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Citations for New England Biolabs :
351 - 400 of 1372 citations for IGFBP2 Human T7 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: sgRNAs were produced using the transcription T7 high yield kit (NEB) and purified with the EZNA micro-elute RNA clean up kit (Omega Bio-tek) ...
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bioRxiv - Bioengineering 2023Quote: ... and confirmed sequences were transformed into T7 SHuffle (New England Biolabs) competent E ...
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bioRxiv - Biochemistry 2023Quote: ... with the indicated concentration of T7 RNA polymerase (New England Biolabs) FCE ...
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bioRxiv - Microbiology 2020Quote: ... we performed a double digestion with Exonuclease T7 and Exonuclease VII (NEB) (Figure 2-figure supplement 1) ...
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bioRxiv - Developmental Biology 2020Quote: ... Heterozygosity was assessed with T7 endonuclease I cleavage (New England Biolabs, #M0302S) upon direct incubation of 10 μl amplicon ...
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bioRxiv - Neuroscience 2022Quote: In vitro transcription was performed using HiScribe Quick T7 kit (NEB E2050S) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... In vitro transcription was performed using HiScribe Quick T7 kit (NEB E2050S) following the manufacturer’s directions ...
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bioRxiv - Molecular Biology 2021Quote: ... using HiScribe™ T7 High yield RNA Synthesis kit (New England Biolabs).
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bioRxiv - Molecular Biology 2021Quote: ... digestion and then in vitro transcribed using HiScribe T7 Transcription Kit (NEB). The RNA transcripts were extracted using Trizol (Thermo ...
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bioRxiv - Pathology 2019Quote: ... 1 µl of either SP6 or T7 polymerase (New England Biolabs, USA). SP6 reactions were incubated at 40°C for 2 hours ...
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bioRxiv - Developmental Biology 2019Quote: ... The PCR products were digested with T7 Endonuclease I (T7E1, NEB M0302S) according to the recommended protocol36 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We then used HiScribe™ T7 High Yield RNA Synthesis Kit (NEB) to produce tRNALeu ...
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bioRxiv - Biophysics 2021Quote: ... for ybbR-luci-ybbR and GR-LBD T7 express (New England Biolabs), for HtpG E ...
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bioRxiv - Biochemistry 2020Quote: ... coli strain T7 Express lysY/Iq (New England Biolabs, Ipswich, MA, USA) and purified by Ni- ...
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bioRxiv - Biochemistry 2021Quote: ... coli BL21 T7 Express lysY/Iq cells (New England BioLabs, cat. # C3013). The bacterial cultures were grown at 37 °C to an optical density (OD600 ...
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bioRxiv - Biochemistry 2021Quote: RNAs were transcribed in vitro using T7 RNA Polymerase (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM CTP and 0.6 μl T7 polymerase mix (New England Biolabs.) This target mix was then loaded on to the chip by placing 10 μL of the mix on the sticky side of the PCR film (MicroAmp ...
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bioRxiv - Biochemistry 2022Quote: ... Each protein was expressed in T7 Express cells (New England BioLabs, MA) grown in Terrific Broth at 37°C following induction with 0.5mM IPTG ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were expressed in Escherichia coli T7 Express (New England Biolabs) and grown in Luria-Bertani medium ...
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bioRxiv - Evolutionary Biology 2022Quote: ... sgRNA was synthesized using HiScribe T7 High Yield RNA Synthesis Kit (NEB). The PCR fragment present the T7 promoter at 5’ end and the sequence from sgRNA of S ...
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bioRxiv - Immunology 2020Quote: ... 1.4 μg PCR products were mixed with 50U T7 RNA polymerase (NEB), 1 U RNase inhibitor (NEB) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cut efficiency of sgRNA was tested by T7 Endonuclease I (NEB #M0302L) assay on the DNA of infected cells ...
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bioRxiv - Biophysics 2021Quote: ... RNA was generated using the HiScribe T7 Quick kit (New England Biolabs) and extracted using trizol and isopropanol precipitation ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR templates for the dsRNA were prepared using T7 Megascript Kit (NEB). S2R+ cells were seeded at the density of 106 cells/ml in serum-free medium and 15 µg/ml of dsRNA was added to the cells ...
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bioRxiv - Cell Biology 2019Quote: ... 4µl (or 200Units) of T3 (or T7) RNA polymerase (NEB cat. M0378S) added and incubated at 37°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... and 100 ng of in vitro transcribed luciferase DNA (NEB T7 HiScribe) was added to the aqueous fractions during isopropanol precipitation (52) ...
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bioRxiv - Microbiology 2020Quote: Escherichia coli BL21 (DE3) pRIL and SHuffle T7 (New England Biolabs, USA) strains were routinely grown in Luria Bertani (LB) ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM CTP and 0.6 μl T7 polymerase mix (New England Biolabs)
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Importin α/β Promote Kif18B Microtubule Association to Spatially Control Microtubule DestabilizationbioRxiv - Cell Biology 2022Quote: ... plasmids were transformed into SHuffle T7 Express LysY bacteria (NEB Cat# C3030J) and induced with 0.4 mM of IPTG at 16°C for 24h ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR products were transcribed with T7 HiScribe kit (New England Biolabs) and transfected together with TrueCas9 protein v2 (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... and in vitro transcription was performed with T7 RNA polymerase (NEB; M0251) and a digoxigenin RNA labelling kit (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... The thiolated spike-ins were made using T7 RNA polymerase (NEB M0251) on BamHI-digested templates ...
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bioRxiv - Biochemistry 2023Quote: ... Mutagenesis reactions were transformed into T7 express bacterial cells (New England Biolabs) and plated on LB-Amp agar plates ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR templates for the dsRNA were prepared using T7 megascript Kit (NEB). dsRNA against bacterial β-galactosidase gene (lacZ ...
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bioRxiv - Molecular Biology 2023Quote: ... 400ng of gDNA was used for T7 endonuclease assay (NEB Cat#M0302). Assays were run in TBE polyacrylamide gels and visualized using Gel Red.
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bioRxiv - Molecular Biology 2023Quote: ... E.coli T7 express competent cells (C2566 New England Biolabs Inc., Ipswich, MA) were transformed with pLB047 ...
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bioRxiv - Biophysics 2023Quote: ... coli σ70 (σ70) was overexpressed in T7 Express cells (New England Biolabs) as inclusion bodies from the pET-28a-σ70 overexpression plasmid39 by growing the cells at 37oC to an OD600 of ∼ 0.8 ...
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bioRxiv - Molecular Biology 2023Quote: RNA libraries were synthesized by run off IVT using T7 polymerase (NEB) according to the manufacture’s protocol followed by treatment with RQ1 DNase (Promega ...
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bioRxiv - Evolutionary Biology 2023Quote: ... WMISH probes were generated via a SP6 or T7 transcription kit (NEB) and prospectively labelled with either Fluorescein or DIG-UTP ...
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bioRxiv - Microbiology 2023Quote: ... the plasmids were transformed into SHuffle T7 Express (New England Biolabs, USA) containing the plasmid pRARE extracted from Rosetta-gami B(DE3 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs) was used for in vitro transcription according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: dsRNA was generated by in vitro transcription (NEB T7 E2050s transcription kit) from PCR products generated by the following forward and reverse primers (separated by comma):
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bioRxiv - Evolutionary Biology 2019Quote: ... The two fragments were assembled using HI-FI assembly (NEB E5520S). To construct the Prcan-1-R1∷mCherry and Prcan-1-R2∷mCherry plasmids ...
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bioRxiv - Immunology 2021Quote: ... The fragments were assembled using Hi-Fi DNA Assembly Mix (NEB). For the DonorgRNA vector ...
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bioRxiv - Genomics 2021Quote: ... and 25 µl 2x NEBNext Hi-Fi PCR mix (NEB, USA) per reaction ...
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bioRxiv - Biochemistry 2021Quote: ... Nb35–His (10 μg/mL) and apyrase (25 mU/mL, NEB); the suspension was incubated for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Nb35–His (10 µg/ml) and apyrase (25 mU/ml, NEB). The suspension was incubated for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: His-MBP-Fbp17SH3 was precipitated by amylose beads (NEB; cat#E8021L). After purification ...
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bioRxiv - Plant Biology 2022Quote: ... and MBP-HIS-GFP proteins were purified with amylose resin (NEB) and SUMO-HIS-BIN2 protein was purified with Ni-NTA Agarose (Qiagen).
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bioRxiv - Genetics 2023Quote: ... To facilitate homology directed assembly (Gibson or NEB Hi Fi assembly), 30bp homology with the preferred SEED cassettes were included at each site of the BsaI sites ...