Labshake search
Citations for New England Biolabs :
3951 - 4000 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Washed Dynabeads containing the DNA-complexes were directly resuspended in 20 µL USER reaction buffer containing 10 µL StickTogether DNA Ligase Buffer 2x (NEB, cat. no. B0535S), 1.5 µL USER Enzyme (NEB ...
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bioRxiv - Genomics 2023Quote: ... The strain CBS 411.78-was prepared and sequenced with the ligation kit SQK108 in a one-pot reaction using 500 ng DNA for end prep and ligation (NEB Ultra-II ligase) and sequenced on an R9.4.1 flowcell ...
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bioRxiv - Microbiology 2023Quote: ... We then used a 2:1 insert to vector ratio in a 1 hour HiFi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). HiFi assembly products were Ampure bead purified and eluted into 20uL of H2O for higher electroporation efficiency.
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bioRxiv - Genomics 2023Quote: ... using the Adapter Mix II from the Native Barcoding Expansion 1–12 kit and NEBNext Quick Ligation Reaction Buffer (New England Biolabs, Ipswich, MA, USA) as well as Quick T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Neuroscience 2024Quote: ... Each reaction was performed in a 10 μL volume containing 1× Luna Universal One-Step Reaction Mix plus 1× Luna WarmStart® RT Enzyme Mix (Cat. No. E3005; New England BioLabs® Inc.), 0.4 µM of each primer and 2 µL of 10 ng/µL RNA ...
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bioRxiv - Molecular Biology 2024Quote: All PCR assays for amplification of repair templates were performed in a 50 μl reaction volume using Q5 High-Fidelity 2X MasterMix (New England Biolabs NEB Cat #M0515) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... the RNA pellet was resuspended in 3’ ligation reaction buffer 1x and subjected to library preparation using the NEBNext® Small RNA Library Prep Set for Illumina® (NEB, #E7330S). Specific primers for V ...
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bioRxiv - Molecular Biology 2024Quote: ... DNaseI-treated 1 µg RNA aliquots were immediately used in cDNA synthesis and no-reverse transcriptase control (no-RT) reactions with random primers (New England BioLabs Protoscript II kit).
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bioRxiv - Molecular Biology 2024Quote: ... was generated by reverse transcriptase polymerase chain reaction (PCR) using the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, Ipswich, MA). Transcript levels were measured by real time PCR using SYBR green on an ABI 7500 system ...
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bioRxiv - Molecular Biology 2024Quote: ... and used as templates for in vitro transcription (IVT) reaction by the T7 promoter using T7 polymerase and the HiScribe T7 High Yield RNA Synthesis kit (NEB, cat. no. E2040L). Template DNAs were removed by treating with DNase I at 37°C for 30 minutes and the IVT RNAs were further purified by RNA purification kit (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... The PCR amplification was conducted in a 25 µl reaction volume using Q5® Hot Start High-Fidelity Master Mix (NEB, Cat. #M0494) using recommended primer concentrations of 0.5 µM each ...
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bioRxiv - Genomics 2024Quote: ... We employed the Luna Universal One-Step RT-qPCR Kit for the qPCR reactions (New England Biolabs, Ipswich, MA, USA, Catalog No. E3005). Each 20 μL reaction consisted of 10 μL reaction mix ...
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bioRxiv - Biochemistry 2024Quote: ... R3104L) for SMG6-3xFLAG in reactions containing 40 ng/ μl DNA (4 μg in total) in 1x CutSmart buffer (NEB, Cat No. B7204S) for 2h or overnight at 37°C ...
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bioRxiv - Neuroscience 2024Quote: All site-directed mutagenesis (SDM) reactions were done with the Q5 Site-Directed Mutagenesis Kit (catalog number E0554S, New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol and sequence verified by Sanger sequencing ...
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bioRxiv - Genomics 2024Quote: ... Transformation reactions were then transferred to deepwell 96-well plates containing room temperature NEB SOC Outgrowth Media (New England Biolabs, Ipswich, MA, USA), and incubated for 1 hour at 37°C with shaking at 800 rpm ...
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bioRxiv - Genomics 2024Quote: ... 1 µl of each enzyme cocktail component was added to the DNA sample and the digestion reaction was incubated at 37°C for 30 min in 1x rCutSmart Buffer (NEB, catalog number: E6004). Water was added to the untreated control for the digestion reaction ...
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bioRxiv - Plant Biology 2024Quote: ... to create a level-1 plasmid by cut and ligate reaction using BsaI restriction enzyme and T4 DNA ligase from NEB (New England Biosciences). All plasmids were confirmed by restriction digestion and DNA sequencing ...
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bioRxiv - Cancer Biology 2024Quote: ... were ligated to the DNA fragments in 50 µl of the Quick ligation mix containing 25 µl of 2x Quick Ligase reaction buffer (NEB, cat. no. B2200S) and 2 µl of T4 DNA ligase (2,000,000 U/ml ...
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bioRxiv - Zoology 2023Quote: Reverse transcription was conducted with 10 µL of eluted nucleic acids using the ProtoScript® II Reverse Transcriptase (New England Biolabs, Massachusetts, USA). For each sample ...
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bioRxiv - Microbiology 2024Quote: Reverse transcription was carried out with 8 μl of total nucleic acid added to 2 μl of NEB Lunascript RT Supermix (New England Biolabs Cat no. E3010). Nuclease free water was used as a no template control (NTC ...
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bioRxiv - Cell Biology 2024Quote: ... was replaced with a ten amino acid sequence from CNGB1 (PNPEEAETES) using the Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich, MA, USA).
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bioRxiv - Genomics 2020Quote: ... The chromatin pellet was resuspended in 2500 μL ligation reaction buffer (1x NEB Ligase buffer, 1x NEB BSA, 12500 U NEB T4 Ligase (NEB, M0202L)) and incubated rotating at room temperature for 2.5-3 hours ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 µl of the dissolved cells were added to a 50 µl PCR reaction (Q5 DNA polymerase, NEB; see Table S2 for primer sequences) and amplified with 36 rounds of thermal cycling ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was mixed with Reaction Mix 1 (9:1 of 12 uM NEB RT random 6N-S to 12 uM oligo-dT(20), 12uM dNTPs ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing adapters were ligated to the libraries using NEBNext Quick Ligation Reaction Buffer/Quick T4 DNA Ligase (New England Biolabs Inc., Ipswich, United States); ONT Short Fragment Buffer was used during the final library wash to retain fragments of all lengths ...
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bioRxiv - Plant Biology 2021Quote: ... the PCR reaction profile and program were setup as above with the following modifications: Q5 high-fidelity DNA polymerase (NEB, Frankfurt am Main, Germany) and 5x Q5 reaction buffer were used for PCR amplification ...
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bioRxiv - Genomics 2020Quote: Dialyzed chromatin was utilized as input (1.5 ug) for methylation reactions with the non-specific adenine EcoGII methyltransferase (New England Biolabs, high concentration stock 2.5e4U/mL). Reactions were performed in a 200uL reaction with 1X CutSmart Buffer and 1mM S-adenosyl-methionine (SAM ...
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bioRxiv - Genetics 2022Quote: ... In vitro transcription was performed in 20 µL reaction volume using HiScribe™ T7 High Yield RNA Synthesis Kit (New England BioLabs, catalog number E2040S) following the Capped RNA Synthesis protocol provided by the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... the internal primers contain complementary 30 nucleotides of the respective expression vectors in order to be ligated by Gibson reaction using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs, catalog no. E2621L). Ligated products were transformed into One Shot Stbl3 competent E ...
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bioRxiv - Microbiology 2021Quote: ... The PCR amplification was carried out with Phusion High-Fidelity PCR Master Mix with HF Buffer in 50uL reactions with 20ng of genomic DNA (New England Biolabs Inc., Ipswich, MA, USA). Reactions were run in an Agilent SureCycler 8800 (Agilent Technologies ...
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bioRxiv - Genetics 2021Quote: ... 5 μg of MBP-Rif2 or MBP-Rif2-min (see recombinant protein preparation) was incubated for 1 h at 4 °C with amylose resin (New England Biolabs, 50 μl per reaction). The resin with the immobilized MBP-Rif2 variants was washed 5 times with 1 ml wash buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Bioengineering 2020Quote: ... 2–3 μl of genomic DNA was used as a template in a 50-μL PCR reaction with Q5® High-Fidelity 2X Master Mix (NEB, Ipswich, MA, USA). The following primers (Table S1 ...
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bioRxiv - Molecular Biology 2021Quote: ... sequence of iRFP670 amplified from piRFP670-N1 vector by polymerase chain reaction (PCR) using Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA) and primers encoding NcoI and XmaI digestion sites (Supplementary Table S1) ...
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bioRxiv - Bioengineering 2022Quote: ... 1ul of genomic DNA was used as a template in a 20ul PCR reaction using Q5 HotStart DNA polymerase (NEB, Cat. No./ID: M0493L). Each individual was genotyped for the presence of the y-chromosome (42) ...
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bioRxiv - Bioengineering 2022Quote: ... 1 µl of genomic DNA was used as a template in a 20 µl PCR reaction using Q5 HotStart DNA polymerase (NEB, Cat. No./ID: M0493L) and primers 1154A.S5 and 1154A.S7 amplifying genomic Fle sequences ...
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bioRxiv - Genetics 2024Quote: ... Cells were then pelleted by centrifugation at 500 x g for 10 min and washed with 200 µL of 1.5X DpnII reaction buffer (New England Biolabs Ipswich, MA cat no. R0543S), pelleted again and then resuspended in 300 µl of cold 1.5X DpnII reaction buffer (NEB cat no ...
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bioRxiv - Developmental Biology 2023Quote: ... UAS expression constructs were generated by combining the linearized pGW-HA.attB backbone and each individual ZIKV fragment in Gibson assembly reactions (NEB Gibson Assembly Cloning Kit, E5510S). To generate NS4A::2K ...
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bioRxiv - Genetics 2023Quote: ... The oligonucleotides were annealed and subcloned into the Csy4-spCas9 vector plasmid (pCas9Pvcsg) using the Golden Gate reaction with restriction endonuclease BsaI-HF v2 (New England BioLabs, Ipswich, MA, catalog #R3733L). The expression of Csy4 ...
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bioRxiv - Immunology 2023Quote: ... was generated by reverse transcriptase polymerase chain reaction (PCR) using the ProtoScript® II First Strand cDNA Synthesis Kit (New England Biolabs, Ipswich, MA, USA). Transcript levels were measured by real time PCR using SYBR green on an ABI 7500 system ...
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bioRxiv - Bioengineering 2024Quote: ... The plasmid was directly amplified using Q5 polymerase using Polymerase chain reaction (PCR) (Q5® High-Fidelity PCR Kit, New England Biolabs, Ipswich, MA, USA). PCR was carried out following manufacturer protocol (Table S3 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and SSU rRNA genes were PCR amplified in 25 µl total reaction volumes using Q5® High-Fidelity DNA Polymerase (2x master mix, New England Biolabs, Ipswich, MA, USA) for 30 cycles with combinations of “universal” eukaryotic primers (De Jonckheere and Brown ...
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bioRxiv - Genetics 2024Quote: ... PCR amplification was performed in 50 μL reactions as follows: 25 μL 2x Q5 High Fidelity master mix (New England Biolabs Inc, Ipswich, MA, USA), 5 μL each of the forward and reverse primers (500 nM final concentration) ...
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bioRxiv - Plant Biology 2021Quote: ... These PCR products were then cloned into the pCAMBIA1301 vector (linearized by PstI and NcoI restriction enzymes, New England BioLabs, cat. no. R3140 and cat. no. R3193) using the In-fusion (Takara ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... USA) and used to generate sequences with the desired amino acid substitution using Q5 Site-Directed Mutagenesis Kit (NEB, via BioTek, Praha, Czech Republic). Primers were designed using NEBaseChanger (https://nebasechanger.neb.com/) ...
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bioRxiv - Molecular Biology 2022Quote: ... Second PCRs were run in duplicate in a final reaction volume of 50 µL using 25 µL of Q5® Hot-Start High-Fidelity 2X Master Mix (New England Biolabs® Inc., MA, USA), 3 µL of each Illumina tag (10 µM ...
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bioRxiv - Genomics 2020Quote: ... 1.5 ug of assembled array was utilized as input for methylation reactions with the non-specific adenine EcoGII methyltransferase (New England Biolabs, high concentration stock; 2.5E4U / mL). For the naked DNA controls ...
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bioRxiv - Microbiology 2021Quote: ... Reverse transcriptase and RT-qPCR reactions were conducted using the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Ipswich, MA, United States). The reaction was performed with 50 ng of total RNA in a total volume of 20 µL ...
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bioRxiv - Molecular Biology 2020Quote: ... Second PCRs were run in duplicate in a final reaction volume of 50 µl using 25 µl of Q5® Hot-Start High-Fidelity 2X Master Mix (New England Biolabs® Inc., MA, USA), 3 µl of each Illumina tag (10 µM ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription reactions were then used as input material for NEBNext® Ultra™ II DNA library Prep Kit for Illumina® (New England Biolabs, cat. E7645L), using NEBNext Multiplex Oligos for Illumina® (Unique Dual index UMI Adaptors DNA Set 1 ...
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bioRxiv - Genomics 2023Quote: ... nuclei from 2 million MCF-7/NA12878 cells at a viability of ∼95% were resuspended in 200 μl of reaction buffer (1× NEB CutSmart buffer, 0.3 M sucrose). Nuclei were then treated with EcoGII by adding 200 U of EcoGII (NEB ...