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Citations for New England Biolabs :
3951 - 4000 of 4045 citations for 7 11 Dimethyldodeca 4 6 10 trien 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Nascent RNA was then fragmented at 85C for 10 min and converted to cDNA using NEBNext Ultra II RNA First Strand Synthesis Module (NEB, E7771) and NEBNext Ultra II Directional Second Strand Synthesis Kit (NEB ...
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bioRxiv - Genomics 2023Quote: ... The pellet was resuspended in 90 µL of freshly prepared Micro-C “Master Mix 1” (10 μl 10x T4 DNA Ligase Buffer, 75 μl ddH2O, 5 μl T4 PNK (NEB #M0201L)) ...
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bioRxiv - Genomics 2023Quote: Restriction digestion was carried out by adding 25 μL of 10× CutSmart buffer and 100 U of the MluCI restriction enzyme (NEB #R0538) plus 100 U of the MboI enzyme (NEB #R0147) ...
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bioRxiv - Genomics 2023Quote: ... The reaction was diluted to 100 µL with 1X T4 PNK (polynucleotide kinase) buffer and incubated for 30 min with 1 µL T4 PNK (10 U/µL; NEB M0201) to remove 3’ phosphoryl groups ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were performed at 37°C for the indicated time (30 min if not otherwise specified) and then terminated by the addition of 10 μL of 2× RNA loading dye (New England Biolabs, USA). Reaction samples were heated at 85°C for 2 min ...
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bioRxiv - Biophysics 2023Quote: ... Trypsin digestion was initiated by adding 1:10 (trypsin to protein, m/m) mass spectrometry grade trypsin (New England Biolabs #P8101S) in 50 mM ammonium bicarbonate ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 µg of genomic DNA extracted using the Phenol-Chloroform method and treated with RNase A were digested with 10 units of DpnII (NEB, R0543S) in a final volume of 20 µL at 37°C for 6 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... ligated vectors were then transformed into NEB10-beta electroporation ultracompetent E.coli cells (NEB® 10-beta Electrocompetent E. coli, NEB, Ref.C3020K) and grown overnight on LB plates supplemented with 50 μg/mL Carbenicillin (Carbenicillin disodium salt ...
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bioRxiv - Microbiology 2024Quote: ... 2 μL template cDNA/DNA was used in a 20 μL reaction containing 10 μL Luna® Universal qPCR Master Mix (New England Biolabs). The qPCR program was ...
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bioRxiv - Genomics 2024Quote: ... of 0.1 μM polyT-rootV2 oligo diluted in hybridization buffer (2x SSC, 1 mg/mL yeast RNA, 10% dextran sulfate, 1:1000 Murine RNase inhibitor NEB M0314) on a parafilm sheet placed on a glass plate ...
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bioRxiv - Evolutionary Biology 2024Quote: ... ddRAD library preparation included an initial digestion of 300 ng of DNA in a 34 μL reaction (2 hours at 37 °C; 10 U each of SbfI and MspI, New England Biolabs Inc.). Standard Illumina adapters were ligated using 60 cycles of digestion at 37 °C (2 minutes ...
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bioRxiv - Molecular Biology 2024Quote: dsBC was cloned into the digested vector by combining 200ng vector (∼0.035pmol, 8uL), 10ng dsBC (∼0.175pmol, 2uL) and 10 μL 2x NEBuilder® HiFi DNA Assembly Master Mix (NEB cat#E2621L) and incubating at 50°C for 1hr ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Whole-cell extracts were quantified using the BCA assay and 10 µg was subjected to PNGase F (New England Biolabs, #P0705S) treatment according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2022Quote: ... of CROPseq-CaptureSeq-Guide-Puro backbone and 400 fmoles (18.31ng) of amplified dsDNA oligonucleotides were combined with 10 μl of NEBuilder HiFi DNA assembly master mix (NEB cat. no. E2621L) and water to 20 μl ...
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bioRxiv - Physiology 2020Quote: RNA was extracted from cultured cells or tissue (∼10 mg) stored in RNALater® using Monarch® Total RNA Miniprep Kit (New England BioLabs). For maximal RNA recovery ...
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bioRxiv - Genetics 2020Quote: For Southern blot analysis 150 ng of mouse DNA was digested with 10 units of BamHI-HF (NEB, Figure 2 and 5), NdeI (NEB ...
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bioRxiv - Developmental Biology 2019Quote: ... Total RNA was then treated with DNase for 10 min at 37 °C to remove genomic DNA [10 μg RNA in 100 μl reaction buffer with 2U DNase I from NEB (Ipswich, MA)] followed by phenol-chloroform extraction and ethanol precipitation.
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bioRxiv - Genomics 2019Quote: ... PCR reactions for library preparation were performed by adding the following to the 10 μL DNA sample: 25 μL of Phusion High-fidelity PCR Master Mix (NEB catalog # M0531S), 1 μL of 5’ library index barcode ...
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bioRxiv - Plant Biology 2021Quote: ... The F1 fragment of eYGFPuv was assembled by cloning the gblocks and a PCR-amplified relevant fragment into an eYGFPuv vector 10 through NEBuilder HiFi DNA Assembly (New England BioLabs, Catalog #E5520S). Similarly ...
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bioRxiv - Microbiology 2021Quote: ... were cloned into the pCR-Blunt-II vector using the PCR Blunt Topo kit according to the manufacturer’s instructions and transformed into 10-beta CaCl2-chemically competent bacteria (originally NEB, generated in-house). Following amplifications of colonies and purification of plasmids using a QiAprep Mini kit (Qiagen) ...
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bioRxiv - Physiology 2022Quote: ... from epithelium or sensory neurons underwent digestion for 10 hours at 37°C with MspI restriction enzyme (New England Biolabs, Ipswich, MA), followed by purification with AMPure XP (Beckman Coulter ...
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bioRxiv - Molecular Biology 2020Quote: ... and 3 μL of factor mix (with RNA polymerase, and transcription/translation factors in 10 mM Mg2+) from the PURExpress® Δ Ribosome Kit (New England Biolabs). The reaction buffer was based on Shimizu et al ...
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bioRxiv - Microbiology 2021Quote: ... Triplicate NEBuilder HiFi assembly reactions were prepared according to manufacturer’s protocols containing ∼2:1 insert to vector as follows: 10 µl of 2X NEBuilder HiFi master mix (New England Biolabs, cat#E2621S), 158.7 ng of DNA fragments ...
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bioRxiv - Biophysics 2021Quote: ... The surface is then passivated by incubating bovine serum albumin (BSA, New England Biolabs, 10 mg/ml in PBS and 50% glycerol) for 30 minutes ...
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bioRxiv - Synthetic Biology 2020Quote: ... were PCR amplified from genomic DNA of yeast strain BY474129 and cloned together with the bidirectional inducible Gal1-10 promoter into the PCR amplified pRSII42B backbone with OZL14 and OZL15 via Gibson assembly30 using NEBuilder Kit (NEB, catalog E2621). MMR-DN mutant genes were subsequently generated via site-directed mutagenesis using either Q5 Site-Directed Mutagenesis Kit (NEB ...
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bioRxiv - Genomics 2020Quote: ... and 10-12 day adult TRAP libraries were prepared with the NEBNext Ultra RNA library Prep Kit for Illumina (NEB, product E7530S). ChIP-seq libraries were prepared using the NEBNext ChIP-seq Library Prep Master Mix Set for Illumina (NEB ...
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bioRxiv - Plant Biology 2020Quote: ... The suitability of the selected restriction enzyme pair was confirmed by digesting 400 ng of genomic DNA using 5 Units of each restriction enzyme and NEB CutSmart™ buffer (10×) (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Cancer Biology 2020Quote: ... Up to 10 ng of cDNA was used for the Illumina sequencing library construction using NEBNext® Ultra™ DNA Library Prep Kit (NEB). Paired ends sequencing was performed on NextSeq 500 (Illumina ...
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bioRxiv - Plant Biology 2020Quote: ... and the quality was checked by digestion of 250 ng of genomic DNA from 10 random samples with the restriction enzyme EcoRI (New England Biolabs, Boston, MA) at 65°C for 2 hour and thereafter visualised on agarose gel.
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bioRxiv - Zoology 2019Quote: ... PCR reactions were performed in a total volume of 50 µL containing 10 µL NEB 5X Q5® Buffer (New England BioLabs, USA), 10 µL NEB 5X Q5® High GC Enhancer (New England BioLabs ...
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bioRxiv - Genomics 2020Quote: ... and PCR enrichment of adapter-ligated DNA was performed for 10 cycles using NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs). Amplified libraries were purified with 0.7x SPRIselect beads and sequenced with MiSeq Reagent Kit v3 ...
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bioRxiv - Genetics 2020Quote: ... Zebrafish embryos were injected at the 1-cell stage with a 1 nL solution of bbs2 gRNA (200 ng/μL) and Cas9 protein (10 μM; New England BioLabs, Beverly, MA). Mutagenesis was confirmed by High Resolution Melt Analysis (HRMA ...
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bioRxiv - Microbiology 2021Quote: ... Pooled samples were clarified by centrifugation (10 000 g, 15 min) and treated with DNase I and RNase If (NEB, Great Britain) for removing of naked out-capsid nucleic acid ...
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bioRxiv - Developmental Biology 2022Quote: ... Four µl cDNA was used as input for 10 µl qPCR reactions with 2x Luna Universal qPCR Master Mix (NEB cat. #M3003X) and 0.25 µM forward and reverse primers (Extended Data Table 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Adapter ligation was performed at 20°C for 10 min, with Adapter Mix (AMX, ONT) and Blunt/TA Ligase Master Mix (New England BioLabs, cat# M0367). After a final 1X clean-up and washing of the beads with Adapter Binding Buffer (ABB ...
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bioRxiv - Microbiology 2022Quote: ... immitis and Wolbachia qPCR assays were carried out in 10 μL final volumes of Luna® Universal Probe qPCR Master Mix (New England Biolabs, Australia) containing 1 μL of template DNA isolated from whole dog blood and primers and probe at a final concentration of 400 nM and 100 nM ...
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bioRxiv - Developmental Biology 2024Quote: ... The RNA was then treated with DNase for 10 min at 37◦C to remove genomic DNA [10 μg RNA in 100 μl reaction buffer with 2U DNase I from NEB (Ipswich, MA)] followed by phenol-chloroform extraction and ethanol precipitation ...
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bioRxiv - Genomics 2024Quote: ... after which 68 µL RNase-free H2O was added with 10 µL DNase I Buffer and 2 µL DNase I (New England Biolabs, Ipswitch, MA). The resulting mix was incubated for 15m at 37°C ...
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bioRxiv - Genomics 2024Quote: The custom double stranded adapter containing primer-binding site was ligated by mixing 10 μl of NEB TA/Blunt Ligase Master Mix (cat #M0367, New England Biolabs MA, USA), 2 μl of duplex adapter (1 μM ...
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bioRxiv - Molecular Biology 2023Quote: ... and pACG9-[EC1-10]) were generated through site-directed mutagenesis (NEB Q5 site-directed mutagenesis kit; New England Biolabs, Ipswich, MA, USA). Specifically ...
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bioRxiv - Genetics 2023Quote: ... One microliter of T-tailed DNA adaptors diluted to 1.5 µM in water was added to 5 µl of amplicons diluted to 1/10 in water and 5 µl of 2X Blunt/TA Ligase Master Mix (New England Biolabs, Herts, UK) and incubated 30 min at 25°C for ligation ...
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bioRxiv - Microbiology 2023Quote: ... Radiolabeling of 50 pmol of RNA substrate was performed in 20 μl of reaction mix using 10 U of T4 PNK (New England Biolabs, cat. M0201) and 3μL of ?32P-ATP (Perkin Elmer ...
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bioRxiv - Microbiology 2023Quote: ... and PPT1 gene deletion constructs were made by assembling the recyclable cassette from pBV65 (10) and fragments from the immediate upstream/ downstream regions of each gene by Gibson assembly cloning (New England Biolabs, Ipswich, MA). Eviction of the recyclable cassette left a single copy of loxP in place of the removed target gene coding region ...
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bioRxiv - Genomics 2022Quote: ... was diluted to a concentration of 10 μM and combined with HiFi Cas9 Nuclease V3 (IDT, cat 1081060) in CutSmart Buffer (NEB, cat B7204). Cas9 cleavage and library preparation was performed in accordance with previously described methods[23] ...
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bioRxiv - Genomics 2023Quote: ... restriction enzyme (10 units of HpyCH4IV [NEB] for PCR7 and PCR31, and 20 units of TaqI-v2 [NEB] for PCR11 and PCR12), 1 μL of 10X CutSmart buffer (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... was immersed in a 10-fold volume of digestion solution with 8 U/mL Proteinase K (P8107S, New England Biolabs, Ipswich, USA) in digestion buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Developmental Biology 2023Quote: ... and then hybridized overnight at 37°C in hybridization buffer (2xSSC, 10% dextran sulfate [Sigma-Aldrich, D8906], 1mg/mL yeast tRNA [Sigma-Aldrich, R8759], 2mM Vanadyl Ribonucleoside complex [New England Biolabs, S1402S] ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we purified the products of the ace-1 PCR (see above) using the BS664-250 Preps EZ-10 Spin Column PCR Purification kit (New England BioLabs, Evry France). The purified PCR products were then cloned (TOPO TA Cloning Kit pCR 2.1-TOPO Vector and TOP10F’ invitrogen bacteria) ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA libraries were prepared from 50 ng (hippocampi) or 10 ng (amygdalae) RNA using the NEBNext® Ultra™ II RNA Library Prep Kits for Illumina (New England Biolabs). Libraries were sequenced (40M bp sequencing depth ...
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bioRxiv - Microbiology 2023Quote: ... A total of 3 μg of RNA was denatured at 65°C for 10 min with equal volume of RNA loading dye (New England Biolabs, MA, USA). RNA samples ...