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Citations for New England Biolabs :
3601 - 3650 of 4045 citations for 7 11 Dimethyldodeca 4 6 10 trien 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... 96-well plates were centrifuged again at 3000 x g for 10 min and 1 µL of the supernatant was used in 12.5 µL PCR reactions with Taq polymerase (NEB) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was passed three times over a column of 10 ml of amylose resin (New England Biolabs, E8031L). The column was washed with MBP wash buffer (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... The pellet was resuspended in 18.5 μL T4 DNA Ligase Reaction Buffer with 5 μL of 10 μM annealed Broken end linker and 1.5 μL T4 DNA Ligase (#M0202S, New England Biolabs). The reaction was incubated 18-20 h at 16°C with constant mixing ...
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bioRxiv - Neuroscience 2022Quote: ... we added 1μL unique barcoded N5&N7 (0.5 µM final concentration) and 10 μL NEBNext High-Fidelity 2x PCR Master Mix (NEB). PCR cycling conditions were as follow ...
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bioRxiv - Biochemistry 2022Quote: ... 1× CutSmart buffer (50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate,100 μg/mL BSA, pH 7.9, NEB), 20 U RiboLock ...
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bioRxiv - Genomics 2022Quote: ... 10 ng DNA was used to prepare sequencing libraries with the Ultra II DNA Library Prep kit (NEB #E7645S).
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was cut into pieces and PK buffer (100 mM Tris pH 7.4, 50 mM NaCl, 10 mM EDTA) was added together with Proteinase K (NEB). After 90 min of incubation at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... synthetic dsDNA target oligos were prepared by hybridizing 10 μM of ssDNA template with 50 μM of complementary ssDNA in 1x NEBuffer 2.1 (NEB), to provide a solution with an effective dsDNA concentration of 10 μM ...
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bioRxiv - Developmental Biology 2022Quote: ... dissected wing imaginal discs were incubated for 10 min at 25°C with SNAP-Surface Alexa Fluor 546 (3.3 μM, from NEB), rinsed and incubated for 15 min with SNAP-Surface Block at 13 μM before fixation and immunolabeling ...
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bioRxiv - Genomics 2021Quote: ... We amplified libraries by adding 10 ul DNA to 25 ul NEBNext HiFi 2x PCR mix (New England Biolabs) and 2.5 ul of a 25 uM solution of each of the Ad1 and Ad2 primers ...
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bioRxiv - Evolutionary Biology 2021Quote: DNA was isolated from single fins by placing them in lysis buffer (10mM Tris pH 8, 100mM NaCl, 10 mM EDTA, 0.5% SDS) with Proteinase K (333µg/mL, NEB P8107S) at 55°C for between four hours and overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... DNase treatment of indicated samples was carried out by incubation of each sample with 10 μl of DNaseI (NEB), 5 μl of Benzonase Nuclease (Novagen ...
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bioRxiv - Genetics 2020Quote: ... 10 μl of 20 ng/ul DNA per individual was fragmented using the high-fidelity enzymes NsiI / MspI (NEB) combined in a double digest ...
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bioRxiv - Microbiology 2021Quote: ... 40 μL of 10 μM FISH probes in hybridization buffer (10% dextran sulfate [Sigma], 2mM vanadyl ribonucleoside complexes [#514025, NEB], 0.02% RNAse-free BSA [NEB] ...
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bioRxiv - Microbiology 2022Quote: ... Reverse transcription of 10 µl RNA was performed using LunaScript RT Supermix Kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The total volume reaction was 25 μl with the following composition: 5 μL 10× buffer at 1.0 mM (New England BioLabs), 0.1 mM each dNTP ...
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bioRxiv - Biochemistry 2022Quote: ... The N-linked glycans and the CBP tag were cleaved by endoglycosidase H (Endo H, ∼0.2 mg per 10–15 mg purified protein, New England Biolabs) and HRV-3C protease (∼2 mg per 10–15 mg purified proteins ...
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bioRxiv - Genetics 2019Quote: ... 10 pmoles of forward and universal reverse primers were annealed and extended with Q5 high-fidelity DNA polymerase (NEB) according to the recommended conditions and with the following program ...
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bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl) and incubated for 10 min at 37 °C with 2.000 units/mL of DNase I (New England BioLabs). Half of the resulting sample was incubated with 10 µg/mL of PK (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by 10 µl of 10X NEB2 buffer and 15 µl of HindIII (New England Biolabs, 20 U/µl) to digest at 37°C for 2 hr ...
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bioRxiv - Synthetic Biology 2019Quote: ... The solution was then equilibrated to 42°C for 10 min before adding 2 μL of β-agarase (NEB). Finally ...
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bioRxiv - Pathology 2019Quote: ... by adding a mixture of 10 µl DNase I and 190 µl DNase I Reaction Buffer (New England Biolabs) to the priming port and incubated for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... in a total volume of 20 µL containing 10 U of T4 RNA Ligase 1 (NEB, cat n° M0204L), 1X T4 RNA ligase buffer ...
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bioRxiv - Synthetic Biology 2021Quote: ... the IVT template RNA (0.01– 0.05 A260 units) was digested into nucleosides using 10 µg/ml nuclease P1 (New England Biolabs, M0660S), and 0.5 U/ml Bacterial Alkaline phosphatase (Takara 2120A ...
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bioRxiv - Molecular Biology 2021Quote: ... The pellet was resuspended in 88 μL of NEBuffer 2.1 with 10 μL dNTP (1mM, #N0446S, New England Biolabs) and 2 μL T4 DNA Polymerase (#M0203S ...
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bioRxiv - Bioengineering 2021Quote: ... 50 ng input genomic DNA was first linearly pre-amplified with 10 nM final concentration 5p-CCR5_UMI primer using the Q5 High-Fidelity DNA Polymerase (New England Biolabs): (98 °C ...
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bioRxiv - Genetics 2019Quote: ... followed by 10 μl of 10X NEB2 buffer and 15 μl of HindIII (New England Biolabs, 20 U/μl) to digest at 37°C for 2 hr ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized from total RNA of 10-d-old seedlings using M-MuLV reverse transcriptase (New England Biolabs); each gene was amplified using specific primers (see Supplemental Table 2 ...
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bioRxiv - Genomics 2021Quote: ... Ligation was done overnight at 16°C also rotating at 900rpm for 10 seconds every 5 minutes by adding 120μl of 10X ligation buffer (NEB), 664μl water ...
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bioRxiv - Biochemistry 2021Quote: ... The mixture was incubated at room temperature for 10 min followed by transformation into 5-alpha competent cells (NEB). The E ...
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bioRxiv - Developmental Biology 2022Quote: ... Ligation was done overnight at 16°C also rotating at 900rpm for 10 seconds every 5 minutes by adding 120µl of 10X ligation buffer (NEB), 664µl water ...
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bioRxiv - Microbiology 2021Quote: ... Chromosomal DNA (1 µg) was digested with 10 units (1 µl) of restriction enzyme AciI (New England Biolabs (NEB)) for one hour at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Chromosomal DNA (1 µg) was digested with 10 units (1 µl) of restriction enzyme AciI (New England Biolabs (NEB)) for one hour at 37°C ...
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bioRxiv - Genomics 2020Quote: ... 200–300 ng/µL of high molecular weight DNA underwent single-strand nicking with 10 units of Nt.BspQI nickase (New England BioLabs). Nicked sites were repaired with fluorophore-labeled nucleotide to restore strand integrity.
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bioRxiv - Immunology 2021Quote: ... except after the Ni-NTA step it was concentrated (1 mg/ml in 0.2 ml) and shaved with endoglycosidase D (10 μl, 100 unit) (New England BioLabs) for 16 hrs at 4°C prior to gel filtration ...
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bioRxiv - Genetics 2019Quote: ... Genomic DNA was digested with 2.0 units of the BsaXI enzyme ((N)9AC(N)5CTCC(N)10) (New England Biolabs) and 2.0 units of the CspCI enzyme ((N)10-11CAA(N)5GTGG(N)12-13 ...
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bioRxiv - Genomics 2021Quote: ... the Hi-C library was amplified for about 10 cycles of PCR with the Q5 master mix (NEB, M0492L), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 10 or 20 minutes at 37°C and immediately quenched with 8μL 6x NEB purple loading dye (NEB B7024S) supplemented with 6mM EDTA (to make the total EDTA concentration in the stock tube 66mM) ...
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bioRxiv - Molecular Biology 2022Quote: ... and a PCR with 10-13 cycles was performed using the NEBNext High Fidelity 2X PCR Master Mix (NEB) and Ad1_noMX and Ad2.1–2.12 barcoded primers described in (30) ...
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bioRxiv - Bioengineering 2022Quote: ... RNA was isolated by precipitation in isopropanol-ethanol 75% and resuspended in 10 μL nuclease free water (NEB, B1500) before quantification by BioDrop μLITE (Biodrop) ...
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bioRxiv - Biochemistry 2022Quote: ... were assembled using 10 μl of 2× master mix at 50 °C for 1 h according to manufacturer’s instructions (NEB). 5α Competent Escherichia coli (30 μl ...
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bioRxiv - Genomics 2022Quote: ... 10 min) and resuspended in 400uL Buffer M supplemented with 1 mM S-adenosyl-methionine (SAM, New England Biolabs) and 200uL aliquoted as a non-methylated control ...
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bioRxiv - Immunology 2022Quote: ... 10 μg CAR-encoding vectors and 3.3 μg of each 3rd generation lentivirus helper vectors (CART-027CL, Creative-Biolabs). Supernatant media containing lentiviral particles was harvested 48 hours after transfection and used fresh or stored at −80 °C ...
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bioRxiv - Microbiology 2022Quote: ... 30 nM EnGen LbCas12a was pre-incubated with 30 nM crRNA in NEBuffer 2.1 for 10 minutes at 25°C. Substrate DNA (i.e. dsDNA template, M13mp18 ssDNA (NEB #N4040S) or purified Pf4 DNA ...
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bioRxiv - Microbiology 2022Quote: ... This was then incubated at 37 °C for 1.5 h with 50 μL of 10× CutSmart Buffer (New England BioLabs) supplemented with 5 mM CaCl2 ...
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bioRxiv - Microbiology 2022Quote: ... washing and TRIzol extracted followed by removal of the 5’ cap with 10 U of 5’-pyrophosphohydrolase (RppH) (NEB) and 5’ end repair with T4 PNK (NEB) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 x 106 PDCL-1108 cells were electroporated with 10 μg spCas9-NLS protein (New England Biolabs, Ipswich, MA), 4 μg guide RNA and 200 pmol ssODN using the Amaxa Nucleofector II (Lonza ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μL i7 unique index primer (10 μM) and 25 μL NEBNext High-Fidelity 2X PCR Master Mix (NEB) were added to 21 μl of purified CUT&TAG DNA ...
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bioRxiv - Microbiology 2024Quote: ... 1 µl of the annealed oligos were phosphorylated in a 10 µl reaction containing 0.25 µl T4 PNK (10000 units/ml, NEB) and 1 µl 10x T4 ligase buffer for 40 min at 37°Ϲ before heat-inactivation at 65°Ϲ for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... a master mix containing hydrogel beads carrying DNA stub and ligation mixture was distributed in the same 4 x 96-well plates such that each well would contain 12.5 μl of close-packed hydrogel beads,1.25 µl of 10× ligation buffer (NEB, B0202S), 0.3 μl of T4 DNA ligase I (NEB ...