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Citations for New England Biolabs :
251 - 300 of 1462 citations for IGFBP2 Human T7 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 0.1 μL of T7 Ligase (New England Biolabs, M0318S), 0.4 μL of Fermentas FD Eco31I (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... 4 μL of t7 polymerase (New England Biolabs #M0251S), 33 μL RNase-Free water ...
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bioRxiv - Biochemistry 2024Quote: ... coli Shuffle T7 Express cells (C3029J, New England Biolabs) to aid disulfide bond formation ...
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bioRxiv - Biophysics 2024Quote: ... coli SHuffle T7 Express (New England Biolabs, Ipswich, MA) cells were transformed with 100 ng pET32-Trx-LqhαIT and ...
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bioRxiv - Cancer Biology 2023Quote: ... along with the T7 Endonuclease I (T7EI) assay (NEB).
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bioRxiv - Genomics 2024Quote: ... The concatemers are further treated with T7 Endonuclease (NEB) to remove branches before being prepped for Nanopore sequencing.
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bioRxiv - Developmental Biology 2023Quote: ... plus the appropriate RNA Polymerase T7 or Sp6 (NEB). Probes were purified with RNA Clean Up Kit (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by T7 endonuclease I assay (New England Biolabs) to detect indel mutations ...
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bioRxiv - Cancer Biology 2023Quote: ... T7 endonuclease I enzyme was purchased (New England BioLabs) and assay was performed followed by the manufacturer’s protocol.
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bioRxiv - Biophysics 2023Quote: ... The template was then transcribed with T7 polymerase (NEB), in transcription buffer (100 mM Tris-HCl ...
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bioRxiv - Genetics 2023Quote: ... followed by T7 endonuclease I assay (New England Biolabs) assay to detect mutations ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Hiscribe T7 RNA synthesis kit (NEB #E2040S). In brief ...
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bioRxiv - Biochemistry 2023Quote: ... coli SHuffle T7 cells (New England Biolabs, Ipswich, MA) in TB medium at 17 °C ...
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bioRxiv - Cell Biology 2023Quote: ... T7 RNA polymerase and RNAPol Reaction Buffer (NEB,M0251) used in our reaction.
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... in an all-in-one T7 ligase (NEB#M0318S) based Golden-Gate assembly (37° C for 5min ...
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bioRxiv - Neuroscience 2023Quote: ... used in T7 endonuclease I activity assays (NEB E3321) as previously described (Baker et al. ...
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bioRxiv - Neuroscience 2023Quote: ... were transcribed with SP6 or T7 RNA polymerase (NEB). After transcription ...
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bioRxiv - Microbiology 2024Quote: ... and T7 DNA ligase (New England Biolabs Cat. #M0318S), then electroporated into Endura Duo electrocompetent cells (Biosearch Technologies Cat ...
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bioRxiv - Biochemistry 2024Quote: ... coli Shuffle T7 cells (New England Biolabs, MA, USA) were transformed with the pHMGWA-MaBP-Nb constructs ...
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bioRxiv - Molecular Biology 2024Quote: ... and T7 DNA ligase (3000U/µl) (New England Biolabs). The product was purified and transformed into EnduraTM DUOs Electrocompetent cells (LGC ...
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bioRxiv - Neuroscience 2024Quote: ... or HiScribe T7 ARCA mRNA Kit (New England Biolabs), evaluated spectrophotometrically and by gel electrophoresis ...
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bioRxiv - Immunology 2021Quote: ... mRNA was generated from the T7 promoter in the linearized plasmids with the HiScribe T7 ARCA mRNA kit with tailing (NEB #E2060S, Ipswich, MA). We would typically use half of a kit (10 reactions ...
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bioRxiv - Genetics 2021Quote: ... sgRNAs were synthesized directly from gBlock® DNA (IDT) templates containing the T7 promoter using the HiScribe™ T7 High Yield RNA Synthesis Kit (New England BioLabs E2050) following manufacturer’s instructions for sgRNA synthesis.
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bioRxiv - Molecular Biology 2019Quote: ... sgRNAs were synthesised directly from gBlock® (IDT) templates containing the T7 promoter using the HiScribe™ T7 high yield RNA synthesis kit (New England BioLabs®) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 200 ng of DNA was used for transcription by T7 polymerase (HiScribe™ T7 Quick High Yield RNA Synthesis Kit, NEB, Ipswich, MA, USA). Finally ...
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bioRxiv - Evolutionary Biology 2020Quote: ... was cut with Hind III and Bam HI (NEB) for 1h at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... and His-MBP-ZFC3H1 onto an Amylose resin (NEB). After extensive washing ...
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bioRxiv - Immunology 2021Quote: ... were assembled using Hi-Fi DNA Assembly Mix (NEB). For the SFFV-Cas9 vector ...
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bioRxiv - Biochemistry 2021Quote: ... Nb35–His (10µg/ml) and apyrase (25mU/ml, NEB); the suspension was incubated for 1h at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... or Hi-Fi assembly from New England BioLabs (NEB) and other basic molecular cloning approaches ...
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bioRxiv - Genomics 2021Quote: ... was then inserted by Hi-Fi assembly (NEB, E2621) using 0.025 pmols of vector and 0.05 pmols of the GFP amplicon ...
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bioRxiv - Cell Biology 2021Quote: ... using NEB Hi-Fi assembly mix (New England Biolabs). A mixture of the two guide plasmids (each at 100ng/µl ...
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bioRxiv - Developmental Biology 2023Quote: ... or Q5 Hi-Fidelity DNA Polymerase (New England Biolabs) was used ...
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bioRxiv - Biochemistry 2024Quote: ... We expressed the His-SUGCT in Lemo cells (NEB), inoculating 4L of TB with 4 mL overnight culture ...
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bioRxiv - Cancer Biology 2021Quote: ... 5U of T7 Endonuclease I (New England BioLabs® M0302L) was added and the reaction was incubated at 37°C for 20 minutes ...
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bioRxiv - Biophysics 2020Quote: ... 2.5 units of T7 RNA Polymerase (New England Biolabs #M0251S) was added to initiate the reaction ...
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bioRxiv - Genetics 2021Quote: ... T7 endonuclease 1 assay was performed following manufacturer’s protocol (NEB).
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bioRxiv - Molecular Biology 2021Quote: ... followed by in vitro transcription (HiScribe T7 polymerase, NEB #E2040S), DNA degradation (TurboDNAse ...
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bioRxiv - Molecular Biology 2022Quote: ... T7 in vitro transcription was performed using HiScribe (NEB E2050S), using PCR-generated DNA as template ...
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bioRxiv - Genomics 2019Quote: ... All reactions were pooled and debranched using T7 Endonuclease (NEB) for 2 hours at 37 C ...
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bioRxiv - Cell Biology 2019Quote: ... The HiScribe™ T7 ARCA mRNA kit (New England BioLabs) was used to transcribe PIWIL3-EGFP ...
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bioRxiv - Genomics 2021Quote: ... The resulting HMW DNA is debranched using T7 Endonuclease (NEB) and purified and size-selected using SPRI beads ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 µl T7 Endonuclease I (1 U/ul, M0302S, NEB) was added and incubated at 37°C for 1 hr ...
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bioRxiv - Bioengineering 2021Quote: ... by using the HiScribe T7 High Yield RNA Synthesis (NEB) using all nucleotide triphosphates at 10 mM except for GTP ...
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bioRxiv - Plant Biology 2021Quote: ... EcoRI/T7 RNA polymerase and HindIII/T3 RNA polymerase (NEB) generated antisense and sense probes ...
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bioRxiv - Molecular Biology 2020Quote: ... HiScribe T7 Quick Yield RNA synthesis kit (New England Biolabs) was used for the in vitro transcription and the products were purified by phenol/chloroform extraction and isopropanol precipitation ...
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bioRxiv - Physiology 2020Quote: ... and the HiScribe T7 RNA Synthesis kit (New England BioLabs) followed by RNA purification with Micro Bio-spin 6 columns (BioRad).
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bioRxiv - Immunology 2022Quote: ... The HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB) was used to transcribe RNAs in vitro following the manufacturer’s recommended protocols and the resultant RNAs were purified with an EasyPure RNA Kit (TransGen Biotech ...
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bioRxiv - Developmental Biology 2022Quote: ... Assembled oligonucleotides were transcribed using HiScribe T7 Quick (NEB, E2050S) kit ...
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bioRxiv - Developmental Biology 2022Quote: ... 5’-CTTTTGACATCCGCTTCTGC-3’ followed by a T7 endonuclease assay (NEB) to detect indels ...