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Citations for New England Biolabs :
3651 - 3700 of 4002 citations for Hexadecanoic acid reaction products with tetraethylenepentamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... 5.25 mg of genomic DNA (gDNA) was used as template across 525 x 100 µL PCR reactions using Q5 2X Master Mix (NEB, M0492L). For the distal sub-library screens ...
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bioRxiv - Neuroscience 2020Quote: ... 10 μl of the reaction was directly used for restriction digest using 4 units of BssHII enzyme (New England Biolabs, USA) in a 20 μl reaction ...
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bioRxiv - Microbiology 2020Quote: ... PCR was carried out using NEB Luna Universal Probe One-Step Reaction Mix and Enzyme Mix (New England Biolabs, Herts, UK), primers and probe at 500 nM and 127.5 nM ...
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bioRxiv - Microbiology 2020Quote: ... and in vitro bacterial cultures was used as template in reverse transcription reactions using the Murine Moloney Leukemia Virus Reverse Transcriptase (NEB M0253S) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: PCR of the tagged DNA was performed in 25 μl reactions in 200 μl thin-walled PCR tubes containing 1X Q5® Buffer (NEB), 1X Q5® GC Enhancer (NEB) ...
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bioRxiv - Neuroscience 2020Quote: Overlapping fragments of the unstable NaV1.1 cassette-3 were amplified in polymerase chain reactions (PCR) using Q5® Hot Start High Fidelity 2x Master mix (New England Biolabs) and the primer pairs listed in Table S1 ...
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bioRxiv - Genomics 2021Quote: 500 ng of genomic DNA obtained after I-SceI/XbaI digestion was used as a template in a 50 μL of Q5 DNA polymerase reaction mix (NEB, M0491S), using 50 nM of primers U1 and U2 in the following cycling conditions ...
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bioRxiv - Genomics 2021Quote: 500 ng of genomic DNA obtained after I-SceI/XbaI digestion was used as a template in a 50 μL of Q5 DNA polymerase reaction mix (NEB, M0491S), using 50 nM of a UMI-containing primer U1 in the following cycling conditions ...
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bioRxiv - Cell Biology 2021Quote: The recovery probe was prepared as follows: A standard 100 µl PCR reaction was prepared containing 10 ng of bacteriophage λ DNA (NEB), 5 units of Taq polymerase (NEB) ...
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bioRxiv - Cancer Biology 2020Quote: ... 50-100 ng of gDNA was amplified in 50 µl reaction using Q5® Hot Start High-Fidelity 2X Master Mix (New England BioLabs) with initial denaturation at 98°C for 30 sec followed by 35 cycles of 98°C 10 sec ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was stored short-term at −20°C or long-term at −150°C or immediately used in a retrotranscription reaction (ProtoScript® AMV First Strand cDNA Synthesis Kit, New England Biolabs or SuperScript® III First-Strand Synthesis System ...
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bioRxiv - Biochemistry 2020Quote: ... Novel mutants of Ecm2 were generated using inverse polymerase chain reaction (PCR) with Phusion DNA polymerase (New England Biolabs; Ipswich, MA). All plasmids were confirmed by sequencing.
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bioRxiv - Microbiology 2020Quote: ... a colony of each isolate was resuspended in 50 µL of sterile water then 1 µL of resuspended bacteria was used in a PCR reaction using Phusion® High-Fidelity DNA Polymerase (New England Biolabs) with forward (16s-8F ...
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bioRxiv - Systems Biology 2021Quote: ... for each oligo pool we used 50 femtomoles of template and 4 cycles of PCR in each of multiple 50 microliter reactions (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Systems Biology 2020Quote: ... the samples were then incubated with 20-fold diluted Quick Ligase in 1× Quick Ligase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with additional 1 mM ATP (NEB P0756 ...
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bioRxiv - Microbiology 2021Quote: All PCR assays were carried out in 25 μL total reaction volumes using 2x Mastermix (New England Biolabs Inc., Ipswich, USA), 1.25 μL oligonucleotide primes (10 μM ...
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bioRxiv - Systems Biology 2021Quote: ... The samples were then incubated with 20-fold diluted Quick Ligase in 1× Quick Ligase Reaction Buffer from Quick Ligation Kit (NEB M2200) supplemented with an additional 1 mM ATP (NEB P0756 ...
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bioRxiv - Microbiology 2021Quote: ... Zip codes were then amplified by polymerase chain reaction (PCR) from 200 ng of DNA template using Phusion ® High-Fidelity (HF) DNA Polymerase (New England Biolabs) in HF Buffer ...
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bioRxiv - Plant Biology 2021Quote: ... The pooled PCR reactions were purified with the Monarch® PCR & DNA Cleanup Kit (5 μg) (New England Biolabs® Inc.) and run on 6% acrylamide gel ...
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bioRxiv - Microbiology 2021Quote: ... LAMP (Loop-Mediated Isothermal Amplification) reactions were performed with WarmStart® Colorimetric LAMP 2X Master Mix (DNA & RNA) (New England Biolabs) and an alternative set of primers (Supplementary Table S3) ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µg of GST-beads were incubated for 15 min on ice in GRB buffer in a 25 μl reaction volume with 1 µg of 100 bp DNA ladder (New England Biolabs # N3231S) or mononucleosome (Active motif # 81070) ...
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bioRxiv - Cancer Biology 2020Quote: ... we performed eight 100 μl PCR reactions per sample (4 μg DNA per reaction, 32 μg per mouse) using Q5 High-Fidelity 2x Master Mix (New England Biolabs, M0494X). The PCR products were purified with Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2021Quote: ... 100 ng total RNA per 20 µl reaction were analyzed using the Luna Universal Probe One-Step RT-qPCR Kit (NEB, # E3006) in technical triplicates ...
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bioRxiv - Cell Biology 2021Quote: ... Amplified cDNA fragments and HindIII-digested pFLAG-CMV-6a were subjected to the In-Fusion or NEBuilder reaction (New England BioLabs, E2621).
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bioRxiv - Cell Biology 2021Quote: ... neon-Green and mScarlet, these fragments were amplified by Polymerase Chain Reaction using Phusion® High-Fidelity DNA Polymerase (M0530L, New England Biolabs (NEB)) using 35 cycles with an annealing temperature of 60 °C (see table 2 for list of primers ...
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bioRxiv - Biochemistry 2022Quote: ... and 4.5 μM biotinylated primer oligo IF238 (5/Biosg/TC TCC TCC TTC T) were annealed in T4 ligase reaction buffer (NEB B0202S). The mixture was heated to 75°C for 5 min and cooled to 4°C at a rate of −1°C min−1 ...
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bioRxiv - Genomics 2022Quote: ... The Hi-TrAC libraries were then amplified with multiplexing indexed primers in the following reaction mixture: 20 μL Phusion HF PCR Master Mix (NEB, M0531S), 1 μL Illumina Multiplexing PCR primer 1.0 (10 μM) ...
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bioRxiv - Genomics 2022Quote: ... Proximity barcode ligations were carried out in 150 µl T4 ligation reaction mix (11 µL T4 ligase (NEB cat# M0437B-BM), 75 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... A reaction was carried out of the enzyme and its corresponding trisaccharide dissolved in 1X GlycoBuffer 1 (New England Biolabs, n.d.), deionized water ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μl of cells were used in a PCR reaction to obtain the gene for Sanger sequencing using Q5 (NEB, M0492). The PCR reaction was sent to Macrogen Europe B.V ...
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bioRxiv - Molecular Biology 2022Quote: ... primers pM102F and pM102R (Supplementary Table 2) were designed and used in a long-range PCR reaction using the LongAmp® Taq DNA Polymerase (NEB) to amplify 40 ng of genomic DNA following the kit instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... and assembled by Gibson Assembly (Gibson et al., 2009) reaction according to the manufacturer’s instructions (Gibson Assembly Master Mix, New England Biolabs, Ipswich, USA). Two microliters of the assembly reaction were taken for the consequent bacterial transformation.
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bioRxiv - Microbiology 2022Quote: ... The V4 region of the 16S rRNA gene was amplified from approximately 5 ng of extracted DNA in 25μl reactions using Q5 HS High-Fidelity polymerase (New England BioLabs, Ipswich, MA) with inline bare primer design as previously described.51 The following V4-specific primers were used ...
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bioRxiv - Microbiology 2020Quote: ... The products were gel purified and ligated to an XhoI and Kpn2I digested and gel purified pCVB3-XhoI-ΔP1-Kpn2I using NEBuilder® HiFi DNA Assembly reaction (NEB) for 25 minutes ...
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bioRxiv - Neuroscience 2021Quote: NRSF/REST fragments as well as the linearised backbone vector were assembled in an isothermal Gibson assembly reaction (Gibson Assembly® Cloning Kit, New England BioLabs) following the manufacturer’s instructions (Figure S4C ...
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bioRxiv - Bioengineering 2022Quote: ... Genes encoding individual components of biosensors were amplified by polymerase chain reaction (PCR) using Q5® High-Fidelity DNA Polymerase (New England Biolabs). Site-directed mutagenesis was performed by QuikChange lightning mutagenesis kit (Agilent ...
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bioRxiv - Cancer Biology 2022Quote: ... sgRNA cassettes were amplified from 2.5 µg of the template in 50-μL reactions using OneTaq® DNA Polymerase (NEB; #M0480) and LG.Lib.ampl1.F and LG.Lib.ampl1.R primers (Supplementary Table ST5) ...
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bioRxiv - Genomics 2022Quote: ... we performed two separate 100 ul reactions (25 cycles each) with 250 ng of input gDNA using Q5 MASTER MIX (NEB #M0491) and the resulting products were pooled (PCR reaction ...
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bioRxiv - Synthetic Biology 2020Quote: ... The genes of interest were amplified by polymerase chain reaction (PCR) using Q5 high fidelity DNA polymerase (New England BioLabs, USA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was subjected to end repair and dA-tailing reaction using NEBNext End repair/dA-tailing module (NEB, cat. n°E7546S) following the manufacturer’s instruction and incubated for 5 min at 20°C and then 5 min at 65°C ...
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bioRxiv - Bioengineering 2020Quote: ... 200-500 ng of the purified PCR product was used as DNA template in 25 μl of coupled in vitro transcription and translation reaction using PURExpress In Vitro Protein Synthesis Kit (New England Biolabs, E6800L). The reaction was incubated at 37°C for 30 minutes ...
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bioRxiv - Synthetic Biology 2021Quote: ... 2 μL of this reaction mix was then used to transform 12.5 μL of chemically competent DH10-β cells (New England Biolabs, C3019) for further experiments ...
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bioRxiv - Microbiology 2020Quote: ... per the manufacturer’s directions and then subjected to qRT-PCR using a single step reaction using Luna reagent (NEB, IPswhich MA). The primers and probes (5’-6FAM and Iowa Black Quencher ...
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bioRxiv - Biochemistry 2020Quote: ... chromatin and chromatin bound fractions were released from magnetic beads using binding buffer containing 5 mM CaCl2 plus an excess (2000 units/ 20 mL reaction) of micrococcal nuclease (MNase; NEB, M0247S) Beads were incubated for 5 minutes at 37 °C with shaking (1250 rpm) ...
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bioRxiv - Biochemistry 2020Quote: ... in which primers designed to bridge the deleted sequence as described in Figure S1C were used in a PCR reaction with Q5® High-Fidelity DNA Polymerase (New England Biolabs) to amplify the modified vector ...
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bioRxiv - Genetics 2020Quote: ... Each reaction was run in 10μL volumes and included 1x Luna Universal qPCR Master Mix (New England BioLabs, Ipswich, Mass. USA), optimal forward and reverse concentrations were experimentally determined (200nM for both forward and reverse primers for 17S ...
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bioRxiv - Genetics 2020Quote: ... 1μl of S2R+ or fly genomic DNA was used in a PCR reaction using Q5 High-Fidelity DNA Polymerase (NEB M0491L). Primer pairs (Supplemental File 2 ...
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bioRxiv - Biochemistry 2021Quote: Nucleosome remodeling reactions (25 μL) were carried out in REA Buffer with 1 μL of PstI (NEB, at 100,000 U/mL), 2 mM ATP ...
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bioRxiv - Synthetic Biology 2021Quote: minD,minE and FtsA genes were amplified by standard polymerase chain reaction (PCR) amplified using Phusion High-Fidelity DNA polymerase (New England Biolabs, USA) as previously reported24,30 ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: sgDNA template was generated using gene-specific oligonucleotides and constant oligonucleotide by polymerase chain reaction for 30 cycles using Q5 high fidelity polymerase (NEB, USA). PCR product was further purified using Magbio Hiprep PCR cleanup system (Magbiogenomics ...