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Citations for New England Biolabs :
601 - 650 of 1372 citations for IGFBP2 Human T7 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The sgRNAs were in vitro synthesized with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). Transcription reactions were treated with RNase-free DNaseI (NEB ...
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bioRxiv - Immunology 2021Quote: ... the mRNA was synthesized following the HiScribeTM T7 ARCA mRNA Kit (with tailing) (New England Biolabs). Once the mRNA was synthesized ...
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bioRxiv - Developmental Biology 2020Quote: Double-stranded RNA (dsRNA) was produced by simultaneous transcription with T7 RNA polymerase (New England Biolabs) on PCR products containing T7 promoter sequences (CGACTCACTATAGGG ...
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bioRxiv - Developmental Biology 2020Quote: ... sgRNAs were in vitro transcribed using the HiScribe T7 Quick High Yield RNA synthesis kit (NEB) using 75 ng of template ...
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bioRxiv - Molecular Biology 2022Quote: ... gel purified and in vitro transcribed using the HiScribe T7 high yield RNA synthesis kit (NEB) in a 20 μl reaction for 10 h at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... or the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, USA) was used to transcribe the templates ...
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bioRxiv - Genetics 2019Quote: ... The reannealed amplicon was then digested with 10 units of T7 Endonuclease I (New England Biolabs) at 37 °C for 90min ...
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bioRxiv - Molecular Biology 2019Quote: m7G-capped RNA substrates 90 to 1400 nucleotides long were generated by T7 RNA polymerase (NEB) transcription of different DNA templates generated from an FLuc plasmid ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 μg of linearised DNA was then used in a HiScribe T7 synthesis kit (NEB), before DNase treatment and purification using a PureLink RNA mini kit (Thermo Fisher).
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bioRxiv - Developmental Biology 2020Quote: ... Dppa3 and mutant Dppa3 (R107E) mRNA were synthesized using HiScribe T7 ARCA mRNA kit (NEB, E2060S), and purified using RNeasy mini kit (QIAGEN ...
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bioRxiv - Immunology 2019Quote: ... and subjected to in vitro transcription (IVT) using HiScribe T7 High Yield RNA Synthesis Kit (NEB), then treated with ExoSAP-IT (Affymetrix 78200) ...
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bioRxiv - Microbiology 2021Quote: ... 600□units of T4 DNA ligase and 75□units of T7 DNA polymerase (New England BioLabs) to prime in vitro DNA synthesis ...
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bioRxiv - Biochemistry 2020Quote: RNA was transcribed from PCR-amplified inserts using the Hiscribe T7 high yield synthesis kit (NEB). 0.1µg/µl of the RNA of interest was heated to 90°Cfor 2 mins and cooled at 4°Cfor 2 mins before adding chilled 10X RNA structure buffer (500mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... All RNA synthesis was performed using the HiScribe T7 High Yield RNA synthesis kit (NEB E2040S) according to the manufacture’s protocol ...
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bioRxiv - Bioengineering 2020Quote: IVT was carried out using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB E2050S) with the addition of RNAsin (Promega N2111) ...
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bioRxiv - Bioengineering 2021Quote: ... we performed in vitro RNA transcription using the HiScribe T7 High Yield RNA Synthesis Kit (NEB). After transcription ...
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bioRxiv - Bioengineering 2021Quote: ... The purified template DNA was mixed with an in vitro transcription mixture [T7 RNA polymerase (NEB), 50 mM MgCl2 ...
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bioRxiv - Biochemistry 2021Quote: ... RCA products were debranched using 10 units/ µg DNA of T7 Endonuclease I (New England Biolabs) for 15 min at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... 5-fold molar access of pre-annealed T7 promoter adapters and either 80 units HindIII (NEB) or 40 units SpeI (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1.4 units μL−1 T7 RNA polymerase (RNAP, NEB 2 units μL−1 RNase inhibitor (NEB), 50 nM Cas9 (NEB) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1.4 units μL−1 T7 RNA polymerase (RNAP, NEB 2 units μL−1 RNase inhibitor (NEB), 50 nM Cas9 (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... 10 ml of T7 enzyme mix and 12.5 ml of 5’GpppA cap analog (S1406S, NEB) in a final reaction volume of 100 ml for 30 min at 30°C ...
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bioRxiv - Cell Biology 2022Quote: ... and subcloned into the NdeI/NotI restriction sites of pET24 using T7 DNA ligase (NEB, M0318S) to facilitate its expression as a C-terminal 6xHIS fusion protein in E ...
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bioRxiv - Bioengineering 2022Quote: ... 1x RNA polymerase buffer and ∼1 U/µl of T7 RNA polymerase (New England Biolabs, Inc.). The samples were incubated at 37 °C for 6 hours ...
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A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testingbioRxiv - Molecular Biology 2021Quote: ... 1 μM Ultramer was annealed with 1 μM T7-3G oligonucleotide in 1x Taq Buffer (NEB) in a final volume of 10 μl by heating the reaction up to 95°C for 5 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... and in-vitro transcribed using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB E2050) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Up to 1000 ng of purified PCR product was used with the HighScribe T7 kit (NEB) and incubated overnight at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: IVT was carried out using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB E2050S) with the addition of RNAsin (Promega N2111) ...
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bioRxiv - Genetics 2020Quote: ... 10 μL re-annealed PCR product was digested with 2 units T7 Endonuclease I (NEB #M0302L) in NEBuffer 2 for 60 min at 37°C ...
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bioRxiv - Genetics 2020Quote: ... The guide RNA was synthesized by in vitro transcription with T7 RNA polymerase (New England Biolabs). The oligonucleotide templates (10 ng/μL) ...
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bioRxiv - Physiology 2020Quote: ... and T7 polymerase (50 U/μl) or T3 polymerase (50 U/μl) (NEB, Hitchin, Hertfordshire, UK) with 1 μg of linearised vector containing the ArCCKP cDNA ...
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bioRxiv - Cell Biology 2021Quote: mRNAs were synthesized by in vitro transcription (IVT) using HiScribeTM T7 ARCA mRNA Kit (NEB, E2060) according to the manufacturer’s instruction with slight modification ...
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bioRxiv - Molecular Biology 2021Quote: ... sgRNAs were generated using the HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs). Founder mice (F0 ...
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bioRxiv - Microbiology 2022Quote: All recombinant proteins were expressed in Escherichia coli T7 Express lysY/Iq cells (New England Biolabs). Except for GST-PP1γ ...
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bioRxiv - Cancer Biology 2022Quote: ... 200 ng of the purified PCR product was digested with T7 endonuclease I (New England BioLabs) as specified by the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... and 1 μg was used to generate dsRNA using a HiScribe T7 RNA synthesis kit (NEB). Reaction products were purified with the GeneJET RNA Purification Kit (ThermoFisher) ...
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bioRxiv - Cell Biology 2022Quote: ... Editing efficiency of bulk cell pools was initially confirmed by T7E1 (T7 Endonuclease 1, #M0302S, NEB) assay ...
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bioRxiv - Molecular Biology 2023Quote: ... and in vitro transcribed with the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Infectious RNA was generated by in vitro transcription using the ARCA 2X T7 master mix (NEB) with subsequent transfection in 293T cells (ATCC® CRL-3216™ ...
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bioRxiv - Cancer Biology 2023Quote: ... [32P]-labelled AdML pre-mRNA was synthesized in vitro using the T7 HiScribe Transcription Kit (NEB) using [α-32P] UTP (Perkin Elmer ...
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bioRxiv - Cell Biology 2023Quote: Amplified ssDNA library was purified with the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB). For this ...
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bioRxiv - Cell Biology 2023Quote: In vitro transcription was done using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB), following the manufacturer’s instructions for short transcripts ...
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bioRxiv - Immunology 2023Quote: The mRNAs used in this study were produced using HiScribe T7 mRNA synthesis kit (NEB, Australia) using linearized DNA produced by PCR amplification ...
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bioRxiv - Developmental Biology 2023Quote: Antisense riboprobes were transcribed using NEB T7 or T3 RNA polymerase (NEB #M0251s and Fisher #501047499) and labeled with digoxygenin (DIG ...
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bioRxiv - Developmental Biology 2023Quote: ... Complete sgDNA templates were assembled and amplified using the T7 promoter (Phusion High Fidelity, NEB E0553). sgRNA was then transcribed (MEGAshortscript T7 ...
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bioRxiv - Molecular Biology 2023Quote: crRNA (Supplementary Table 2) was synthesized using a HiScribe T7 High Yield RNA Synthesis Kit (NEB). The DNA sequences includes the T7 promoter at the 5’ end and the sequence from crRNA with the target sequence at the 3’ end ...
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bioRxiv - Molecular Biology 2023Quote: ... downstream of a T7 promoter using overlap-extension PCR with Q5 High-Fidelity DNA Polymerase (NEB) and InFusion cloning (Takara Bio) ...
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bioRxiv - Systems Biology 2023Quote: ... IVT was conducted using the HiScribe® T7 Quick High Yield RNA Synthesis Kit (NEB E2050S) - 1x T7 RNA Polymerase Mix ...
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bioRxiv - Molecular Biology 2024Quote: ... The dsDNA was then linearly amplified using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) for 15Lh at 37L°C ...
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bioRxiv - Molecular Biology 2024Quote: ... were used to generate gRNA using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB) and purified using the RNeasy Mini Kit (Qiagen).