reaction Buffer containing 100 μL digested PCR product and 10 kU T4 DNA Ligase (NEB). The reaction was incubated overnight at 16°C and purified by ethanol precipitation ...
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bioRxiv - Genomics 2021Quote: ... The PCR mix (8 µl H2O, 2 µl primer mix P5Solexa/P3Solexa, 10 µM each, 20 µl Phusion HF Mix [New England Biolabs]) was added to 10 µl cDNA ...
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bioRxiv - Genomics 2021Quote: ... and libraries were amplified by adding 10 μL DNA to 25 μL of NEBNext HiFi 2x PCR mix (New England Biolabs) and 2.5 μL of 25 μM each of Ad1 and Ad2 primers using 11 PCR cycles ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescent probes diluted in Hybridization Buffer (10% dextran sulfate, 0.1mg/ml salmon sperm ssDNA, 100 μl vanadyl ribonucleoside (NEB biolabs), 20ug/ml RNAse-free BSA ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mM of purified protein was combined with 2 mM DTT and 10 mM SNAP-Surface Alexa Fluor 546 (New England Biolabs) in PBS for 60 min at 30°C ...
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bioRxiv - Biochemistry 2021Quote: ... RNA (50 pmol) was radiolabelled at 5′ end with by using 10 units of T4 polynucleotide kinase (New England Biolabs) mixed to 3 μl of γ32P-ATP (3000 Ci/mmol 10 mCi/ml ...
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bioRxiv - Cell Biology 2019Quote: ... 95°C for 10 minutes and equal amounts of lysates (180 ug) were treated with either 1uL of EndoH (NEB), PNGase F (NEB) ...
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bioRxiv - Developmental Biology 2020Quote: ... Probes were diluted in Hybridization Buffer (10% dextran sulfate, 0.1mg/ml salmon sperm ssDNA, 100 µl vanadyl ribonucleoside (NEB biolabs), 20ug/ml RNAse-free BSA ...
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bioRxiv - Genomics 2021Quote: ... equal amount of naked DNA and DNA bound by Reb1 protein (~ 5pmol) were incubated with AAG (10 units) and APE1 (1 unit) (New England Biolabs) in a 20 μL reaction containing 1X Thermopol buffer (20mM Tris HCl pH 8.8 ...
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bioRxiv - Genomics 2020Quote: ... 25 uL of LNB buffer from LSK-109 kit (Oxford Nanopore Technologies) and 10 uL of ligase (New England Biolabs) and and 5 uL of adapter from LSK-109 kit was then added the reaction mixture and mixed by gentle tapping ...
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bioRxiv - Genomics 2020Quote: ... Each reaction is performed in 50 μl at 30°C overnight with 2 μl of circulated DNA with 2.5 μL of 10 μM (each) dNTPs (Catalog number: N0446S Vendor: New England Biolabs Inc), 2.5 μL Rolling cycle primer (10 μM) ...
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bioRxiv - Genomics 2020Quote: ... Zyagen samples were amplified with PBC096 barcoding for 8-10 cycles with both LongAmp (female, 62°C annealing; NEB, US) and PrimeSTAR GXL (male and female ...
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bioRxiv - Molecular Biology 2021Quote: The 5’ end phosphorylation was performed by treating 10 μg of total RNA extract with 0.5 μl of RNase inhibitor (20U final, NEB M0307L), 1 μl of 10x T4 PNK reaction buffer ...
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bioRxiv - Genomics 2019Quote: ... the fragmented genomic DNA was ligated with 1 µL of 10 µM phosphorylated hairpin oligo mix (1 µL of NEB T4 ligase, 1 µL of 10x NEB T4 Ligase buffer ...
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bioRxiv - Microbiology 2020Quote: ... using separate template dilutions (1:1 & 1:10) and high-fidelity Phusion polymerase (New England BioLabs Inc., Ipswich, MA, USA). A single round of PCR was performed using “fusion primers” (Illumina adaptors + indices + specific regions ...
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bioRxiv - Immunology 2020Quote: ... 70°C for 10s and 72°C for 30s) and 1x 72°C for 5 min) using the Q5 polymerase (New England BioLabs) and cloned into pUC57 using the MEGAWHOP35 method ...
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An egg-derived sulfated N-Acetyllactosamine glycan is an antigenic decoy of influenza virus vaccinesbioRxiv - Immunology 2021Quote: ... 25 ug of the 2020 QIV was denatured for 10 minutes at 75°C and treated with the Protein Deglycosylation Mix II (New England Biolabs) for 30 minutes at 25°C and 1 hour at 37°C ...
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bioRxiv - Genomics 2021Quote: ... beads were resuspended in 25 μl 0.5x TT and on bead PCR for addition of Illumina-specific adapters and 10-bp Unique Dual Indexes (UDIs) using NEBNext 2X High Fidelity PCR MM (NEB) and 25 PCR cycles was performed (Figure 5-table supplement 2) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 200 ng of DNA from each sample was digested with 10 U ApeKI (New England BioLabs Inc., Ipswich, MA, USA) for 2 h at 75 C ...
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bioRxiv - Microbiology 2020Quote: ... each reaction tube of 20 µl contained 10 µl of Q5 High-Fidelity 2× Master Mix (New England BioLabs Inc.), 20 pmol of forward and reverse primer ...
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bioRxiv - Microbiology 2022Quote: ... Radioactive probe was prepared using 40 pmol of primer 59 and 5’-labelled with 10 U of T4 Polynucleotide Kinase (New England Biolabs) and [γ32P]ATP (150 μCi) ...
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bioRxiv - Cell Biology 2022Quote: ... 10 µM Verapamil in DMSO (Spirochrome) and of 0.6 µM SNAP-Cell TMR-Star in DMSO (New England Biolabs (S9105S) for 120 mins as per manufacturers’ instructions ...
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bioRxiv - Cell Biology 2022Quote: ... genomic DNA (5-10 μg) derived from transfected ESC was digested by high concentrated HindIII-HF or high concentrated BamHI-HF (NEB). In all Southern blot analyses ...
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bioRxiv - Microbiology 2022Quote: ... and an equimolar mix of primers P5-IR2a-d primer (10 µM) in a reaction with 50 ng of adaptor ligated template and Phusion DNA polymerase (NEB) in a thermocycler with the following program 98°C 3 min ...
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bioRxiv - Genomics 2022Quote: ... the DNA was denatured and ligated to oligo ARC140 overnight at room temperature by 10 U T4 RNA ligase (New England Biolabs) in 25% PEG8000 and 1 mM CoCl3(NH3) ...
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bioRxiv - Microbiology 2022Quote: ... Institut Jacques Boy), recombinant human IL-2 (rhIL2, 10 U/ml, Miltenyi) and DNase (1 U/ml, New England Biolabs), washed and enumerated ...
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bioRxiv - Plant Biology 2022Quote: ... at 37 °C for 15 min followed immediately by 10 cycles of indexed PCR using NEBNext Ultra II Q5 DNA Polymerase (New England Biolabs) and Illumina’s PE primer set ...
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bioRxiv - Microbiology 2022Quote: ... The Guide RNAs were synthesised from Sigma and reconstituted by mixing 10 μM of the forward and reverse strands for each guide with 1 μl of 10X ligation buffer (NEB), 0.5 μl T4 polynucleotide kinase (NEB ...
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bioRxiv - Plant Biology 2022Quote: ... For quantitative PCR 90 ng of template DNA was used in 10 μl qPCR reactions with 0.25 μM primers and Luna qPCR Master Mix (NEB, USA). qPCR was performed in a LightCycler 480 (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... each 10 μL of double stranded PCR products were treated with 5 units Lambda exonuclease enzyme (New England Biolabs, NEB) at 37 °C for 45 min which is followed by 10 min at 75 °C for enzyme inactivation.
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bioRxiv - Molecular Biology 2023Quote: ... each 10 μL of double stranded PCR products were treated with 5 units Lambda exonuclease enzyme (New England Biolabs, NEB) at 37 °C for 45 min which is followed by 10 min at 75 °C for enzyme inactivation.
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bioRxiv - Molecular Biology 2023Quote: ... pre-existing SNAP-tagged histones were first quenched by incubating cells with 10 μM of the non-fluorescent SNAP reagent (SNAP-cell Block, New England Biolabs) for 30 min at 37°C followed by a 30 min wash in fresh medium and a 2-hour chase ...
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bioRxiv - Genomics 2022Quote: Add 5 μl of dNTP mix (10 mM each) and 5 μl of DNA polymerase I Klenow fragment (NEB, M0210) to the reaction system ...
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bioRxiv - Synthetic Biology 2022Quote: A double-stranded DNA scaffold template was obtained from pUCIDT-AMP-T7p_DB982 plasmid (10 ng in 50 μL reaction mixture) by PCR amplification using Phusion® DNA polymerase (NEB), T7-DB982 forward and T7-DB982 reverse primers (0.5 μM each) ...
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bioRxiv - Molecular Biology 2022Quote: 10 μg of >200nt RNA (in nuclease-free water) was decapped using 200 U yDcpS and yDcpS buffer (NEB, #M0463S) at 37°C in a thermomixer at 800rpm pulse shaking ...
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bioRxiv - Immunology 2023Quote: ... Completed libraries were then sequenced to an average depth of approximately 20M reads per sample on a partial lane of the NovaSeq6000 S4 XP flow cell using 2×150 paired-end reads with 10-base dual indexes (CAS: E6440S, NEB). After demultiplexing these samples ...
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bioRxiv - Molecular Biology 2024Quote: ... Each methylation reaction was thoroughly mixed on ice using 100uL of cell methylation buffer (10% volume 10x M.CviPI reaction buffer (NEB, B0227S), 300 mM sucrose ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... gDNA (10 µg) was digested overnight at 37°C with 100 U each of the restriction endonucleases MseI (NEB, R0525L) and BglII (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... Nicks were sealed by adding 10 μL 10x T4 DNA ligase buffer and 2 μL T4 DNA ligase (New England Biolabs) and incubating overnight at 16°C ...
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bioRxiv - Microbiology 2024Quote: ... The coverslips are then incubated for 30 min at 37°C with DMEM supplemented with 10% SVF containing 0.12 μM SNAP-Cell TMR-Star (New England Biolabs #S9105S), rinsed twice with PBS and incubated for 45 min with Dapi (1μg/ml) ...
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bioRxiv - Microbiology 2024Quote: ... The radioactive probe was prepared from 40 pmoles of D072 primer (Table 1) and labeled at the 5’ end with 10 units of T4 polynucleotide kinase (New England Biolabs) and [γ-32P]-ATP (150 μCi) ...