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Citations for New England Biolabs :
3801 - 3850 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... 5 ug of total RNA was used directly in the reaction or following optional poly-A purification with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490S). RNA was mixed with 2.5 uL of the primer pool (diluted to 400 pM/oligo ...
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bioRxiv - Genomics 2024Quote: Regions-of-interest were amplified from SsDddA-treated genomic DNA in 50 ul reactions consisting of 25 μL LongAmp Hot Start Taq 2X Master Mix (New England Biolabs, M0533L), 2 μL each of 10 μM forward and reverse primers (Extended Data Table 1) ...
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bioRxiv - Genomics 2024Quote: ... 2.5µl of eluate was transferred to a new tube and RMX ligation was carried out individually for each barcode by adding 1µl of NEBNext® Quick Ligation Reaction Buffer (NEB, B6058S), 0.75µl of RMX adapter ...
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bioRxiv - Genomics 2024Quote: ... The DNA was digested for 1 hr at 37 °C in a reaction mix that consisted of: 1.5 µL 10x CutSmart Buffer (New England Biolabs, Inc., UK), 5.0 µL ddH2O ...
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bioRxiv - Bioengineering 2024Quote: All mRNAs were generated via in vitro transcription (IVT) reactions using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs E2040S). Coding sequences were ordered as gBlocks (IDT ...
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bioRxiv - Genomics 2024Quote: ... The aliquots were then divided into 3 digest reactions of 5 M cells and digested with the NlaIII restriction enzyme (NEB, R0125L). After subsequent proximity ligation and DNA extraction ...
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bioRxiv - Genetics 2024Quote: ... The extracted cDNA was used for PCR reaction for 12 cycles using Q5 Polymerase and NEB multiplex indexing primers (NEB #E7600S). The PCR product was run on a 6% PAGE gel ...
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bioRxiv - Genetics 2024Quote: ... index [73] were then added to each reaction along with 25 µL NEBNext HiFi 2X PCR Master Mix (New England Biolabs, M0541). Samples were again vortexed on full speed for 10 seconds and briefly spun down ...
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bioRxiv - Genomics 2024Quote: We created resequencing libraries for each sample using a modified half reaction protocol for the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs Inc.), with a fragmentation time of five minutes ...
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bioRxiv - Bioengineering 2024Quote: ... Plasmid templates for in vitro transcription reactions were generated by cloning appropriate RNA G4-eGFP sequences into pRNA128A (37) using NheI and NotI (New England Biolabs, USA) restriction sites ...
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bioRxiv - Bioengineering 2024Quote: ... The WarmStart Master Mix is designated because the enzyme is inactive at lower temperatures but becomes active once the reaction solution reaches a temperature of 40°C (NEB, USA). Using the enzyme and indicator of this recent invention ...
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bioRxiv - Cancer Biology 2024Quote: The iDAb LMO2-E3 ligases fusions were generated by polymerase chain reaction (PCR) using Phusion® High-Fidelity PCR kit (NEB). pEF-VH576-L10-FLAG-CRBN-mb was used as a template to generate VH576-L10-FLAG-CRBN ...
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bioRxiv - Cell Biology 2024Quote: ... Adaptor-ligated DNA fragments of proper size were enriched with PCR reaction using Fusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Cell Biology 2024Quote: ... PCR amplification was performed in a 20 μL total volume reaction using the Q5 high fidelity DNA polymerase (New England Biolabs Inc.) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... The generation or origin of the plasmids is described in Table S1 43–45 and for the assembly reactions we used the NEBuilder HiFi Assembly cloning kit (NEB E5520S). PCR reactions were carried out using the Q5 High-Fidelity DNA polymerase (NEB MO515 ...
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bioRxiv - Genetics 2024Quote: ... We amplified the pgRNA cassette from the genomic DNA by performing three rounds of PCR using 500 ng of genomic DNA for each reaction and NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541S). We deep sequenced the purified libraries with paired-end sequencing ...
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bioRxiv - Genetics 2021Quote: ... The PCR product was inserted to the PstI and BglII sites on the pWALIUM20 vector (Perkins 2015) by Gibson-assembly (NEB HiFi DNA Assembly Kit) to generate pWALEXA20 (White-AttB-LexAop for shRNA expression) ...
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bioRxiv - Systems Biology 2022Quote: ... 100 ng total RNA were used as input for ribosomal RNA (rRNA) depletion using the NEBNext rRNA depletion kit (New England BioLabs inc., product code: E6350) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: PCR products were loaded into a single lane on a 1% agarose gel with 100 bp DNA ladder (New England Biolabs, Frankfurt am Main, Germany). Fragments between 200 and 500 bp were cut from the gel with a scalpel and purified using the QIAquick gel extraction kit (QIAgen ...
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bioRxiv - Genetics 2020Quote: ... Purified PCR products were used as templates for in vitro transcription using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, New England Biolabs). Cas9 mRNA and gRNAs were then purified using RNAClean XP(Beckman Coulter ...
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bioRxiv - Bioengineering 2022Quote: ... and 15ul BseYI-digested product were run side-by-side on gel (1% agarose gel in TAE buffer, 1 kb ladder (NEB, Cat. No. / ID: N3232L), most gels run at 115V for 40 minutes) ...
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bioRxiv - Neuroscience 2023Quote: ... 50 ng total RNA were used as input for ribosomal RNA (rRNA) depletion using the NEBNext rRNA depletion kit (New England BioLabs inc., product code: E6350) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 50 ng of clean RNA product was then ligated to the RTA adapter (ONT, SQK-RNA002) using concentrated T4 DNA Ligase (NEB, M0202M, 2,000,000 units/mL) at room temperature for 10 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... the corresponding single and multiple amino acid substitutions were introduced by QuikChange site-directed mutagenesis (NEB, Phusion High-Fidelity PCR Kit) followed by KLD (Kinase ...
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bioRxiv - Neuroscience 2020Quote: ... amino acid sequence identical to GenBank: BC002453.2) was amplified using Q5 High-Fidelity DNA Polymerase (New England Biolabs, Ipswich MA, USA) and inserted into the TOPO cloning site in pET151/D-TOPO E ...
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bioRxiv - Molecular Biology 2021Quote: ... or more amino acid substitutions in SecPH with Q5 PCR methodology (Q5®Site-Directed Mutagenesis Kit, New England Biolabs, E0554) or PCR site-directed mutagenesis strategy ...
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bioRxiv - Immunology 2021Quote: ... were generated by introducing the corresponding amino acid mutations (Extended Data Fig. 5) using the Q5® Site-Directed Mutagenesis Kit (NEB) and per manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... The OprCAA mutant was produced by changing the key amino acids Cys143 and Met147 to alanine residues using the KLD Quickchange site-directed mutagenesis kit (New England Biolabs, UK) and specific primers containing both mutation sites (forward ...
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bioRxiv - Cell Biology 2022Quote: ... 6 μg of digested nucleic acids were treated overnight at 37 °C with or without 10 μl of RNase H (New England BioLabs, M029L) in 1x RNase H buffer and 1/10th of the samples was saved as input ...
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bioRxiv - Molecular Biology 2024Quote: ... STK19 was generated by deleting the first 110 amino acids of the misannotated STK19 using Q5 site directed mutagenesis (NEB, E0554S) and addition of GFP was done using In-Fusion system (Takara 639649).
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bioRxiv - Biochemistry 2024Quote: ... A truncated version of this wild-type VgrS lacking the first 81 amino acids was created by Q5-mutagenesis (NewEngland Biolabs, NEB) to generate VgrS (residues 81-729 ...
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bioRxiv - Microbiology 2020Quote: ... bacilliformis total RNA (for each 25 µl reaction) collected from various conditions using the Luna Universal One-Step RT-qPCR kit (New England BioLabs; Ipswich, MA) according to the manufacturer ...
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bioRxiv - Cancer Biology 2021Quote: ... Kras cDNA was amplified in PCR reaction using the corresponding primers (Reagents) and Phusion Hot Start Flex polymerase (New England Biolabs, Ipswich, MA). cDNA fragments were purified with NucleoSpin gel and PCR clean-up columns (Macherey-Nagel ...
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bioRxiv - Evolutionary Biology 2021Quote: ... ∼500 ng of genomic DNA was digested for 3 hours in a 50 μl reaction with 100 units each of the restriction enzymes EcoRI-HF and Msp1 (New England Biolabs, Ipswich, MA), and 1X CutSmart Buffer (New England Biolabs) ...
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bioRxiv - Immunology 2021Quote: ... 45 μl of cDNA from the synthesis reaction was mixed with primers and Q5® High-Fidelity 2X Master Mix(NEB, USA). The PCR program began with an initial denaturation at 95°C for 1.5minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μg of purified DNA from a cleavage reaction was end-repaired and dA-tailed using the NEBNext® Ultra™ II End Repair/dA-Tailing Module (New England Biolabs). The DNA was purified and ligated with the dT-tailed chloramphenicol cassette ...
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bioRxiv - Molecular Biology 2020Quote: ... 25 μL of the pooled library was mixed with 3.5 μL NEBNext Ultra II End-Prep Reaction buffer and 1.5 μL Ultra II End-prep Enzyme mix (New England Biolabs, Ipswich, MA, USA), incubated for 10 minutes at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Assembly was performed with 1650 ng of BsmBI-digested pLentiCRISPRv2 and 250 ng of amplicon in a total reaction volume of 100 μL with HiFI DNA Assembly Mix (NEB, Ipswich MA) for 30 min at 50°C ...
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bioRxiv - Biochemistry 2020Quote: ... gRNAs were individually cloned into NF-Cas9-yDHOD(-) using DNA assembly reactions (NEBuilder HiFi DNA Assembly Master Mix, New England Biolabs®, Inc.). The TetR-DOZI-aptamer conditional knockdown system was kindly provided by Dr ...
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bioRxiv - Molecular Biology 2020Quote: ... the desired sequences pertaining to the truncated forms of the N protein were subcloned from the synthetic gene using polymerase chain reaction (Phusion polymerase, New England Biolabs, Hertfordshire, UK). All genes were cloned into the modified pet28 vector and gene sequences were confirmed by Sanger Sequencing.
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bioRxiv - Neuroscience 2021Quote: ... One μg of RNA was reverse transcribed in a 20 μl reaction using Moloney Murine Leukemia Virus Reverse Transcriptase (New England Biolabs, Evry, France) and random primers and cDNA obtained amplified using Q5® DNA Polymerase (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Twenty-four PCR reactions (24 x 50 μl) were performed with 1 U of Q5 high-fidelity polymerase (New England Biolabs®, M0491), 200 μM dNTPs ...
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bioRxiv - Systems Biology 2021Quote: ... and then cloned into the EagI and EcoRI sites of the plasmid pJK03 with multiple 20 microliter ligation reactions (NEB T4 ligase). The libraries were transformed into 5-alpha electrocompetent cells (NEB ...
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bioRxiv - Plant Biology 2020Quote: ... 1 ∼ 1.5 μl of DNA extract was added to 20 μl of PCR reaction mix containing standard PCR buffer and 1 unit of Taq DNA polymerase (Cat # = M0273, New England Biolabs, Beverly, MA). For long-range PCR ...
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bioRxiv - Microbiology 2022Quote: ... Reverse-transcription polymerase chain reaction (RT-PCR) was performed using the Luna Universal Probe One-Step RT-qPCR kit (New England BioLabs; https://www.neb.ca). The 5 ‘ untranslated region (UTR ...
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bioRxiv - Microbiology 2022Quote: ... 50 fmol of each amplicon and 15 fmol of YCpBAC vector were assembled using a standard Gibson assembly reaction (New England Biolabs, Ipswich, MA), transformed into E ...
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bioRxiv - Plant Biology 2021Quote: The purified PCR reaction was then A-tailed with 15 units Klenow Fragment (3’ – 5’ exo-) (New England BioLabs; Ipswitch, MA, USA) in 1X NEB Buffer 2 (New England BioLabs ...
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bioRxiv - Microbiology 2020Quote: ... The products of this reaction were prepared for Illumina sequencing using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Enzyme restriction with DpnII was performed in 30 µl reaction mix (1 µl DpnII, 3 µl DpnII buffer, 10 µl purified DNA and 16 µl H2O; New England Biolabs, Ipswich, MA) and incubation at 37°C for 2h ...
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bioRxiv - Microbiology 2020Quote: ... PCRs were performed in a final volume of 20 μl including NEB Luna Universal Probe One-Step Reaction Mix and Enzyme Mix (both New England Biolabs, Herts, UK) and 5 μl of RNA sample ...