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Citations for New England Biolabs :
3751 - 3800 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... PCR was performed in a total volume of 50 μL with 4.0 μg genomic DNA per reaction using NEBNext Ultra II Q5 Master Mix (New England Biolabs, Ipswich, MA) (98 °C for 3 min ...
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bioRxiv - Genetics 2023Quote: ... Knockout of the genes was confirmed by running PCR reactions using Q5® High-Fidelity DNA Polymerase (NEB, cat no. M0491) and the following primers on a 2.5% agarose gel:
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bioRxiv - Developmental Biology 2023Quote: ... was combined with either MEP-1 or Mi-2 PCR-amplified coding sequences in a Gibson assembly reaction using NEBuilder® HiFi DNA Assembly kit (NEB) following manufacturers protocols ...
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bioRxiv - Microbiology 2022Quote: ... The PCR reaction volume used was 50 μl: 0.2 μl of Q5® high-fidelity DNA polymerase (New England Biolabs, UK), 10 μl of Q5® reaction buffer (5X ...
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bioRxiv - Developmental Biology 2023Quote: ... a plasmid containing a full infectious clone of RV-M33 was linearized then added to an in vitro transcription reaction with Sp6 (New England Biolabs, M0207L). The resulting RNA was purified then polyadenylated (New England Biolabs ...
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bioRxiv - Bioengineering 2023Quote: ... Specific donor sequences for small edits were encoded in primers and substituted into the RT-DNA-encoding region of the ncRNA with a PCR and KLD reaction (NEB M0554). Donor sequences for larger insertions were cloned through Gibson assembly ...
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bioRxiv - Microbiology 2023Quote: ... The cDNAs were diluted 1:10 and 2 μl of each used for subsequent PCR reactions with one unit of Taq polymerase (New England Biolabs, MA), 200uM dNTPs (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... The amplified fragments containing overlapping sequences of vector pKG116 were ligated into the digested vector pKG116 using Gibson assembly reaction in NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs). After cloning ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was digested in a reaction with 30 nM single-guide RNA (sgRNA, Synthego) and 30 nM spCas9 (New England BioLabs, M0386S) after pre-incubation of the reaction mix at room temperature for 10 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... 500 ng of RCA amplicons were treated with T7 endonuclease 5 μL of 10× reaction buffer and 1 μL of T7 endonuclease I (New England Biolabs, M0302S) in a 50 μL reaction volume ...
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bioRxiv - Genetics 2023Quote: ... at 37°C overnight while shaking to digest chromatin, then ligation incubation buffer (10mg/mL BSA, 1x T4 DNA Ligase Reaction Buffer (NEB, #B0202S) and 2000U of T4 DNA Ligase (NEB ...
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bioRxiv - Genomics 2023Quote: ... Included in the gel as controls were an RT reaction conducted in the absence of tRNA and a Small Range RNA Ladder (NEB N0364S). The gel was stained with SYBR gold and visualized using a ChemiDoc imager (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... as wildtype or T205A variant were amplified by polymerase chain reaction (PCR) using Q5 High-Fidelity master mix (M0492, New England Biolabs (NEB)) from previous plasmid constructs 16 ...
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bioRxiv - Neuroscience 2023Quote: ... Mice were genotyped by polymerase chain reaction (PCR) using isopropanol-precipitated genomic DNA as template and OneTaq DNA polymerase (New England Biolabs, (NEB), #M0480 ...
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bioRxiv - Microbiology 2023Quote: ... 1990) tagged with Illumina adapters in PCR reactions using a Q5® Hot Start High-Fidelity DNA Polymerase (New England Biolabs). The 25 amplification cycles were as follows ...
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bioRxiv - Genomics 2023Quote: Nuclei were resuspended in 200 uL of Methylation Reaction Buffer (Buffer M containing 1mM S-adenosylmethionine (SAM)) (New England BioLabs B9003S). 10uL high-concentration EcoGII was added per 1e6 nuclei and the nuclei suspension was incubated at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... paired oligonucleotides were incubated at 37°C for 30 min in a reaction mixture supplemented with T4 PNK (NEB, Massachusetts, USA) and T4 Ligation Buffer (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... The ADT-derived cDNAs contained in the supernatant were further purified (see SI Appendix) and used as template in a PCR reaction with the NEBNext® High Fidelity Master Mix (NEB), a Truseq small RNA RPIx (containing i7 index ...
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bioRxiv - Biochemistry 2023Quote: ... cDNA synthesis and qRT-PCR were performed in a one-pot reaction using Luna® Universal One-Step RT-qPCR Kit (NEB). A QuantStudio Real-Time PCR System (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... The specimens were then incubated with a 20-fold diluted Quick Ligase in 1x Quick Ligase Reaction Buffer from Quick Ligation Kit (New England Biolabs, M2200), supplemented with an additional 1 mM ATP (TAKARA Bio ...
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bioRxiv - Plant Biology 2024Quote: ... The generated level 0 tRNA-sgRNA modules were then combined via another cut-ligation reaction using BsaI-HF®v2 (New England Biolabs) into two L1 transcription units (C441001 and C441002 ...
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bioRxiv - Plant Biology 2024Quote: ... These modules were combined into L1 expression cassettes in a cut-ligation reaction via BsaI-HF®v2 (New England Biolabs) as following ...
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bioRxiv - Microbiology 2024Quote: ... 2 μL template cDNA/DNA was used in a 20 μL reaction containing 10 μL Luna® Universal qPCR Master Mix (New England Biolabs). The qPCR program was ...
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bioRxiv - Synthetic Biology 2024Quote: ... All PCR reactions were carried out in 30 μL reactions with 15 μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs), 0.2 μM of forward and reverse primers ...
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bioRxiv - Genomics 2024Quote: ... 10 µL of tagmented DNA was PCR amplified for 14 cycles in a 50 µL reaction volume using NEBNext High-Fidelity 2X PCR Master Mix (NEB # M0541S) and Nextera primers ...
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bioRxiv - Molecular Biology 2024Quote: ... the immunoprecipitated DNA was subjected to end repair and adaptor ligation reactions using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, E7645), according to the manufacturer instruction ...
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bioRxiv - Genomics 2024Quote: ... 480 ng of the sgRNA-barcode library and 230 ng of the reporter construct were mixed in separate 20 µL NEBuilder HiFi assembly reactions (NEB, E2621S) for 1 h at 50 °C ...
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bioRxiv - Cell Biology 2024Quote: The cell lysates or purified PINK1 bound to FLAG beads were incubated at 30°C for 1h with 200 units of ʎ protein phosphatase (LPP) in a total reaction of 100µL following the instructions of the manufacturer (New England Biolabs #P0753).
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bioRxiv - Cell Biology 2024Quote: ... Every 2 PCR reactions were pooled and purified in 100ul elution buffer using Monarch PCR & DNA Cleanup Kit (7:1 binding buffer, NEB#T1030L). 5ul/reaction Dynabeads M270 Streptavidin (Thermofisher#65305 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... ddRAD library preparation included an initial digestion of 300 ng of DNA in a 34 μL reaction (2 hours at 37 °C; 10 U each of SbfI and MspI, New England Biolabs Inc.). Standard Illumina adapters were ligated using 60 cycles of digestion at 37 °C (2 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... Assembly reactions were performed by a one-pot mixture that consists of Type II restriction enzymes with T4 DNA ligase (NEB, USA) as described in Sauret-Gueto et al ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.3 µl of this boiled culture was used as templates in 30 µl qPCR reactions using Luna® Universal qPCR Master Mix (New England Biolabs). qPCR quantification of RT-DNA abundance was performed using a set of three primers ...
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bioRxiv - Genetics 2024Quote: ... TLK1 and TLK2 point mutants were generated by site-directed mutagenesis reactions using the NEB Q5 site-directed mutagenesis kit (NEB, E0554S). Primers are listed in Supplementary Table 1.
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bioRxiv - Genomics 2024Quote: ... Cells were resuspended in 2ml ligation buffer 1 (1460 µl water, 400 µl 10x T4 DNA ligase reaction buffer (NEB, B0202SVIAL), 100 µl T4 DNA ligase (NEB ...
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High-resolution profiling reveals coupled transcriptional and translational regulation of transgenesbioRxiv - Synthetic Biology 2024Quote: ... 200 ng of purified product served as template in a 20 µL IVT reaction using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, E2040), fully substituting UTP with N1-methylpseudouridine-5’-triphosphate (TriLink Biotechnologies ...
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bioRxiv - Genetics 2024Quote: ... 5ug of genomic DNA was dephosphorylated in a 30ul reaction volume using 15U of Quick calf intestinal phosphatase (NEB cat #M0525) and 3ul of 10x rCutsmart Buffer (NEB B6004S) ...
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bioRxiv - Neuroscience 2024Quote: ... 12 20-μL reactions of gibson assembly were performed for 1 hour at 50C using the HiFi DNA Assembly MasterMix (NEB, E2621), with each reaction containing 184.9 ng of SacII-digested plasmid and a 10-fold molar excess of PCR-amplified insert ...
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bioRxiv - Microbiology 2024Quote: Three μL volumes of parasite culture were used in 25 μL PCR reactions with Phusion High-Fidelity DNA polymerase (NEB, M0530). Primer pairs and anticipated sizes of PCR products are indicated in Fig ...
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bioRxiv - Molecular Biology 2024Quote: ... 10µl from the finished PCR were used to add multiplexing indexes with 10ul of pre-mixed primers from Dual Index TT Set A (10x Genomics, PN-1000215) in a 50µl reaction with NEBNext® Q5 Hot Start HiFi PCR Master Mix (NEB, M0543L) cycling at 98°C – 3’ ...
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bioRxiv - Plant Biology 2024Quote: ... Assembly reactions with the p2x35S (GB0222) promoter and pTnos terminator (GB0037) using BsaI and BsmBI (New England Biolabs, Ipswich, MA, USA) as restriction enzymes in 25-cycle digestion/ligation reactions were performed essentially as described by Sarrion-Perdigones et al ...
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bioRxiv - Synthetic Biology 2024Quote: ... approximately 0.2µL of overnight culture was added to the reaction mixture containing 1.5µL 10x Taq ThermoPol® buffer (New England Biolabs, Cat# B9004S), 0.3µL 10mM dNTP mix (Thermo Scientific ...
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bioRxiv - Immunology 2024Quote: ... Primers for each were designed from PrimerBank for mouse and qRT-PCR was performed as a one step Sybr Green reaction using Luna kits (NEB, MA)
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bioRxiv - Cancer Biology 2024Quote: ... Assembly reaction was performed at 1:2 vector: insert ratio using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs #E5520) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Adaptor-ligated DNA fragments of proper size were enriched with PCR reaction using Phusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Physiology 2024Quote: ... the following reagents were added to each tube containing 1 cell in ∼5 μL: 2 μL M-MuLV Reverse Transcriptase Reaction Buffer (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... A PCR reaction was performed with 50 µl of Q5 Hot-Start High-Fidelity 2X Master Mix (New England Biolabs, M0494) both primers at a final concentration of 0.5 µM ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng of each sample was pooled together and mixed with 8 µL of NEBNext Quick Ligation Reaction Buffer (NEB, #B6058S), 6 µL of RNA adaptor (RMX ...
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bioRxiv - Bioengineering 2024Quote: ... 5 uL of gDNA solution was used as template in the 50 uL PCR reaction using Q5 High-Fidelity 2X Master Mix (NEB, M0492) with the manufacturer’s protocols ...
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bioRxiv - Immunology 2024Quote: ... and Phusion® High-Fidelity PCR Master Mix with HF Buffer was used in all PCR amplification reaction (New England Biolabs). Baculovirus were generated by co-transfecting pBacPak8-BTN2A2- Fc clone with linearized baculovirus DNA (TakaraBio Catalog# 631401 ...
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bioRxiv - Biophysics 2024Quote: ... The product was then ligated via Golden Gate Assembly in an overnight ligation reaction at 37°C with the following final concentrations: 1000U T4 DNA ligase (M0202, NEB, USA), 1x T4 DNA ligase buffer ...